Cloning and expression analysis of GmDof1.5 in soybean under abiotic stress

doi:10.3969/j.issn.1004-1524.2021.01.01

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (1): 1-7.DOI: 10.3969/j.issn.1004-1524.2021.01.01

• Crop Science • Previous Articles Next Articles

Cloning and expression analysis of GmDof1.5 in soybean under abiotic stress

HE Jiaqi¹(), ZHAI Ying¹^,^*(), ZHANG Jun², QIU Shuang¹, LI Mingyang¹, ZHAO Yan¹, ZHANG Meijuan¹, MA Tianyi¹

1. Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, College of Life Science and Agro-Forestry, Qiqihar University, Qiqihar 161006, China
2. Institute of Animal Husbandry, Heilongjiang Institute of Veterinary Science, Qiqihar 161005, China

Received:2020-03-18 Online:2021-01-25 Published:2021-01-25
Contact: ZHAI Ying

Abstract

Abstract:

Dof transcription factors play an important regulatory role in plant growth and development and response to abiotic stress. A soybean Dof transcription factor GmDof1.5 with unknown function was cloned through RT-PCR, which was located on chromosome 15 of the soybean genome. The ORF of GmDof1.5 was 540 bp, which encoded 179 amino acid residues with molecular weight of 20.15 ku and isoelectric point of 9.82. Prediction of protein sequence showed that GmDof1.5 contained a typical Zf-Dof binding domain and a large number of phosphorylation sites. Phylogenetic analysis indicated that GmDof1.5 of soybean was highly homologous to SsDof4 of Spatholobus suberectus. Quantitative real-time PCR showed that the expressions of GmDof1.5 were induced by ABA, drought, salt, high temperature and cold stresses to different degrees. Especially, the response of GmDof1.5 to high temperature was the most obvious.

Key words: soybean, Dof transcription factor, abiotic stress, gene clone, expression analysis

CLC Number:

S565.1

HE Jiaqi, ZHAI Ying, ZHANG Jun, QIU Shuang, LI Mingyang, ZHAO Yan, ZHANG Meijuan, MA Tianyi. Cloning and expression analysis of GmDof1.5 in soybean under abiotic stress[J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 1-7.

Figures/Tables 4

Fig.1 PCR amplification of GmDof1.5 from soybean M, DL 2000 marker; 1-2, PCR product of GmDof1.5.

Fig.2 Nucleotide and deduced amino acid sequence of GmDof1.5 Zf-Dof domain was underlined; Phosphorylation sites were indicated by boldface type; Stop codon was shown in *.

Fig.3 Phylogenetic tree of Dof proteins

Fig.4 Expression of GmDof1.5 under drought(A), salt(B), high temperature(C), low temperature(D) and ABA(E) treatments in soybean seedlings ** and * indicate significant difference at P<0.01 and P<0.05, respectively.

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Cloning and expression analysis of GmDof1.5 in soybean under abiotic stress

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