Sensitivity effect analysis of different highly pathogenic Porcine reproductive and respiratory syndrome virus samples on cells

›› 2011, Vol. 23 ›› Issue (1): 62-65.

• 动物科学 • Previous Articles Next Articles

Sensitivity effect analysis of different highly pathogenic Porcine reproductive and respiratory syndrome virus samples on cells

ZHANG Chun-ling;ZOU Yong;ZHANG Wan-hua;ZHOU Zong-qing;LI Chun-hua;JIANG Feng-ying

Animal Husbandry and Veterinary Research Institute, shanghai Academy of Agricultural Sciences, Shanghai 201106, China

Received:1900-01-01 Revised:1900-01-01 Online:2011-01-25 Published:2011-01-25

Abstract

Abstract: We collected 100 clinical samples from suspected Porcine reproductive and respiratory syndrome virus(PRRSV) infection occurred in pig farms for virus isolation. The positive samples of PRRSV detected by RT-PCR were divided into two groups by serum and tissue samples. PRRSV were isolated and cultured with Marc-145 and PAM cell. The results showed that 29 PRRSV, including 21 serum samples (72.5%) and 8 tissue samples (27.5%), were obtained from the 45 positive samples with cell culture. Nineteen and 24 PRRSV strains were respectively isolated with Marc-145 cell and PAM cell, in which 10 PRRSV strains were isolated with both Marc-145 and PAM cell. Data showed that serum was the ideal sample of isolating PRRSV, while the virus had no significant difference in sensitivity effect on Marc-145 and PAM cells.

Key words: RRSV, cell, sensitivity analysis

ZHANG Chun-ling;ZOU Yong;ZHANG Wan-hua;ZHOU Zong-qing;LI Chun-hua;JIANG Feng-ying. Sensitivity effect analysis of different highly pathogenic Porcine reproductive and respiratory syndrome virus samples on cells[J]. , 2011, 23(1): 62-65.

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Sensitivity effect analysis of different highly pathogenic Porcine reproductive and respiratory syndrome virus samples on cells

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