Identification and bioinformatics analysis of alcohol dehydrogenase gene family of Setosphaeria turcica

doi:10.3969/j.issn.1004-1524.2023.05.14

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (5): 1108-1115.DOI: 10.3969/j.issn.1004-1524.2023.05.14

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Identification and bioinformatics analysis of alcohol dehydrogenase gene family of Setosphaeria turcica

ZHANG Shuhong(), ZHANG Yunfeng, WU Qiuying, GAO Fengju, LI Yazi, JI Jingxin, XU Ke, FAN Yongshan()

Department of Life Sciences, Tangshan Normal University, Tangshan Key Laboratory of Agricultural Pathogenic Fungi and Toxins, Tangshan 063000, Hebei, China

Received:2022-01-06 Online:2023-05-25 Published:2023-06-01

Abstract

Abstract:

Alcohol dehydrogenase (ADH) is a major enzyme of short chain alcohol metabolism in all organisms and plays an important role in the process of substance metabolism and energy conversion. Utilizing the KEGG database of Setosphaeria turcica genome, 22 members of the ADH gene family (StADH1-StADH22) of Setosphaeria turcica were obtained in this paper. The ADH genes were distributed over 12 scaffolds with 232-495 aa in length, the theoretical isoelectric point of 5.65-9.22, 81.8% acidic family members and 72.7% hydrophilic proteins. Four family members were located in mitochondria, 2 members in peroxisome, and the other 16 family members were located in cytoplasm. Phylogenetic analysis results show that there are 11 Setosphaeria turcica ADH family proteins closely related with yeast ADH gens (YADH1-7) which have the similar conservative motifs and structure domains, and the other 11 StADH family proteins are far from yeast ADH and contain different conservative motifs and structure domains. Through the analysis of Setosphaeria turcica-Zea mays gene expression transcriptome, there are four StADH family genes StADH1, StADH4, StADH6 and StADH12 participate in the pathogen infection process, and StADH1, StADH4 and StADH12 play very important roles in the later period of infection. Furthermore, StADH1 might be related to the pathogenicity specificity of the pathogen.

Key words: Setosphaeria turcica, alcohol dehydrogenase, gene family, transcriptome, pathogenicity

CLC Number:

S432.1

ZHANG Shuhong, ZHANG Yunfeng, WU Qiuying, GAO Fengju, LI Yazi, JI Jingxin, XU Ke, FAN Yongshan. Identification and bioinformatics analysis of alcohol dehydrogenase gene family of Setosphaeria turcica[J]. Acta Agriculturae Zhejiangensis, 2023, 35(5): 1108-1115.

Figures/Tables 5

Fig.1 Chromosome locations of ADH genes in Setosphaeria turcica

Fig.2 Phylogenetic tree of the ADH of Setosphaeria turcica

Fig.3 Analysis of the relative expression of ADH gene in Setosphaeria turcica at different time after inoculation

Fig.4 Expression of family members of ADH gene in Setosphaeria turcica after infection A,Inoculated disease-resistant inbred line (R); B,Inoculated susceptible inbred line (S); C,Difference of gene expression after 10 days of inoculation; * Indicating the significant difference(P<0.05).

Fig.5 Domain analysis of key genes of ADH gene family in Setosphaeria turcica

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Identification and bioinformatics analysis of alcohol dehydrogenase gene family of Setosphaeria turcica

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