不同浓度EGCG对NaCl胁迫下黄瓜种子萌发及其抗性的影响

doi:10.3969/j.issn.1004-1524.2018.07.08

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (7): 1160-1167.DOI: 10.3969/j.issn.1004-1524.2018.07.08

不同浓度EGCG对NaCl胁迫下黄瓜种子萌发及其抗性的影响

李洋^{1, 2}, 刘凯¹, 魏吉鹏², 张兰², 李鑫^{2, *}, 韩文炎^{2, *}, 李青云^{1, *}

1.河北农业大学园艺学院,河北保定 071000;
2.中国农业科学院茶叶研究所,浙江杭州310008

收稿日期:2017-12-10 出版日期:2018-07-20 发布日期:2018-08-02
通讯作者: 李鑫,E-mail: lixin@tricaas.com;韩文炎,E-mail: hanwy@tricaas.com;李青云,E-mail: liqingyun69@163.com
作者简介:李洋(1990—),女,山东青岛人,硕士研究生,主要从事蔬菜栽培生理生化研究。E-mail: liyang902102@163.com

Effects of various concentrations of EGCG on seed germination and resistance in cucumber under NaCl stress

LI Yang^{1, 2}, LIU Kai¹, WEI Jipeng², ZHANG Lan², LI Xin^{2, *}, HAN Wenyan^{2, *}, LI Qingyun^{1, *}

1.Horticulture College, Hebei Agricultural University, Baoding 071000, China;
2. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China

Received:2017-12-10 Online:2018-07-20 Published:2018-08-02

摘要/Abstract

摘要： 以黄瓜为实验材料,研究不同浓度表没食子儿茶素没食子酸酯(EGCG)对NaCl胁迫下黄瓜种子萌发、丙二醛(MDA)含量及胚根抗氧化酶活性的影响。结果表明,在非盐胁迫条件下,与对照相比,不同浓度 EGCG(10、100、1 000 μmol·L^-1)处理对黄瓜种子萌发、主根伸长及侧根的发生具有一定的浓度效应。在200 mmol·L^-1 NaCl处理条件下,对照组黄瓜种子萌发、主根伸长及侧根发生均显著受抑制,同时MDA含量和抗氧化酶活性显著上升;而不同浓度EGCG处理能明显缓解NaCl对黄瓜种子萌发、主根伸长及侧根发生造成的抑制作用,同时降低NaCl胁迫引起的MDA含量,以100 μmol·L^-1 EGCG处理效果最佳。测定黄瓜胚根抗氧化酶活性发现,不同浓度EGCG可能通过提高APX、CAT及SOD活性来降低NaCl胁迫下黄瓜的MDA含量。因此,外源添加EGCG可以促进黄瓜种子萌发,可能是通过提高胚根抗氧化酶活性来缓解NaCl引起的根系盐胁迫。

关键词: 表没食子儿茶素没食子酸酯, 黄瓜, 盐胁迫, 种子萌发, 丙二醛, 抗氧化酶活性

Abstract: The present study investigated the effects of exogenous application with various concentrations of EGCG (epigallocatechin gallate) on seed germination, malondialdehyde (MDA) content and activities of antioxidant enzymes in radicles of cucumber under NaCl stress. Results showed that, without salt stress, seed germination, radicle length and root branch number responded differently to various concentrations of EGCG. While those were significantly restrained under 200 mmol·L^-1 NaCl treatment, accompanying the great increases in content of MDA and activities of antioxidant enzymes. Furthermore, application with various concentrations of EGCG(10, 100, 1 000 μmol·L^-1)could significantly increase seed germination, radicle length and root branch number under NaCl stress. Moreover, EGCG application decreased MDA content by improving the activities of antioxidant enzymes (APX, CAT, SOD and POD) under NaCl stress. And the treatment with 100 μmol·L^-1 EGCG performed best. These results suggested that exogenous EGCG could enhance seed germination and mitigate NaCl-induced salt stress through promoting the activities of antioxidant enzymes in cucumber.

Key words: EGCG, cucumber, NaCl, salt stress, seed germination, MDA, antioxidant enzyme activity

中图分类号:

S642.2

李洋, 刘凯, 魏吉鹏, 张兰, 李鑫, 韩文炎, 李青云. 不同浓度EGCG对NaCl胁迫下黄瓜种子萌发及其抗性的影响[J]. 浙江农业学报, 2018, 30(7): 1160-1167.

LI Yang, LIU Kai, WEI Jipeng, ZHANG Lan, LI Xin, HAN Wenyan, LI Qingyun. Effects of various concentrations of EGCG on seed germination and resistance in cucumber under NaCl stress[J]. , 2018, 30(7): 1160-1167.

参考文献

[1] 刘维宝, 陈新红, 周羽, 等. 外源水杨酸对NaCl胁迫下黄瓜种子萌发和幼苗根系生长的影响[J]. 中国农学通报, 2013,29(34): 166-170.
LIU W B, CHEN X H, ZHOU Y, et al. Effect of salicylic acid on cucumber seed germination and seedling root growth under NaCl stress[J]. Chinese Agricultural Science Bulletin , 2013, 29(34): 166-170. (in Chinese with English abstract)
[2] 吕杰, 王秀峰, 魏珉, 等. 不同盐处理对黄瓜幼苗生长及生理特性的影响[J]. 植物营养与肥料学报, 2007, 13(6): 1123-1128.
LYU J, WANG X F, WEI M, et al. Effect of different salt treatments on growth and physiological characteristics of cucumber seedlings[J]. Plant Nutrition and Fertilizer Science , 2007, 13(6): 1123-1128. (in Chinese with English abstract)
[3] ZHU J K. Abiotic stress signaling and responses in plants[J]. Cell , 2016, 167(2):313-324.
[4] TAYLOR L, GROTEWOLD E. Flavonoids as developmental regulators[J]. Current Opinions in Plant Biology , 2005, 8(3): 317-323.
[5] 贾振华. 植物黄酮类化合物槲皮素与转录因子AtMYB44诱导和调控植物防卫反应的研究[D]. 南京: 南京农业大学, 2010.
JIA Z H. Studies on roles of flavonoid quercetin and transcription factor AtMYB44 in induction and regulation of defense responses in Arabidopsis thaliana [D]. Nanjing: Nanjing Agricultural University, 2010. (in Chinese with English abstract)
[6] 赵丽萍, 邵宛芳. 茶叶中EGCG功效研究进展[J]. 中国农学通报, 2007, 23(7): 143-147.
ZHAO L P, SHAO W F. Research effect of epigallocatech in gallate in the tea[J]. Chinese Agricultural Science Bulletin , 2007, 23(7): 143-147. (in Chinese with English abstract)
[7] YANG C S, WANG X, LU G, et al. Cancer prevention by tea: animal studies, molecular mechanisms and human relevance[J]. Nature Reviews Cancer , 2009, 9(6): 429-439.
[8] RANI A, KUMAR S, SHARMA M, et al. Catechin promotes growth of Arabidopsis thaliana with concomitant changes in vascular system, photosynthesis and hormone content[J]. Biologia Plantarum , 2011, 55(4): 779-782.
[9] HONG G J, WANG J, HOCHSTETTER D, et al. Epigallocatechin-3-gallate functions as a physiological regulator by modulating the jasmonic acid pathway[J]. Physiologia Plantarum , 2015, 153(3): 432-439.
[10] 赵艳艳,胡晓飞,邹志荣,等.不同浓度5-氨基乙酰丙酸(ALA)浸种对NaCl胁迫下番茄种子发芽率及芽苗生长的影响[J]. 生态学报,2013, 33(1): 62-70.
ZHAO Y Y,HU X F,ZOU Z R,et al. Effects of seed soaking with different concentrations of 5-aminolevulinic acid on the germination of tomato( Solanum lycopersicum )seeds under NaCl stress[J]. Acta Ecologica Sinica , 2013, 33(1): 62-70. (in Chinese with English abstract)
[11] WANG S D, ZHU F, YUAN S, et al. The roles of ascorbic acid and glutathione in symptom alleviation to SA-deficient plants infected with RNA viruses[J]. Planta , 2011, 234(1):171-181.
[12] 张治安. 植物生理实验指导[M]. 北京: 中国农业科学出版社, 2004.
[13] NAKANO Y, ASADA K. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts[J]. Plant & Cell Physiology , 1981, 22(5): 867-880.
[14] PATRA HK, KAR M, MISHRA D. Catalase activity in leaves and cotyle dons during plant development and senescence[J]. Biochemie und Physiologie der Pflanzen ,1978, 172(4): 384-390.
[15] STEWARD RRC, BEWLEY JD. Lipid peroxidation associated with accelerated aging of soybean axes[J]. Plant Physiology ,1980, 65(2): 245-248.
[16] CAKMAK I, MARSCHNER H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bea leaves[J]. Plant Physiology , 1992,98(4):1222-1227.
[17] BRADFORD M M. A rapid and sensitive method for the quantities of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry , 1976, 72: 248-254.
[18] 侯梁宇, 王兴鹏. NaCl胁迫对黄瓜种子萌发的影响[J]. 安徽农业科学, 2014, 42(24): 8123-8124.
HOU L Y, WANG X P. Effects of NaCl stress on seed germination of cucumber[J]. Journal of Anhui Agricultural Sciences , 2014, 42(24): 8123-8124.( in Chinese with English abstract)
[19] KANMAZ E Ö,OVA G. The influence of germination time on SDG lignan, phenolic and flavonoid contents of flaxseed ( Linum usitatissimum L.)sprouts[J]. GIDA-Journal of Food , 2015, 40(5): 255-262.
[20] 侯建霞. 苦荞麦中活性成分及其在萌发过程中变化的研究[D]. 无锡: 江南大学, 2007.
HOU J X. Study on active components of buckwheat and theirs changes during seeding progress[D]. Wuxi: Jiangnan University, 2007. (in Chinese with English abstract)
[21] 郭亚姣, 郑书馨, 李洁, 等. 黄顶菊叶中类黄酮对白菜种子萌发的影响[J]. 内蒙古农业大学学报(自然科学版), 2011, 32(4): 105-109.
GUO Y J, ZHENG S X, LI J, et al. The effects of flavonoids extracted from Flaveria bidentis leaves on seed germination of Brassica pekinensi [J]. Journal of Inner Mongolia Agricultural University ( Natural Science Edition ), 2011, 32(4): 105-109. (in Chinese with English abstract)
[22] SHU K, QI Y, CHEN F, et al. Salt stress represses soybean seed germination by negatively regulating GA biosynthesis while positively mediating ABA biosynthesis[J]. Frontiers in Plant Science , 2017,8: 1372.
[23] MA Q, KANG J, LONG R, et al. Comparative proteomic analysis of alfalfa revealed new salt and drought stress-related factors involved in seed germination[J]. Molecular Biology Reports , 2017, 44(3): 261-272.
[24] RANI A, KUMAR V S, SHARMA M, et al. Catechin promotes growth of Arabidopsis thaliana with concomitant changes in vascular system, photosynthesis and hormone content[J]. Biologia Plantarum , 2011, 55 (4): 779-782.
[25] 宋慧, 冯佰利, 高小丽, 等. 不同小豆品种(系)叶片衰老与活性氧代谢[J]. 作物学报, 2010, 36(2): 347-353.
SONG H, FENG B L, GAO X L, et al. Leaf senescence and reactive oxygen metabolism in different adzuki bean cultivars (lines)[J]. Acta Agronomica Sinica , 2010, 36(2): 347-353. (in Chinese with English abstract)
[26] 邹琦. 植物生理学试验指导[M]. 北京:中国农业出版社, 2000.
[27] 李晓清, 胡学煜, 左英强, 等. 热胁迫对植物生理影响的研究进展[J]. 西南林学院学报, 2009, 29(6):72-76.
LI X Q, HU X Y, ZUO Y Q, et al. Research advances in physiological effect of heat stress on plant[J]. Journal of Southwest Forestry University , 2009, 29(6):72-76. (in Chinese with English abstract)
[28] 张治平, 於丙军, 汪良驹, 等. 低温下ALA对番茄光合色素和抗氧化酶活性的影响[J]. 江苏农业学报, 2014, 30(1): 222-224.
ZHANG Z P, YU B J,WANG L J, et al. Effect of 5-aminolevulinic acid on photosynthetic pigments and activities of antioxidant enzymes of tomato under chilling stress[J]. Jiangsu Journal of Agricultural Sciences , 2014, 30(1): 222-224. (in Chinese with English abstract)
[29] KRYSTYNA M J,MILENA C,ANNA P, et al. Constitutive elevated accumulation of phenylpropanoids in soybean roots at low temperature[J]. Plant Science , 2002, 163(2):369-373.
[30] HEMANDEZ I,ALEGRE L,MUNNE B S. Drought-induced changes in flavonoids and other low molecular weights antioxidants in Cistus clusii grown under Mediterranean field conditions[J]. Tree Physiology , 2004, 24(11): 1303-1311.
[31] 毛美琴, 赵燕, 魏玉兰, 等. 滇重楼根水浸液对四种园林植物的化感作用研究[J]. 浙江农业学报, 2018, 30(1): 80-90.
MAO M Q, ZHAO Y, WEI Y L, et al. Allelopathy of aqueous extracts from Paris polyphylla var. Yunnanensis roots on four ornamental plants[J]. Acta Agriculturae Zhejiangensis , 2018, 30(1): 80-90.(in Chinese with English abstract)
[32] YIU J C, TSENG M J, LIU C W. Exogenous catechin increases antioxidant enzyme activity and promotes flooding tolerance in tomato ( Solanum lycopersicum L.)[J]. Plant and Soil , 2011, 344(1/2): 213-225.
[33] BAIS H P, WALKER T S, KENNAN A J, et al. Structure-dependent phytotoxicity of catechins and other flavonoids: flavonoid conversions by cell-free protein extracts of Centaurea maculosa ( spotted knapweed )roots[J]. Journal of Agricultural and Food Chemistry , 2003, 51(4): 897-901.

不同浓度EGCG对NaCl胁迫下黄瓜种子萌发及其抗性的影响

Effects of various concentrations of EGCG on seed germination and resistance in cucumber under NaCl stress

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	陆安桥, 张峰举, 王学琴, 许兴. 盐胁迫对苗期湖南稷子K ⁺、Na ⁺含量与分布的影响[J]. 浙江农业学报, 2021, 33(3): 396-403.
[2]	刘如, 董畅茹, 张祎雯, 屈铭慧, 张伟, 洒海洋, 陈海燕, 叶文玲, 樊霆. 镉胁迫下黑曲霉TL-F2的促生特征及其对黑麦草种子萌发、幼苗生长和镉含量的影响[J]. 浙江农业学报, 2021, 33(2): 326-334.
[3]	宋新丹, 陈斌斌, 马增岭, 徐丽丽, 林立东, 吴明江. 盐度对羊栖菜(Sargassum fusiforme)幼体光合特性的影响[J]. 浙江农业学报, 2020, 32(9): 1634-1644.
[4]	牛博, 李丽娜, 庞广昌, 鲁丁强. 植物根尖分生组织传感器的构建及其对尿素传感动力学研究[J]. 浙江农业学报, 2020, 32(8): 1466-1474.
[5]	石婧, 刘东洋, 张凤华. 棉花幼苗对盐胁迫的生理响应与耐盐机理[J]. 浙江农业学报, 2020, 32(7): 1141-1148.
[6]	兰挚谦, 张凯歌, 张雪艳. 耕层厚度对黄瓜叶片光合荧光与根系生理特性的影响[J]. 浙江农业学报, 2020, 32(7): 1196-1205.
[7]	朱森林, 王丹媚, 唐秀梅, 陈瑞, 杨蓉, 徐月妹, 金璐懿, 任晴雯, 刘鹏, 罗军. 木霉水分散粒剂的培养条件优化及其对黄瓜枯萎病的防治效果[J]. 浙江农业学报, 2020, 32(6): 1009-1018.
[8]	林辉, 张锦, 原倩宇, 叶静, 孙万春, 虞轶俊, 俞巧钢, 马军伟. 棘孢木霉和超微粉腐殖质改善连作土壤微生态[J]. 浙江农业学报, 2020, 32(6): 1060-1069.
[9]	刘艺平, 苏少文, 张琳, 刘莹, 黄志远, 贺丹, 孔德政. 外源钙对荷花适应盐胁迫的影响[J]. 浙江农业学报, 2020, 32(2): 243-252.
[10]	金宁, 吕剑, 郁继华, 金莉, 张国斌, 肖雪梅, 胡琳莉. 基质栽培黄瓜叶片水分状况、光合与荧光参数对不同灌水下限的响应[J]. 浙江农业学报, 2020, 32(12): 2162-2172.
[11]	金宁, 吕剑, 郁继华, 金莉, 张国斌, 肖雪梅, 胡琳莉. 基质栽培黄瓜叶片水分状况、光合与荧光参数对不同灌水下限的响应[J]. 浙江农业学报, 2020, 32(12): 2162-2172.
[12]	边建文, 崔岩, 杨宋琪, 罗光宏, 孟宪刚. 衣藻和固氮鱼腥藻对盐胁迫下小麦幼苗生长的影响[J]. 浙江农业学报, 2020, 32(10): 1748-1756.
[13]	朱晓林, 魏小红, 王宝强, 王贤, 张明君. c-GMP诱导对盐胁迫下番茄的转录组分析[J]. 浙江农业学报, 2020, 32(10): 1788-1797.
[14]	方芳, 何序晨, 张志豪, 张勤, 关亚静, 胡晋, 胡伟民. 玉米自交系苗期对高温胁迫的响应机制及其抗逆性[J]. 浙江农业学报, 2019, 31(7): 1045-1056.
[15]	王哲, 柴里昂, 樊怀福, 杜长霞. 植物响应盐胁迫蛋白质组学研究进展[J]. 浙江农业学报, 2019, 31(6): 1021-1028.