Abstract: The effects of liquid medium pollen, including pure pollen of Yunyan 85, Yunyan 87 and K326 and boric acid, sucrose, calcium chloride, Put, Spd and Spm solution, on the fruit setting, seed vigor and seedling quality of MS Yunyan 85, MS Yunyan 87 and MS K326 were investigated, with the pure pollen as the control. The results showed that the effects of artificial pollination with the liquid medium pollen which contained 0.01 g calcium chloride, 100 g sucrose and 100 g pure pollen per liter was better for MS Yunyan 85, the fruit setting percentage increased by 19.98%, the 1 000-grain weight increased 2 mg, seedling dry weight increased 1.7 mg, the amount of pure pollen used decreased by 37.6%, the liquid medium pollen had no significant effect on seed number per capsule, seed weight per capsule, germination energy, germination percentage, germination index and mean germination time. For MS Yunyan 87 and MS K326, the effects of artificial pollination with liquid medium pollen containing 0.5 mmol Spd and 150 g pure pollen per liter was better, the 1 000-grain weight increased 7.4 mg and 8.4 mg respectively, dry weight of seedlings increased 0.95 mg and 0.15 mg respectively, and fruit setting percentage of MS K326 increased by 26.09%, the pure pollen amount decreased by 40.1% and 39.3%, and had no significant effect on other indexes. The results indicated that the liquid medium pollen used in tobacco seed production could save the application amount of pure pollen on the basis of ensuring the yield and quality of seed, thus reducing the seed production costs．

Key words: tobacco, liquid medium pollen, fruit setting, seed vigor, seedling