Effects of exogenous spraying of amino acid fertilizer on growth and selenium uptake of watercress

doi:10.3969/j.issn.1004-1524.20221184

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (7): 1638-1647.DOI: 10.3969/j.issn.1004-1524.20221184

• Horticultural Science • Previous Articles Next Articles

Effects of exogenous spraying of amino acid fertilizer on growth and selenium uptake of watercress

XIAO Jiachang¹(), LEI Fengyun², GE Sang³, MA Junying¹, HE Maolin¹, LI Yanwen¹, ZHENG Yangxia¹^,^*()

1. College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China
2. Agricultural Equipment Research Institute, Chengdu Academy of Agriculture and Forestry Sciences, Chengdu 611130, China
3. Shannan Agricultural Technology Extension Center of Tibet Autonomous Region, Shannan 856000, Tibet, China

Received:2022-08-19 Online:2023-07-25 Published:2023-08-17
Contact: ZHENG Yangxia

Abstract

Abstract:

In order to improve the selenium absorption capacity of watercress (Nasturtium officinale R. Br.), pot experiments were conducted to investigate the effects of different concentrations of amino acid fertilizer (dilute 0, 600, 900, 1 200 and 1 500 times) on growth and selenium absorption of watercress under selenium treatment (5 mg·kg^-1). The results showed that exogenous application of amino acid fertilizer could increase the biomass of root and shoot of watercress, promote the content of chlorophyll and carotenoid in leaves, increase the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and reduce the content of soluble protein. The results of principal component analysis showed that 900 times dilution of amino acid fertilizer had the best growth promotion effect on watercress, and the root and shoot biomass increased significantly by 73.03% and 56.65% compared with the control, respectively. The application of 900-1 500 times dilution of amino acid fertilizer could also increase the total selenium accumulation in root, stem and leaf of watercress, but had no positive effect on the selenium content in root, stem, leaf and shoot of watercress. Correlation analysis showed that the biomass and total selenium accumulation of watercress were positively correlated with chlorophyll content, carotenoid content, POD activity, SOD activity, CAT activity and soil pH value. In conclusion, amino acid fertilizer can promote the growth of watercress and increase the total selenium accumulation in watercress.

Key words: amino acid fertilizer, watercress, biomass

CLC Number:

S645

XIAO Jiachang, LEI Fengyun, GE Sang, MA Junying, HE Maolin, LI Yanwen, ZHENG Yangxia. Effects of exogenous spraying of amino acid fertilizer on growth and selenium uptake of watercress[J]. Acta Agriculturae Zhejiangensis, 2023, 35(7): 1638-1647.

Figures/Tables 8

References 43

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氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	每株生物量Biomass per plant/g
氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	根Root	茎Stem	叶Leaf	地上部Shoot	根冠比Root/shoot ratio
0	0.152±0.004 d	0.260±0.011 d	0.545±0.010 c	0.805±0.021 d	0.189±0.008 b
600	0.158±0.003 cd	0.388±0.007 c	0.577±0.017 c	0.965±0.024 c	0.164±0.001 c
900	0.263±0.005 a	0.453±0.013 a	0.808±0.018 a	1.261±0.031 a	0.209±0.007 a
1 200	0.225±0.003 b	0.433±0.008 ab	0.750±0.011 b	1.183±0.020 b	0.190±0.005 b
1 500	0.165±0.004 c	0.420±0.006 b	0.740±0.016 b	1.160±0.021 b	0.142±0.005 d

氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	每株生物量Biomass per plant/g
氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	根Root	茎Stem	叶Leaf	地上部Shoot	根冠比Root/shoot ratio
0	0.152±0.004 d	0.260±0.011 d	0.545±0.010 c	0.805±0.021 d	0.189±0.008 b
600	0.158±0.003 cd	0.388±0.007 c	0.577±0.017 c	0.965±0.024 c	0.164±0.001 c
900	0.263±0.005 a	0.453±0.013 a	0.808±0.018 a	1.261±0.031 a	0.209±0.007 a
1 200	0.225±0.003 b	0.433±0.008 ab	0.750±0.011 b	1.183±0.020 b	0.190±0.005 b
1 500	0.165±0.004 c	0.420±0.006 b	0.740±0.016 b	1.160±0.021 b	0.142±0.005 d

氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	叶绿素a含量 Chlorophyll a content	叶绿素b含量 Chlorophyll b content	总叶绿素含量 Total chlorophyll content	类胡萝卜素含量 Carotenoid content
0	0.291±0.005 c	0.130±0.002 b	0.421±0.007 c	0.044±0.005 c
600	0.312±0.006 b	0.135±0.006 ab	0.447±0.011 bc	0.047±0.003 c
900	0.355±0.007 a	0.148±0.002 a	0.503±0.009 a	0.061±0.003 a
1 200	0.350±0.006 a	0.147±0.008 a	0.497±0.014 a	0.057±0.004 ab
1 500	0.329±0.009 b	0.146±0.006 a	0.475±0.015 ab	0.050±0.001 bc

氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	叶绿素a含量 Chlorophyll a content	叶绿素b含量 Chlorophyll b content	总叶绿素含量 Total chlorophyll content	类胡萝卜素含量 Carotenoid content
0	0.291±0.005 c	0.130±0.002 b	0.421±0.007 c	0.044±0.005 c
600	0.312±0.006 b	0.135±0.006 ab	0.447±0.011 bc	0.047±0.003 c
900	0.355±0.007 a	0.148±0.002 a	0.503±0.009 a	0.061±0.003 a
1 200	0.350±0.006 a	0.147±0.008 a	0.497±0.014 a	0.057±0.004 ab
1 500	0.329±0.009 b	0.146±0.006 a	0.475±0.015 ab	0.050±0.001 bc

氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	POD活性 POD activity/ (U·g^-1min^-1)	SOD活性 SOD activity/ (U·g^-1min^-1)	CAT活性 CAT activity/ (U·g^-1min^-1)	可溶性蛋白含量 Soluble protein content/ (mg·g^-1)
0	1 255±7.78 d	190.5±7.02 c	2.003±0.070 e	80.89±4.72 a
600	1 300±9.90 c	205.7±4.79 b	2.270±0.062 d	74.41±2.42 a
900	1 474±2.83 a	234.3±4.79 a	5.348±0.072 a	52.92±3.71 c
1 200	1 459±12.73 a	213.0±4.04 b	5.047±0.088 b	58.47±2.94 bc
1 500	1 378±16.97 b	207.6±2.34 b	2.843±0.087 c	62.30±2.38 b

Effects of exogenous spraying of amino acid fertilizer on growth and selenium uptake of watercress

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项目 Items	成分Component
项目 Items	F₁	F₂
根系生物量Root biomass	0.913	0.361
地上部分生物量Shoot biomass	0.968	-0.231
叶绿素a含量Chlorophyll a content	0.996	-0.057
叶绿素b含量Chlorophyll b content	0.940	-0.332
总叶绿素含量Total chlorophyll content	0.991	-0.123
类胡萝卜素含量Carotenoid content	0.980	0.165
POD活性POD activity	0.988	-0.050
SOD活性SOD activity	0.932	0.216
CAT活性CAT activity	0.943	0.203
可溶性蛋白含量Soluble protein content	-0.984	0.137
根系硒含量Se content in root	-0.635	0.770
地上部分硒含量Se content in shoot	-0.977	0.119
根系硒总积累量Se accumulation in root	0.651	0.733
地上部分硒总积累量	0.881	-0.474
Se accumulation in shoot
土壤有效硒含量Soil available selenium	-0.870	-0.415
土壤pH值Soil pH value	0.757	0.263
特征值Eigenvalue	13.18	2.06
贡献率Eontribution rate/%	82.35	12.87
累计贡献率Eumulative contribution rate/%	82.35	95.23

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氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	硒含量Selenium content
氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	根Root	茎Stem	叶Leaf	地上部Shoot
0	4.090±0.047 a	0.895±0.025 a	1.376±0.036 a	2.271±0.044 a
600	3.778±0.105 b	0.800±0.008 b	1.286±0.029 a	2.087±0.015 b
900	3.505±0.107 c	0.722±0.022 c	1.032±0.040 c	1.754±0.044 d
1 200	3.173±0.068 d	0.760±0.027 bc	1.124±0.034 bc	1.883±0.005 c
1 500	2.913±0.060 e	0.759±0.035 bc	1.163±0.055 b	1.922±0.014 c

氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	硒含量Selenium content
氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	根Root	茎Stem	叶Leaf	地上部Shoot
0	4.090±0.047 a	0.895±0.025 a	1.376±0.036 a	2.271±0.044 a
600	3.778±0.105 b	0.800±0.008 b	1.286±0.029 a	2.087±0.015 b
900	3.505±0.107 c	0.722±0.022 c	1.032±0.040 c	1.754±0.044 d
1 200	3.173±0.068 d	0.760±0.027 bc	1.124±0.034 bc	1.883±0.005 c
1 500	2.913±0.060 e	0.759±0.035 bc	1.163±0.055 b	1.922±0.014 c