Prevalence and molecular identification of Cryptosporidium spp. in yaks in Hongyuan County of Sichuan Province

doi:10.3969/j.issn.1004-1524.2016.11.06

›› 2016, Vol. 28 ›› Issue (11): 1842-1846.DOI: 10.3969/j.issn.1004-1524.2016.11.06

• Animal Science • Previous Articles Next Articles

Prevalence and molecular identification of Cryptosporidium spp. in yaks in Hongyuan County of Sichuan Province

HAO Li-li¹, LI Rui², DUAN Ling¹, HE Liang^{3, *}

1. College of Life Science & Technology, Southwest University for Nationalities, Chengdu 610041, China;
2. Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. National Shanghai Center for New Drug Safety Evaluation and Research, Shanghai 201203, China

Received:2016-03-04 Online:2016-11-15 Published:2016-11-16

Abstract

Abstract: In order to investigate prevalence of Cryptosporidium spp. in yaks in Hongyuan County, in this study, Cryptosporidium isolates from 216 fecal samples were identified by nest-PCR method (including 18 diarrhea fecal samples). Total infection rate of Cryptosporidium was 14.8%. It showed that prevalence of Cryptosporidium bovis, C. ryanae and C. andersoni was 3.7%, 5.1% and 6.0%, respectively. However, no Cryptosporidium spp. was detected in 18 diarrhea fecal samples. Above results showed that yaks were natural hosts of Cryptosporidium spp.. But in this study, no obvious correlation existed between Cryptosporidium spp. and diarrhea, and the relationship between Cryptosporidium spp. and diarrhea in yaks should be further explored.

Key words: yaks, Cryptosporidium spp., diarrhea

CLC Number:

S855.9⁺9

HAO Li-li, LI Rui, DUAN Ling, HE Liang. Prevalence and molecular identification of Cryptosporidium spp. in yaks in Hongyuan County of Sichuan Province[J]. , 2016, 28(11): 1842-1846.

References

[1] LINDSAY D S, UPTON S J, OWENS D S, et al. Cryptosporidium andersoni , n. sp. (Apicomplexa: Cryptosporiidae) from cattle, Bos taurus [J]. Journal of Eukaryotic Microbiology , 2000, 47(1): 91-95.
[2] 彭昊,李军,陶立,等.安氏隐孢子虫ITS-1基因PCR检测方法的建立[J].南方农业学报, 2012,43(5):701-704.
PENG H, LI J, TAO L, et al. Establishment of PCR assay for detection of ITS-1 gene in Cryptosporidium andersoni [J]. Journal of Southern Agriculture , 2012, 43(5):701-704.(in Chinese with English abstract)
[3] XIAO L H, FAYER R, RYAN U, et al. Cryptosporidium taxonomy: recent advances and implications for public health[J]. Clinical Microbiology Reviews , 2004, 17(1):72-97.
[4] 张龙现,蒋金书.隐孢子虫和隐孢子虫病研究进展[J].寄生虫与医学昆虫学报,2001,8(3):184-191.
ZHANG L X, JIANG J S. Research progression of Cryptosporidium and cryptosporidiosis[J]. Acta Parasitologica et Medica Entomologica Sinica , 2001,8(3):184-191.(in Chinese with English abstract)
[5] AZAMI M , MOGHADDAM D D, SALEHI R, et al. The identification of Cryptosporidium species (protozoa) in Ifsahan, Iran by PCR-RFLP analysis of the 18S rRNA gene[J]. Molekuliarnaia Biologiia , 2007, 41(5):934-939.
[6] 曾铁兵, 吴移谋, 黄澍杰, 等. 巢式PCR扩增梅毒螺旋体polA及其临床应用的研究[J]. 中国皮肤性病学杂志, 2004, 18(2):74-76.
ZENG T B, WU Y M, HUANG S J, et al. Amplification of the DNA polymerase Ⅰ gene of Treponema pallidum and its clinical application[J]. The Chinese Journal of Dermatovenereology , 2004, 18(2):74-76.(in Chinese with English abstract)
[7] 何美琳,张焕容,王永,等. 川西北牦牛3种病毒性腹泻病血清学调查[J]. 中国畜牧兽医, 2014, 41(3):248-251.
HE M L, ZHANG H R, WANG Y, et al. Serological survey on three viral diarrhea diseases of yaks in northwest Sichuan province[J]. China Animal Husbandry & Veterinary Medicine , 2014, 41(3):248-251.(in Chinese)
[8] LI P, CAI J Z, CAI M, et al. Distribution of Cryptosporidium species in Tibetan sheep and yaks in Qinghai, China[J]. Veterinary Parasitology , 2016, 215:58-62.
[9] MA J B, CAI J Z, MA J W, et al. Occurrence and molecular characterization of Cryptosporidium spp. in yaks (Bos grunniens) in China[J]. Veterinary Parasitology , 2014, 202(3/4):113-118.
[10] MI R S, WANG X J, LI C H, et al. Prevalence and genetic characterization of Cryptosporidium in yaks in Qinghai province of China[J]. PLoS One , 2013, 8(9):e74985.
[11] QI M, CAI J Z, WANG R J, et al. Molecular characterization of Cryptosporidium spp. and Giardia duodenalis from yaks in the central western region of China[J]. BMC Microbiology , 2015, 15(1):1-7.
[12] QIN S Y, ZHANG X X,ZHAO G H, et al. First report of Cryptosporidium spp. in white yaks in China[J]. Parasites & Vectors , 2014, 7(1):1-3.

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Prevalence and molecular identification of Cryptosporidium spp. in yaks in Hongyuan County of Sichuan Province

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