Effects of melatonin seed priming on growth and physiological characteristics of Capsicum annuum under drought stress

doi:10.3969/j.issn.1004-1524.20221227

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (10): 2378-2388.DOI: 10.3969/j.issn.1004-1524.20221227

• Horticultural Science • Previous Articles Next Articles

Effects of melatonin seed priming on growth and physiological characteristics of Capsicum annuum under drought stress

WEI Xiya(), LIANG Lamei, LIN Xinqi, QIN Zhongwei, LI Yingzhi()

Binhai Agricultural College of Guangdong Ocean University, Zhanjiang 524000, Guangdong, China

Received:2022-08-21 Online:2023-10-25 Published:2023-10-31

Abstract

Abstract:

In order to explore the regulatory effect of melatonin seed priming treatment on drought tolerance of pepper, the effects of 10 different concentrations of melatonin seed priming treatment on plant growth and physiological characteristics of pepper were analyzed by pot PEG simulating drought stress with Maoshu 360 as the material. The results showed that the root length, plant height, root-shoot ratio, root fresh weight, shoot fresh weight, shoot dry weight and root dry weight increased firstly and then decreased with the increase of starter solution concentration, and the plant height, shoot fresh weight and shoot dry weight reached the maximum when the concentration was 100 μmol·L^-1. The root length, root shoot ratio, root fresh weight and root dry weight reached the maximum when the concentration was 75 μmol·L^-1. Physiological analysis showed that the contents of malondialdehyde, hydrogen peroxide and superoxide anion were the lowest, while the contents of soluble sugar, soluble protein, proline, AsA, DHA and antioxidant enzyme activities (POD, SOD, APX and GR) were the highest in leaves under drought stress induced by 100 μmol·L^-1 melatonin. After 75 μmol·L^-1 melatonin, the contents of malondialdehyde, hydrogen peroxide, and superoxide anion were the lowest, and the soluble sugar, soluble protein, proline contents, antioxidant enzymes (POD, SOD, CAT, APX and GR) activities, AsA, DHA contents and AsA/DHA in roots were the highest. The activity of CAT and contents of soluble protein and AsA/DHA in leaves were the highest. These results indicated that 100 μmol·L^-1 and 75 μmol·L^-1 melatonin seed priming treatment was beneficial to improve drought resistance of pepper.

Key words: drought stress, pepper, melatonin, leaf, root

CLC Number:

S641.3

WEI Xiya, LIANG Lamei, LIN Xinqi, QIN Zhongwei, LI Yingzhi. Effects of melatonin seed priming on growth and physiological characteristics of Capsicum annuum under drought stress[J]. Acta Agriculturae Zhejiangensis, 2023, 35(10): 2378-2388.

Figures/Tables 7

Table 1 Effects of seed priming treatments with different concentrations of melatonin on plant height, root length and root-shoot ratio of pepper under drought stress

处理Treatment	株高Plant height/cm	根长Root length/cm	根冠比Root-shoot ratio
CK	11.034±0.061 e	12.687±0.483 g	1.150±0.043 f
T0	11.829±0.158 d	15.679±0.774 f	1.328±0.083 e
T1	12.260±0.192 cd	20.232±0.430 e	1.652±0.058 bcd
T5	12.629±0.302 bc	19.983±0.218 e	1.583±0.024 cd
T25	12.954±0.325 bc	22.902±0.700 cd	1.770±0.072 ab
T50	13.349±0.290 b	23.180±0.875 bc	1.736±0.048 abc
T75	14.486±0.362 a	26.794±0.433 a	1.852±0.055 a
T100	15.042±0.164 a	24.867±0.148 b	1.653±0.009 bcd
T125	12.718±0.346 bc	21.211±0.669 de	1.668±0.012 bcd
T150	10.838±0.101 e	16.381±0.772 f	1.512±0.077 d

Table 2 Effects of melatonin seed priming treatments with different concentrations on fresh weight and dry weight of root and shoot of pepper under drought stress g

处理 Treatment	根鲜重 Root fresh weight	地上部鲜重 Shoot fresh weight	根干重 Root dry weight	地上部干重 Shoot dry weight
CK	1.359±0.053 e	1.583±0.055 fg	0.072±0.005 f	0.115±0.006 f
T0	1.552±0.04 d	1.766±0.081 e	0.102±0.005 de	0.216±0.007 e
T1	1.596±0.035 d	1.731±0.005 ef	0.114±0.007 d	0.206±0.008 e
T5	1.574±0.048 d	1.720±0.050 ef	0.134±0.005 c	0.241±0.015 e
T25	1.932±0.041 c	2.286±0.056 d	0.145±0.004 c	0.290±0.015 d
T50	2.101±0.052 b	2.31±0.048 cd	0.167±0.003 b	0.323±0.007 cd
T75	2.401±0.035 a	2.455±0.079 bc	0.193±0.007 a	0.471±0.014 b
T100	2.313±0.029 a	2.969±0.015 a	0.185±0.007 a	0.509±0.020 a
T125	1.978±0.060 bc	2.549±0.014 b	0.166±0.003 b	0.462±0.011 b
T150	1.065±0.037 f	1.484±0.080 g	0.097±0.006 e	0.355±0.013 c

Fig.1 Effects of seed priming treatments with different concentrations of melatonin on MDA (A), hydrogen peroxide (B) and superoxide anion (C) contents in leaves and roots of pepper under drought stress The treatments of the same tissue with different lowercase letters show the significant difference(P<0.05). The same as below.

Fig.2 Effects of seed priming with different concentrations of melatonin on the contents of soluble protein (A), soluble sugar (B) and proline (C) in leaves and roots of pepper under drought stress

Fig.3 Effects of seed priming with different concentrations of melatonin on POD (A), SOD (B) and CAT (C) activities in leaves and roots of pepper under drought stress

Fig.4 Effects of seed priming treatments with different concentrations of melatonin on APX (A) and GR (B) activities in roots and leaves of pepper under drought stress

Fig.5 Effects of seed priming treatments with different concentrations of melatonin on contents of AsA (A), DHA (B) and AsA/DHA(C) in leaves and roots of pepper under drought stress

References 37

[1]	龚记熠, 彭毅秋, 张冬林, 等. 干旱胁迫对引种辣椒生长特性的影响[J]. 广东农业科学, 2014, 41(2): 54-56, 69.
	GONG J Y, PENG Y Q, ZHANG D L, et al. Effects of drought stress on growth characteristics of introduced pepper[J]. Guangdong Agricultural Sciences, 2014, 41(2): 54-56, 69. (in Chinese with English abstract)
[2]	欧立军, 陈波, 邹学校. 干旱对辣椒光合作用及相关生理特性的影响[J]. 生态学报, 2012, 32(8): 2612-2619.
	OU L J, CHEN B, ZOU X X. Effects of drought stress on photosynthesis and associated physiological characters of pepper[J]. Acta Ecologica Sinica, 2012, 32(8): 2612-2619. (in Chinese with English abstract)
[3]	刘晓建, 谢小玉, 薛兰兰. 辣椒开花结果期对干旱胁迫响应机制的研究[J]. 西北农业学报, 2009, 18(5): 246-249.
	LIU X J, XIE X Y, XUE L L. Responses of pepper during blossom and bear fruit under drought stress[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2009, 18(5): 246-249. (in Chinese with English abstract)
[4]	胡能兵, 隋益虎, 舒英杰, 等. 高温干旱胁迫对辣椒热害指标及叶绿素荧光参数F_v/F_m的影响[J]. 基因组学与应用生物学, 2018, 37(12): 5421-5428.
	HU N B, SUI Y H, SHU Y J, et al. Effects of heat an d drought stresses on heat damage indexes and chlorophyll fluorescence parameter F_v/F_mof pepper[J]. Genomics and Applied Biology, 2018, 37(12): 5421-5428. (in Chinese with English abstract)
[5]	刘颖, 白春雷, 魏春光, 等. PEG干旱胁迫对内蒙古自治区辣椒苗期生理指标影响[J]. 分子植物育种, 2023, 21(9): 3036-3041.
	LIU Y, BAI C L, WEI C G, et al. Effects of PEG drought stress on physiological indexes of pepper seedlings in inner Mongolia[J]. Molecular Plant Breeding, 2023, 21(9): 3036-3041. (in Chinese with English abstract)
[6]	刘佳, 郁继华, 徐秉良, 等. 干旱气候条件下水分胁迫对辣椒叶片生理特性的影响[J]. 核农学报, 2012, 26(8): 1197-1203.
	LIU J, YU J H, XU B L, et al. Effects of water stress on the physiological characteristics of pepper leaves in arid climates[J]. Journal of Nuclear Agricultural Sciences, 2012, 26(8): 1197-1203. (in Chinese with English abstract)
[7]	吴天珍, 祁光斌, 颉建明, 等. 干旱胁迫下CoCl₂对辣椒幼苗生长及部分生理特性的影响[J]. 甘肃农业大学学报, 2016, 51(3): 55-59.
	WU T Z, QI G B, XIE J M, et al. Effect of CoCl₂on growth and physiological characteristics of pepper seedlings under drought stress[J]. Journal of Gansu Agricultural University, 2016, 51(3): 55-59. (in Chinese with English abstract)
[8]	潘红艳, 张晓庆, 李婕, 等. 褪黑素对低温胁迫后菘蓝种子苗抗氧化性影响[J]. 西北大学学报(自然科学版), 2013, 43(2): 238-242.
	PAN H Y, ZHANG X Q, LI J, et al. Effects of exogenous melatonin on antioxidant activities in Isatis indigotica Fort. seedlings after low temperature stress[J]. Journal of Northwest University (Natural Science Edition), 2013, 43(2): 238-242. (in Chinese with English abstract)
[9]	GALANO A, TAN D X, REITER R J. Melatonin as a natural ally against oxidative stress: a physicochemical examination[J]. Journal of Pineal Research, 2011, 51(1): 1-16.
[10]	唐懿, 任纬, 刘副刚, 等. 褪黑素浸种对豌豆幼苗生长及镉积累的影响[J]. 土壤, 2018, 50(1): 109-114.
	TANG Y, REN W, LIU F G, et al. Effects of melatonin soaking on growth and cadmium accumulation of pea seedlings[J]. Soils, 2018, 50(1): 109-114. (in Chinese with English abstract)
[11]	孙浩月, 吴洪斌, 李明, 等. 褪黑素浸种对盐胁迫下芸豆幼苗生长及生理特性的影响[J]. 河南农业科学, 2021, 50(12): 111-120.
	SUN H Y, WU H B, LI M, et al. Effects of melatonin seed soaking on growth and physiological characteristics of kidney bean seedlings under salt stress[J]. Journal of Henan Agricultural Sciences, 2021, 50(12): 111-120. (in Chinese with English abstract)
[12]	林晓金, 付尧, 李林, 等. 褪黑素浸种对宁粳8号水稻机插植伤修复效应研究[J]. 现代农业科技, 2018(19): 9-11, 13.
	LIN X J, FU Y, LI L, et al. Study on the effect of melatonin soaking seeds on the repair of machine transplantation injury in rice variety Ningjing 8[J]. Modern Agricultural Science and Technology, 2018(19): 9-11, 13. (in Chinese)
[13]	刘金海, 蒋金娟, 罗富成, 等. 外源二聚丙三醇对非洲狗尾草种子萌发及幼苗生长的影响[J]. 草地学报, 2022, 30(4): 950-956.
	LIU J H, JIANG J J, LUO F C, et al. Effects of exogenous diglycerol treatment on seed germination and seedling growth of Setaria sphacelata ‘Narok’[J]. Acta Agrestia Sinica, 2022, 30(4): 950-956. (in Chinese with English abstract)
[14]	刘家尧, 刘新. 植物生理学实验教程[M]. 北京: 高等教育出版社, 2010.
[15]	NAKANO Y, ASADA K. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts[J]. Plant and Cell Physiology, 1981, 22(5): 867-880.
[16]	王俊力, 王岩, 赵天宏, 等. 臭氧胁迫对大豆叶片抗坏血酸-谷胱甘肽循环的影响[J]. 生态学报, 2011, 31(8): 2068-2075.
	WANG J L, WANG Y, ZHAO T H, et al. Effects of ozone on AsA-GSH cycle in soybean leaves[J]. Acta Ecologica Sinica, 2011, 31(8): 2068-2075. (in Chinese with English abstract)
[17]	杜欢, 马彤彤, 郭帅, 等. 大麦近等基因系苗期根系形态及叶片渗透调节物质对PEG胁迫的响应[J]. 中国农业科学, 2017, 50(13): 2423-2432.
	DU H, MA T T, GUO S, et al. Response of root morphology and leaf osmoregulation substances of seedling in barley genotypes with different heights to PEG stress[J]. Scientia Agricultura Sinica, 2017, 50(13): 2423-2432. (in Chinese with English abstract)
[18]	马旭辉, 陈茹梅, 柳小庆, 等. 褪黑素对玉米幼苗根系发育和抗旱性的影响[J]. 生物技术通报, 2021, 37(2): 1-14.
	MA X H, CHEN R M, LIU X Q, et al. Effects of melatonin on root growth and drought tolerance of maize seedlings[J]. Biotechnology Bulletin, 2021, 37(2): 1-14. (in Chinese with English abstract)
[19]	秦彬, 张明聪, 何松榆, 等. 褪黑素浸种对大豆种子萌发过程中干旱胁迫的缓解效应[J]. 干旱地区农业研究, 2020, 38(2): 192-198.
	QIN B, ZHANG M C, HE S Y, et al. Alleviating effect of melatonin soaking on drought stress during soybean seed germination[J]. Agricultural Research in the Arid Areas, 2020, 38(2): 192-198. (in Chinese with English abstract)
[20]	刘思奇. 美丽箬竹光合特性和抗氧化系统对干旱高温胁迫的响应[D]. 杭州: 浙江农林大学, 2021.
	LIU S Q. Responses of photosynthetic characteristics and antioxidant system of Indocalamus decorus to drought and high temperature stress[D]. Hangzhou: Zhejiang A & F University, 2021. (in Chinese with English abstract)
[21]	张明生, 谢波, 谈锋, 等. 甘薯可溶性蛋白、叶绿素及ATP含量变化与品种抗旱性关系的研究[J]. 中国农业科学, 2003, 36(1): 13-16.
	ZHANG M S, XIE B, TAN F, et al. Relationship among soluble protein, chlorophyll and ATP in sweet potato under water stress with drought resistance[J]. Scientia Agricultura Sinica, 2003, 36(1): 13-16. (in Chinese with English abstract)
[22]	史玉炜, 王燕凌, 李文兵, 等. 水分胁迫对刚毛柽柳可溶性蛋白、可溶性糖和脯氨酸含量变化的影响[J]. 新疆农业大学学报, 2007, 30(2): 5-8.
	SHI Y W, WANG Y L, LI W B, et al. Effects of water stress on soluble protein, soluble sugar and proline content in Tamarix hispida[J]. Journal of Xinjiang Agricultural University, 2007, 30(2): 5-8. (in Chinese with English abstract)
[23]	马艳丽. 水分胁迫对3个枣品种可溶性糖含量的影响[J]. 防护林科技, 2020(3): 23-24.
	MA Y L. Effect of water stress on soluble sugar content of three Ziziphus jujuba varieties[J]. Protection Forest Science and Technology, 2020(3): 23-24. (in Chinese with English abstract)
[24]	朱虹, 祖元刚, 王文杰, 等. 逆境胁迫条件下脯氨酸对植物生长的影响[J]. 东北林业大学学报, 2009, 37(4): 86-89.
	ZHU H, ZU Y G, WANG W J, et al. Effect of proline on plant growth under different stress conditions[J]. Journal of Northeast Forestry University, 2009, 37(4): 86-89. (in Chinese with English abstract)
[25]	苏文欣, 许凌欣, 姜宛彤, 等. 不同外源物质对盐碱胁迫下紫苏种子萌发、幼苗生长及生理的影响[J]. 草地学报, 2022, 30(9): 2415-2422.
	SU W X, XU L X, JIANG W T, et al. Effects of different exogenous substances on seed germination and seedling growth and physiology of Perilla frutescens under saline-alkali stress[J]. Acta Agrestia Sinica, 2022, 30(9): 2415-2422. (in Chinese with English abstract)
[26]	习林杰. 盐胁迫下外源褪黑素对生菜幼苗根系的影响[D]. 杨凌: 西北农林科技大学, 2019.
	Ⅺ L J. Effects of exogenous melatonin on root system of lettuce seedlings under salt stress[D]. Yangling: Northwest A & F University, 2019. (in Chinese with English abstract)
[27]	赵梅, 周瑞莲, 刘建芳, 等. 冬季融冻过程中白三叶叶片抗氧化酶活力和渗透调节物含量变化与抗冻性的关系[J]. 生态学报, 2011, 31(2): 306-315.
	ZHAO M, ZHOU R L, LIU J F, et al. The relationship between freeze-tolerance and changes in activities of antioxidant enzymes and osmolyte content in the leaves of white clover during early winter freeze-thaw cycles[J]. Acta Ecologica Sinica, 2011, 31(2): 306-315. (in Chinese with English abstract)
[28]	杨叶萍, 简敏菲, 余厚平, 等. 镉胁迫对苎麻(Boehmeria nivea)根系及叶片抗氧化系统的影响[J]. 生态毒理学报, 2016, 11(4): 184-193.
	YANG Y P, JIAN M F, YU H P, et al. Influence on the antioxidant system in roots and leaves of Boehmeria nivea under different cadmium stress[J]. Asian Journal of Ecotoxicology, 2016, 11(4): 184-193. (in Chinese with English abstract)
[29]	王译, 韩莹琰, 郝敬虹, 等. 褪黑素对高温胁迫下生菜抗氧化酶系统的影响[J]. 北京农学院学报, 2022, 37(2): 45-49.
	WANG Y, HAN Y Y, HAO J H, et al. Effects of melatonin on antioxidant enzyme of lettuce under high temperature stress[J]. Journal of Beijing University of Agriculture, 2022, 37(2): 45-49. (in Chinese with English abstract)
[30]	李泽琴, 李静晓, 张根发. 植物抗坏血酸过氧化物酶的表达调控以及对非生物胁迫的耐受作用[J]. 遗传, 2013, 35(1): 45-54.
	LI Z Q, LI J X, ZHANG G F. Expression regulation of plant ascorbate peroxidase and its tolerance to abiotic stresses[J]. Hereditas, 2013, 35(1): 45-54. (in Chinese with English abstract)
[31]	林源秀, 顾欣昕, 汤浩茹. 植物谷胱甘肽还原酶的生物学特性及功能[J]. 中国生物化学与分子生物学报, 2013, 29(6): 534-542.
	LIN Y X, GU X X, TANG H R. Characteristics and biological functions of glutathione reductase in plants[J]. Chinese Journal of Biochemistry and Molecular Biology, 2013, 29(6): 534-542. (in Chinese with English abstract)
[32]	YOUSUF P Y, HAKEEM K U R, CHANDNA R, et al. Role of glutathione reductase in plant abiotic stress[M]// AHMADP, PRASADM. Abiotic Stress Responses in Plants. New York: Springer, 2012: 149-158.
[33]	钟霞飞. 镉胁迫对多年生黑麦草的生理影响及外源褪黑素的缓解效应[D]. 成都: 四川农业大学, 2018.
	ZHONG X F. The physiological effects of cadmium stress on Lolium perenne L. and effect of exogenous melatonin to mitigation[D]. Chengdu: Sichuan Agricultural University, 2018. (in Chinese with English abstract)
[34]	孙博. AtOxR-CSN5B相互作用正调控AsA含量缓解氧化胁迫机制[D]. 哈尔滨: 东北林业大学, 2014.
	SUN B. The novel protein AtOxR interacts with CSN5B to play A role in H₂O₂scavenging and increase AsA synthesis to lowers oxidative stress in Arabidopsis thaliana[D]. Harbin:Northeast Forestry University, 2014. (in Chinese with English abstract)
[35]	夏惠, 林玲, 高帆, 等. 甜樱桃‘佐藤锦’果实生长发育过程AsA含量及其相关酶活性的变化[J]. 西北植物学报, 2016, 36(10): 2008-2014.
	XIA H, LIN L, GAO F, et al. Changes of AsA content and related enzyme activities in sweet cherry‘Satonishiki’ fruit during development[J]. Acta Botanica Boreali-Occidentalia Sinica, 2016, 36(10): 2008-2014. (in Chinese with English abstract)
[36]	刘啸然. 苗期低氮处理对水培黄瓜生长及AsA代谢相关酶活性的影响[D]. 北京: 中国农业科学院, 2011.
	LIU X R. Effects of low nitrogen treatment during seedling stage on the growth and enzymes related to the metabolism of ascorbic acid of hydroponic cucumber[D]. Beijing: Chinese Academy of Agricultural Sciences, 2011. (in Chinese with English abstract)
[37]	杜昕, 李博, 毛鲁枭, 等. 褪黑素对干旱胁迫下大豆产量及AsA-GSH循环的影响[J]. 作物杂志, 2022(1): 174-178.
	DU X, LI B, MAO L X, et al. Effects of melatonin on yield and AsA-GSH cycle in soybean under drought stress[J]. Crops, 2022(1): 174-178. (in Chinese with English abstract)

Effects of melatonin seed priming on growth and physiological characteristics of Capsicum annuum under drought stress

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 37

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Yu, WANG Hong, XIAO Jiujun, LI Kexiang, XING Dan, ZHANG Yongliang, CHEN Yang, ZHANG Lanyue. Numerical estimation of chlorophyll in pepper leaves based on optimized vegetation index combination [J]. Acta Agriculturae Zhejiangensis, 2023, 35(9): 2109-2120.
[2]	MA Qiliang, YANG Xiaoming, HU Shuixing, HUANG Zihong, QI Hengnian. Automatic detection method of corn seed germination based on Mask RCNN and vision technology [J]. Acta Agriculturae Zhejiangensis, 2023, 35(8): 1927-1936.
[3]	SUN Xiujuan, XU Weihui, WANG Zhigang. Isolation and identification of endophytic bacteria from soybean nodule and their effects on soybean plant [J]. Acta Agriculturae Zhejiangensis, 2023, 35(7): 1532-1541.
[4]	HOU Caixia, DING Dedong, HE Jing, ZHAO Jitao, LI Yanxiang, ZHAO Qian, ZHANG Chongqing, LI Nan. Screening, identification and biocontrol effect of endophytic fungus from Lycium barbarum [J]. Acta Agriculturae Zhejiangensis, 2023, 35(7): 1662-1671.
[5]	ZHANG Xuenan, WANG Lele, NIU Mingxuan, ZHAN Ni, REN Haojie, XU Haocong, YANG Kun, WU Liquan, KE Jian, YOU Cuicui, HE Haibing. Estimation of rice leaf water content based on leaf reflectance spectrum and chlorophyll fluorescence [J]. Acta Agriculturae Zhejiangensis, 2023, 35(6): 1265-1277.
[6]	ZHU Yan, WEI Jia, XU Zilong, LIN Tianbao, YANG Sheng, LIU Yan, LYU Zhiqiang, LIU Peigang. Effects of growth promoting hormones on physiological and biochemical indexes of mulberry leaves during senescence [J]. Acta Agriculturae Zhejiangensis, 2023, 35(6): 1278-1285.
[7]	LUO Hailin, YUAN Lei, YAN Jiahui, GUO Qingyun. Cloning and identification of Pepper cryptic virus 2 in Qinghai Province, China [J]. Acta Agriculturae Zhejiangensis, 2023, 35(6): 1368-1374.
[8]	YANG Songhua, SHI Guiyang, WANG Jingqin, CHEN Zhu. Effects of soybean root exudates on insoluble phosphorus in soil under low phosphorus stress [J]. Acta Agriculturae Zhejiangensis, 2023, 35(6): 1396-1406.
[9]	CHAI Guanqun, ZHOU Wei, LIANG Hong, FAN Feifei, ZHU Dayan, FAN Chengwu. Effect of foliar spraying of zinc fertilizer and citric acid on yield, quality and Cd absorption and transport ation of pepper [J]. Acta Agriculturae Zhejiangensis, 2023, 35(5): 1069-1079.
[10]	XIA Xiaodong, ZHANG Xiaobo, SHI Yongfeng, XU Rugen. Research progress in gene cloning and molecular mechanism of rice lethal mutants [J]. Acta Agriculturae Zhejiangensis, 2023, 35(5): 1223-1234.
[11]	ZHU Li, XUE Xinru, LIANG Zongxu, XU Linyu, YU Guofeng, TANG Yi, LI Huanxiu. Correlation between capsaicin content and agronomic traits in pepper (Capsicum annuum L.) germplasm resources [J]. Acta Agriculturae Zhejiangensis, 2023, 35(4): 862-872.
[12]	HUANG Wanyuan, LI Caibin, PENG Yu, LI Zhanghai, HUANG Yanzhang, DING Ting. Studies on isolation and identification of antagonistic bacteria against tobacco root black rot pathogen, Thielaviopsis basicola and their biocontrol characteristics [J]. Acta Agriculturae Zhejiangensis, 2023, 35(4): 873-883.
[13]	ZHAO Shuhui, ZHANG Zhenhua, OU Zhangdan, TIAN Maoping, CHEN Yumei, ZHAO Ziwei. Preliminary analysis on research hotspot of crop root exudates in China [J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 534-546.
[14]	SUN Nan, YAN Guochao, HE Yong, ZHU Zhujun. Rapid determination of silicon content in Cucurbitaceae leaves by silicon molybdenum blue method [J]. Acta Agriculturae Zhejiangensis, 2023, 35(2): 338-345.
[15]	GENG Bingjie, YE Miaomiao, CHEN Yan, WANG Mengchang, MA Shangyu, HUANG Zhenglai, ZHANG Wenjing, FAN Yonghui. Effects of exogenous 6-BA and KH₂PO₄ on antioxidant enzymes and anaerobic respiratory enzymes activities in wheat roots under waterlogging at post-flowering stage [J]. Acta Agriculturae Zhejiangensis, 2023, 35(10): 2275-2285.