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Detecting the rice blast resistance genes and brown planthopper resistance genes by gene functional marker

  

  1. (1 College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China; 2 Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China)
  • Online:2015-03-25 Published:2015-03-31

Abstract: Breeding and utilizing resistant rice cultivars is an effective method for controlling rice blast and brown planthopper. The rice blast resistance genes Pi\|b, Pi\|k, Pi\|ta and brown planthopper resistance genes bph2, Bph3 of 2 male sterile lines and their 16 restorer lines were detected by gene functional marker. The results showed that 11 tested materials contained gene Pi\|b, 13 tested materials contained gene Pi\|k, 9 tested materials contained both gene Pi\|b and Pi\|k, and 3 tested materials contained gene bph2. It laid the foundation for the gene pyramiding and breeding the excellent rice cultivars with broad spectrum and durable resistance to rice blast and brown planthopper by molecular identification to related resistance genes of rice cultivars.

Key words: rice blast, rice brown planthopper, resistance gene, functional markers, gene pyramiding