Study on the residue elimination pattern of enrofloxacin and its metabolites in Jiangshan black-bone chickens

doi:10.3969/j.issn.1004-1524.20250673

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (4): 781-787.DOI: 10.3969/j.issn.1004-1524.20250673

• Quality and Safety of Agricultural Products • Previous Articles Next Articles

Study on the residue elimination pattern of enrofloxacin and its metabolites in Jiangshan black-bone chickens

QIAN Lingling¹(), QIAN Mingrong², LIN Hui³, LI Rui¹, ZHANG Hu¹, YU Ziqing¹, WANG Jianmei¹^,^*()

¹ Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
² Interdisciplinary Research Academy, Zhejiang Shuren University, Hangzhou 310015, China
³ Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2025-11-03 Online:2026-04-25 Published:2026-05-08
Contact: WANG Jianmei

Abstract

Abstract:

To study the residue elimination pattern of enrofloxacin and its metabolites (ciprofloxacin) in Jiangshan black-bone chickens, 76 Jiangshan black-bone chickens, of similar size, half roosters and half hens, were raised under the same feeding conditions until 97-107 days of age. Enrofloxacin soluble powder was administered continuously at a dose of 0.75 g per liter of drinking water for 5 d. Samples of muscle, liver, kidney and sebum were collected at 5, 7, 10, 14, 21, 28 and 42 d after drug withdrawal. The residues of enrofloxacin and its metabolites were detected using liquid chromatography-tandem mass spectrometry. The withdrawal periods for enrofloxacin and its metabolite in roosters and hens were calculated using WT1.4 software. The results showed that the withdrawal periods in hens were 148.2, 17.6, 57.4 and 232.3 d for muscle, liver, kidney and sebum, respectively; in roosters, the withdrawal periods were 115.2, 19.1, 60.7 and 259.9 d, respectively. To ensure that enrofloxacin residues in Jiangshan black-bone chicken meet the regulatory requirements, it is recommended that the withdrawal period be set at no less than 260 d.

Key words: enrofloxacin, residue, Jiangshan black-bone chicken, withdrawal period

CLC Number:

S831

QIAN Lingling, QIAN Mingrong, LIN Hui, LI Rui, ZHANG Hu, YU Ziqing, WANG Jianmei. Study on the residue elimination pattern of enrofloxacin and its metabolites in Jiangshan black-bone chickens[J]. Acta Agriculturae Zhejiangensis, 2026, 38(4): 781-787.

Figures/Tables 8

References 20

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t/min	流速/(mL·min^-1) Flow rate/(mL·min^-1)	A/%	B/%
0	0.40	5	95
2.0	0.40	20	80
6.5	0.40	99	1
8.0	0.40	99	1
8.1	0.40	5	95
10.0	0.40	5	95

t/min	流速/(mL·min^-1) Flow rate/(mL·min^-1)	A/%	B/%
0	0.40	5	95
2.0	0.40	20	80
6.5	0.40	99	1
8.0	0.40	99	1
8.1	0.40	5	95
10.0	0.40	5	95

药物 Drug	定性离子对(m/z) Qualitative ion pairs(m/z)	定量离子对(m/z) Quantitative ion pairs(m/z)	去簇电压/V Declustering potential/V	碰撞能量/eV Collision energy/eV
恩诺沙星	360.1>316.0	360.1>316.0	80	23
Enrofloxacin	360.1>245.4			40
环丙沙星	332.0>314.0	332.0>314.0	80	25
Ciprofloxacin	332.0>231.0			45

药物 Drug	定性离子对(m/z) Qualitative ion pairs(m/z)	定量离子对(m/z) Quantitative ion pairs(m/z)	去簇电压/V Declustering potential/V	碰撞能量/eV Collision energy/eV
恩诺沙星	360.1>316.0	360.1>316.0	80	23
Enrofloxacin	360.1>245.4			40
环丙沙星	332.0>314.0	332.0>314.0	80	25
Ciprofloxacin	332.0>231.0			45

组织 Tissue	恩诺沙星Enrofloxacin				环丙沙星Ciprofloxacin
组织 Tissue	标准曲线方程 Calibration curve equation	相关系数 Correlation coefficient	回收率/% Recovery/ %	相对偏差/% Relative deviation/%	标准曲线方程 Calibration curve equation	相关系数 Correlation coefficient	回收率/% Recovery/ %	相对偏差/% Relative deviation/%
肌肉Muscle	y=257 920x+207 389	0.999 8	78.9	9.2	y=481 424x+643 445	0.999 1	74.4	3.8
肝Liver	y=147 931x+42 976	0.999 9	68.6	11.3	y=269 635x+59 372	0.999 0	78.3	9.3
肾Kidney	y=182 878x+92 254	1.000 0	74.5	3.2	y=344 484x+55 745	0.999 9	72.2	4.3
皮脂Sebum	y=150 087x+84 190	0.999 9	73.0	4.8	y=263 916x+11 177	0.998 9	71.7	2.1

Study on the residue elimination pattern of enrofloxacin and its metabolites in Jiangshan black-bone chickens

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停药后时间/d Time after withdrawal/d	恩诺沙星残留总量/(μg·kg^-1)Total enrofloxacin residues/(μg·kg^-1)
停药后时间/d Time after withdrawal/d	肌肉Muscle	肝Liver	肾Kidney	皮脂Sebum
5	463.6±88.8 b	287.7±92.1 a	1 490.3±589.2 a	6 578.3±2 099.9 a
7	482.5±114.2 b	225.6±52.1 a	1 595.7±366.3 a	5 326.2±2 711.5 ab
10	516.2±160.4 b	74.9±16.0 b	1 453.4±364.9 a	4 479.3±2 499.1 abc
14	848.0±164.3 a	75.4±27.6 b	889.8±484.3 ab	3 558.2±1 475.1 abc
21	590.7±170.9 b	42.2±9.27 b	636.8±300.5 b	1 644.5±496.0 c
28	345.6±91.0 bc	36.2±6.24 b	382.9±170.1 b	1 710.7±815.5 c
42	196.1±71.3 c	20.4±6.05 b	245.8±149.5 b	2 347.5±1 396.9 bc

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组织 Tissue	恩诺沙星Enrofloxacin				环丙沙星Ciprofloxacin
组织 Tissue	标准曲线方程 Calibration curve equation	相关系数 Correlation coefficient	回收率/% Recovery/ %	相对偏差/% Relative deviation/%	标准曲线方程 Calibration curve equation	相关系数 Correlation coefficient	回收率/% Recovery/ %	相对偏差/% Relative deviation/%
肌肉Muscle	y=223 539x+92 834	0.996 2	84.4	0.8	y=493 511x+92 496	0.998 3	82.6	8.3
肝Liver	y=126 668x+99 024	0.999 7	74.8	8.7	y=222 367x+65 713	0.993 4	66.8	6.7
肾Kidney	y=177 291x+96 462	0.999 0	78.1	3.5	y=314 574x+70 553	0.997 4	66.9	3.8
皮脂Sebum	y=177 370x+91 073	0.999 6	75.7	13.9	y=285 097x+223 774	0.999 3	73.2	11.7

组织 Tissue	恩诺沙星Enrofloxacin				环丙沙星Ciprofloxacin
组织 Tissue	标准曲线方程 Calibration curve equation	相关系数 Correlation coefficient	回收率/% Recovery/ %	相对偏差/% Relative deviation/%	标准曲线方程 Calibration curve equation	相关系数 Correlation coefficient	回收率/% Recovery/ %	相对偏差/% Relative deviation/%
肌肉Muscle	y=223 539x+92 834	0.996 2	84.4	0.8	y=493 511x+92 496	0.998 3	82.6	8.3
肝Liver	y=126 668x+99 024	0.999 7	74.8	8.7	y=222 367x+65 713	0.993 4	66.8	6.7
肾Kidney	y=177 291x+96 462	0.999 0	78.1	3.5	y=314 574x+70 553	0.997 4	66.9	3.8
皮脂Sebum	y=177 370x+91 073	0.999 6	75.7	13.9	y=285 097x+223 774	0.999 3	73.2	11.7

停药后时间/d Time after withdrawal/d	恩诺沙星残留总量/(μg·kg^-1)Total enrofloxacin residues/(μg·kg^-1)
停药后时间/d Time after withdrawal/d	肌肉Muscle	肝Liver	肾Kidney	皮脂Sebum
5	371.1±166.1 ab	230.0±85.0 a	1 509.1±729.0 a	5 525.0±786.3 ab
7	337.4±201.0 ab	212.8±116.0 ab	1 649.3±962.4 a	5 341.5±1 582.0 abc
10	383.8±169.3 ab	113.4±40.5 bc	1 578.0±551.4 a	6 404.1±905.6 a
14	508.9±175.6 a	62.8±32.3 c	623.6±317.8 b	4 692.3±1 813.5 abc
21	317.6±118.3 b	69.0±48.2 c	339.7±218.6 b	3 185.3±1 335.1 cd
28	183.0±78.2 b	30.3±13.6 c	213.3±140.0 b	3 350.2±609.5 bcd
42	139.2±50.0 b	11.4±3.2 c	223.8±123.1 b	2 452.7±359.6 d

停药后时间/d Time after withdrawal/d	恩诺沙星残留总量/(μg·kg^-1)Total enrofloxacin residues/(μg·kg^-1)
停药后时间/d Time after withdrawal/d	肌肉Muscle	肝Liver	肾Kidney	皮脂Sebum
5	371.1±166.1 ab	230.0±85.0 a	1 509.1±729.0 a	5 525.0±786.3 ab
7	337.4±201.0 ab	212.8±116.0 ab	1 649.3±962.4 a	5 341.5±1 582.0 abc
10	383.8±169.3 ab	113.4±40.5 bc	1 578.0±551.4 a	6 404.1±905.6 a
14	508.9±175.6 a	62.8±32.3 c	623.6±317.8 b	4 692.3±1 813.5 abc
21	317.6±118.3 b	69.0±48.2 c	339.7±218.6 b	3 185.3±1 335.1 cd
28	183.0±78.2 b	30.3±13.6 c	213.3±140.0 b	3 350.2±609.5 bcd
42	139.2±50.0 b	11.4±3.2 c	223.8±123.1 b	2 452.7±359.6 d