Changes of GH, INS, HC, TGF-β1 and IGF-1 levels in cows’ serum during milk withdrawal period

doi:10.3969/j.issn.1004-1524.2017.04.05

›› 2017, Vol. 29 ›› Issue (4): 548-554.DOI: 10.3969/j.issn.1004-1524.2017.04.05

• Animal Science • Previous Articles Next Articles

Changes of GH, INS, HC, TGF-β1 and IGF-1 levels in cows’ serum during milk withdrawal period

SHEN Liuhong¹, WU Xiaofeng¹, XIAO Jinbang¹, JIANG Sixun¹, DENG Junliang¹, ZUO Zhicai¹, FU Hongqing², CAO Suizhong¹, YU Shumin^{1, *}, ZHANG Yourui³

1. The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease,College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China;
3. Agricultural Bureau of Langfang City, Langfang 065000, China

Received:2016-11-01 Online:2017-04-20 Published:2017-04-27

Abstract

Abstract: In order to explore the changes and correlation among the levels of growth hormone (GH), insulin (INS), hydrocortisone (HC), TGF-β1 and IGF-1 in cows’ serum during the period of milk withdrawal. Twenty healthy Chinese Holstein cows in late pregnancy that gave (15.43±2.10) kg milk per day were used, which were ready to dry milk. The day when cows began to dry milk was recorded as the 0 d. The cows’ venous blood from the tail on the day 0, 1, 3, 5, 7, 9 and 11 were collected. ELISA was used to evaluate the levels of GH, INS, HC, TGF-β1 and IGF-1 in serum to analyze the changes and correlation among them. The results showed that, during the period of milk withdrawal, the changes of GH, INS, HC and TGF-β1 levels in serum were not obvious from the day 0 to the day 1 (P>0.05). The levels of GH and HC in serum were both on the decline and had significant difference from the day 3 to the day 11 (P<0.01), and the levels of GH in serum on the day 3 was significantly higher than those on the other days (P<0.01). The levels of INS in serum was on the rise and had significant (P<0.01) difference from the day 3 to the day 11, while the levels of TGF-β1 in serum had no significant (P>0.05) difference from the day 3 to the day 11. The levels of IGF-1 in serum showed downward trend in ladder type, and the difference among levels of IGF-1 in serum on the day 0 and day 1, day 3 and day 5, day 7 and day 9, day 9 and day 11 were not obvious (P>0.05), while IGF-1 levels among 0-1 d, 3-5 d and 7-11 d had significant (P<0.01) difference. There was significant (P<0.01) correlation among the levels of GH, INS, TGF-β1 and IGF-1 in cows’ serum separately during the period of milk withdrawal. The HC levels showed significant (P<0.01) correlation with GH, INS and IGF-1 levels, while it showed significant (P<0.05) negative correlation with TGF-β1 levels.

Key words: cows in milk withdrawal period, GH, INS, HC, TGF-β, 1, IGF-1

CLC Number:

S857.2

SHEN Liuhong, WU Xiaofeng, XIAO Jinbang, JIANG Sixun, DENG Junliang, ZUO Zhicai, FU Hongqing, CAO Suizhong, YU Shumin, ZHANG Yourui. Changes of GH, INS, HC, TGF-β1 and IGF-1 levels in cows’ serum during milk withdrawal period[J]. , 2017, 29(4): 548-554.

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Changes of GH, INS, HC, TGF-β1 and IGF-1 levels in cows’ serum during milk withdrawal period

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