Preliminary study on measuring rice seed respiration based on TDLAS technology

doi:10.3969/j.issn.1004-1524.2021.11.18

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (11): 2155-2163.DOI: 10.3969/j.issn.1004-1524.2021.11.18

• Biosystems Engineering • Previous Articles Next Articles

Preliminary study on measuring rice seed respiration based on TDLAS technology

ZHENG Wen¹^,²(), JIA Liangquan²^,^*(), QI Hengnian², WANG Ruiqin², ZHAO Guangwu¹, YUAN Jun²

1. College of Information Engineering, Zhejiang A&F University, Hangzhou 311300,China
2. College of Information Engineering, Huzhou University, Huzhou 313000,China

Received:2020-09-02 Online:2021-11-25 Published:2021-11-26
Contact: JIA Liangquan

Abstract

Abstract:

To explore the correlation between rice seed respiration intensity and various vitality indicators, and to provide experimental basis for non-destructive testing of seed vitality through respiratory intensity measurement, a self-designed seed respiration detection system based on tunable diode laser absorption spectroscopy (TDLAS) was used, real-time changes in the concentration of CO₂ produced by seed respiration were detected. The rice seeds of Mengliangyou huanglizhan at different harvest periods were selected as the materials. The seeds in each harvest period were divided into two parts. One part was subjected to a germination test to obtain seed vigor index, and the other part was sterilized and soaked before pretreatment. The respiration intensity test was carried out in a closed environment in the seed respiration container. Each continuous test was about 10 hours, and each group of samples was repeated three times to get the average value. Rice seeds were soaked for 0, 12, 24, 36, 48 h, and it was found that the respiration intensity of the seeds increased with the soaking time within a certain soaking time. After 10 hours of continuous testing, the immersion time period with the highest respiratory intensity was 36 h, and the CO₂ concentration reached 10 151 mg·m^-3. The unsoaked dry seeds had the weakest respiratory intensity, with a CO₂ concentration of 388 mg·m^-3. Rice seed respiration intensity had a certain correlation with germination rate, germination vigor, germination index, three-day flooding germination rate, and field emergence rate. The highest correlation index could reach 0.97, 0.96, 0.97, 0.77 and 0.65. Studies showed that Mengliangyou huanglizhan rice seeds could reach a high respiration intensity after soaking for about 36 hours, and the respiration intensity increased first and then slowed down with the increase of respiration time. The respiration intensity of seeds had a high positive correlation with each vitality index.

Key words: rice, seed vigor, seed soaking time, respiration intensity, carbon dioxide, correlation

CLC Number:

S330.3

ZHENG Wen, JIA Liangquan, QI Hengnian, WANG Ruiqin, ZHAO Guangwu, YUAN Jun. Preliminary study on measuring rice seed respiration based on TDLAS technology[J]. Acta Agriculturae Zhejiangensis, 2021, 33(11): 2155-2163.

Figures/Tables 8

References 32

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编号 Number	收获时期 Harvest time	发芽率 Germination rate/%	发芽势 Germination potential/%	发芽指数 Germination index	淹水3 d发芽率 Germination rate after three days of flooding/%	田间出苗率 Field germination rate/%
A1	9月4日September 4th	87.7 a	74.0 bc	28.7 ab	85.3 a	39.0 c
A2	9月7日September 7th	79.3 b	69.7 c	26.2 b	70.7 ab	48.7 b
A3	9月9日September 9th	89.3 a	77.3 b	30.5 ab	83.3 a	50.0 b
A4	9月11日September 11th	90.3 a	85.0 a	32.1 a	90.7 a	70.0 a
A5	9月13日September 13th	86.0 a	75.7 b	28.0 ab	57.3 b	51.3 b

编号 Number	收获时期 Harvest time	发芽率 Germination rate/%	发芽势 Germination potential/%	发芽指数 Germination index	淹水3 d发芽率 Germination rate after three days of flooding/%	田间出苗率 Field germination rate/%
A1	9月4日September 4th	87.7 a	74.0 bc	28.7 ab	85.3 a	39.0 c
A2	9月7日September 7th	79.3 b	69.7 c	26.2 b	70.7 ab	48.7 b
A3	9月9日September 9th	89.3 a	77.3 b	30.5 ab	83.3 a	50.0 b
A4	9月11日September 11th	90.3 a	85.0 a	32.1 a	90.7 a	70.0 a
A5	9月13日September 13th	86.0 a	75.7 b	28.0 ab	57.3 b	51.3 b

活力指标 Vitality index	测量时间 Measure time/h										平均值 Average value
活力指标 Vitality index	1	2	3	4	5	6	7	8	9	10	平均值 Average value
发芽率Germination rate	-0.14	0.02	0.41	0.71	0.86^*	0.93^*	0.97^*	0.95^*	0.92^*	0.88^*	0.89
发芽势Germination potential	-0.10	-0.14	0.29	0.61	0.78	0.88^*	0.93^*	0.96^*	0.96^*	0.94^*	0.87
发芽指数Germination index	-0.10	-0.10	0.29	0.65	0.80^*	0.90^*	0.95^*	0.97^*	0.97^*	0.97^*	0.89
淹水3 d发芽率	-0.51	-0.53	-0.33	0.04	0.21	0.37	0.52	0.61	0.69	0.77	0.46
Germination rate after three days of flooding
田间出苗率Field germination rate	0.10	-0.13	0.17	0.38	0.49	0.55	0.57	0.62	0.64	0.65	0.56

活力指标 Vitality index	测量时间 Measure time/h										平均值 Average value
活力指标 Vitality index	1	2	3	4	5	6	7	8	9	10	平均值 Average value
发芽率Germination rate	-0.14	0.02	0.41	0.71	0.86^*	0.93^*	0.97^*	0.95^*	0.92^*	0.88^*	0.89
发芽势Germination potential	-0.10	-0.14	0.29	0.61	0.78	0.88^*	0.93^*	0.96^*	0.96^*	0.94^*	0.87
发芽指数Germination index	-0.10	-0.10	0.29	0.65	0.80^*	0.90^*	0.95^*	0.97^*	0.97^*	0.97^*	0.89
淹水3 d发芽率	-0.51	-0.53	-0.33	0.04	0.21	0.37	0.52	0.61	0.69	0.77	0.46
Germination rate after three days of flooding
田间出苗率Field germination rate	0.10	-0.13	0.17	0.38	0.49	0.55	0.57	0.62	0.64	0.65	0.56

Preliminary study on measuring rice seed respiration based on TDLAS technology

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