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Identification and analysis of GPX genes in Chinese cabbage

  

  1. (1. Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China)
  • Online:2016-01-25 Published:2016-01-19

Abstract:  To study the biological characteristics of glutathione peroxidase (GPX) genes in cabbage, 10 GPX genes were screened from the whole genome database of cabbage. Then expression levels of these GPX genes were analyzed based on cabbage transcriptome RNA\|Seq data at embryonic development and in different organs. Meantime, gene mapping, promoter, gene structure, phylogenetic tree and expression level of these 10 GPX genes were studied using bioinformatics tools. The results showed that there were six introns in the coding region of BrGPX5, while other genes only had five introns, which indicated that BrGPX5 gene obtained an intron during evolution, resulting in increased molecular weight of the amino acid. In addition, there were two and above E-box elements in the promoter region of each GPX gene in Chinese cabbage.

Key words: Chinese cabbage, GPX genes, promoter, gene expression