Effect of NDGA treatment on ABA content and expression of related genes in strawberry

doi:10.3969/j.issn.1004-1524.2016.12.13

›› 2016, Vol. 28 ›› Issue (12): 2047-2053.DOI: 10.3969/j.issn.1004-1524.2016.12.13

• Horticultural Science • Previous Articles Next Articles

Effect of NDGA treatment on ABA content and expression of related genes in strawberry

SONG Xiao-juan, LI Ya-li, XIAO Jie, LI Xin, LI Rui-ling, YE Yu-yun, TANG Hao-ru^*

College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China

Received:2016-04-14 Online:2016-12-15 Published:2017-01-05

Abstract

Abstract: The Benihoppe strawberry was treated with different concentrations of nordihydroguaiaretic acid (NDGA), which can depress the synthesis of abscisic acid (ABA) to investigate its influence on physiological indexes and related gene expression. The results showed that NDGA could significantly inhibit biosynthesis of soluble sugar and pelargonidin 3-O-glucoside chloride, and the inhibition became more obvious with the increasing concentration. In addition, FaNCED was inhibited and FaCYP707A, FaBG1 and FaCHLH were accelerated with a certain concentration of NDGA. Our study may provide helpful information for further study on molecular mechanism of NDGA on inhibiting the biosynthesis of ABA.

Key words: NDGA, ABA, strawberry, gene expression

CLC Number:

S668.4

SONG Xiao-juan, LI Ya-li, XIAO Jie, LI Xin, LI Rui-ling, YE Yu-yun, TANG Hao-ru. Effect of NDGA treatment on ABA content and expression of related genes in strawberry[J]. , 2016, 28(12): 2047-2053.

References

[1] 董敬超. ‘红颜’草莓茎尖组织培养快繁技术研究[J]. 北方园艺, 2013(24): 106-108.
DONG J C. Tissue culture of stem tip and rapid propagation of Fragaria×ananassa Duch ‘Benihopp e ’[J]. Northern Horticulture , 2013(24): 106-108. (in Chinese with English abstract)
[2] 朱海生,李永平,花秀凤,等. 草莓 9-顺式-环氧类胡萝卜素双加氧酶基因 FaNCED 的克隆及表达分析[J]. 园艺学报, 2012, 39(1): 40-48.
ZHU H S, LI Y P, HUA X F, et al. Cloning and expression analysis of 9-cis-epoxycarotenoid dioxygenase gene FaNCED in strawberry[J]. Acta Horticulturae Sinica , 2012, 39(1): 40-48. (in Chinese with English abstract)
[3] 贾海锋,柴叶茂,李春丽,等. 草莓果实中脱落酸受体基因 FaABAR/CHLH 表达变化及其影响因素分析[J]. 园艺学报, 2011, 38(9): 1650-1656.
JIA H F, CHAI Y M, LI C L, et al. Expression changes of ABA receptor gene FaABAR/CHLH in strawberry and its affection factors[J]. Acta Horticulturae Sinica , 2011, 38(9): 1650-1656. (in Chinese with English abstract)
[4] QIN X, ZEEVAART J A. Overexpression of a 9-cis-epoxycarotenoid dioxygenase gene in Nicotiana plumbaginifolia increases abscisic acid and phaseic acid levels and enhances drought tolerance[J]. Plant Physiology , 2002, 128(2): 544-551.
[5] LEE K H, Piao H L, Kim H Y, et al. Activation of glucosidase via stress-induced polymerization rapidly increases active pools of abscisic acid[J]. Cell , 2006, 126(6): 1109-1120.
[6] SAITO S, HIRAI N, MATSUMOTO C, et al. Arabidopsis CYP707As encode (+)-abscisic acid 8'-hydroxylase,a key enzyme in the oxidative catabolism of abscisic acid[J]. Plant Physiology , 2004, 134(4): 1439-1449.
[7] WU F Q, XIN Q, CAO Z, et al. The magnesium-chelatase H subunit binds abscisic acid and functions in abscisic acid signaling: new evidence in Arabidopsis [J]. Plant Physiology , 2009, 150(4): 1940-1954.
[8] 魏开发,陈娟,陈艳峰,等. 内源 ABA 信号水平动态调控的分子机制[J]. 遗传, 2012, 34(3): 296-306.
WEI K F, CHEN J, CHEN Y F, et al. Molecular mechanism for dynamic regulation of endogenous ABA signal level[J]. Hereditas , 2012, 34(3): 296-306. (in Chinese with English abstract)
[9] 迟晓雪,孙洁峰,方程. 结缕草种子中 NCED 基因和 CYP707A 基因的克隆及其表达动态[J]. 草地学报, 2012, 20(1):146-151, 165.
CHI X X, SUN J F, FANG C. Cloning and expression dynamics of NCED and CYP707A gene from Zoysia Japonica seeds[J]. Acta Agrestia Sinica , 2012, 20(1): 146-151, 165. (in Chinese with English abstract)
[10] 朱攀攀,刘长英,赵爱春,等. 桑树脱落酸生物合成相关基因的鉴定及转录表达分析[J]. 中国农业科学, 2015, 48(5): 1011-1022.
ZHU P P, LIU C Y, ZHAO A C, et al. Characterization and transcriptional expression analysis of ABA biosynthesis related genes from mulberry ( Morus alba L.) [J]. Scientia Agricultura Sinica , 2015,48(5): 1011-1022. (in Chinese with English abstract)
[11] 任杰,吴洁芳,冷平,等. 甜樱桃果实 NCED 基因的克隆及其表达[J]. 园艺学报, 2010, 37(6): 891-898.
REN J, WU J F, LENG P, et al. Cloning and expression analysis of NCED gene from sweet cherry fruit[J]. Acta Horticulturae Sinica , 2010, 37(6): 891-898. (in Chinese with English abstract)
[12] LOHANI S, TRIVEDI P K, NATH P. Changes in activities of cell wall hydrolases during ethylene-induced ripening in banana:effect of 1-MCP, ABA and IAA[J]. Postharvest Biology and Technology , 2004, 31(2): 119-126.
[13] CHEN J Y, CHEN M, GAN L. Effects of ethephon and ABA treatments on physiology of 'Jinkui' kiwifruit during its ripening and softening[J]. Acta Agriculturae Universitatis Jiangxiensis , 2004, 27(1): 6-11.
[14] ZHANG M, YUAN B, LENG P. The role of ABA in triggering ethylene biosynthesis and ripening of tomato fruit[J]. Journal of Experimental Botany , 2009, 60(6): 1579-1588.
[15] 熊庆娥. 植物生理学实验教程[M].成都: 四川科学技术出版社, 2003: 81-83.
[16] 于鲁浩. 总糖和柠檬酸的快速测定及在柠檬酸发酵过程中的应用[D]. 济南: 山东轻工业学院, 2012.
YU L H. Fast determination of total sugar and citric acid is build and used in the femantation of citric acid[D]. Jinan: Shandong Institute of Light Industry, 2012. (in Chinese with English abstract)
[17] 张娟娟. ‘丰香’草莓果实发育过程中主要品质特征成分的代谢谱分析[D]. 合肥: 安徽农业大学, 2009.
ZHANG J J. Metabolic profiling of the major fruit quality components during the development of Fragaria× ananassa 'Fengxiang'' fruits[D]. Hefei: Anhui Agricultural University, 2009. (in Chinese with English abstract)
[18] 王博. 根域限制促进鲜食葡萄果皮花色苷合成的机制研究 [D]. 上海: 上海交通大学, 2013.
WANG B. Study on the mechanism of improved anthocyanin biosynthesis under root restriction in table grapeberry skin[D]. Shanghai: Shanghai Jiao Tong University, 2013. (in Chinese with English abstract)
[19] CHEN Q, YU H W, WANG X R, et al. An alternative cetyltrimethylammonium bromide-based protocol for RNA isolation from blackberry ( Rubus L.)[J]. Genetics and Molecular Research : GMR , 2011, 11(2): 1773-1782.
[20] LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method[J]. Methods , 2001, 25(4): 402-408.
[21] 刘士华,吕国胜. 草莓果实成熟过程中糖,酸含量的动态变化[J]. 安徽林业科技, 2011, 37(5): 24-27.
LIU S H, LV G S. Study on dynamic changes of carbohydrates and organic acids in the strawberry fruit during its development[J]. Anhui Forest Technology , 2011, 37(5): 24-27. (in Chinese with English abstract)
[22] 刘雨佳,彭丽桃,叶俊丽,等. '法兰地' 草莓果实中花色素苷的组成及稳定性[J]. 华中农业大学学报, 2016, 35(1): 24-30.
LIU Y J, PENG L T, YE J L, et al. Anthocyanidin components and stability in Fragaria× ananassa 'Flange' fruits[J]. Journal of Huazhong Agricultural University , 2016, 35(1): 24-30. (in Chinese with English abstract)
[23] 李春丽,柴叶茂,王志忠,等. 草莓果实发育过程中糖,pH 值及 ABA 水平变化趋势[J]. 果树学报, 2011, 28(1): 72-76.
LI C L, CHAI Y M, WANG Z Z, et al. Changing trends of sugar, pH and ABA during strawberry fruit development[J]. Journal of Fruit Science , 2011, 28(1): 72-76. (in Chinese with English abstract)
[24] 刘廷旭. ABA 对桃果实成熟软化和乙烯释放信号转导组分的影响[D]. 杨凌: 西北农林科技大学, 2012.
LIU T X, The Impact of ABA on fruit ripening and the components of ethylene production and signal transduction[D]. Yangling: Northwest A&F University, 2012. (in Chinese with English abstract)
[25] 陈尚武,张大鹏. ABA 和 Fluridone 对苹果果实成熟的影响[J]. 植物生理学报, 2000, 26(2): 123-129.
CHEN S W, ZHANG D P. Effects of abscisic acid and fluridone on ripening of apple fruit[J]. Acta Phytophysiologica Sinica , 2000, 26(2): 123-129. (in Chinese with English abstract)
[26] NAMBARA E, MARION-POLL A. Abscisic acid biosynthesis and catabolism[J]. Annual Review of Plant Biology , 2005, 56: 165-185.
[27] DIETZ K J, SAUTER A, WICHERT K, et al. Extracellular β‐glucosidase activity in barley involved in the hydrolysis of ABA glucose conjugate in leaves[J]. Journal of Experimental Botany , 2000, 51(346): 937-944.
[28] 柴叶茂,贾海锋,李春丽,等. 草莓果实 ABA 积累关键酶基因 FaNCED1 和 FaBG1 转录水平的分析[C] //中国园艺学会. 中国园艺学会2010年学术年会论文摘要集. 2010: 1-4.
[29] 李倩. β-葡萄糖苷酶基因在调控果实成熟及内源 ABA 水平中的作用分析[D]. 北京: 中国农业大学, 2015.
LI Q. The role of 3-glucosidase genes in regulating fruit ripeningand endogenous ABA level[D]. Beijing: China Agricultural University, 2015. (in Chinese with English abstract)
[30] 祝军,柴璐,侯柄竹,等. 果实中脱落酸的研究进展与展望[J]. 园艺学报, 2015, 42(9): 1664-1672. (in Chinese with English abstract)
ZHU J, CHAI L, HOU B Z, et al. Research advances and prospects of ABA in fleshy fruit[J]. Acta Horticulturae Sinica , 2015, 42(9): 1664-1672. (in Chinese with English abstract)
[31] JIA H F, CHAI Y M, LI C L, et al. Abscisic acid plays an important role in the regulation of strawberry fruit ripening[J]. Plant Physiology , 2011, 157(1): 188-199.
[32] CHAI Y M, JIA H F, LI C L, et al. FaPYR1 is involved in strawberry fruit ripening[J]. Journal of Experimental Botany , 2011, 62(14): 5079-5089.

Effect of NDGA treatment on ABA content and expression of related genes in strawberry

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