镉胁迫下黑曲霉TL-F2的促生特征及其对黑麦草种子萌发、幼苗生长和镉含量的影响

doi:10.3969/j.issn.1004-1524.2021.02.16

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (2): 326-334.DOI: 10.3969/j.issn.1004-1524.2021.02.16

镉胁迫下黑曲霉TL-F2的促生特征及其对黑麦草种子萌发、幼苗生长和镉含量的影响

刘如(), 董畅茹, 张祎雯, 屈铭慧, 张伟, 洒海洋, 陈海燕, 叶文玲, 樊霆^*()

安徽农业大学资源与环境学院,农田生态保育与污染防控安徽省重点实验室,安徽合肥 230036

收稿日期:2020-08-17 出版日期:2021-02-25 发布日期:2021-02-25
通讯作者: 樊霆
作者简介:*樊霆,E-mail: fanting@ahau.edu.cn
刘如(1994—),女,安徽五河人,硕士,主要从事环境污染微生物修复研究。E-mail: 2992898375@qq.com
基金资助:
国家重点研发计划(2019YFC1805203);国家自然科学基金(41101485);安徽省高等学校自然科学研究项目(K1952001);安徽省农田生态保育与污染防控安徽省重点实验室开放基金(FECPP201903);国家级大学生创新创业训练计划(202010364065);安徽省大学生创新创业训练计划(201910364228);安徽农业大学大学生创新基金(XJDC2019207);安徽农业大学大学生创新基金(XJDC2020463)

Growth-promoting characteristics of Aspergillus niger TL-F2 and its effect on seed germination and cadmium content in seedlings of ryegrass under cadmium stress

LIU Ru(), DONG Changru, ZHANG Yiwen, QU Minghui, ZHANG Wei, SA Haiyang, CHEN Haiyan, YE Wenling, FAN Ting^*()

Anhui Province Key Lab of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China

Received:2020-08-17 Online:2021-02-25 Published:2021-02-25
Contact: FAN Ting

摘要/Abstract

摘要：

以黑曲霉(Aspergillus niger)TL-F2为供试菌株,黑麦草为供试植物,研究Cd胁迫下A. niger TL-F2的促生特性,以及对黑麦草种子萌发、幼苗生长和Cd含量的影响。结果表明:与不加Cd的对照组相比,低浓度Cd(5 mg·L^-1)胁迫下,A. niger TL-F2产吲哚乙酸(IAA)和溶磷的能力无显著变化,但产铁载体的能力显著(P<0.05)降低48.18%;中(20 mg·L^-1)、高(50 mg·L^-1)浓度Cd胁迫下,A. niger TL-F2产IAA的能力分别显著(P<0.05)降低55.76%和65.69%,溶磷量分别显著(P<0.05)降低50.07%和78.19%,产铁载体的能力分别显著(P<0.05)降低69.71%和80.08%。接种高浓度(1×10⁸ mL^-1)A. niger TL-F2有助于促进Cd胁迫下黑麦草种子的萌发和生长,高浓度Cd胁迫下,黑麦草地上部Cd含量较不接菌的显著(P<0.05)增加,增幅为17.95%;接种低浓度(1×10⁶ mL^-1)A. niger TL-F2对黑麦草种子萌发、Cd含量无明显促进作用。综合来看,接种适当量的A. niger TL-F2有助于促进Cd胁迫下黑麦草种子的萌发和生长,增加黑麦草地上部Cd含量,可作为黑麦草修复Cd污染水体和土壤的强化措施。

关键词: 黑麦草, 镉, 黑曲霉, 种子萌发, 促生特性

Abstract:

The growth-promoting characteristics of Aspergillus niger TL-F2, and its effects on seed germination, seedling growth and Cd content of ryegrass under Cd stress were studied. The results showed that the siderophore production of A. niger TL-F2 was significantly (P<0.05) decreased by 48.18%, and there was no significant change of indoleacetic acid (IAA) production and phosphate solubilization under low Cd (5 mg·L^-1) stress. However, under medium (20 mg·L^-1) and high (50 mg·L^-1) Cd stress, the IAA production, phosphate solubilization and siderophore production of A. niger TL-F2 was significantly (P<0.05) decreased by 55.76% and 65.69%, 50.07% and 78.19%, 69.71% and 80.08%, respectively. Inoculation of 1×10⁸ mL^-1 A. niger TL-F2 could benefit the seed germination and seedling growth of ryegrass, and the Cd content in shoots was significantly (P<0.05) increased by 17.95% than that without inoculation under high Cd stress. However, inoculation of 1×10⁶ mL^-1 A. niger TL-F2 showed no significant effect on seed germination and seedling growth of ryegrass. The results suggested that inoculation of proper amount of A. niger TL-F2 could promote seed germination and seedling growth of ryegrass, and enhance the accumulation of Cd in the shoots of ryegrass seedlings. Therefore, inoculation of A. niger TL-F2 had the potential as a new means to enhance the remediation of Cd polluted soil and water by using ryegrass.

Key words: ryegrass, cadmium, Aspergillus niger, seed germination, growth-promoting characteristics

中图分类号:

X172

刘如, 董畅茹, 张祎雯, 屈铭慧, 张伟, 洒海洋, 陈海燕, 叶文玲, 樊霆. 镉胁迫下黑曲霉TL-F2的促生特征及其对黑麦草种子萌发、幼苗生长和镉含量的影响[J]. 浙江农业学报, 2021, 33(2): 326-334.

LIU Ru, DONG Changru, ZHANG Yiwen, QU Minghui, ZHANG Wei, SA Haiyang, CHEN Haiyan, YE Wenling, FAN Ting. Growth-promoting characteristics of Aspergillus niger TL-F2 and its effect on seed germination and cadmium content in seedlings of ryegrass under cadmium stress[J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 326-334.

图/表 6

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Cd/ (mg·L^-1)	菌浓度 Stain content/mL^-1	GV/%	GR/%	GI	VI	TI	SI
0	0	72±2 ab	90±1 a	45.03±0.93 a	736.22±15.18 b	1.00±0.01 ab	0 de
0	1×10⁶	66±4 bcd	88±3 a	42.80±2.04 ab	692.11±32.93 c	1.00±0.15 ab	-0.01±0.15 de
0	1×10⁸	78±3 a	90±3 a	46.22±1.43 a	839.05±26.01 a	1.13±0.16 a	-0.13±0.16 e
5	0	70±2 abc	90±2 a	44.39±0.95 ab	592.68±12.62 d	0.82±0.06 bc	0.18±0.03 cd
5	1×10⁶	66±4 bcd	88±2 a	42.69±1.08 ab	604.43±15.34 d	0.87±0.06 b	0.13±0.06 d
5	1×10⁸	74±3 ab	90±3 a	45.11±1.82 a	828.25±33.27 a	1.14±0.18 a	-0.14±0.18 e
20	0	60±3 cd	84±6 a	40.45±3.15 ab	251.19±19.57 f	0.38±0.04 d	0.62±0.04 b
20	1×10⁶	56±9 d	84±5 a	39.13±2.88 b	191.362±14.06 g	0.30±0.02 d	0.70±0.02 b
20	1×10⁸	66±5 bcd	88±2 a	42.77±1.69 ab	425.98±16.81 e	0.62±0.20 c	0.38±0.20 b
50	0	32±6 f	64±7 b	26.37±3.47 c	0 h	0 e	1.00 a
50	1×10⁶	28±6 f	64±6 b	26.45±3.02 c	0 h	0 e	1.00 a
50	1×10⁸	44±7 e	64±8 b	30.19±4.12 c	0 h	0 e	1.00 a

Cd/ (mg·L^-1)	菌浓度 Stain content/mL^-1	GV/%	GR/%	GI	VI	TI	SI
0	0	72±2 ab	90±1 a	45.03±0.93 a	736.22±15.18 b	1.00±0.01 ab	0 de
0	1×10⁶	66±4 bcd	88±3 a	42.80±2.04 ab	692.11±32.93 c	1.00±0.15 ab	-0.01±0.15 de
0	1×10⁸	78±3 a	90±3 a	46.22±1.43 a	839.05±26.01 a	1.13±0.16 a	-0.13±0.16 e
5	0	70±2 abc	90±2 a	44.39±0.95 ab	592.68±12.62 d	0.82±0.06 bc	0.18±0.03 cd
5	1×10⁶	66±4 bcd	88±2 a	42.69±1.08 ab	604.43±15.34 d	0.87±0.06 b	0.13±0.06 d
5	1×10⁸	74±3 ab	90±3 a	45.11±1.82 a	828.25±33.27 a	1.14±0.18 a	-0.14±0.18 e
20	0	60±3 cd	84±6 a	40.45±3.15 ab	251.19±19.57 f	0.38±0.04 d	0.62±0.04 b
20	1×10⁶	56±9 d	84±5 a	39.13±2.88 b	191.362±14.06 g	0.30±0.02 d	0.70±0.02 b
20	1×10⁸	66±5 bcd	88±2 a	42.77±1.69 ab	425.98±16.81 e	0.62±0.20 c	0.38±0.20 b
50	0	32±6 f	64±7 b	26.37±3.47 c	0 h	0 e	1.00 a
50	1×10⁶	28±6 f	64±6 b	26.45±3.02 c	0 h	0 e	1.00 a
50	1×10⁸	44±7 e	64±8 b	30.19±4.12 c	0 h	0 e	1.00 a

Cd/ (mg·L^-1)	菌浓度 Stain content/mL^-1	根长 Root length/cm	芽长 Shoot length/cm	根干重 Root dry weight/g	芽干重 Shoot dry weight/g
0	0	5.45±0.75 ab	13.90±0.34 a	0.011±0.003 a	0.044±0.011 a
0	1×10⁶	5.39±0.69 ab	13.82±0.47 a	0.013±0.001 a	0.044±0.007 a
0	1×10⁸	6.05±0.42 a	14.50±0.72 a	0.014±0.001 a	0.044±0.007 a
5	0	4.45±0.47 b	12.16±0.93 b	0.013±0.001 a	0.042±0.002 a
5	1×10⁶	4.72±0.29 b	10.97±0.80 c	0.013±0.001 a	0.045±0.003 a
5	1×10⁸	6.12±0.77 a	12.36±0.81 b	0.015±0.003 a	0.042±0.008 a
20	0	2.07±0.51 d	9.37±0.85 d	0.011±0.002 a	0.037±0.004 a
20	1×10⁶	1.63±0.26 d	8.81±0.21 e	0.015±0.005 a	0.038±0.007 a
20	1×10⁸	3.32±1.08 c	9.95±0.92 cd	0.014±0.001 a	0.044±0.007 a
50	0	—	7.27±0.67 e	—	0.036±0.007 a
50	1×10⁶	—	5.83±0.07 f	—	0.034±0.005 a
50	1×10⁸	—	7.52±0.72 e	—	0.036±0.007 a

Cd/ (mg·L^-1)	菌浓度 Stain content/mL^-1	根长 Root length/cm	芽长 Shoot length/cm	根干重 Root dry weight/g	芽干重 Shoot dry weight/g
0	0	5.45±0.75 ab	13.90±0.34 a	0.011±0.003 a	0.044±0.011 a
0	1×10⁶	5.39±0.69 ab	13.82±0.47 a	0.013±0.001 a	0.044±0.007 a
0	1×10⁸	6.05±0.42 a	14.50±0.72 a	0.014±0.001 a	0.044±0.007 a
5	0	4.45±0.47 b	12.16±0.93 b	0.013±0.001 a	0.042±0.002 a
5	1×10⁶	4.72±0.29 b	10.97±0.80 c	0.013±0.001 a	0.045±0.003 a
5	1×10⁸	6.12±0.77 a	12.36±0.81 b	0.015±0.003 a	0.042±0.008 a
20	0	2.07±0.51 d	9.37±0.85 d	0.011±0.002 a	0.037±0.004 a
20	1×10⁶	1.63±0.26 d	8.81±0.21 e	0.015±0.005 a	0.038±0.007 a
20	1×10⁸	3.32±1.08 c	9.95±0.92 cd	0.014±0.001 a	0.044±0.007 a
50	0	—	7.27±0.67 e	—	0.036±0.007 a
50	1×10⁶	—	5.83±0.07 f	—	0.034±0.005 a
50	1×10⁸	—	7.52±0.72 e	—	0.036±0.007 a

镉胁迫下黑曲霉TL-F2的促生特征及其对黑麦草种子萌发、幼苗生长和镉含量的影响

Growth-promoting characteristics of Aspergillus niger TL-F2 and its effect on seed germination and cadmium content in seedlings of ryegrass under cadmium stress

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