Previous Articles     Next Articles

Change of phosphatases activity in different tissues of grass carp(Ctenopharyngodon idella) after exposing to Hg2+#br#

  

  1. (1 Institute of Biotechnology, Jiaozuo Teachers College, Jiaozuo 454001, China; 2 College of Life Science, Henan Normal University, Xinxiang 453007, China)
  • Online:2015-09-25 Published:2015-09-23

Abstract:  In this study, the experimental grass carps were placed in Hg2+ (05mg·L-1) for 24 h first and then in cultivated water. Afterwards, they were sampled at different recovering times (1, 5, 12, 21 d) to test the activity changes of the alkaline phosphates (AKP) and acid phosphates (ACP) in their plasma, gill, hepatopancreas, spleen and kidney. The results were as follows: compared with the control group, the AKP activity in plasma(1 d), gill(1, 5, 12, 21 d), hepatopancreas(21 d) and spleen(1 d) decreased significantly (P<005,P<001) and increased remarkably in hepatopancreas(12 d) and kidney(1 d)(P<005, P<001), while there was no significant differences in plasma(5, 12, 21 d) , hepatopancreas(1,5 d), spleen(5, 12, 21 d) and kidney(5, 12, 21 d)(P>005). The ACP activity in gill(1, 5, 12, 21 d)and hepatopancreas(12, 21 d), spleen (1,5 d) dropped remarkably (P<005, P<001)and rose remarkably in plasma(1, 5 d), hepatopancreas(1, 5 d), spleen (1,5 d) and kidney(1 d) (P<005, P<001 ) while there were no obvious changes in plasma(12, 21 d), spleen(12,21 d) and kidney(5, 12, 21 d)(P>005). This study indicated that the gill and hepatopancreas were more vulnerable to Hg2+ than plasma, spleen and kidney, and their AKP and ACP actirities were not restored in the trial period.

Key words: Hg2+, alkaline phosphates, acid phosphates, tissue, organ, Ctenopharyngodon idella