Genome-wide identification and expression analysis of LOX gene family in eggplant (Solanum melongena)

doi:10.3969/j.issn.1004-1524.2021.06.07

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (6): 1025-1034.DOI: 10.3969/j.issn.1004-1524.2021.06.07

• Horticultural Science • Previous Articles Next Articles

Genome-wide identification and expression analysis of LOX gene family in eggplant (Solanum melongena)

ZHAO Guofu¹(), YAN Yaqin², WANG Jinglei², WEI Qingzhen², BAO Chonglai²^,^*()

1. Department of Agricultural and Biological Engineering, Taizhou Vocational College of Science & Technology, Wenling 318020, China
2. Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2021-01-20 Online:2021-06-25 Published:2021-06-25
Contact: BAO Chonglai

Abstract

Abstract:

Lipoxygenase (LOX) is a class of non-heme iron-containing dioxygenase which plays important role in plant growth and biotic/abiotic stress tolerance.To explore LOX family genes and their evolutionary relationship in eggplants, twelve candidates, which were distributed on chromosomes 1, 3, 8, and 9, were identified as SmeLOX gene by genome-wide analysis. Phylogenetic analysis showed that SmeLOX genes were classified into three categories (9-LOX, type I 13-LOX, and type II 13-LOX). In addition, real-time quantitative PCR analysis demonstrated that only the expression of SmeLOX1, SmeLOX4, SmeLOX6, and SmeLOX8 were detected in all tissues of eggplant, including roots, stems, leaves, flowers and fruits. Overall, this study provided scientific basis for further function analysis of the SmeLOX genes during growth and development of eggplant.

Key words: eggplant (Solanum melongena), SmeLOX gene, phylogenetic analysis, gene expression

CLC Number:

S641.1

ZHAO Guofu, YAN Yaqin, WANG Jinglei, WEI Qingzhen, BAO Chonglai. Genome-wide identification and expression analysis of LOX gene family in eggplant (Solanum melongena)[J]. Acta Agriculturae Zhejiangensis, 2021, 33(6): 1025-1034.

Figures/Tables 7

References 32

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基因Gene	正向引物Forward primer	反向引物Reverse primer
SmeLOX1	TGGATCATCATGACACAGTC	GGATGAGGAAGGCTTAGTTC
SmeLOX2	ATTGAAACCGTTAGCAATTGAA	CTCAAGACCATCTTCATGTGG
SmeLOX3	AGTAACTGCAGGTGAATCAG	CAAGTGTGAGTGACTTGAGA
SmeLOX4	CCCCCACTTAAAAGAGATAGA	AGCAATTCAGGAGTTCCAAA
SmeLOX5	AGGCCTTAACATTCTCCGGC	ATCTGGTAAATGGACTCCAACG
SmeLOX6	AATCAAACATGTCTGGTCAC	TCCCTAGATTTCTCCACCAA
SmeLOX7	TAAGCAGTTTCTCCTTGACC	CTCGTCTAGAGAATGTGTCG
SmeLOX8	AATTTATGGGTATTTCCCTGC	CCTGAATGCTTCTCTTGTCC
SmeLOX9	AGATTTACCTCGGACAAAGG	CAGTATTTGGTCACCATTCC
SmeLOX10	TGAATGTGTTTCAAGGAAGTG	GCTTTTGATTTTGCATGAGTTT
SmeLOX11	GGAACCATGACACTACAGA	ACTTCGTGAAATCGATCAAG
SmeLOX12	TAGCCAGACCTTTTAACGAG	AAATTTATGCAATTGCCCCC
18S RNA	CGCGCGCTACACTGATGTATTCAA	TACAAAGGGCAGGGACGTAGTCAA

基因Gene	正向引物Forward primer	反向引物Reverse primer
SmeLOX1	TGGATCATCATGACACAGTC	GGATGAGGAAGGCTTAGTTC
SmeLOX2	ATTGAAACCGTTAGCAATTGAA	CTCAAGACCATCTTCATGTGG
SmeLOX3	AGTAACTGCAGGTGAATCAG	CAAGTGTGAGTGACTTGAGA
SmeLOX4	CCCCCACTTAAAAGAGATAGA	AGCAATTCAGGAGTTCCAAA
SmeLOX5	AGGCCTTAACATTCTCCGGC	ATCTGGTAAATGGACTCCAACG
SmeLOX6	AATCAAACATGTCTGGTCAC	TCCCTAGATTTCTCCACCAA
SmeLOX7	TAAGCAGTTTCTCCTTGACC	CTCGTCTAGAGAATGTGTCG
SmeLOX8	AATTTATGGGTATTTCCCTGC	CCTGAATGCTTCTCTTGTCC
SmeLOX9	AGATTTACCTCGGACAAAGG	CAGTATTTGGTCACCATTCC
SmeLOX10	TGAATGTGTTTCAAGGAAGTG	GCTTTTGATTTTGCATGAGTTT
SmeLOX11	GGAACCATGACACTACAGA	ACTTCGTGAAATCGATCAAG
SmeLOX12	TAGCCAGACCTTTTAACGAG	AAATTTATGCAATTGCCCCC
18S RNA	CGCGCGCTACACTGATGTATTCAA	TACAAAGGGCAGGGACGTAGTCAA

基因名称 Gene name	基因位置 Gene code	氨基酸长度 Amino acids length/aa	蛋白质相对分子质量 Molecular weight/u	芳香度 Aromaticity	不稳定指数 Instability index	等电点 Isoelectric point	亲水性 Gravy
SmeLOX1	Smechr0101045.1	843	95 774.44	0.09	40.53	5.85	-0.399
SmeLOX2	Smechr0101046.1	836	95 455.10	0.11	42.00	5.60	-0.410
SmeLOX3	Smechr0101047.1	863	97 961.05	0.10	40.19	5.45	-0.404
SmeLOX4	Smechr0104163.1	898	101 947.77	0.11	40.69	6.05	-0.353
SmeLOX5	Smechr0104167.1	899	101 887.71	0.10	37.98	6.12	-0.385
SmeLOX6	Smechr0300687.1	909	103 351.01	0.09	45.09	8.35	-0.404
SmeLOX7	Smechr0300720.1	793	90 715.48	0.09	53.28	6.14	-0.388
SmeLOX8	Smechr0303656.1	908	102 036.25	0.09	40.27	6.67	-0.349
SmeLOX9	Smechr0800437.1	859	96 846.64	0.10	39.79	5.41	-0.353
SmeLOX10	Smechr0901372.1	846	97 216.76	0.10	45.77	8.54	-0.496
SmeLOX11	Smechr0902151.1	886	100 649.19	0.10	41.35	5.89	-0.363
SmeLOX12	Smechr0902152.1	851	97 626.82	0.10	45.39	5.89	-0.433

基因名称 Gene name	基因位置 Gene code	氨基酸长度 Amino acids length/aa	蛋白质相对分子质量 Molecular weight/u	芳香度 Aromaticity	不稳定指数 Instability index	等电点 Isoelectric point	亲水性 Gravy
SmeLOX1	Smechr0101045.1	843	95 774.44	0.09	40.53	5.85	-0.399
SmeLOX2	Smechr0101046.1	836	95 455.10	0.11	42.00	5.60	-0.410
SmeLOX3	Smechr0101047.1	863	97 961.05	0.10	40.19	5.45	-0.404
SmeLOX4	Smechr0104163.1	898	101 947.77	0.11	40.69	6.05	-0.353
SmeLOX5	Smechr0104167.1	899	101 887.71	0.10	37.98	6.12	-0.385
SmeLOX6	Smechr0300687.1	909	103 351.01	0.09	45.09	8.35	-0.404
SmeLOX7	Smechr0300720.1	793	90 715.48	0.09	53.28	6.14	-0.388
SmeLOX8	Smechr0303656.1	908	102 036.25	0.09	40.27	6.67	-0.349
SmeLOX9	Smechr0800437.1	859	96 846.64	0.10	39.79	5.41	-0.353
SmeLOX10	Smechr0901372.1	846	97 216.76	0.10	45.77	8.54	-0.496
SmeLOX11	Smechr0902151.1	886	100 649.19	0.10	41.35	5.89	-0.363
SmeLOX12	Smechr0902152.1	851	97 626.82	0.10	45.39	5.89	-0.433

基因Gene	根Root	花Flower	叶片Leaf	茎秆Stem	果实Fruit
SmeLOX1	0.10±0.05 gE	1.30±0.05 eB	0.60±0.02 hC	0.30±0.09 efD	2.10±0.06 cA
SmeLOX2	0.30±0.02 fD	1.60±0.09 dA	0.50±0.04 hiC	0 fE	1.30±0.01dB
SmeLOX3	0 gC	0 hC	0.90±0.05 gB	2.01±0.04 bA	2.10±0.10 cA
SmeLOX4	1.60±0.05 dC	3.63±0.12 aA	1.20±0.03 fD	0.76±0.02 dE	2.63±0.23 bB
SmeLOX5	0 gE	2.85±0.09 bA	2.30±0.14 dC	0.72±0.03 dC	0.30±0.06 fgD
SmeLOX6	3.20±0.07 bA	0.30±0.05 fD	0.40±0.02 iD	1.69±0.16 cB	0.86±0.04 eC
SmeLOX7	3.60±0.19 aA	0.50±0.03 fC	0.20±0.04 iD	0 fE	1.23±0.08 dB
SmeLOX8	0.62±0.04 eD	3.01±0.12 bB	1.90±0.03 eC	3.70±0.27 aB	6.30±0.27 aA
SmeLOX9	0 gD	2.65±0.15 cA	1.89±0.05 eB	0.30±0.07 efC	0.46±0.05 fC
SmeLOX10	2.60±0.11 cA	0 gC	2.60±0.16 cA	0.16±0.05 fC	2.35±0.01 cB
SmeLOX11	1.50±0.02 dB	0.50±0.05 gD	4.60±0.15 aA	1.30±0.03 dC	0 hE
SmeLOX12	0 gC	0 gC	4.20±0.13 bA	0.55±0.02 efB	0.23±0.01 gC

Genome-wide identification and expression analysis of LOX gene family in eggplant (Solanum melongena)

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