Abstract: The Partridge chickens whose eggs were quinoiones （QNs） negative tested by the immune colloidal gold rapid detection were used as the experimental materials. The chickens were injected twice each day for two consecutive days with 5 mg·kg-1 ciprofloxacin， 5 mg·kg-1 enrofloxacin and 10 mg·kg-1 norfloxacin， respectively. LCMS/MS analysis was performed to detect the residues of twelve quinoiones in muscle， liver， blood and follicles. The results showed that each drug could be converted into six or more QNs drugs in chicken. Moreover， the residual amount of QNs varied in different tissues. The QNs residues in muscle and blood were lower and had faster metabolic rate （the residues were less than 19 μg·kg-1 at the 9th withdrawal day） than those of other tissues， while the residual amount of QNs were higher in liver and follicles. The ciprofloxacin， norfloxacin and their transformation products were enriched in liver， and the norfloxacin metabolism rate in liver was slow （the residue of QNs was 1 7737 μg·kg-1 at the 9th withdrawal day）. The enrofloxacin in chickens was rapidly converted into other QNs， which was highly enriched in follicles. The conversion rate of enrofloxacin was up to 100% in liver， blood and follicles at the 48th withdrawal hour after injection with enrofloxacin. The residues of QNs in follicles was up to 8 3273 μg·kg-1. Therefore， the use of enrofloxacin in layers might result in large amounts of QNs residues in eggs.

Key words: quinolones, transformation, Partridge chicken, LCMS/MS, residue