Abstract: Analysis of codon usage bias of specific genes in strawberry transcriptome could provide reference for heterologous expression of genes. To understand the influence of red and blue light on codon usage bias, the original experimental data, which was transcriptome of strawberry treated with red and blue light, and the original control data, which was transcriptome of strawberry treated with white light, were screened by perl language and FPKM (fragments per kb per million fragments) value, then, Mobyle online program were used to analyze the codon usage bias of it. The results showed that there were 114 specific genes under the blue light, twice as that under the red light; From the point of view of GC content combined with GC3s (GC content of the third position), 91 percent of specific genes under red and blue light, respectively, were bias toward the synonymous codons with A and T at the third codon position; In addition, the fraction of specific genes under the red light between optimal codons with A and T at the third codon position and optimal codons was 2.84 percent more than that under the blue light. According to codon usage frequency, the Pichia pastoris expression system was more suitable for heterologous expression of specific genes of the two treatments, Arabidopsis thaliana might be the best receptor for transgenosis.

Key words: red and blue light, transcriptome, strawberry, codon usage bias