Effects of pyraclostrobin on polysaccharide and flavone in Dendrobium officinale

doi:10.3969/j.issn.1004-1524.20240296

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (1): 115-125.DOI: 10.3969/j.issn.1004-1524.20240296

• Horticultural Science • Previous Articles Next Articles

Effects of pyraclostrobin on polysaccharide and flavone in Dendrobium officinale

HUANG Hao¹^,²(), TANG Tao², XU Zhenlan², ZHAO Xueping²^,^*()

1. College of Food and Health, Zhejiang A&F University, Hangzhou 311300, China
2. Key Laboratory for Pesticide Residue Detection, Ministry of Agriculture and Rural Affairs, Institute of Agri-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2024-03-28 Online:2025-01-25 Published:2025-02-14

Abstract

Abstract:

In this paper, the residue dynamics of pyraclostrobin in Dendrobium officinale and its effect on the content of polysaccharide and flavone were studied, and the potential mechanism of the effect of pyraclostrobin on the content of polysaccharide and flavone in Dendrobium officinale was discussed by metabolomics. The results showed that on the 7th day after the last application, the polysaccharide content of LC (recommended dose treatment) and HC (high dose treatment) groups decreased by 31% and 38% (P<0.01) compared with BC (blank control) group, and increased to no significant difference on the 7th to 15th day until the end of sampling. There was no significant difference in the content of flavone among the three groups from 0 to 15 days, while the content of flavone in LC and HC groups was significantly higher than that in BC group from 30 to 90 days, and the difference was the most significant on the 60th day, with an increase of 31% and 34%, respectively (P<0.01). On the 7th day after the last application, the contents of several metabolites in LC and HC groups were significantly higher than those in BC group, phenylalanine increased by 100% and 70% (P<0.05), tyrosine increased by 120% and 97% (P<0.05), and metabolic pathways such as phenylalanine, tyrosine and tryptophan synthesis and nitrogen metabolism were significantly up-regulated; On the 60th day, the concentrations of phloretin, luteolin and 7-O-methyleriodictyol in LC group were 66%, 18% and 89% lower than those in BC group (P<0.05), and the biosynthesis pathways of flavone and flavonols and phenylalanine were significantly down-regulated in LC group, while there was no differentially enriched pathway between HC group and BC group. According to the residue dynamics, the resistance of Dendrobium officinale to pyraclostrobin stress was improved by increasing the concentration of amino acids in LC and HC groups 7 days after the last application, and the increase of phenylalanine and other flavonoid precursors also promoted the synthesis of flavone. The stress response of Dendrobium officinale in LC group was gradually weakened after 60 days, while that in HC group maintained a higher stress response intensity in response to higher concentrations of pyraclostrobin residues. On the 7th day, LC and HC may promote the decomposition of polysaccharide into soluble sugars to regulate the osmotic pressure of plants, thus reducing the content of polysaccharide. The results of this study can provide a basis for the rational application of pyraclostrobin in the planting process of Dendrobium officinale.

Key words: Dendrobium officinale, pyraclostrobin, polysaccharide, flavone, metabolism

CLC Number:

S481.8

HUANG Hao, TANG Tao, XU Zhenlan, ZHAO Xueping. Effects of pyraclostrobin on polysaccharide and flavone in Dendrobium officinale[J]. Acta Agriculturae Zhejiangensis, 2025, 37(1): 115-125.

Figures/Tables 12

References 25

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添加浓度 Additive concentration/ (mg·L^-1)	平均添加回收率 Average recovery	相对标准偏差 RSD
0.001	117.6	10.1
0.01	108.1	5.7
0.1	106.2	4.7

添加浓度 Additive concentration/ (mg·L^-1)	平均添加回收率 Average recovery	相对标准偏差 RSD
0.001	117.6	10.1
0.01	108.1	5.7
0.1	106.2	4.7

采样时间 Sampling time/d	LC		HC
采样时间 Sampling time/d	残留量 Residual concentration/(mg·kg^-1)	消解率 Digestion rate/%	残留量 Residual concentration/(mg·kg^-1)	消解率 Digestion rate/%
0	5.07±0.88	—	10.05±1.56	—
7	3.83±0.72	24	7.74±1.16	23
15	3.64±0.56	28	7.02±0.88	30
30	3.44±0.45	32	6.24±0.94	38
60	2.53±0.39	50	5.27±0.8	48
90	1.87±0.37	63	4.05±0.64	60

采样时间 Sampling time/d	LC		HC
采样时间 Sampling time/d	残留量 Residual concentration/(mg·kg^-1)	消解率 Digestion rate/%	残留量 Residual concentration/(mg·kg^-1)	消解率 Digestion rate/%
0	5.07±0.88	—	10.05±1.56	—
7	3.83±0.72	24	7.74±1.16	23
15	3.64±0.56	28	7.02±0.88	30
30	3.44±0.45	32	6.24±0.94	38
60	2.53±0.39	50	5.27±0.8	48
90	1.87±0.37	63	4.05±0.64	60

编号No.	化合物Compound	VIP	Fold change	P-value
1	R-芳樟基β-葡萄糖苷R-cinnamyl β-glucoside	3.30	3.69	3.65×10^-2
2	9-氧代十八碳-1012-二烯酸9-oxooctadecane-1012-dienoic acid	3.05	4.10	4.83×10^-2
3	谷氨酰胺Glutamine	2.84	3.84	2.04×10^-2
4	L-谷胱甘肽(氧化形式)L-Glutathione (oxidized form)	2.69	3.05	3.20×10^-2
5	酪氨酸Tyrosine	2.35	2.20	4.07×10^-2
6	色氨酸Tryptophan	2.01	1.65	2.69×10^-2
7	苯丙氨酸Phenylalanine	1.91	2.00	3.59×10^-2
8	天冬酰胺Asparagine	1.82	7.55	2.36×10^-2
9	L-异亮氨酸L-isoleucine	1.70	3.42	2.53×10^-2
10	苯甲醛Benzaldehyde	1.68	0.76	7.61×10^-3
11	(6β,22E)-6-羟基豆甾-4,22-二烯-3-酮(6 β, 22E)-6-Hydroxystigmastan-4,22-dien-3-one	1.36	2.25	3.90×10^-2
12	尿苷Uridine	1.33	2.61	3.83×10^-3
13	DL-异亮氨酸DL-isoleucine	1.28	5.74	2.02×10^-3
14	组氨酸Histidine	1.27	2.82	9.79×10^-3
15	L-焦谷氨酸L-pyroglutamic acid	1.26	2.66	4.45×10^-2
16	N-环己基甲酰胺N-cyclohexylformamide	1.26	0.79	3.88×10^-2
17	哌啶Piperidine	1.21	5.24	2.38×10^-3
18	叶黄Xanthophyll	1.18	2.74	2.23×10^-2
19	谷氨酰胺类似物,1 Glutamine analogues, 1	1.15	4.06	8.78×10^-3

Effects of pyraclostrobin on polysaccharide and flavone in Dendrobium officinale

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编号No.	化合物Compound	VIP	Fold change	P-value
1	蔗糖Sucrose	5.89	2.68	4.03×10^-2
2	Hydron;2-hydroxypropane-1,2,3-tricarboxylate	2.69	2.49	1.79×10^-2
3	谷氨酰胺Glutamine	2.65	3.23	1.16×10^-2
4	(4-羟基-3,5-二甲氧基苯基)甲基-β-D-吡喃葡萄糖苷	2.41	2.25	4.88×10^-2
	(4-hydroxy-3,5-dimethoxyphenyl) methyl-β-D-glucopyranoside
5	酪氨酸Tyrosine	2.29	1.97	2.4×10^-3
6	色氨酸Tryptophan	1.98	1.77	3.94×10^-2
7	R-芳樟基β-葡萄糖苷R-cinnamyl β-glucoside	1.64	3.63	3.32×10^-2
8	苯丙氨酸Phenylalanine	1.33	1.70	3.27×10^-2
9	叶黄Xanthophyll	1.21	2.33	3.84×10^-2
10	2-糠酸2-Furoic acid	1.2	2.1	1.82×10^-2
11	葡萄糖Glucose	1.13	1.71	3.87×10^-2
12	91213-Trihydroxyoctadeca-1015-dienoic acid	1.12	3.53	4.3×10^-3
13	焦谷氨酸Pyroglutamic acid	1.12	2.49	1.06×10^-2

编号No.	化合物Compound	VIP	Fold change	P-value
1	根皮素Phloretin	2.99	0.34	3.26×10^-2
2	芥子碱Sinapine	2.61	0.18	1.42×10^-2
3	莽草酸Shikimic acid	2.52	0.22	5.94×10^-3
4	2',4',6'-三羟基二氢查耳酮2', 4', 6'-trihydroxydihydrochalcone	1.61	0.25	4.77×10^-2
5	2'-羟基-4,4',6'-三甲氧基查耳酮2'-hydroxy-4,4', 6'-trimethoxychalcone	1.49	0.47	5.8×10^-3
6	异丹叶大黄素Isorhapontigenin	1.42	0.23	4.88×10^-3
7	木犀草Luteolin	1.41	0.82	2.27×10^-2
8	NΩ-单甲基-L-精氨酸乙酸盐NΩ-Monomethyl-L-arginine Acetate	1.38	0.19	4.30×10^-2
9	3-(2,4-二羟基苯基)-7-羟基-6,8-双(3-甲基丁-2-烯基)-2,3-二氢罗马烯-4-酮	1.35	4.19	1.87×10^-3
	3-(2,4-dihydroxyphenyl)-7-hydroxy-6,8-bis (3-methylbut-2-enyl)-2,3-dihydroRomane-4-one
10	茶碱Theophylline	1.31	0.22	3.57×10^-2
11	7-O-甲基圣草酚7-O-methylcatechol	1.26	0.11	1.53×10^-2
12	2-巯基苯并噻唑2-Mercaptobenzothiazole	1.15	1.62	4.63×10^-2

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