Polymorphism analysis of Colletotrichum spp. from Zhejiang Province by ITS rDNA PCRRFLP

›› 2012, Vol. 24 ›› Issue (1): 0-65.

• 植物保护 •

Polymorphism analysis of Colletotrichum spp. from Zhejiang Province by ITS rDNA PCRRFLP

LIU Shiyin;QIU Haiping;JIANG Hua;ZHANG Zhen;CHAI Rongyao;MAO Xueqin;WANG Jiaoyu;DU Xinfa;WANG Yanli;*;SUN Guochang;*

1State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control，Institute of Plant Protection and Microbiology，Zhejiang Academy of Agricultural Sciences， Hangzhou 310021，China；2College of Life and Environment Sciences，Hangzhou Normal University，Hangzhou 310036，China

Received:1900-01-01 Revised:1900-01-01 Online:2012-01-24 Published:2012-01-24

Abstract

Abstract: Colletotrichum spp. is the most common fungi in Zhejiang Province as well as the most important plant pathogens. There are so many kinds of species that it is important to find a way to identify and classify these fungi. Genetic diversity among 32 strains belonging to Colletotrichum spp. was assessed by RFLP analysis on the basis of rDNA ITS region. The PCR amplified ITS region was about 600 bp in length in all the tested strains and RFLP patterns of ITS products digested with different endonucleases (AluⅠ， BsuRⅠ， Hha Ⅰ， HpaⅡ and TaqⅠ) were analyzed. The UPGMA phenogram constructed with the pool of the ITS PCRRFLP data revealed that all the isolates were clustered on one big group when the value of genetic similarity was 0.6. The Gst parameter of all the ITS PCRRFLP polymorphic bands was 0.82 which indicated there were high diversity in the ITS region of all the isolates．

Key words: Colletotrichum spp., rDNA, ITS, PCRRFLP, genetic relationships

LIU Shiyin;QIU Haiping;JIANG Hua;ZHANG Zhen;CHAI Rongyao;MAO Xueqin;WANG Jiaoyu;DU Xinfa;WANG Yanli;*;SUN Guochang;*. Polymorphism analysis of Colletotrichum spp. from Zhejiang Province by ITS rDNA PCRRFLP [J]. , 2012, 24(1): 0-65.

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Polymorphism analysis of Colletotrichum spp. from Zhejiang Province by ITS rDNA PCRRFLP

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