Cloning and functional research of MYB transcription factor AsMYB44 from Angelica sinensis

doi:10.3969/j.issn.1004-1524.2023.06.03

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (6): 1253-1264.DOI: 10.3969/j.issn.1004-1524.2023.06.03

• Crop Science • Previous Articles Next Articles

Cloning and functional research of MYB transcription factor AsMYB44 from Angelica sinensis

LIU Guangrui¹^,²(), ZONG Yuan², LI Yun², CAO Dong², LIU Baolong², BAO Xuemei¹^,²^,³, LI Jianmin¹^,^*()

1. School of Life Sciences, Qinghai Normal University, Xining 810008, China
2. Qinghai Provincial Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China
3. CAS Key Laboratory of Tibetan Medicine Research, Xining 810008, China

Received:2022-07-02 Online:2023-06-25 Published:2023-07-04

Abstract

Abstract:

In order to study the mechanism of biosynthetic regulation of Angelica sinensis phenolic acids, a MYB transcription factor AsMYB44 gene was cloned from Angelica sinensis and the relevant bioinformatics analysis of the sequence was carried out using biological software. The Agrobacterium tumefaciens-mediated genetic transformation system was overexpressed in Samsun tobacco, and a multi-omics combined analysis of the transgenic tobacco was performed. The CDS (coding sequence) of the AsMYB44 was 903 bp, encoding 300 amino acids, and with the complete SANT and MYB domains. Phylogenetic analysis showed that AsMYB44 had higher homology with Arabidopsis thaliana AtMYB11 (AF062863.1), AtMYB12 (AF062864.1) and Salvia miltiorrhiza SmMYB97 (KF059561.1). The results of transcriptome sequencing showed that the differentially expressed genes were mostly enriched in the phenylpropane synthesis and metabolism pathway in AsMYB44 transgenic tobacco. Expression levels of phenylalanine ammonia lyase gene (PAL), cinnamic acid-4-hydroxyl (C4H), 4-coumaric acid: coenzyme A ligase gene (4CL), caffeic acid 3-O-methyltransferase gene (COMT), cinnamyl alcohol dehydrogenase gene (CAD) and other structural genes were upregulated. Metabolomic analysis showed that the contents of metabolites such as phenolic acids, lipids, alkaloids, flavonoids and other compounds in transgenic tobacco changed significantly. These results indicated that the AsMYB44 transcription factor could positively regulate the metabolic synthesis of phenolic acids, which provided a theoretical basis for the biosynthesis mechanism of phenolic acid secondary metabolites in Angelica sinensis.

Key words: Angelica sinensis, AsMYB44 transcription factor, gene cloning, transgenic tobacco, multi-omics analysis, phenolic acid

CLC Number:

S567.239

LIU Guangrui, ZONG Yuan, LI Yun, CAO Dong, LIU Baolong, BAO Xuemei, LI Jianmin. Cloning and functional research of MYB transcription factor AsMYB44 from Angelica sinensis[J]. Acta Agriculturae Zhejiangensis, 2023, 35(6): 1253-1264.

Figures/Tables 6

Table 1 Primer sequences

用途 Purpose	基因名称 Gene name	正向引物 Forward primer(5’→3’)	反向引物 Reverse primer(5’→3’)
实时荧光定量PCR	NtActin	AATGATCGGAATGGAAGCTG	TGGTACCACCACTGAGGACA
qRT-PCR	NtPAL	ATAGACTTGAGGCATTTGG	GCATCAGTGGGTAGTTAGC
	NtC4H	GAGGCTGAACAGAAGGGAG	TGTGGGACTAAAAGAGGGA
	Nt4CL	GTTCTCGTCCCTGTTTGAT	GAGGCTTTGGCTTGTTTTA
	NtF5H	TCCTTCTCTCCAAATCTCG	AACTTATGGCTACGGTCGC
	NtCOMT	GATGCTTAGGCTACTTGCT	TCATTGACATAGTGGAGTG
	NtCAD	CAGCGAAAACAGTATGCGT	CAGCGAAAACAGTATGCGT
	NtCCR	CAGCATCTCCATTTCCCTA	ATCCCTTTCTCCTTCACAA
基因克隆Gene cloning	AsMYB44	acgcGAGCTCATGTCCAGGAGAGATATGGATCG	ctagTCTAGACTAATCGATTTTACTAATCCCCATTC

Fig.1 Cloning, domain analysis and phylogenetic analysis of AsMYB44 gene A, AsMYB44 gene clone; M, DL5000 marker; 1-3, PCR product of Angelica sinensis leaf cDNA. B, Domain analysis. C, Phylogenetic analysis of AsMYB44.

Fig.2 PCR detection of Escherichia coli colony, Agrobacterium liquid and transgenic tobacco A, 1-10 were PCR products of E. coli colonies, 11 was PCR product with AsMYB44 plasmid as template, 12 was negative control; B, 1-9 were PCR products of Agrobacterium colonies, 10 was PCR product with AsMYB44 plasmid as template, 11 was negative control; C, 1-12 were PCR products with transgenic tobacco DNA as templates, 13 was PCR product with AsMYB44 plasmid as template, 14 was negative control; M, DL5000 marker.

Fig.3 Statistical graph, volcano graph and KEGG enrichment factor graph of differentially expressed genes A, Statistical map of differentially expressed genes; B, Volcano map of differentially expressed genes; C, Map of KEGG enrichment factors of differentially expressed genes.

Fig.4 Statistics of differential metabolite categories, KEGG pathway enrichment and multi-omics analysis of phenylpropane metabolic pathways A, Statistics of differential metabolite categories; B, KEGG pathway enrichment of differential metabolites; C, Multi-omics analysis of phenylpropane metabolic pathways.

Fig.5 Relative expression levels of some key genes in the phenylpropane metabolism pathway of AsMYB44 transgenic tobacco *represented P ≤0.05, **represented P ≤0.01, ***represented P ≤0.001, ****represented P ≤0.000 1.

References 51

[1]	李曦, 张丽宏, 王晓晓, 等. 当归化学成分及药理作用研究进展[J]. 中药材, 2013, 36(6): 1023-1028.
	LI X, ZHANG L H, WANG X X, et al. Research progress on chemical constituents and pharmacological effects of Angelica sinensis[J]. Journal of Chinese Medicinal Materials, 2013, 36(6): 1023-1028. (in Chinese)
[2]	黄伟晖, 宋纯清. 当归的化学和药理学研究进展[J]. 中国中药杂志, 2001, 26(3): 147-151.
	HUANG W H, SONG C Q. Research progress in chemistry and pharmacology of Angelica sinensis[J]. China Journal of Chinese Materia Medica, 2001, 26(3): 147-151. (in Chinese)
[3]	马艳春, 吴文轩, 胡建辉, 等. 当归的化学成分及药理作用研究进展[J]. 中医药学报, 2022, 50(1): 111-114.
	MA Y C, WU W X, HU J H, et al. Research progress on chemical constituents and pharmacological effects of Angelica sinensis[J]. Acta Chinese Medicine and Pharmacology, 2022, 50(1): 111-114. (in Chinese with English abstract)
[4]	魏明刚, 张玲, 倪莉, 等. 加味当归补血汤对阿霉素肾病大鼠肾脏足细胞保护机制的研究[J]. 中国中西医结合杂志, 2012, 32(8): 1077-1082.
	WEI M G, ZHANG L, NI L, et al. Protection mechanisms of modified Danggui Buxue Decoction for podocytes in adriamycin-induced nephropathy rats[J]. Chinese Journal of Integrated Traditional and Western Medicine, 2012, 32(8): 1077-1082. (in Chinese with English abstract)
[5]	孙玉敏, 宋福成, 吴晓光. 当归补血汤抑制荷瘤小鼠肿瘤生长的作用[J]. 现代生物医学进展, 2006, 6(9): 31-32.
	SUN Y M, SONG F C, WU X G. The inhibiting effect of Danggui Buxue Tang on tumor’s growth of tumor-bearing mice[J]. Progress in Modern Biomedicine, 2006, 6(9): 31-32. (in Chinese with English abstract)
[6]	邢利鹏. 三种均一分子量当归多糖的制备、结构鉴定及体外抗肿瘤活性研究[D]. 武汉: 华中科技大学, 2013.
	XING L P. Study on structure identification and anti-tumor activity in vitro of three uniform molecular weight-Angelica polysaccharides[D]. Wuhan: Huazhong University of Science and Technology, 2013. (in Chinese with English abstract)
[7]	叶依依, 刘胜, 郭宝凤, 等. 蛇床子素配伍补骨脂素对人乳腺癌高骨转移细胞增殖及侵袭的影响[J]. 中华中医药学刊, 2013, 31(1): 36-40.
	YE Y Y, LIU S, GUO B F, et al. Effect of osthole combined with psoralen on MDA-MB-231BO cell proliferation and invasion[J]. Chinese Archives of Traditional Chinese Medicine, 2013, 31(1): 36-40. (in Chinese with English abstract)
[8]	骆亚莉, 李应东, 刘永琦, 等. 当归有效成分对冷应激小鼠抗氧化功能影响[J]. 中国公共卫生, 2014, 30(5): 607-609.
	LUO Y L, LI Y D, LIU Y Q, et al. Effects of active fraction of Angelica sinensis on antioxidant function in cool stress mice[J]. Chinese Journal of Public Health, 2014, 30(5): 607-609. (in Chinese with English abstract)
[9]	王志新, 张志立, 李哲诚, 等. 当归补血汤加味抗肝炎肝纤维化临床研究[J]. 亚太传统医药, 2014, 10(23): 101-103.
	WANG Z X, ZHANG Z L, LI Z C, et al. Clinical study on modified Danggui Buxue Decoction against hepatitis and liver fibrosis[J]. Asia-Pacific Traditional Medicine, 2014, 10(23): 101-103. (in Chinese)
[10]	宫文霞, 周玉枝, 李肖, 等. 当归抗抑郁化学成分及药理作用研究进展[J]. 中草药, 2016, 47(21): 3905-3911.
	GONG W X, ZHOU Y Z, LI X, et al. Research progress in antidepressive active ingredients and pharmacological effects of Angelicae Sinensis Radix[J]. Chinese Traditional and Herbal Drugs, 2016, 47(21): 3905-3911. (in Chinese with English abstract)
[11]	廖铭能, 于立坚, 张永平, 等. 阿魏酸钠诱导分化的PC12细胞裂解液的无细胞滤液的抗抑郁样效果[J]. 中国细胞生物学学报, 2011, 33(6): 608-621.
	LIAO M N, YU L J, ZHANG Y P, et al. Antidepressant-like effect of cell-free filtrate of sodium ferulate-induced and differentioned PC12 cell lysates[J]. Chinese Journal of Cell Biology, 2011, 33(6): 608-621. (in Chinese with English abstract)
[12]	牛莉, 于泓苓. 中药当归的化学成分分析与药理作用研究[J]. 中西医结合心血管病电子杂志, 2018, 6(21): 90.
	NIU L, YU H L. Chemical constituents and pharmacological effects of Angelica sinensis[J]. Cardiovascular Disease Journal of Integrated Traditional Chinese and Western Medicine, 2018, 6(21): 90. (in Chinese)
[13]	毛宇, 徐芳, 邹云, 等. 当归多糖对造血功能的影响及其机制的研究[J]. 食品研究与开发, 2015, 36(8): 122-126.
	MAO Y, XU F, ZOU Y, et al. Research on the effect and mechanism of Angelica sinensis polysaccharide on hematopoietic function[J]. Food Research and Development, 2015, 36(8): 122-126. (in Chinese with English abstract)
[14]	张梦思, 邹婷, 叶渊文, 等. 当归多糖对衰老模型大鼠脾脏结构与功能的影响[J]. 解剖学报, 2015, 46(2): 257-264.
	ZHANG M S, ZOU T, YE Y W, et al. Effects of Angelica sinensis polysaccharide on the spleen structure and function of aging rats[J]. Acta Anatomica Sinica, 2015, 46(2): 257-264. (in Chinese with English abstract)
[15]	王瑞琼, 吴国泰, 任远, 等. 当归挥发油对兔离体胃肠平滑肌张力的影响[J]. 甘肃中医学院学报, 2010, 27(1): 12-14.
	WANG R Q, WU G T, REN Y, et al. Effects of angelica essential oil on the tension of extracorporeal gastrointestinal smooth muscle of rabbits[J]. Journal of Gansu College of Traditional Chinese Medicine, 2010, 27(1): 12-14. (in Chinese with English abstract)
[16]	ZHANG Y, LI Q A, FENG Y M, et al. Simultaneous determination of eight chemical components in Angelicae sinensis Radix and its herbal products by QAMS[J]. Journal of Analytical Methods in Chemistry, 2021, 2021: 1-7.
[17]	KAWABATA K, YAMAMOTO T, HARA A, et al. Modifying effects of ferulic acid on azoxymethane-induced colon carcinogenesis in F344 rats[J]. Cancer Letters, 2000, 157(1): 15-21.
[18]	TANAKA T, KOJIMA T, KAWAMORI T, et al. Inhibition of 4-nitroquinoline-1-oxide-induced rat tongue carcinogenesis by the naturally occurring plant phenolics caffeic, ellagic, chlorogenic and ferulic acids[J]. Carcinogenesis, 1993, 14(7): 1321-1325.
[19]	葛雅南, 顾婷, 刘俊岑, 等. 逍遥散中三种类植物雌激素对MCF-7细胞增殖的影响[J]. 黑龙江科技信息, 2016(5): 141-142.
	GE Y N, GU T, LIU J C, et al. Effects of three kinds of phytoestrogens in Xiaoyao Powder on the proliferation of MCF-7 cells[J]. Heilongjiang Science and Technology Information, 2016(5): 141-142. (in Chinese)
[20]	CHOI J H, PARK J K, KIM K M, et al. In vitro and in vivo antithrombotic and cytotoxicity effects of ferulic acid[J]. Journal of Biochemical and Molecular Toxicology, 2018, 32(1): e22004.
[21]	徐俊雄, 覃建兵, 王岩岩, 等. 虎杖MYB转录因子PcMYB1的表达特性和功能研究[J]. 中草药, 2020, 51(3): 726-732.
	XU J X, QIN J B, WANG Y Y, et al. Expression and function analysis of transcription factor PcMYB1 from Polygonum cuspidatum[J]. Chinese Traditional and Herbal Drugs, 2020, 51(3): 726-732. (in Chinese with English abstract)
[22]	段乐鹏, 谢丽琼, 马艺沔, 等. 转录因子对药用植物次生代谢的调控作用[J]. 中药材, 2021, 44(4): 1002-1007.
	DUAN L P, XIE L Q, MA Y M, et al. Regulation of transcription factors on secondary metabolism of medicinal plants[J]. Journal of Chinese Medicinal Materials, 2021, 44(4): 1002-1007. (in Chinese)
[23]	邱文怡, 王诗雨, 李晓芳, 等. MYB转录因子参与植物非生物胁迫响应与植物激素应答的研究进展[J]. 浙江农业学报, 2020, 32(7): 1317-1328.
	QIU W Y, WANG S Y, LI X F, et al. Functions of plant MYB transcription factors in response to abiotic stress and plant hormones[J]. Acta Agriculturae Zhejiangensis, 2020, 32(7): 1317-1328. (in Chinese with English abstract)
[24]	冯琛, 王玲, 汤浩茹, 等. 草莓MYB类转录因子FaMYB5基因的克隆与表达分析[J]. 浙江农业学报, 2016, 28(8): 1351-1359.
	FENG C, WANG L, TANG H R, et al. Cloning and expression analysis of MYB transcription factor FaMYB5 gene from strawberry[J]. Acta Agriculturae Zhejiangensis, 2016, 28(8): 1351-1359. (in Chinese with English abstract)
[25]	刘忠丽, 丛悦玺, 苟维超, 等. MYB类转录因子在植物细胞生长发育中的作用及其应用[J]. 浙江农业学报, 2012, 24(1): 174-179.
	LIU Z L, CONG Y X, GOU W C, et al. The role and application of MYB-type transcription factors in the plant growth and development[J]. Acta Agriculturae Zhejiangensis, 2012, 24(1): 174-179. (in Chinese with English abstract)
[26]	李君霞, 王春义, 丁宇涛, 等. MYB转录因子在植物耐盐基因工程中的应用进展[J]. 浙江农业学报, 2020, 32(10): 1910-1920.
	LI J X, WANG C Y, DING Y T, et al. Progress on application of MYB transcription factor in plant salt tolerance genetic engineering[J]. Acta Agriculturae Zhejiangensis, 2020, 32(10): 1910-1920. (in Chinese with English abstract)
[27]	杨迪, 张凯旋, 赵辉, 等. 苦荞转录因子FtNAC11的克隆及其功能分析[J]. 植物遗传资源学报, 2021, 22(5): 1430-1441.
	YANG D, ZHANG K X, ZHAO H, et al. Molecular cloning and functional analysis of FtNAC11 from Tartary buckwheat(Fagopyrum tataricum(L.) gaertn.)[J]. Journal of Plant Genetic Resources, 2021, 22(5): 1430-1441. (in Chinese with English abstract)
[28]	康珍, 杨迪, 郝彦蓉, 等. 苦荞转录因子FtMYB41的克隆及功能分析[J]. 植物遗传资源学报, 2022, 23(3): 895-905.
	KANG Z, YANG D, HAO Y R, et al. Molecular cloning and functional analysis of transcription factor FtMYB41 in Tartary buckwheat (Fagopyrum tataricum)[J]. Journal of Plant Genetic Resources, 2022, 23(3): 895-905. (in Chinese with English abstract)
[29]	牛义岭, 姜秀明, 许向阳. 植物转录因子MYB基因家族的研究进展[J]. 分子植物育种, 2016, 14(8): 2050-2059.
	NIU Y L, JIANG X M, XU X Y. Reaserch advances on transcription factor MYB gene family in plant[J]. Molecular Plant Breeding, 2016, 14(8): 2050-2059. (in Chinese with English abstract)
[30]	王霜, 雒晓鹏, 姚英俊, 等. 苦荞R2R3-MYB转录因子调控原花青素生物合成的研究[J]. 西北植物学报, 2019, 39(11): 1911-1918.
	WANG S, LUO X P, YAO Y J, et al. Characterization of an R2R3-MYB transcription factor involved in the synthesis of proanthocyanidins from Tartary buckwheat[J]. Acta Botanica Boreali-Occidentalia Sinica, 2019, 39(11): 1911-1918. (in Chinese with English abstract)
[31]	LUO X P, LI S J, YAO P F, et al. The jasmonate-ZIM domain protein FtJAZ2 interacts with the R2R3-MYB transcription factor FtMYB3 to affect anthocyanin biosynthesis in Tartary buckwheat[J]. Turkish Journal of Biology, 2017, 41: 526-534.
[32]	BAO X, ZONG Y, HU N, et al. Functional R2R3-MYB transcription factor NsMYB1, regulating anthocyanin biosynthesis, was relative to the fruit color differentiation in Nitraria sibirica Pall[J]. BMC Plant Biology, 2022, 22(1): 186-198.
[33]	陈江, 唐小慧, 任超翔, 等. 调控红花类黄酮合成MYB转录因子基因克隆及表达分析[J]. 中草药, 2017, 48(21): 4523-4529.
	CHEN J, TANG X H, REN C X, et al. Cloning and expression analysis of MYB transcription factor gene involved in regulation of flavonoids biosynthesis in Carthamus tinctorius[J]. Chinese Traditional and Herbal Drugs, 2017, 48(21): 4523-4529. (in Chinese with English abstract)
[34]	HORSCH R B, FRY J, HOFFMANN N, et al. Leaf disc transformation[M]// Plant Molecular Biology Manual. Dordrecht: Springer Netherlands, 1989: 63-71.
[35]	LI Y, CHEN M, WANG S L, et al. AtMYB11 regulates caffeoylquinic acid and flavonol synthesis in tomato and tobacco[J]. Plant Cell, Tissue and Organ Culture(PCTOC), 2015, 122(2): 309-319.
[36]	PANDEY A, MISRA P, TRIVEDI P K. Constitutive expression of Arabidopsis MYB transcription factor, AtMYB11, in tobacco modulates flavonoid biosynthesis in favor of flavonol accumulation[J]. Plant Cell Reports, 2015, 34(9): 1515-1528.
[37]	LUO J E, BUTELLI E, HILL L, et al. AtMYB12 regulates caffeoyl quinic acid and flavonol synthesis in tomato: expression in fruit results in very high levels of both types of polyphenol[J]. The Plant Journal, 2008, 56(2): 316-326.
[38]	LI L, WANG D H, ZHOU L, et al. JA-responsive transcription factor SmMYB97 promotes phenolic acid and tanshinone accumulation in Salvia miltiorrhiza[J]. Journal of Agricultural and Food Chemistry, 2020, 68(50): 14850-14862.
[39]	BAI Y C, LI C L, ZHANG J W, et al. Characterization of two Tartary buckwheat R2R3-MYB transcription factors and their regulation of proanthocyanidin biosynthesis[J]. Physiologia Plantarum, 2014, 152(3): 431-440.
[40]	ZHANG K X, LOGACHEVA M D, MENG Y, et al. Jasmonate-responsive MYB factors spatially repress rutin biosynthesis in Fagopyrum tataricum[J]. Journal of Experimental Botany, 2018, 69(8): 1955-1966.
[41]	伍小方, 高国应, 左倩, 等. FtMYB1转录因子调控苦荞毛状根黄酮醇合成的机理研究[J]. 植物遗传资源学报, 2020, 21(5): 1270-1278.
	WU X F, GAO G Y, ZUO Q, et al. Deciphering the functional basis of FtMYB1 transcription factor in flavonol biosynthesis of Tartary buckwheat hairy root[J]. Journal of Plant Genetic Resources, 2020, 21(5): 1270-1278. (in Chinese with English abstract)
[42]	PANDEY A, MISRA P, CHANDRASHEKAR K, et al. Development of AtMYB12-expressing transgenic tobacco callus culture for production of rutin with biopesticidal potential[J]. Plant Cell Reports, 2012, 31(10): 1867-1876.
[43]	PANDEY A, MISRA P, KHAN M P, et al. Co-expression of Arabidopsis transcription factor, AtMYB12, and soybean isoflavone synthase, GmIFS1, genes in tobacco leads to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity[J]. Plant Biotechnology Journal, 2014, 12(1): 69-80.
[44]	MISRA P, PANDEY A, TIWARI M, et al. Modulation of transcriptome and metabolome of tobacco by Arabidopsis transcription factor, AtMYB12, leads to insect resistance[J]. Plant Physiology, 2010, 152(4): 2258-2268.
[45]	DONG N Q, LIN H X. Contribution of phenylpropanoid metabolism to plant development and plant-environment interactions[J]. Journal of Integrative Plant Biology, 2021, 63(1): 180-209.
[46]	HAO G P, JIANG X Y, FENG L, et al. Cloning, molecular characterization and functional analysis of a putative R2R3-MYB transcription factor of the phenolic acid biosynthetic pathway in S. miltiorrhiza Bge. f. alba[J]. Plant Cell, Tissue and Organ Culture(PCTOC), 2016, 124(1): 151-168.
[47]	YANG R, WANG S S, ZOU H L, et al. R2R3-MYB transcription factor SmMYB52 positively regulates biosynthesis of salvianolic acid B and inhibits root growth in Salvia miltiorrhiza[J]. International Journal of Molecular Sciences, 2021, 22(17): 9538.
[48]	ZHOU W, SHI M, DENG C P, et al. The methyl jasmonate-responsive transcription factor SmMYB1 promotes phenolic acid biosynthesis in Salvia miltiorrhiza[J]. Horticulture Research, 2021, 8: 10.
[49]	ZHANG S C, MA P D, YANG D F, et al. Cloning and characterization of a putative R2R3 MYB transcriptional repressor of the rosmarinic acid biosynthetic pathway from Salvia miltiorrhiza[J]. PLoS One, 2013, 8(9): e73259.
[50]	LI S S, WU Y C, KUANG J, et al. SmMYB111 is a key factor to phenolic acid biosynthesis and interacts with both SmTTG1 and SmbHLH51 in Salvia miltiorrhiza[J]. Journal of Agricultural and Food Chemistry, 2018, 66(30): 8069-8078.
[51]	DING K, PEI T L, BAI Z Q, et al. SmMYB 36, a novel R2R3-MYB transcription factor, enhances tanshinone accumulation and decreases phenolic acid content in Salvia miltiorrhiza hairy roots[J]. Scientific Reports, 2017, 7: 5104.

Cloning and functional research of MYB transcription factor AsMYB44 from Angelica sinensis

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