Effects of exogenous spraying of amino acid fertilizer on growth and selenium uptake of watercress

doi:10.3969/j.issn.1004-1524.20221184

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (7): 1638-1647.DOI: 10.3969/j.issn.1004-1524.20221184

• Horticultural Science • Previous Articles Next Articles

Effects of exogenous spraying of amino acid fertilizer on growth and selenium uptake of watercress

XIAO Jiachang¹(), LEI Fengyun², GE Sang³, MA Junying¹, HE Maolin¹, LI Yanwen¹, ZHENG Yangxia¹^,^*()

1. College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China
2. Agricultural Equipment Research Institute, Chengdu Academy of Agriculture and Forestry Sciences, Chengdu 611130, China
3. Shannan Agricultural Technology Extension Center of Tibet Autonomous Region, Shannan 856000, Tibet, China

Received:2022-08-19 Online:2023-07-25 Published:2023-08-17
Contact: ZHENG Yangxia

Abstract

Abstract:

In order to improve the selenium absorption capacity of watercress (Nasturtium officinale R. Br.), pot experiments were conducted to investigate the effects of different concentrations of amino acid fertilizer (dilute 0, 600, 900, 1 200 and 1 500 times) on growth and selenium absorption of watercress under selenium treatment (5 mg·kg^-1). The results showed that exogenous application of amino acid fertilizer could increase the biomass of root and shoot of watercress, promote the content of chlorophyll and carotenoid in leaves, increase the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and reduce the content of soluble protein. The results of principal component analysis showed that 900 times dilution of amino acid fertilizer had the best growth promotion effect on watercress, and the root and shoot biomass increased significantly by 73.03% and 56.65% compared with the control, respectively. The application of 900-1 500 times dilution of amino acid fertilizer could also increase the total selenium accumulation in root, stem and leaf of watercress, but had no positive effect on the selenium content in root, stem, leaf and shoot of watercress. Correlation analysis showed that the biomass and total selenium accumulation of watercress were positively correlated with chlorophyll content, carotenoid content, POD activity, SOD activity, CAT activity and soil pH value. In conclusion, amino acid fertilizer can promote the growth of watercress and increase the total selenium accumulation in watercress.

Key words: amino acid fertilizer, watercress, biomass

CLC Number:

S645

XIAO Jiachang, LEI Fengyun, GE Sang, MA Junying, HE Maolin, LI Yanwen, ZHENG Yangxia. Effects of exogenous spraying of amino acid fertilizer on growth and selenium uptake of watercress[J]. Acta Agriculturae Zhejiangensis, 2023, 35(7): 1638-1647.

Figures/Tables 8

References 43

[1]	龚天芝, 张德健. 外源硒对核桃硒含量和果实品质的影响及生理作用机制[J]. 果树学报, 2022, 39(8): 1443-1449.
	GONG T Z, ZHANG D J. Effect of exogenous selenium application on selenium content and fruit quality as well as its physiological mechanism in walnut[J]. Journal of Fruit Science, 2022, 39(8): 1443-1449. (in Chinese with English abstract)
[2]	VINCETI M, FILIPPINI T, WISE L A. Environmental selenium and human health: an update[J]. Current Environmental Health Reports, 2018, 5(4): 464-485.
[3]	ALYEMENI M N, AHANGER M A, WIJAYA L, et al. Selenium mitigates cadmium-induced oxidative stress in tomato (Solanum lycopersicum L.) plants by modulating chlorophyll fluorescence, osmolyte accumulation, and antioxidant system[J]. Protoplasma, 2018, 255(2): 459-469.
[4]	JZWAIK W, MLECZEK M, POLITYCKA B. The effect of exogenous selenium on the growth and photosynthetic pigments content of cucumber seedlings[J]. Fresenius Environmental Bulletin, 2016, 25(1):142-152.
[5]	SAEEDI M, SOLTANI F, BABALAR M, et al. Selenium fortification alters the growth, antioxidant characteristics and secondary metabolite profiles of cauliflower (Brassica oleracea var. botrytis) cultivars in hydroponic culture[J]. Plants, 2021, 10(8): 1537.
[6]	梁乐, 刘娟, 李晓梅, 等. 三种基因型樱桃番茄混种对果实品质和硒含量的影响[J]. 浙江农业学报, 2021, 33(10): 1870-1878.
	LIANG L, LIU J, LI X M, et al. Effects of intercropping with different genotypes of cherry tomato on fruit quality and selenium content[J]. Acta Agriculturae Zhejiangensis, 2021, 33(10): 1870-1878. (in Chinese with English abstract)
[7]	ARAVIND P, PRASAD M N V. Cadmium-induced toxicity reversal by zinc in Ceratophyllum demersum L. (a free floating aquatic macrophyte) together with exogenous supplements of amino-and organic acids[J]. Chemosphere, 2005, 61(11): 1720-1733.
[8]	TALUKDER M R, ASADUZZAMAN M, TANAKA H, et al. Light-emitting diodes and exogenous amino acids application improve growth and yield of strawberry plants cultivated in recycled hydroponics[J]. Scientia Horticulturae, 2018, 239: 93-103.
[9]	ZHOU Z G, ZHOU J M, LI R Y, et al. Effect of exogenous amino acids on Cu uptake and translocation in maize seedlings[J]. Plant and Soil, 2007, 292(1): 105-117.
[10]	CERDÁN M, SÁNCHEZ-SÁNCHEZ A, OLIVER M, et al. Effect of foliar and root applications of amino acids on iron uptake by tomato plants[J]. Acta Horticulturae, 2009(830): 481-488.
[11]	ESKANDARI S, KHOSHGOFTARMANESH A H, SHARIFNABI B. The effect of foliar-applied manganese in mineral and complex forms with amino acids on certain defense mechanisms of cucumber (Cucumis sativus L.) against powdery mildew[J]. Journal of Plant Growth Regulation, 2018, 37(2): 481-490.
[12]	李杰, 卢宗云, 石元亮, 等. 新型聚氨酸增效肥料对小白菜根系活性与产量的影响[J]. 中国土壤与肥料, 2019(1): 134-139.
	LI J, LU Z Y, SHI Y L, et al. Effect of new type synergist of poly amino acid fertilizer on pakchoi root activity and yield[J]. Soil and Fertilizer Sciences in China, 2019(1): 134-139. (in Chinese with English abstract)
[13]	欧阳琳, 李春杰, 夏鲁卿, 等. 氨基酸肥料施用促进切花菊‘优香’养分吸收及品质优化[J]. 中国农业大学学报, 2015, 20(3): 90-99.
	OUYANG L, LI C J, XIA L Q, et al. Aminoacid fertilizer improved the nutrient uptake and the quality of cut Chrysanthemum Morifolum ‘Yuka’[J]. Journal of China Agricultural University, 2015, 20(3): 90-99. (in Chinese with English abstract)
[14]	KIM Y S, HAM S K, LEE J P, et al. Effects of two amino acid fertilizers on growth of creeping bentgrass and nitrogen uptake[J]. Weed & Turfgrass Science, 2014, 3(3): 246-252.
[15]	HUANG K W, LI K Q, SUI L Y, et al. Effects of different times of grafting on physiological characteristics and selenium absorption of watercress cuttings[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2020, 29(03):419-427.
[16]	高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006.
[17]	HUAN Y M, YANG L, LIU Q, et al. Effects of indole acetic acid on the growth and selenium absorption characteristics of Cyphomandra betacea seedlings[J]. Acta Physiologiae Plantarum, 2021, 43(5): 1-8.
[18]	李爱民, 范俊楠, 贺小敏, 等. 高效液相色谱-原子荧光光谱法测定土壤中4种有效硒形态[J]. 分析科学学报, 2021, 37(3): 403-407.
	LI A M, FAN J N, HE X M, et al. Determination of four available selenium species in soils by high performance liquid chromatography-atomic fluorescence spectrometry[J]. Journal of Analytical Science, 2021, 37(3): 403-407. (in Chinese with English abstract)
[19]	POORYOUSEF M, ALIZADEH K. Effect of foliar application of free amino acids on alfalfa performance under rainfed conditions[J]. Research on Crops, 2014, 15(1): 254.
[20]	操君喜, 彭智平, 黄继川, 等. 叶面施用氨基酸对菜心产量和品质的影响[J]. 中国农学通报, 2010, 26(4): 162-165.
	CAO J X, PENG Z P, HUANG J C, et al. Effect of foliar application of amino acid on yield and quality of flowering Chinese cabbage[J]. Chinese Agricultural Science Bulletin, 2010, 26(4): 162-165. (in Chinese with English abstract)
[21]	NASSAR A H, EL-TARABILY K A, SIVASITHAMPARAM K. Growth promotion of bean (Phaseolus vulgaris L.) by a polyamine-producing isolate of Streptomyces griseoluteus[J]. Plant Growth Regulation, 2003, 40(2): 97-106.
[22]	AMIN A A, GHARIB F A E, EL-AWADI M, et al. Physiological response of onion plants to foliar application of putrescine and glutamine[J]. Scientia Horticulturae, 2011, 129(3): 353-360.
[23]	YARONSKAYA E, VERSHILOVSKAYA I, POERS Y, et al. Cytokinin effects on tetrapyrrole biosynthesis and photosynthetic activity in barley seedlings[J]. Planta, 2006, 224(3): 700-709.
[24]	JO G W, KIM Y S, HAM S K, et al. Growth and quality changes of creeping bentgrass by application of keratin amino acid fertilizer[J]. Weed & Turfgrass Science, 2016, 5(4): 260-267.
[25]	CERDÁN M, SÁNCHEZ-SÁNCHEZ A, JORDÁ J D, et al. Effect of commercial amino acids on iron nutrition of tomato plants grown under lime-induced iron deficiency[J]. Journal of Plant Nutrition and Soil Science, 2013, 176(6): 859-866.
[26]	王孝娣, 刘凤之, 王帅, 等. 氨基酸硒和6-BA对葡萄叶片衰老和叶绿体超微结构的影响[J]. 中国果树, 2019(6): 49-53, 64, 124.
	WANG X D, LIU F Z, WANG S, et al. Effects of amino acid selenium and 6-BA on leaf senescence and chloroplast ultrastructure of grape[J]. China Fruits, 2019(6): 49-53, 64, 124. (in Chinese with English abstract)
[27]	申明, 成学慧, 谢荔, 等. 氨基酸叶面肥对砂梨叶片光合作用的促进效应[J]. 南京农业大学学报, 2012, 35(2): 81-86.
	SHEN M, CHENG X H, XIE L, et al. Effects of amino-acid fertilizers on photosynthesis in leaves of pear cultivars[J]. Journal of Nanjing Agricultural University, 2012, 35(2): 81-86. (in Chinese with English abstract)
[28]	ANWAR A, YAN Y, LIU Y M, et al. 5-Aminolevulinic acid improves nutrient uptake and endogenous hormone accumulation, enhancing low-temperature stress tolerance in cucumbers[J]. International Journal of Molecular Sciences, 2018, 19(11): 3379.
[29]	杨雅兰, 王诗赞, 田旖, 等. ALA和氨基酸肥料对桃光合特性及品质的影响[J]. 分子植物育种, 2022, 20(6): 1930-1936.
	YANG Y L, WANG S Z, TIAN Y, et al. Effects of ALA and amino acid fertilizers on leaf photosynthesis and quality of peach[J]. Molecular Plant Breeding, 2022, 20(6): 1930-1936. (in Chinese with English abstract)
[30]	ANWAR A, WANG J, YU X C, et al. Substrate application of 5-aminolevulinic acid enhanced low-temperature and weak-light stress tolerance in cucumber (Cucumis sativus L.)[J]. Agronomy, 2020, 10(4): 472.
[31]	WANG J W, ZHANG J, LI J, et al. Exogenous application of 5-aminolevulinic acid promotes coloration and improves the quality of tomato fruit by regulating carotenoid metabolism[J]. Frontiers in Plant Science, 2021, 12: 683868.
[32]	WU Y E, JIN X, LIAO W B, et al. 5-aminolevulinic acid (ALA) alleviated salinity stress in cucumber seedlings by enhancing chlorophyll synthesis pathway[J]. Frontiers in Plant Science, 2018, 9: 635.
[33]	MENG L N, CHEN J P, GAN W C, et al. Effects of amino acid selenium foliar fertilizer on selenium content and quality of mango[J]. Agricultural Biotechnology, 2020, 9(6):92-94.
[34]	SONG H M, XU X B, WANG H A, et al. Exogenous γ-aminobutyric acid alleviates oxidative damage caused by aluminium and proton stresses on barley seedlings[J]. Journal of the Science of Food and Agriculture, 2010, 90(9): 1410-1416.
[35]	WANG W, CANG L, ZHOU D M, et al. Exogenous amino acids increase antioxidant enzyme activities and tolerance of rice seedlings to cadmium stress[J]. Environmental Progress & Sustainable Energy, 2017, 36(1): 155-161.
[36]	许猛, 袁亮, 李伟, 等. 复合氨基酸肥料增效剂对NaCl胁迫下小白菜种子萌发和苗期生长的影响[J]. 植物营养与肥料学报, 2018, 24(4): 992-1000.
	XU M, YUAN L, LI W, et al. Effects of a fertilizer synergist containing compound amino acids on seed germination and seedling growth of pakchoi under NaCl stress[J]. Journal of Plant Nutrition and Fertilizers, 2018, 24(4): 992-1000. (in Chinese with English abstract)
[37]	RAHMANTO A S, DAVIES M J. Selenium-containing amino acids as direct and indirect antioxidants[J]. IUBMB Life, 2012, 64(11): 863-871.
[38]	KOSKAN L P, MEAH A R Y, SANDERS L J, et al. Method and composition for enhanced plant productivity comprising fertilizer and cross-linked polyamino acid: US5861356[P/OL]. (1999-01-19)[2022-08-17].https://www.freepatentsonline.com/5861356.html.
[39]	宋奇超, 曹凤秋, 巩元勇, 等. 高等植物氨基酸吸收与转运及生物学功能的研究进展[J]. 植物营养与肥料学报, 2012, 18(6): 1507-1517.
	SONG Q C, CAO F Q, GONG Y Y, et al. Current research progresses of amino acids uptake, transport and their biological roles in higher plants[J]. Plant Nutrition and Fertilizer Science, 2012, 18(6): 1507-1517. (in Chinese with English abstract)
[40]	胡开博, 杨清夏, 李扬, 等. 化肥减氮配施氨基酸肥料对春玉米生产的影响[J]. 浙江农业学报, 2022, 34(4): 661-670.
	HU K B, YANG Q X, LI Y, et al. Effect of application of amino acid fertilizer on spring maize cultivation under nitrogen reduction[J]. Acta Agriculturae Zhejiangensis, 2022, 34(4): 661-670. (in Chinese with English abstract)
[41]	HU M M, DOU Q H, CUI X M, et al. Polyaspartic acid mediates the absorption and translocation of mineral elements in tomato seedlings under combined copper and cadmium stress[J]. Journal of Integrative Agriculture, 2019, 18(5): 1130-1137.
[42]	SAMANE E. Translocation, leaf distribution, and nutritional status of manganese in cucumber plants as affected by foliar application of exogenous amino acids[J]. Journal of Plant Growth Regulation, 2020, 39(3): 1191-1204.
[43]	JI X J, XIONG S P, LI C M, et al. Effects of different fertilizer types on temporal and spatial changes of soil enzyme activities and microbial population[J]. Journal of Soil and Water Conservation, 2008(1):123-127.

氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	每株生物量Biomass per plant/g
氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	根Root	茎Stem	叶Leaf	地上部Shoot	根冠比Root/shoot ratio
0	0.152±0.004 d	0.260±0.011 d	0.545±0.010 c	0.805±0.021 d	0.189±0.008 b
600	0.158±0.003 cd	0.388±0.007 c	0.577±0.017 c	0.965±0.024 c	0.164±0.001 c
900	0.263±0.005 a	0.453±0.013 a	0.808±0.018 a	1.261±0.031 a	0.209±0.007 a
1 200	0.225±0.003 b	0.433±0.008 ab	0.750±0.011 b	1.183±0.020 b	0.190±0.005 b
1 500	0.165±0.004 c	0.420±0.006 b	0.740±0.016 b	1.160±0.021 b	0.142±0.005 d

氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	每株生物量Biomass per plant/g
氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	根Root	茎Stem	叶Leaf	地上部Shoot	根冠比Root/shoot ratio
0	0.152±0.004 d	0.260±0.011 d	0.545±0.010 c	0.805±0.021 d	0.189±0.008 b
600	0.158±0.003 cd	0.388±0.007 c	0.577±0.017 c	0.965±0.024 c	0.164±0.001 c
900	0.263±0.005 a	0.453±0.013 a	0.808±0.018 a	1.261±0.031 a	0.209±0.007 a
1 200	0.225±0.003 b	0.433±0.008 ab	0.750±0.011 b	1.183±0.020 b	0.190±0.005 b
1 500	0.165±0.004 c	0.420±0.006 b	0.740±0.016 b	1.160±0.021 b	0.142±0.005 d

氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	叶绿素a含量 Chlorophyll a content	叶绿素b含量 Chlorophyll b content	总叶绿素含量 Total chlorophyll content	类胡萝卜素含量 Carotenoid content
0	0.291±0.005 c	0.130±0.002 b	0.421±0.007 c	0.044±0.005 c
600	0.312±0.006 b	0.135±0.006 ab	0.447±0.011 bc	0.047±0.003 c
900	0.355±0.007 a	0.148±0.002 a	0.503±0.009 a	0.061±0.003 a
1 200	0.350±0.006 a	0.147±0.008 a	0.497±0.014 a	0.057±0.004 ab
1 500	0.329±0.009 b	0.146±0.006 a	0.475±0.015 ab	0.050±0.001 bc

氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	叶绿素a含量 Chlorophyll a content	叶绿素b含量 Chlorophyll b content	总叶绿素含量 Total chlorophyll content	类胡萝卜素含量 Carotenoid content
0	0.291±0.005 c	0.130±0.002 b	0.421±0.007 c	0.044±0.005 c
600	0.312±0.006 b	0.135±0.006 ab	0.447±0.011 bc	0.047±0.003 c
900	0.355±0.007 a	0.148±0.002 a	0.503±0.009 a	0.061±0.003 a
1 200	0.350±0.006 a	0.147±0.008 a	0.497±0.014 a	0.057±0.004 ab
1 500	0.329±0.009 b	0.146±0.006 a	0.475±0.015 ab	0.050±0.001 bc

氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	POD活性 POD activity/ (U·g^-1min^-1)	SOD活性 SOD activity/ (U·g^-1min^-1)	CAT活性 CAT activity/ (U·g^-1min^-1)	可溶性蛋白含量 Soluble protein content/ (mg·g^-1)
0	1 255±7.78 d	190.5±7.02 c	2.003±0.070 e	80.89±4.72 a
600	1 300±9.90 c	205.7±4.79 b	2.270±0.062 d	74.41±2.42 a
900	1 474±2.83 a	234.3±4.79 a	5.348±0.072 a	52.92±3.71 c
1 200	1 459±12.73 a	213.0±4.04 b	5.047±0.088 b	58.47±2.94 bc
1 500	1 378±16.97 b	207.6±2.34 b	2.843±0.087 c	62.30±2.38 b

Effects of exogenous spraying of amino acid fertilizer on growth and selenium uptake of watercress

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 43

Related Articles 15

Recommended Articles

Metrics

Comments

项目 Items	成分Component
项目 Items	F₁	F₂
根系生物量Root biomass	0.913	0.361
地上部分生物量Shoot biomass	0.968	-0.231
叶绿素a含量Chlorophyll a content	0.996	-0.057
叶绿素b含量Chlorophyll b content	0.940	-0.332
总叶绿素含量Total chlorophyll content	0.991	-0.123
类胡萝卜素含量Carotenoid content	0.980	0.165
POD活性POD activity	0.988	-0.050
SOD活性SOD activity	0.932	0.216
CAT活性CAT activity	0.943	0.203
可溶性蛋白含量Soluble protein content	-0.984	0.137
根系硒含量Se content in root	-0.635	0.770
地上部分硒含量Se content in shoot	-0.977	0.119
根系硒总积累量Se accumulation in root	0.651	0.733
地上部分硒总积累量	0.881	-0.474
Se accumulation in shoot
土壤有效硒含量Soil available selenium	-0.870	-0.415
土壤pH值Soil pH value	0.757	0.263
特征值Eigenvalue	13.18	2.06
贡献率Eontribution rate/%	82.35	12.87
累计贡献率Eumulative contribution rate/%	82.35	95.23

[1]	WANG Weiwei, MEI Yi, WU Yongcheng, WAN Hongjian, CHEN Changjun, ZHENG Qingsong, ZHENG Jiaqiu. Effects of corncob biochar application on soil characteristics and pepper growth under continuous cropping [J]. Acta Agriculturae Zhejiangensis, 2023, 35(1): 156-163.
[2]	WU Hao, ZHANG Xuesong, WANG Dan. Effects of different CO₂ concentration and nitrogen rates on photosynthesis and growth of winter wheat [J]. Acta Agriculturae Zhejiangensis, 2022, 34(12): 2594-2602.
[3]	HOU Lijuan, LI Zhengpeng, LIN Jinsheng, MA Lin, LI Huiping, QU Shaoxuan, JIANG Jianxin, ZOU Xiulong, YANG Huaping, LI Changtian, JIANG Ning. Effects of different light quality of LED light source on growth rate, mycelium branch and biomass of straw mushroom mycelium [J]. Acta Agriculturae Zhejiangensis, 2021, 33(6): 1110-1116.
[4]	LIAN Huashan, LI Xinxin, LIN Lijin, LIAO Ming’an. Study on epibrassinolide (EBR) on growth and physiological response of Summer Black grape seedlings [J]. Acta Agriculturae Zhejiangensis, 2021, 33(10): 1889-1896.
[5]	LI Le, TIAN Minjiao, GAO Yanming, LI Jianshe. Effect of selenium fertilizer on growth and mineral element accumulation of tomato in substrate culture [J]. , 2020, 32(2): 253-261.
[6]	HOU Lijuan, LIN Jinsheng, LIU Shaohua, LI Ruixiang, MA Lin, JIANG Ning, QU Shaoxuan, LI Huiping. Sodium acetate and compound with four kinds of minerals on the mycelium biomass of Volvariella volvacea [J]. , 2018, 30(2): 228-235.
[7]	XU Chenyang, MAO Xiaoyu. Effects of different concentrations of acid rain on biomass allocation and physiological characteristics in Morus alba seedlings under cadmium pollution [J]. , 2018, 30(12): 2112-2120.
[8]	WANG Yangyang, SUN Wei, LI Gan, KANG Zhenghua, MIAO Jian. Rapid and non-destructive estimation method for aboveground biomass of Tamarix ramosissima based on Android platform [J]. , 2017, 29(11): 1920-1929.
[9]	XU Wei-hui, WU Feng-zhi. Response of soil enzymes activities and microorganism in rhizosphere of watermelon to wheat as companion crop [J]. , 2016, 28(9): 1588-1594.
[10]	LIN Xiaoqing1，2， LYU Haohao2，3， LIU Yuxue2，3， WANG Yuying2，3， YANG Shengmao1，2，3，*. Effects of biomass and carbonization temperature on biochar yield and characteristics [J]. , 2016, 28(7): 1216-.
[11]	SHAO Yangfeng1， MEI Hongfei1， PAN zhongchao1， LIU Huan2， WANG Chaoqi2. Effects of corn straw returning on soil organic carbon content， microbial functional diversity and cabbage yield [J]. , 2016, 28(5): 838-.
[12]	GAO Linan. Influence of water quality on growth and chlorophyll fluorescence parameters of Hippuris vulgaris in Jiuzhaigou National Nature Reserve [J]. , 2016, 28(5): 857-.
[13]	MA Shuai1， FENG Jin\\|chao1，*， GONG Ting\\|ting1， WU Li\\|ji2， Li Yu\\|xian1， FENG Ya\\|lei1， ZHAO Hui\\|qing3. Soil respiration feature of 4 types of grassland in growing season in Hulun Lake， Inner Mongolia#br# [J]. , 2015, 27(7): 1221-.
[14]	ZENG Yan-ping;MA Jia-hai*;CHEN Bin-bin;CAI Yong-chao;GAO Song. Survey on the community of benthic macro-algae in Gouqi island of Zhejiang Province [J]. , 2013, 25(5): 0-1102.
[15]	YUAN Hui-fang;WEI Li-ping;TIAN Yao-hua;YUE Hai. Effects of shading on seedling growth and leaf anatomical characteristics of Aquilaria sinensis [J]. , 2013, 25(4): 0-752.

氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	硒含量Selenium content
氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	根Root	茎Stem	叶Leaf	地上部Shoot
0	4.090±0.047 a	0.895±0.025 a	1.376±0.036 a	2.271±0.044 a
600	3.778±0.105 b	0.800±0.008 b	1.286±0.029 a	2.087±0.015 b
900	3.505±0.107 c	0.722±0.022 c	1.032±0.040 c	1.754±0.044 d
1 200	3.173±0.068 d	0.760±0.027 bc	1.124±0.034 bc	1.883±0.005 c
1 500	2.913±0.060 e	0.759±0.035 bc	1.163±0.055 b	1.922±0.014 c

氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	硒含量Selenium content
氨基酸肥稀释倍数 Dilution ratio of amino acid fertilizer	根Root	茎Stem	叶Leaf	地上部Shoot
0	4.090±0.047 a	0.895±0.025 a	1.376±0.036 a	2.271±0.044 a
600	3.778±0.105 b	0.800±0.008 b	1.286±0.029 a	2.087±0.015 b
900	3.505±0.107 c	0.722±0.022 c	1.032±0.040 c	1.754±0.044 d
1 200	3.173±0.068 d	0.760±0.027 bc	1.124±0.034 bc	1.883±0.005 c
1 500	2.913±0.060 e	0.759±0.035 bc	1.163±0.055 b	1.922±0.014 c