Analysis of biological, physiological and biochemical mechanism of abortion of C-type cytoplasmic male sterile line S37-2 in maize

doi:10.3969/j.issn.1004-1524.2023.02.02

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (2): 259-265.DOI: 10.3969/j.issn.1004-1524.2023.02.02

• Crop Science • Previous Articles Next Articles

Analysis of biological, physiological and biochemical mechanism of abortion of C-type cytoplasmic male sterile line S37-2 in maize

ZHENG Ran¹(), LYU Dan¹, WU Qinggui¹, DI Xiaohong¹, ZHU Tongtong¹, QIU Guanjie¹, LUO Hongbing¹^,²^,^*()

1. College of Agronomy, Hunan Agricultural University, Changsha 410128, China
2. Maize Engineering Technology Research Center of Hunan Province, Changsha 410128, China

Received:2022-04-25 Online:2023-02-25 Published:2023-03-14
Contact: LUO Hongbing

Abstract

Abstract:

To reveal the physiology and biochemistry mechanism of male sterile abortion in maize, the sterile line S37-2 and fertile line B37-2 were applied as test materials in the present study. The main agronomic traits of S37-2 and B37-2 were investigated. The differences of the contents of soluble sugar, soluble protein, free proline, soluble starch, and malonaldehyde (MDA), and the activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) in leaves between S37-2 and B37-2 were determined. The results showed that S37-2 had thin and atrophic anther shape and poor plumpness, as the length and cross section diameter of anther were significantly (P<0.05) smaller than those of B37-2. The contents of soluble protein, free proline and soluble sugar in S37-2 leaves were significantly (P<0.05) higher than those of B37-2 at the tasseling stage and pollen shedding stage. During the whole growth period, the CAT and SOD activities of S37-2 were significantly (P<0.05) lower than those of B37-2 at the same growth stage. It was inferred that the blocked transportation path of soluble protein, free proline and soluble sugar in leaves of S37-2, with the relatively lower activities of SOD and CAT led to the sterility of S37-2.

Key words: maize, male sterility, phenotypic traits, physiological indicators

CLC Number:

S513
S503.3

ZHENG Ran, LYU Dan, WU Qinggui, DI Xiaohong, ZHU Tongtong, QIU Guanjie, LUO Hongbing. Analysis of biological, physiological and biochemical mechanism of abortion of C-type cytoplasmic male sterile line S37-2 in maize[J]. Acta Agriculturae Zhejiangensis, 2023, 35(2): 259-265.

Figures/Tables 5

References 34

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性状Trait	S37-2	B37-2
株高Plant height/cm	102.54±11.18 a	97.63±8.09 a
穗位高Ear height/cm	35.03±5.98 a	32.93±5.82 a
穗上位茎粗	11.78±0.56 a	11.89±0.50 a
Upper spike stem diameter/mm
茎粗Stem diameter/mm	15.85±1.41 a	15.89±1.70 a
茎节数Number of node	11.32±0.70 a	11.52±0.51 a
叶片数Number of leaf	9.59±0.55 a	9.67±0.62 a
叶长Leaf length/cm	69.34±4.26 a	66.49±2.46 b
叶宽Leaf width/cm	8.69±0.48 a	7.76±0.54 b
雄穗分枝数Number of tassel	9.03±1.87 a	8.98±1.53 a
雄穗长Tassel length/cm	26.91±1.34 a	27.30±1.13 a

性状Trait	S37-2	B37-2
株高Plant height/cm	102.54±11.18 a	97.63±8.09 a
穗位高Ear height/cm	35.03±5.98 a	32.93±5.82 a
穗上位茎粗	11.78±0.56 a	11.89±0.50 a
Upper spike stem diameter/mm
茎粗Stem diameter/mm	15.85±1.41 a	15.89±1.70 a
茎节数Number of node	11.32±0.70 a	11.52±0.51 a
叶片数Number of leaf	9.59±0.55 a	9.67±0.62 a
叶长Leaf length/cm	69.34±4.26 a	66.49±2.46 b
叶宽Leaf width/cm	8.69±0.48 a	7.76±0.54 b
雄穗分枝数Number of tassel	9.03±1.87 a	8.98±1.53 a
雄穗长Tassel length/cm	26.91±1.34 a	27.30±1.13 a

性状Trait	S37-2	B37-2
花药长度Length of anther/mm	3.52±0.18 b	4.67±0.13 a
花药横截面直径	0.40±0.03 b	1.00±0.05 a
Cross section diameter of anther/mm

性状Trait	S37-2	B37-2
花药长度Length of anther/mm	3.52±0.18 b	4.67±0.13 a
花药横截面直径	0.40±0.03 b	1.00±0.05 a
Cross section diameter of anther/mm

Analysis of biological, physiological and biochemical mechanism of abortion of C-type cytoplasmic male sterile line S37-2 in maize

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