玉米第8染色体单片段代换系的构建与灰斑病抗性材料筛选

doi:10.3969/j.issn.1004-1524.2021.03.01

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (3): 383-389.DOI: 10.3969/j.issn.1004-1524.2021.03.01

玉米第8染色体单片段代换系的构建与灰斑病抗性材料筛选

杨梅, 胡小兰, 申涛, 谭康, 刘代铃, 邱红波^*()

贵州大学农学院,贵州贵阳 550025

收稿日期:2020-11-13 出版日期:2021-03-25 发布日期:2021-03-25
作者简介:*, 邱红波,E-mail: qhb001122@163.com
杨梅(1995—),女,贵州平塘人,硕士研究生,研究方向为分子遗传育种。E-mail: 2452627869@qq.com
通讯作者: 邱红波
基金资助:
国家自然科学基金(32060488);国家自然科学基金(31460384);贵州大学培育项目(黔科合平台人才〔2007〕5788-26);贵州大学博士基金启动项目(贵大人基合字〔2013〕27号)

Construction of single fragment substitution lines of maize 8th chromosome and sreening of resistant maize germplasm to gray leaf spot

YANG Mei, HU Xiaolan, SHEN Tao, TAN Kang, LIU Dailing, QIU Hongbo^*()

College of Agriculture, Guizhou University, Guiyang 550025, China

Received:2020-11-13 Online:2021-03-25 Published:2021-03-25
Contact: QIU Hongbo

摘要/Abstract

摘要：

以贵州省常用玉米自交系T32为供体,J51为受体,通过连续多代回交并结合分子标记辅助选择,获得了23个玉米第8染色体单片段代换系。采用自然接种法对该群体进行灰斑病抗性鉴定,并选用在两亲本间均具明显多态性差异的65对SSR标记进行代换系的跟踪检测,通过第8染色体上的29个SSR标记对代换系供体片段进行遗传结构分析。结果表明,23个染色体单片段代换系中供体代换的位点不同,代换片段长度为10.40~129.70 cM,平均长度为36.99 cM,导入片段总长850.67 cM,对第8染色体的覆盖率为80.80%。自然接种灰斑病菌后产生了抗、中抗、感、高感4种表型,其中H3系和H17系发病程度较低,2年的病级均值分别为2.85、2.90,表现为抗病。研究筛选出的2个抗性株系可作为后续研究材料,为玉米灰斑病抗性基因挖掘与抗病育种创建基础。

关键词: 玉米, 第8染色体, 染色体片段代换系, 灰斑病, 抗性鉴定

Abstract:

In this study, 23 single segment substitution lines of maize 8th chromosome were obtained by successive backcross and marker assisted selection with T32 and J51, the common inbred lines of maize in Guizhou, as donor and acceptor, respectively. Then, method of natural inoculation was used to identify the resistance to maize gray spot, and 65 pairs of SSR markers that located on the 8th chromosome with obvious polymorphic differences between two parents were used for the following detection of substitution lines. The genetic structure of donor fragments of the substitution lines was evaluated by 29 SSR markers on the 8th chromosome. The results showed that 23 chromosome single-segment substitution lines had different donor fragment sites and length of the substitution fragment was 10.40-129.70 cM, the average length was 36.99 cM, total length of the imported fragment was 850.67 cM, and coverage rate of the replacement fragments on 8th chromosomes was 80.80%. Disease-resistant result showed four kinds of phenotypes: resistant, moderately resistant, susceptible and highly susceptible. Among them, the line of H3 and H17 were identified as resistant, with the two years average disease grade of 2.85 and 2.90, respectively. Two resistant lines were screened out in this study, which could be used as follow-up research materials and lay a foundation for resistant maize breeding to gray leaf spot.

Key words: maize, chromosome 8, chromosome segment substitution line, gray spot disease, resistance identification

中图分类号:

S513

杨梅, 胡小兰, 申涛, 谭康, 刘代铃, 邱红波. 玉米第8染色体单片段代换系的构建与灰斑病抗性材料筛选[J]. 浙江农业学报, 2021, 33(3): 383-389.

YANG Mei, HU Xiaolan, SHEN Tao, TAN Kang, LIU Dailing, QIU Hongbo. Construction of single fragment substitution lines of maize 8th chromosome and sreening of resistant maize germplasm to gray leaf spot[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 383-389.

图/表 5

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染色体片段代换 CSILs	病级Rating									抗性评价 Resistance
	2018年 2018 year				2019年2019 year				平均 Average
	勐嘎Mengga		江东Jiangdong		勐嘎Mengga		江东Jiangdong
	标准差s	变异范围 Range of variation	标准差s	变异范围 Range of variation	标准差s	变异范围 Range of variation	标准差s	变异范围 Range of variation
J51	0	9~9	0	9~9	0	9~9	0	9~9	9.00	HS
T32	0	1~1	0	1~1	0	1~1	0	1~1	1.00	HR
H1	1.00	5~7	1.34	5~7	0.98	5~7	0.98	5~7	5.90	MR
H2	1.00	7~9	1.33	5~9	0.92	7~9	0	9~9	8.30	HS
H3	1.08	1~5	0.80	1~3	1.50	1~3	1.40	1~7	2.85	R
H4	1.00	7~9	0.98	7~9	0.80	7~9	0.98	7~9	7.85	HS
H5	1.20	5~9	0.60	7~9	0.80	7~9	0.92	7~9	8.60	HS
H6	0.60	7~9	0.60	7~9	0.80	7~9	0.92	7~9	8.15	HS
H7	1.08	3~7	0.98	5~7	1.00	5~9	0.98	5~7	5.80	MR
H8	0.80	7~9	0	9~9	0	9~9	0.60	7~9	8.85	HS
H9	1.00	7~9	0.98	7~9	0.98	7~9	0.98	7~9	8.05	HS
H10	0.92	7~9	0	9~9	0.60	7~9	0.80	7~9	8.70	HS
H11	0.98	7~9	0.80	7~9	0.98	7~9	0.98	7~9	8.30	HS
H12	0	9~9	0.92	7~9	0.98	7~9	0.98	7~9	8.25	HS
H13	0.92	7~9	0.60	7~9	0.80	7~9	0.80	7~9	8.60	HS
H14	0.80	7~9	1.28	5~9	0.92	7~9	0.60	7~9	7.95	HS
H15	1.00	7~9	0.98	7~9	0.98	7~9	0.92	7~9	8.20	HS
H16	1.28	5~9	1.00	7~9	0.98	7~9	0.98	7~9	7.80	HS
H17	0.60	1~3	0.60	1~3	0	3~3	0	3~3	2.90	R
H18	0.60	7~9	0	7~7	1.20	5~9	0.98	5~7	6.75	S
H19	1.33	5~9	0.80	7~9	1.00	7~9	0.92	7~9	8.20	HS
H20	0.92	7~9	0.00	9~9	0.92	7~9	0.60	7~9	8.05	HS
H21	0.98	7~9	0.80	7~9	0.92	7~9	1.00	7~9	8.20	HS
H22	0.60	7~9	0.92	7~9	0.80	7~9	0.98	7~9	8.50	HS
H23	0.60	7~9	0.80	7~9	0.60	7~9	0.92	7~9	8.65	HS

染色体片段代换 CSILs	病级Rating									抗性评价 Resistance
	2018年 2018 year				2019年2019 year				平均 Average
	勐嘎Mengga		江东Jiangdong		勐嘎Mengga		江东Jiangdong
	标准差s	变异范围 Range of variation	标准差s	变异范围 Range of variation	标准差s	变异范围 Range of variation	标准差s	变异范围 Range of variation
J51	0	9~9	0	9~9	0	9~9	0	9~9	9.00	HS
T32	0	1~1	0	1~1	0	1~1	0	1~1	1.00	HR
H1	1.00	5~7	1.34	5~7	0.98	5~7	0.98	5~7	5.90	MR
H2	1.00	7~9	1.33	5~9	0.92	7~9	0	9~9	8.30	HS
H3	1.08	1~5	0.80	1~3	1.50	1~3	1.40	1~7	2.85	R
H4	1.00	7~9	0.98	7~9	0.80	7~9	0.98	7~9	7.85	HS
H5	1.20	5~9	0.60	7~9	0.80	7~9	0.92	7~9	8.60	HS
H6	0.60	7~9	0.60	7~9	0.80	7~9	0.92	7~9	8.15	HS
H7	1.08	3~7	0.98	5~7	1.00	5~9	0.98	5~7	5.80	MR
H8	0.80	7~9	0	9~9	0	9~9	0.60	7~9	8.85	HS
H9	1.00	7~9	0.98	7~9	0.98	7~9	0.98	7~9	8.05	HS
H10	0.92	7~9	0	9~9	0.60	7~9	0.80	7~9	8.70	HS
H11	0.98	7~9	0.80	7~9	0.98	7~9	0.98	7~9	8.30	HS
H12	0	9~9	0.92	7~9	0.98	7~9	0.98	7~9	8.25	HS
H13	0.92	7~9	0.60	7~9	0.80	7~9	0.80	7~9	8.60	HS
H14	0.80	7~9	1.28	5~9	0.92	7~9	0.60	7~9	7.95	HS
H15	1.00	7~9	0.98	7~9	0.98	7~9	0.92	7~9	8.20	HS
H16	1.28	5~9	1.00	7~9	0.98	7~9	0.98	7~9	7.80	HS
H17	0.60	1~3	0.60	1~3	0	3~3	0	3~3	2.90	R
H18	0.60	7~9	0	7~7	1.20	5~9	0.98	5~7	6.75	S
H19	1.33	5~9	0.80	7~9	1.00	7~9	0.92	7~9	8.20	HS
H20	0.92	7~9	0.00	9~9	0.92	7~9	0.60	7~9	8.05	HS
H21	0.98	7~9	0.80	7~9	0.92	7~9	1.00	7~9	8.20	HS
H22	0.60	7~9	0.92	7~9	0.80	7~9	0.98	7~9	8.50	HS
H23	0.60	7~9	0.80	7~9	0.60	7~9	0.92	7~9	8.65	HS

株系编号 Lines number	供体片段位点 Donor fragment site	最短长度 Minimum length/cM	最长长度 Maximum length/cM	估计长度 Estimated length/cM
H1	umc2356	0	63.20	31.60
H2	umc2356-bnlg1607-umc1607-umc266d-bnlg1828	93.90	165.50	129.70
H3	umc1034-csu329	1.87	62.40	32.13
H4	umc1487	0	67.99	33.00
H5	umc2354	0	39.90	19.95
H6	umc1130-umc2367	14.40	51.60	33.00
H7	umc2218	0	64.70	32.35
H8	umc226d	0	40.30	20.15
H9	umc1414	0	80.50	40.25
H10	umc2352a-bnlg1073-umc1304	0	21.30	10.65
H11	bnlg1828-umc1807	14.80	36.81	25.81
H12	bnlg1828	0	31.01	15.51
H13	bnlg2046	0	25.90	12.95
H14	umc2354-bnlg1863-bnlg1460-umc1302	44.20	62.58	53.39
H15	phi080	0	24.30	12.15
H16	bnlg1607-umc1607	21.60	77.73	49.67
H17	umc1034-csu329-bnlg1828-umc1807-umc2354	38.68	87.60	63.14
H18	umc1384	0	56.67	28.34
H19	bnlg1828-umc1807-umc1377-bnlg1460	29.68	88.00	58.84
H20	umc1139	0	26.80	13.40
H21	umc2367-bnlg2181-umc1889-umc1316	37.20	82.80	60.00
H22	umc1807-umc2354-bnlg1863-bnlg1828-umc1807-umc1377	54.60	84.38	69.49
H23	umc1377	0	10.40	5.20

株系编号 Lines number	供体片段位点 Donor fragment site	最短长度 Minimum length/cM	最长长度 Maximum length/cM	估计长度 Estimated length/cM
H1	umc2356	0	63.20	31.60
H2	umc2356-bnlg1607-umc1607-umc266d-bnlg1828	93.90	165.50	129.70
H3	umc1034-csu329	1.87	62.40	32.13
H4	umc1487	0	67.99	33.00
H5	umc2354	0	39.90	19.95
H6	umc1130-umc2367	14.40	51.60	33.00
H7	umc2218	0	64.70	32.35
H8	umc226d	0	40.30	20.15
H9	umc1414	0	80.50	40.25
H10	umc2352a-bnlg1073-umc1304	0	21.30	10.65
H11	bnlg1828-umc1807	14.80	36.81	25.81
H12	bnlg1828	0	31.01	15.51
H13	bnlg2046	0	25.90	12.95
H14	umc2354-bnlg1863-bnlg1460-umc1302	44.20	62.58	53.39
H15	phi080	0	24.30	12.15
H16	bnlg1607-umc1607	21.60	77.73	49.67
H17	umc1034-csu329-bnlg1828-umc1807-umc2354	38.68	87.60	63.14
H18	umc1384	0	56.67	28.34
H19	bnlg1828-umc1807-umc1377-bnlg1460	29.68	88.00	58.84
H20	umc1139	0	26.80	13.40
H21	umc2367-bnlg2181-umc1889-umc1316	37.20	82.80	60.00
H22	umc1807-umc2354-bnlg1863-bnlg1828-umc1807-umc1377	54.60	84.38	69.49
H23	umc1377	0	10.40	5.20

玉米第8染色体单片段代换系的构建与灰斑病抗性材料筛选

Construction of single fragment substitution lines of maize 8th chromosome and sreening of resistant maize germplasm to gray leaf spot

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