Effects of NaCl on rooting, in-situ distribution of IAA and related enzyme activities of cherry rootstock seedlings

doi:10.3969/j.issn.1004-1524.20230812

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (6): 1300-1308.DOI: 10.3969/j.issn.1004-1524.20230812

• Horticultural Science • Previous Articles Next Articles

Effects of NaCl on rooting, in-situ distribution of IAA and related enzyme activities of cherry rootstock seedlings

GAO Lanyun¹(), LIU Hao², LI Ai¹, ZHANG Tingting¹, YANG Lifang², GAO Ying¹^,^*()

1. College of Horticulture and Landscape Architecture, Tianjin Agricultural University, Tianjin 300392, China
2. Forestry Fruit Research Institute, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China

Received:2023-06-29 Online:2024-06-25 Published:2024-07-02

Abstract

Abstract:

In order to clarify the effect of NaCl on the rooting of cherry rootstock, this experiment took the Gisela 6 tissue culture seedling as the material, and firstly studied the index of adventitious root occurrence in the tissue culture seedling treated with different concentrations of NaCl. Then, the anatomical structure, enzyme activity and in-situ distribution of IAA under 10 mmol·L^-1and 80 mmol·L^-1NaCl were further studied according to rooting indexes. The results showed that the rooting rate and rooting index of cherry tissue seedlings were the highest under the treatment of 10 mmol·L^-1NaCl, and were significantly higher than other treatments, which were 58% and 13.43, respectively. Under this treatment the rooting speed was the fastest, the cambium thickened at 2 d, the root primodia formed at 6 d, and the adventitious roots broke through the epidermis at 10 d. SOD activity of each treatment reached the peak at 6 d. The activity changes of POD, PPO and IAAO were different among all treatments, while the activity of CK showed an increasing trend. Compared with CK, the activity of IAAO in 10 mmol·L^-1 treatment peaked at 2 d. Using immune colloidal gold technique to analysis the in-situ distribution of IAA, we found under 10 mmol·L^-1 NaCl treatment the IAA mainly distributed in cambium at 2 d, in root primordium at 6 d, and in the adventitious root vascular tissue at 10 d. In conclusion, 10 mmol·L^-1NaCl treatment can promote adventitious roots of cherry tissue culture seedlings, and provide a reference for further research on the occurrence and regulation mechanism of adventitious roots.

Key words: cherry, rootstock, adventitious rooting, enzymatic activity, immunohistochemical

CLC Number:

S662.5

GAO Lanyun, LIU Hao, LI Ai, ZHANG Tingting, YANG Lifang, GAO Ying. Effects of NaCl on rooting, in-situ distribution of IAA and related enzyme activities of cherry rootstock seedlings[J]. Acta Agriculturae Zhejiangensis, 2024, 36(6): 1300-1308.

Figures/Tables 5

Table 1 The rooting related parameters of cherry stem under NaCl treatment

NaCl浓度 NaCl concentration/ (mmol·L^-1)	生根率 Rooting rate/%	平均根数/条 Average number of roots	生根总数/条 Total number of roots	平均根长 Average length of roots/cm	最大根长 Maximum root length/cm	生根指数 Rooting index
CK(0)	40±3.07 b	2.68±0.24 a	37.33±4.16 a	4.36±0.09 ab	8.00±0.80 b	8.47±0.22 b
10	58±1.01 a	2.37±0.12 a	45.00±4.36 a	4.69±0.11 a	10.47±0.46 a	13.43±1.04a
20	41±2.67 b	2.63±0.09 a	37.67±4.51 a	4.20±0.34 b	7.83±0.76 b	8.39±0.84 b
40	25±3.08 c	2.75±0.65 a	23.33±4.16 b	3.78±0.06 c	6.73±0.40 b	4.46±0.52 c
80	5±4.32 d	2.44±0.96 a	3.33±0.58 c	2.43±0.29 d	3.40±0.79 c	0.32±0.03 d

Fig.1 The rooting rate of cherry stem under NaCl treatment

Fig.2 Anatomical structure of cherry stem during rooting under NaCl treatment a, b and c represent rooting induction under 0, 10 and 80 mmol·L-1 NaCl treatment for 2 days, respectively; d, e and f represent rooting induction for 6 days, respectively (same treatment as above); g, h, and i represent rooting induction for 10 days, respectively (same treatment as above); j represent rooting induction for 0 days; d(1) and e(1) represent the enlarged figure 10× of Figure d and e respectively. Ep, Epidermis; Co, Cortex; Ca, Cambium; Ph, Phloem; Xy, Xylem; Pi, Pith; Rp, Root primordium; Ar, Adventitious root.

Fig.3 Changes of enzyme activity during rooting of cherry stem segment under NaCl treatment The bars with different lowercase letters indicate the significant difference (P<0.05).

Fig.4 Changes of IAA distribution during cherry stem rooting a, b and c represent rooting induction under 0, 10 and 80 mmol·L-1NaCl treatment for 2 days respectively. d, e and f represent rooting induction for 6 days respectively (same treatment as above), and IAA was mainly distributed in cambium and phloem. e. IAA was mainly distributed in the root primordia. g, h, and i represent rooting induction for 10 days respectively (same treatment as above). IAA was mainly distributed in root tips with the 10 mmol·L-1 treatment. j, k, l represent the transverse slices of the stem base before rooting induction respectively. IAA was distributed in the cambium of Figure j; Figure k represents omitting the IAA monoclonal antibody; Figure l represents omitting the gold label secondary antibody.

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Effects of NaCl on rooting, in-situ distribution of IAA and related enzyme activities of cherry rootstock seedlings

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