Genetic diversity and association analysis between agronomic traits and SSR markers in hulless barley

doi:10.3969/j.issn.1004-1524.20231050

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (9): 1977-1989.DOI: 10.3969/j.issn.1004-1524.20231050

• Crop Seience • Previous Articles Next Articles

Genetic diversity and association analysis between agronomic traits and SSR markers in hulless barley

ZHANG Yuanyuan¹^,²(), FENG Juling¹^,², XIAO Jingfeng¹^,², GUAN Yu¹^,², LONG Chuer¹^,², YAO Lirong¹^,², MENG Yaxiong¹^,², SI Erjing¹^,², LI Baochun¹^,³, MA Xiaole¹^,², WANG Huajun¹^,², ZHOU Xirong⁴, LIU Meijin⁴, WANG Juncheng¹^,²^,^*()

1. State Key Laboratory of Aridland Crop Science/Gansu Provincial Key Laboratory of Crop Improvement & Germplasm Enhancement, Lanzhou 730070, China
2. College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China
3. College of Life Science and Techology, Gansu Agricultural University, Lanzhou 730070, China
4. Gannan Tibetan Autonomous Prefecture Agricultural Science Research Institute, Hezuo 747000, Gansu, China

Received:2023-09-02 Online:2024-09-25 Published:2024-09-30

Abstract

Abstract:

In order to understand the genetic diversity of hulless barley germplasms from different regions and to discover markers associated with agronomic traits. In this study, we analyzed the genetic diversity and population genetic structure of 102 hulless barley germplasms from different regions of Gansu and Xizang using 92 pairs of SSR markers, and the association analysis between SSR markers and agronomic traits was carried out by using the GLM model of Tassel 2.1 software. The results showed that a total of 739 alleles were identified by scanning 92 markers, and the variance of gene diversity index ranged from 0.106 to 0.913, the variance of polymorphism information content ranged from 0.103 to 0.907; the genetic similarity coefficient varied from 0.680 to 0.960, and the clustering analysis divided these hulless barley germplasms into two subgroups, germplasms with the same parentage or similar kinship were clustered together; the population genetic structure analysis divided the hulless barley germplasms into five subgroups; it was revealed that more than 9 agronomic traits of 33 markers were extremely remarkalbe at the P<0.01 level, there were 10 markers associated with thousand grain weight, of which the marker GBM1238 was more stable, and the explained rate of the phenotypic variance of these markers ranged from 8.95% to 35.02%. In conclusion, most of the hulless barley germplasms for the test in this study are similarly related, and different hulless barley genetic resources can be introduced to enrich the genetic background.

Key words: hulless barley, SSR, agronomic trait, genetic diversity, association analysis

CLC Number:

S512.3

ZHANG Yuanyuan, FENG Juling, XIAO Jingfeng, GUAN Yu, LONG Chuer, YAO Lirong, MENG Yaxiong, SI Erjing, LI Baochun, MA Xiaole, WANG Huajun, ZHOU Xirong, LIU Meijin, WANG Juncheng. Genetic diversity and association analysis between agronomic traits and SSR markers in hulless barley[J]. Acta Agriculturae Zhejiangensis, 2024, 36(9): 1977-1989.

Figures/Tables 7

References 37

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编号 No.	名称 Name	来源 Origin place	编号 No.	名称 Name	来源 Origin place
1	长芒青稞Changmangqingke	甘南临潭Lintan, Gannan	52	青稞4号Qingke 4	天水张川Zhangchuan, Tianshui
2	碌曲白青稞Luqubaiqingke	甘南碌曲Luqu, Gannan	53	青稞5号Qingke 5	临夏永靖Yongjing, Linxia
3	碌曲紫青稞Luquziqingke	甘南碌曲Luqu, Gannan	54	青稞6号Qingke 6	酒泉安西Anxi, Jiuquan
4	土房紫青稞Tufangziqingke	甘南夏河Xiahe, Gannan	55	青稞7号Qingke 7	天水武山Wushan, Tianshui
5	舟曲春青稞Zhouquchunqingke	甘南舟曲Zhouqu, Gannan	56	青稞8号Qingke 8	天水清水Qingshui, Tianshui
6	宕昌蓝青稞Dangchanglanqingke	陇南宕昌Dangchang, Longnan	57	青溜子Qingliuzi	甘南临潭Lintan, Gannan
7	宕昌黄青稞Dangchanghuangqingke	陇南宕昌Dangchang, Longnan	58	和尚青稞Heshangqingke	临夏积石山Jishishan, Linxia
8	岷县青稞Minxianqingke	定西岷县Minxian, Dingxi	59	卷芒青稞Juanmangqingke	武威古浪Gulang, Wuwei
9	漳县青稞Zhangxianqingke	定西漳县Zhangxian, Dingxi	60	春青稞Chunqingke	陇南礼县Lixian, Longnan
10	漳县长芒青稞Zhangxianchangmangqingke	定西漳县Zhangxian, Dingxi	61	绿青稞Lvqingke	临夏和政Hezheng, Linxia
11	欧娃青稞Ouwaqingke	定西岷县Minxian, Dingxi	62	麻青稞1号Maqingke 1	临夏和政Hezheng, Linxia
12	青稞1号Qingke 1	甘南夏河Xiahe, Gannan	63	麻青稞2号Maqingke 2	甘南夏河Xiahe, Gannan
13	紫青稞1号Ziqingke 1	甘南夏河Xiahe, Gannan	64	紫青稞2号Ziqingke 2	张掖民乐Minle, Zhangye
14	土葫芦青稞Tuhuluqingke	金昌永昌Yongchang, Jinchang	65	紫青稞3号Ziqingke 3	甘南卓尼Zhuoni, Gannan
15	大青稞Daqingke	临夏Linxia	66	紫青稞4号Ziqingke 4	武威Wuwei
16	大麻青稞Damaqingke	定西临洮Lintao, Dingxi	67	紫青稞5号Ziqingke 5	酒泉金塔Jinta, Jiuquan
17	小青稞Xiaoqingke	临夏Linxia	68	紫青稞6号Ziqingke 6	甘南夏河Xiahe, Gannan
18	无叶耳青稞Wuyeerqingke	甘南Gannan	69	紫青稞7号Ziqingke 7	甘南Gannan
19	无叶耳紫青稞Wuyeerziqingke	甘南夏河Xiahe, Gannan	70	紫眼窝青稞Ziyanwoqingke	张掖Zhangye
20	无皮青稞Wupiqingke	陇南礼县Lixian, Longnan	71	锉青稞Cuoqingke	甘南临潭Lintan, Gannan
21	无芒青稞Wumangqingke	平凉Pingliang	72	青稞9号Qingke 9	张掖民乐Minle, Zhangye
22	长青稞Changqingke	甘南临潭Lintan, Gannan	73	蓝青稞1号Lanqingke 1	张掖民乐Minle, Zhangye
23	六棱青稞Liulengqingke	张掖临泽Linze, Zhangye	74	蓝青稞2号Lanqingke 2	张掖民乐Minle, Zhangye
24	六棱小青稞Liulengxiaoqingke	张掖临泽Linze, Zhangye	75	蓝青稞3号Lanqingke 3	陇南宕昌Dangchang, Longnan
25	六棱头青稞Liulengtouqingke	酒泉金塔Jinta, Jiuquan	76	蓝青稞4号Lanqingke 4	甘南Gannan
26	六棱大青稞Liulengdaqingke	酒泉金塔Jinta, Jiuquan	77	褐青稞Heqingke	白银景泰Jingtai, Baiyin
27	六棱头紫青稞Liulengtouziqingke	张掖民乐Minle, Zhangye	78	藏青稞Zangqingke	张掖民乐Minle, Zhangye
28	六棱青稞1号Liulengqingke 1	武威民勤Minqin, Wuwei	79	六棱长芒黑青稞Liulengchangmangheiqingke	武威Wuwei
29	六棱青稞2号Liulengqingke 2	甘南舟曲Zhouqu, Gannan	80	藏青稞Zangqingke	武威Wuwei
30	六棱青稞3号Liulengqingke 3	甘南舟曲Zhouqu, Gannan	81	香立哈青稞Xianglihaqingke	西藏Xizang
31	六棱黑青稞Liulengheiqingke	武威Wuwei	82	青稞10号Qingke 10	西藏Xizang
32	白青稞1号Baiqingke 1	甘南夏河Xiahe, Gannan	83	马甲青稞Majiaqingke	西藏Xizang
33	白青稞2号Baiqingke 2	张掖肃南Sunan, Zhangye	84	农仁青稞Nongrenqingke	西藏Xizang
34	白青稞3号Baiqingke 3	武威Wuwei	85	青稞11号Qingke 11	兰州永登Yongdeng, Lanzhou
35	白青稞4号Baiqingke 4	白银景泰Jingtai, Baiyin	86	青稞12号Qingke 12	武威古浪Gulang, Wuwei
36	白青稞5号Baiqingke 5	白银景泰Jingtai, Baiyin	87	喜拉3号Xila 3	西藏Xizang
37	白青稞6号Baiqingke 6	武威古浪Gulang, Wuwei	88	喜拉4号Xila 4	西藏Xizang
38	白籽青稞Baiziqingke	临夏Linxia	89	喜拉5号Xila 5	西藏Xizang
39	老青稞1号Laoqingke 1	定西漳县Zhangxian, Dingxi	90	喜拉6号Xila 6	西藏Xizang
40	老青稞2号Laoqingke 2	天水甘谷Gangu, Tianshui	91	喜拉7号Xila 7	西藏Xizang
41	老青稞3号Laoqingke 3	临夏永靖Yongjing, Linxia	92	喜拉11号Xila 11	西藏Xizang
42	老青稞4号Laoqingke 4	陇南西和Xihe, Longnan	93	喜拉12号Xila 12	西藏Xizang
43	红青稞Hongqingke	甘南夏河Xiahe, Gannan	94	喜拉13号Xila 13	西藏Xizang
44	兴隆青稞Xinglongqingke	兰州榆中Yuzhong, Lanzhou	95	喜拉19号Xila 19	西藏Xizang
45	杈青稞Chaqingke	临夏Linxia	96	喜拉22号Xila 22	西藏Xizang
46	牡丹青稞Mudanqingke	临夏Linxia	97	喜拉42号Xila 42	西藏Xizang
47	青皮青稞1号Qingpiqingke 1	张掖河西Hexi, Zhangye	98	藏青1号Zangqing 1	西藏Xizang
48	青皮青稞2号Qingpiqingke 2	张掖Zhangye	99	藏青2号Zangqing 2	西藏Xizang
49	青青稞Qingqingke	兰州永登Yongdeng, Lanzhou	100	藏青280号Zangqing 280	西藏Xizang
50	青稞2号Qingke 2	兰州榆中Yuzhong, Lanzhou	101	藏青690号Zangqing 690	西藏Xizang
51	青稞3号Qingke 3	天水清水Qingshui, Tianshui	102	高厚旱1号Gaohouhan 1	西藏Xizang

编号 No.	名称 Name	来源 Origin place	编号 No.	名称 Name	来源 Origin place
1	长芒青稞Changmangqingke	甘南临潭Lintan, Gannan	52	青稞4号Qingke 4	天水张川Zhangchuan, Tianshui
2	碌曲白青稞Luqubaiqingke	甘南碌曲Luqu, Gannan	53	青稞5号Qingke 5	临夏永靖Yongjing, Linxia
3	碌曲紫青稞Luquziqingke	甘南碌曲Luqu, Gannan	54	青稞6号Qingke 6	酒泉安西Anxi, Jiuquan
4	土房紫青稞Tufangziqingke	甘南夏河Xiahe, Gannan	55	青稞7号Qingke 7	天水武山Wushan, Tianshui
5	舟曲春青稞Zhouquchunqingke	甘南舟曲Zhouqu, Gannan	56	青稞8号Qingke 8	天水清水Qingshui, Tianshui
6	宕昌蓝青稞Dangchanglanqingke	陇南宕昌Dangchang, Longnan	57	青溜子Qingliuzi	甘南临潭Lintan, Gannan
7	宕昌黄青稞Dangchanghuangqingke	陇南宕昌Dangchang, Longnan	58	和尚青稞Heshangqingke	临夏积石山Jishishan, Linxia
8	岷县青稞Minxianqingke	定西岷县Minxian, Dingxi	59	卷芒青稞Juanmangqingke	武威古浪Gulang, Wuwei
9	漳县青稞Zhangxianqingke	定西漳县Zhangxian, Dingxi	60	春青稞Chunqingke	陇南礼县Lixian, Longnan
10	漳县长芒青稞Zhangxianchangmangqingke	定西漳县Zhangxian, Dingxi	61	绿青稞Lvqingke	临夏和政Hezheng, Linxia
11	欧娃青稞Ouwaqingke	定西岷县Minxian, Dingxi	62	麻青稞1号Maqingke 1	临夏和政Hezheng, Linxia
12	青稞1号Qingke 1	甘南夏河Xiahe, Gannan	63	麻青稞2号Maqingke 2	甘南夏河Xiahe, Gannan
13	紫青稞1号Ziqingke 1	甘南夏河Xiahe, Gannan	64	紫青稞2号Ziqingke 2	张掖民乐Minle, Zhangye
14	土葫芦青稞Tuhuluqingke	金昌永昌Yongchang, Jinchang	65	紫青稞3号Ziqingke 3	甘南卓尼Zhuoni, Gannan
15	大青稞Daqingke	临夏Linxia	66	紫青稞4号Ziqingke 4	武威Wuwei
16	大麻青稞Damaqingke	定西临洮Lintao, Dingxi	67	紫青稞5号Ziqingke 5	酒泉金塔Jinta, Jiuquan
17	小青稞Xiaoqingke	临夏Linxia	68	紫青稞6号Ziqingke 6	甘南夏河Xiahe, Gannan
18	无叶耳青稞Wuyeerqingke	甘南Gannan	69	紫青稞7号Ziqingke 7	甘南Gannan
19	无叶耳紫青稞Wuyeerziqingke	甘南夏河Xiahe, Gannan	70	紫眼窝青稞Ziyanwoqingke	张掖Zhangye
20	无皮青稞Wupiqingke	陇南礼县Lixian, Longnan	71	锉青稞Cuoqingke	甘南临潭Lintan, Gannan
21	无芒青稞Wumangqingke	平凉Pingliang	72	青稞9号Qingke 9	张掖民乐Minle, Zhangye
22	长青稞Changqingke	甘南临潭Lintan, Gannan	73	蓝青稞1号Lanqingke 1	张掖民乐Minle, Zhangye
23	六棱青稞Liulengqingke	张掖临泽Linze, Zhangye	74	蓝青稞2号Lanqingke 2	张掖民乐Minle, Zhangye
24	六棱小青稞Liulengxiaoqingke	张掖临泽Linze, Zhangye	75	蓝青稞3号Lanqingke 3	陇南宕昌Dangchang, Longnan
25	六棱头青稞Liulengtouqingke	酒泉金塔Jinta, Jiuquan	76	蓝青稞4号Lanqingke 4	甘南Gannan
26	六棱大青稞Liulengdaqingke	酒泉金塔Jinta, Jiuquan	77	褐青稞Heqingke	白银景泰Jingtai, Baiyin
27	六棱头紫青稞Liulengtouziqingke	张掖民乐Minle, Zhangye	78	藏青稞Zangqingke	张掖民乐Minle, Zhangye
28	六棱青稞1号Liulengqingke 1	武威民勤Minqin, Wuwei	79	六棱长芒黑青稞Liulengchangmangheiqingke	武威Wuwei
29	六棱青稞2号Liulengqingke 2	甘南舟曲Zhouqu, Gannan	80	藏青稞Zangqingke	武威Wuwei
30	六棱青稞3号Liulengqingke 3	甘南舟曲Zhouqu, Gannan	81	香立哈青稞Xianglihaqingke	西藏Xizang
31	六棱黑青稞Liulengheiqingke	武威Wuwei	82	青稞10号Qingke 10	西藏Xizang
32	白青稞1号Baiqingke 1	甘南夏河Xiahe, Gannan	83	马甲青稞Majiaqingke	西藏Xizang
33	白青稞2号Baiqingke 2	张掖肃南Sunan, Zhangye	84	农仁青稞Nongrenqingke	西藏Xizang
34	白青稞3号Baiqingke 3	武威Wuwei	85	青稞11号Qingke 11	兰州永登Yongdeng, Lanzhou
35	白青稞4号Baiqingke 4	白银景泰Jingtai, Baiyin	86	青稞12号Qingke 12	武威古浪Gulang, Wuwei
36	白青稞5号Baiqingke 5	白银景泰Jingtai, Baiyin	87	喜拉3号Xila 3	西藏Xizang
37	白青稞6号Baiqingke 6	武威古浪Gulang, Wuwei	88	喜拉4号Xila 4	西藏Xizang
38	白籽青稞Baiziqingke	临夏Linxia	89	喜拉5号Xila 5	西藏Xizang
39	老青稞1号Laoqingke 1	定西漳县Zhangxian, Dingxi	90	喜拉6号Xila 6	西藏Xizang
40	老青稞2号Laoqingke 2	天水甘谷Gangu, Tianshui	91	喜拉7号Xila 7	西藏Xizang
41	老青稞3号Laoqingke 3	临夏永靖Yongjing, Linxia	92	喜拉11号Xila 11	西藏Xizang
42	老青稞4号Laoqingke 4	陇南西和Xihe, Longnan	93	喜拉12号Xila 12	西藏Xizang
43	红青稞Hongqingke	甘南夏河Xiahe, Gannan	94	喜拉13号Xila 13	西藏Xizang
44	兴隆青稞Xinglongqingke	兰州榆中Yuzhong, Lanzhou	95	喜拉19号Xila 19	西藏Xizang
45	杈青稞Chaqingke	临夏Linxia	96	喜拉22号Xila 22	西藏Xizang
46	牡丹青稞Mudanqingke	临夏Linxia	97	喜拉42号Xila 42	西藏Xizang
47	青皮青稞1号Qingpiqingke 1	张掖河西Hexi, Zhangye	98	藏青1号Zangqing 1	西藏Xizang
48	青皮青稞2号Qingpiqingke 2	张掖Zhangye	99	藏青2号Zangqing 2	西藏Xizang
49	青青稞Qingqingke	兰州永登Yongdeng, Lanzhou	100	藏青280号Zangqing 280	西藏Xizang
50	青稞2号Qingke 2	兰州榆中Yuzhong, Lanzhou	101	藏青690号Zangqing 690	西藏Xizang
51	青稞3号Qingke 3	天水清水Qingshui, Tianshui	102	高厚旱1号Gaohouhan 1	西藏Xizang

农艺性状 Agronomic trait	最大值 Maximum value	最小值 Minimum value	平均值±标准误 Average value±standard error	变异系数 Coefficient of variation
株高Plant height/cm	120.00	45.40	98.65±9.90	0.10
穗长Spike length/cm	8.90	3.60	6.29±1.06	0.17
单株分蘖数/个Number of tillers per plant	2.00	0.10	0.67±0.30	0.44
单株粒数/粒Number of grains per plant	111.00	37.00	70.60±15.30	0.22
单株粒重Grain weight per plant/g	5.50	2.00	3.47±0.80	0.23
穗粒数/粒Number of grains per spike	68.00	18.00	42.81±9.50	0.22
穗粒重Grain weight per spike/g	3.60	1.10	2.13±0.50	0.25
千粒重Thousand grain weight/g	64.40	33.30	49.60±5.60	0.11
生育期Growth period/d	128.00	116.00	119.54±1.85	0.02

农艺性状 Agronomic trait	最大值 Maximum value	最小值 Minimum value	平均值±标准误 Average value±standard error	变异系数 Coefficient of variation
株高Plant height/cm	120.00	45.40	98.65±9.90	0.10
穗长Spike length/cm	8.90	3.60	6.29±1.06	0.17
单株分蘖数/个Number of tillers per plant	2.00	0.10	0.67±0.30	0.44
单株粒数/粒Number of grains per plant	111.00	37.00	70.60±15.30	0.22
单株粒重Grain weight per plant/g	5.50	2.00	3.47±0.80	0.23
穗粒数/粒Number of grains per spike	68.00	18.00	42.81±9.50	0.22
穗粒重Grain weight per spike/g	3.60	1.10	2.13±0.50	0.25
千粒重Thousand grain weight/g	64.40	33.30	49.60±5.60	0.11
生育期Growth period/d	128.00	116.00	119.54±1.85	0.02

标记 Marker	染色体 Chromosome	遗传位置 Genetic location	等位基因数 Number of alleles	基因型数 Number of genotypes	主等位基因频率 Main allele frequency	基因多样性指数 Genetic diversity index	多态信息含量 Polymorphic information content
GMS021	1H	25.62	8	12	0.315	0.784	0.754
BMAC0399	1H	28.86	10	12	0.241	0.838	0.818
BMAG0872	1H	49.78	19	11	0.181	0.897	0.888
BMAG0347	1H	53.27	12	13	0.343	0.747	0.710
BMAG0345	1H	58.64	9	6	0.347	0.741	0.702
GBM1487	1H	60.42	4	3	0.495	0.522	0.408
BMAC0032	1H	73.34	12	11	0.431	0.684	0.633
SCSSR10477	1H	79.09	8	5	0.486	0.536	0.428
BMAC0154	1H	87.83	13	13	0.384	0.768	0.739
GBM1143	1H	92.93	7	8	0.477	0.651	0.593
GMS149	1H	129.52	8	8	0.463	0.601	0.520
SCSSR02748	1H	133.46	6	8	0.329	0.768	0.732
GBMS002	2H	37.94	6	4	0.398	0.665	0.603
GBM1446	2H	58.13	6	4	0.449	0.605	0.525
GBM1251	3H	58.80	5	4	0.370	0.745	0.706
SCSSR03381	2H	63.61	7	5	0.384	0.704	0.654
GBM1459	2H	64.35	6	5	0.407	0.686	0.630
GBMS160	2H	75.31	7	5	0.398	0.687	0.634
BMAG0381	2H	76.22	8	9	0.343	0.751	0.712
EBMATC0039	2H	93.26	8	6	0.481	0.636	0.572
GBM1440	2H	96.34	6	6	0.472	0.561	0.464
GBM1309	2H	107.18	6	4	0.444	0.682	0.629
GBM1149	2H	107.89	10	7	0.394	0.747	0.712
HVM54	2H	122.41	9	10	0.412	0.752	0.722
GBM1498	2H	125.71	10	7	0.319	0.802	0.777
BMAG0749	2H	149.73	5	4	0.431	0.642	0.574
MWG2200	2H	154.42	6	11	0.495	0.696	0.665
GBM1078	3H	15.39	7	5	0.463	0.615	0.539
EBMAC0705	3H	20.49	6	5	0.338	0.731	0.685
GBM1413	3H	49.69	8	6	0.440	0.627	0.556
BMAG0006	3H	50.14	4	2	0.454	0.584	0.496
BMAG0131	3H	51.06	10	8	0.269	0.795	0.764
GBM1444	3H	51.46	8	8	0.477	0.653	0.592
GBM1139	3H	52.02	11	10	0.458	0.589	0.503
GBM1242	3H	55.43	6	4	0.481	0.545	0.441
GBM1110	3H	60.27	5	4	0.440	0.633	0.561
SCSSR25691	3H	68.16	6	4	0.412	0.656	0.593
HVM60	3H	73.19	9	6	0.227	0.846	0.828
BMAG0841	3H	91.21	6	7	0.838	0.290	0.279
ABG004	3H	111.42	6	8	0.671	0.497	0.449
BMAC0144K	3H	136.17	6	6	0.407	0.694	0.641
EBMAC0541	3H	137.16	8	6	0.306	0.788	0.758
HVM62	3H	139.43	6	6	0.579	0.539	0.454
EBMAC0708	3H	142.33	9	12	0.347	0.751	0.714
GBM1238	3H	146.66	8	5	0.440	0.616	0.540
GBM1420	3H	152.53	3	4	0.514	0.508	0.388
GBMS183	3H	154.36	6	6	0.491	0.640	0.579
MWG2282	4H	5.96	5	8	0.838	0.289	0.276
GBM1465	4H	17.06	5	6	0.880	0.222	0.215
HVM40	4H	22.40	6	6	0.347	0.740	0.697
GBM1252	4H	27.35	10	12	0.449	0.614	0.539
GBM1323	4H	28.96	8	6	0.463	0.589	0.504
BMAG0740	4H	50.86	16	23	0.264	0.847	0.830
EBMAG0781	4H	57.42	5	3	0.250	0.755	0.709
BMAC0030	4H	58.60	15	14	0.366	0.722	0.679
BMAG0490	4H	62.19	5	5	0.491	0.581	0.494
HVM68	4H	62.52	7	5	0.440	0.615	0.538
BMAC0310	4H	64.37	11	11	0.384	0.713	0.667
BMAG0353	4H	65.01	4	3	0.486	0.625	0.554
SCSSR14079	4H	78.92	7	4	0.384	0.690	0.638
HVM67	4H	120.5	6	5	0.468	0.613	0.537
SCSSR07106	5H	20.44	6	4	0.449	0.593	0.509
BMAC0163	5H	37.74	9	5	0.241	0.772	0.733
EBMAC0970	5H	40.77	5	3	0.366	0.701	0.648
BMAG0571	5H	42.87	7	9	0.435	0.662	0.603
GBM1293	5H	50.85	6	4	0.417	0.652	0.589
BMAG0760	5H	68.72	8	9	0.264	0.813	0.788
GBM1399	5H	69.86	2	3	0.657	0.450	0.349
GBM1426	5H	70.25	5	5	0.528	0.528	0.420
SCSSR16991	5H	76.81	4	5	0.944	0.106	0.103
SCSSR15334	5H	77.79	11	12	0.343	0.756	0.717
BMAC0144L	5H	84.78	10	9	0.389	0.702	0.652
BMAG0223	5H	86.88	23	15	0.167	0.913	0.907
BMAG0812	5H	90.32	13	14	0.347	0.743	0.703
SCSSR05939	5H	90.64	4	5	0.708	0.449	0.400
GBM1141	5H	108.26	4	7	0.644	0.498	0.424
HVDHN09	5H	110.11	8	8	0.477	0.581	0.493
GMS061	5H	122.09	11	9	0.394	0.722	0.681
EBMAC0824	5H	125.75	5	7	0.759	0.403	0.379
SCSSR02306	5H	6.13	8	9	0.444	0.656	0.596
GBM1176	5H	18.59	5	5	0.500	0.628	0.565
BMAC0113	5H	53.12	15	9	0.222	0.858	0.843
SCSSR15335	5H	77.79	6	8	0.676	0.480	0.423
BMAG0113F	5H	84.78	14	8	0.157	0.891	0.881
BMAG0113D	5H	91.82	17	9	0.134	0.909	0.902
SCSSR10148	5H	122.34	11	10	0.398	0.708	0.663
BMAG0113I	5H	160	10	8	0.236	0.817	0.791
EBMAC0806	6H	75.46	9	8	0.574	0.576	0.513
GBM1275	6H	124.29	2	3	0.523	0.499	0.374
EBMAC0713	7H	12.35	15	21	0.190	0.867	0.853
HVM04	7H	22.19	9	10	0.421	0.721	0.680
SCSSR07970	7H	53.98	8	10	0.819	0.312	0.290
平均值Mean			8.033	7.446	0.436	0.653	0.600

Genetic diversity and association analysis between agronomic traits and SSR markers in hulless barley

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标记 Marker	株高 Plant height	穗长 Spike length	单株分蘖数 Number of tillers per plant	单株粒数 Number of grains per plant	单株粒重 Grain weight per plant	穗粒数 Number of grains per spike	穗粒重 Grain weight per spike	千粒重 Thousand grain weight	生育期 Growth period
BMAC0032									21.68
GBM1446		12.89
GBM1251		12.93
GBM1440						14.58	12.82
GBM1078									12.00
GBM1413								16.52
GBM1444		14.66						15.28
GBM1139								23.34	18.68
GBM1110		15.59
SCSSR25691				10.32
HVM60					14.13
EBMAC0541	21.23
HVM62								15.33
GBM1238								9.65
GBM1252				14.33		13.30
EBMAG0781		8.95
BMAC0030									22.85
BMAG0490		22.41						17.12
BMAC0310								13.68
SCSSR07106				19.53	14.92	19.37
BMAG0571		9.39
BMAG0760	10.14
GBM1399			25.1						35.02
GBM1426				17.34				21.11
SCSSR15334					20.85
HVDHN09			23.91	21.13	20.78
GMS061				24.43	24.28	26.20
EBMAC0824								16.27
SCSSR15335			24.21						22.33
SCSSR10148			23.07					17.39
BMAG0113I									29.88
EBMAC0713							30.97
HVM04					15.95

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