Research progress on resistance of pepper to Tomato spotted wilt virus (TSWV)

doi:10.3969/j.issn.1004-1524.20231156

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (10): 2416-2425.DOI: 10.3969/j.issn.1004-1524.20231156

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Research progress on resistance of pepper to Tomato spotted wilt virus (TSWV)

GUO Nana(), LI Wei, HUANG Lijuan, ZHANG Tao, WEI Bingqiang^*()

College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China

Received:2023-10-07 Online:2024-10-25 Published:2024-10-30

Abstract

Abstract:

Tospovirus, the only genus of viruses within the Bunyaviridae family that can invade plants, poses a significant challenge to global agriculture. Its primary member, Tomato spotted wilt virus (TSWV), is particularly detrimental to the pepper industry. TSWV, a negative single-stranded RNA virus, is primarily transmitted through the persistent proliferation of thrips. Peppers frequently exhibit a range of complex symptoms, including leaf shedding, yellowing of new leaves, and necrosis at the growth point when infected with TSWV. In recent years, as bioinformatics research has advanced and the whole-genome sequencing of pepper has been completed, researchers have made significant progress in understanding the pathogenesis of TSWV and the mechanisms of resistance to TSWV in pepper plants. In terms of TSWV detection, PCR and RT-PCR technologies have been widely used for the precise detection of TSWV, especially the specific primers designed for the nuclear capsid protein gene (N-gene), which have significantly improved the detection efficiency and accuracy. Concurrently, molecular markers associated with resistance have been extensively applied for detecting and characterizing germplasm resources resistant to TSWV. The most effective strategy for preventing and controlling TSWV is to develop disease-resistant varieties. New varieties of resistant to TSWV in pepper such as ‘Zhongjiao 115’ have been successfully cultivated via extensive screening of resistant germplasm resources at home and abroad, combined with molecular marker technology. In addition, the virus can effectively evade the plant defense system through immune evasion strategies, such as antigenic structural mutations or host immunosuppression, thereby increasing the challenge of prevention and control. So, future research needs to focus on the in-depth mining of resistance genetic resources, improvement of breeding technology system and discovery of novel resistance genes, in order to breed pepper varieties with broader and more durable resistance.

Key words: pepper, Tomato spotted wilt virus, germplasm innovation, resistance

CLC Number:

S436.418.12

GUO Nana, LI Wei, HUANG Lijuan, ZHANG Tao, WEI Bingqiang. Research progress on resistance of pepper to Tomato spotted wilt virus (TSWV)[J]. Acta Agriculturae Zhejiangensis, 2024, 36(10): 2416-2425.

Figures/Tables 2

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引物名称 Primer name	正向引物序列 Forward primer sequence (5’→3’)	反向引物序列 Reverse primer sequence(5’→3’)	片段大小 Fragment size/bp	参考文献 Literature
TSWV cp	ATGTCTAAGGTTAAGCTCAC	TCAAGCAAGTTCTGCGAGTT	777	[37]
TSNSs	GGCTGTAGCAGAGAGCAATTGTGTCATAATTTT	GGACATAGCAAGATTATTTTGATCCTG	1 404	[38]
TSWV	TTAAGCAAGTTCTGTGAG	ATGTCTAAGGTTAAGCTC	774	[39]
TSWV	ATGTYTAAGGTTAAGCTCACTAAG	TTAAGCAAGTTCTGTGAGTTTTGCC	750	[40]
TSWV cp	TCACTGTAATGTTCCATAGCAA	AGAGCAATTGTGTCATTTTATTC	839	[41]
TSWV-N	AATTTCTCCGCAATCTATTTCAGTTG	GGGGATCCAGAGCAATTGTGTCAATTTT	1 720	[42]
TSNSs	CCGTCGACATGTCTTCAAGTGTT	GGCGGCCGCTTATTTTGATCCTGAA	—	[43]
TSWV	CGCCCTAAGAAACGACTG	CAACAAGCCTGACCCTGA	406	[44]
TSWV	GCCATGGTCTTCTTCTGATGAA	AGTTATTGTCCCCTGACCCTTC	451	[45]
TSNSs	ATGTCTTCAAGTGTTTATGAGTC	TTATTTTGATCCTGAAGCATACG	1 401	[45]
TSWV	CCCAGCATTATGGCAAGCC	GGGCTAGCGGAAAACCTCGACCAGATCA	350	[46]
TSWV	TTAAGCAAGTTCTGTGAG	ATGTCTAAGGTTAAGCTC	774	[47]
TSWV cp	ATGTCTAAGGTTAAGCTCACTA	AGCAAGTTCTGCGAGTTTTGC	777	[47]
TSWV-J	GTTGTACTTTCGCACCT	GCATACTCTTTCCCTTTC	—	[48]
TSWV-N	TTAAGCAAGTTCTGCAAGTT	CATGTCTAAGGTTAAGCTCAC	777	[49]

引物名称 Primer name	正向引物序列 Forward primer sequence (5’→3’)	反向引物序列 Reverse primer sequence(5’→3’)	片段大小 Fragment size/bp	参考文献 Literature
TSWV cp	ATGTCTAAGGTTAAGCTCAC	TCAAGCAAGTTCTGCGAGTT	777	[37]
TSNSs	GGCTGTAGCAGAGAGCAATTGTGTCATAATTTT	GGACATAGCAAGATTATTTTGATCCTG	1 404	[38]
TSWV	TTAAGCAAGTTCTGTGAG	ATGTCTAAGGTTAAGCTC	774	[39]
TSWV	ATGTYTAAGGTTAAGCTCACTAAG	TTAAGCAAGTTCTGTGAGTTTTGCC	750	[40]
TSWV cp	TCACTGTAATGTTCCATAGCAA	AGAGCAATTGTGTCATTTTATTC	839	[41]
TSWV-N	AATTTCTCCGCAATCTATTTCAGTTG	GGGGATCCAGAGCAATTGTGTCAATTTT	1 720	[42]
TSNSs	CCGTCGACATGTCTTCAAGTGTT	GGCGGCCGCTTATTTTGATCCTGAA	—	[43]
TSWV	CGCCCTAAGAAACGACTG	CAACAAGCCTGACCCTGA	406	[44]
TSWV	GCCATGGTCTTCTTCTGATGAA	AGTTATTGTCCCCTGACCCTTC	451	[45]
TSNSs	ATGTCTTCAAGTGTTTATGAGTC	TTATTTTGATCCTGAAGCATACG	1 401	[45]
TSWV	CCCAGCATTATGGCAAGCC	GGGCTAGCGGAAAACCTCGACCAGATCA	350	[46]
TSWV	TTAAGCAAGTTCTGTGAG	ATGTCTAAGGTTAAGCTC	774	[47]
TSWV cp	ATGTCTAAGGTTAAGCTCACTA	AGCAAGTTCTGCGAGTTTTGC	777	[47]
TSWV-J	GTTGTACTTTCGCACCT	GCATACTCTTTCCCTTTC	—	[48]
TSWV-N	TTAAGCAAGTTCTGCAAGTT	CATGTCTAAGGTTAAGCTCAC	777	[49]

基因型 Genotype	连锁标记类型 Marker type	标记名称 Name	参考文献 Literature
Tsw	CAPS	SCAC₅₆₈	[58]
BC₄F₁	RAPD	Q-06₂₇₀	[58]
‘SP’	SNP	SNP246056-1	[59]
‘Telmo’F₂		SNP246056-2
		SNP246056-3
		SNP246056-4
		SNP653492-1
		SNP653492-2
		SNP653492-3
		2465-1
		1072-1
		7715-1
		17918-1
PI 195301×	RAPD	OPAC10	[60]
PI152225 F₂		OPAH13
		OPAF16
		OPB01
	CAPS	SCAC568	[60]

基因型 Genotype	连锁标记类型 Marker type	标记名称 Name	参考文献 Literature
Tsw	CAPS	SCAC₅₆₈	[58]
BC₄F₁	RAPD	Q-06₂₇₀	[58]
‘SP’	SNP	SNP246056-1	[59]
‘Telmo’F₂		SNP246056-2
		SNP246056-3
		SNP246056-4
		SNP653492-1
		SNP653492-2
		SNP653492-3
		2465-1
		1072-1
		7715-1
		17918-1
PI 195301×	RAPD	OPAC10	[60]
PI152225 F₂		OPAH13
		OPAF16
		OPB01
	CAPS	SCAC568	[60]

Research progress on resistance of pepper to Tomato spotted wilt virus (TSWV)

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