›› 2017, Vol. 29 ›› Issue (4): 555-559.DOI: 10.3969/j.issn.1004-1524.2017.04.06

• Animal Science • Previous Articles     Next Articles

Detection of the antibiotic genes of sulfonamides and quinolones of bacteria in refrigerated chicken

HE Xiangxiang1, 2, 3, YANG Hua2, 3, DAI Baoling2, 3, XIA Xiaodong1, XIAO Yingping2, 3, *   

  1. 1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China;
    2.Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    3. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:2016-11-07 Online:2017-04-20 Published:2017-04-27

Abstract: The long-term use of antibiotics in the animal farming industry has caused rapid expansion of drug resistant bacteria. These drug resistant bacteria are screened during the process of animal farming, and are easily transmitted in the animal production chain, which is a huge threat to human’s food safety. In this study, we aimed at testing the safety of refrigerated chicken collected from eight supermarkets and eleven agricultural markets in Shaoxing city, Zhejiang Province. We detected the antibiotic genes of sulfonamides and quinolones of surface microorganisms of the samples by PCR reaction and sequencing. We detected sul Ⅰ, sulⅡ, qnrD, qnrS, qepA and oqxB in the samples and the detection rate was 100%, 100%, 100%, 89.5%, 100% and 89.5%, respectively. We also sequenced the antibiotic genes with the sequences in NCBI and the coincidence rate was 100%, 99.44%, 100%, 92.74%, 99.82% and 100%, respectively. This study will provide effective support for risk assessment of antibiotic genes in the refrigerated chicken.

Key words: refrigerated chicken, bacteria, sulfonamides, quinolones, drug-resistance genes

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