生物炭对小麦根际和根内微生物群落结构的影响

doi:10.3969/j.issn.1004-1524.2021.03.17

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (3): 516-525.DOI: 10.3969/j.issn.1004-1524.2021.03.17

生物炭对小麦根际和根内微生物群落结构的影响

徐民民¹, 黄莹¹, 李波¹, 徐艳², 张帅², 姚岭芸², 王政²

1. 山东省环境保护科学研究设计院有限公司,山东济南 250100
2.青岛大学环境科学与工程学院,山东青岛 266071

收稿日期:2020-08-17 出版日期:2021-03-25 发布日期:2021-03-25
作者简介:徐民民(1987—),女,山东德州人,硕士,主要从事土壤微生物修复研究。E-mail: 046186@163.com
基金资助:
山东省自然科学基金(ZR2019YQ18);山东省高等学校青创人才引育计划(DC2000000961)

Effect of biochar on wheat root-associated microbial community structures

XU Minmin¹, HUANG Ying¹, LI Bo¹, XU Yan², ZHANG Shuai², YAO Lingyun², WANG Zheng²

1. Shandong Academy of Environmental Sciences Co., Ltd., Jinan 250100, China
2. College of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China

Received:2020-08-17 Online:2021-03-25 Published:2021-03-25

摘要/Abstract

摘要：

为了研究生物炭对小麦根际和根内微生物群落组成的影响,开展盆栽培养试验,利用高通量测序技术分析生物炭对小麦根内、根际和空白土壤中细菌群落结构组成的影响。结果表明,小麦根内微生物的群落多样性显著(P<0.05)低于根际和空白土壤中的微生物群落多样性,即植物根系能够过滤和筛选部分微生物在其根内定殖生长,其中,变形菌门(Proteobacteria)和蓝藻门(Cyanobacteria)是小麦根内环境中的优势菌门。生物炭能够显著(P<0.05)影响小麦根内和根际微生物组的结构组成。在根内,加生物炭显著(P<0.05)提高了疣微菌科(Verrucomicrobiaceae)和Luteolibacter属细菌的相对丰度;在根际,加生物炭条件下相对丰度显著(P<0.05)上升的微生物菌群包括疣微菌门(Verrucomicrobia)、厚壁菌门(Firmicutes)、芽单胞菌门(Gemmatimonadetes)、芽孢杆菌目(Bacillales)、环脂酸芽孢杆菌科(Alicyclobacillaceae)、Luteolibacter、Tumebacillus、芽单胞菌属(Gemmatimonas)、小坂菌属(Kosakonia)、溶杆菌属(Lysobacter)、假黄色单胞菌属(Pseudoxanthomonas)、Blastomonas、马赛菌属(Mssilia)、原囊菌属(Archangium)和肠杆菌属(Enterobacter)。整体来看,根际微生物群落对生物炭处理更为敏感,生物炭添加会显著(P<0.05)影响小麦根际的标志微生物种类。

关键词: 小麦, 生物炭, 高通量测序, 微生物多样性

Abstract:

In order to study the effect of biochar on the wheat root-associated microbiome, greenhouse experiment was conducted and the microbial communities in wheat endosphere, rhizosphere and bulk soil were sequenced by high throughput sequencing technology. The results showed that the microbial community diversity in endosphere was significantly (P<0.05) lower than that in the rhizosphere and bulk soil. In other words, plant roots could filter and screen some microorganisms to colonize and grow in the roots, among which Proteobacteria and Cyanobacteria were the dominant bacteria. Biochar could influence the structure and composition of wheat root-associated microbial community. In the endosphere, the relative abundance of Verrucomicrobiaceae and Luteolibacter was significantly (P<0.05) increased with biochar addition; while in the rhizosphere, biochar addition significantly (P<0.05) increased the relative abundance of Verrucomicrobia, Firmicutes, Gemmatimonadetes, Bacillales, Alicyclobacillaceae, Luteolibacter, Tumebacillus, Gemmatimonas, Kosakonia, Lysobacter, Pseudoxanthomonas, Blastomonas, Mssilia, Archangium and Enterobacter. The microbial communities in the rhizosphere was more sensitive to biochar, and the addition of biochar significantly (P<0.05) changed the biomarkers in the rhizosphere of wheat.

Key words: wheat, biochar, high throughput sequencing, microbial diversity

中图分类号:

S154.3

徐民民, 黄莹, 李波, 徐艳, 张帅, 姚岭芸, 王政. 生物炭对小麦根际和根内微生物群落结构的影响[J]. 浙江农业学报, 2021, 33(3): 516-525.

XU Minmin, HUANG Ying, LI Bo, XU Yan, ZHANG Shuai, YAO Lingyun, WANG Zheng. Effect of biochar on wheat root-associated microbial community structures[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 516-525.

图/表 6

图1 不同处理微生物群落组成的Venn图 E,不加生物炭的根内微生物;R,不加生物炭的根际微生物;S,不加生物炭的空白土壤微生物;EB,加生物炭的根内微生物;RB,加生物炭的根际微生物;SB,加生物炭的空白土壤微生物。下同。a,不加生物炭处理下根内、根际和空白土壤的微生物对比;b,加生物炭处理下根内、根际和空白土壤的微生物对比;c,不加生物炭和加生物炭处理的根内微生物对比;d,不加生物炭和加生物炭处理的根际微生物对比;e,不加生物炭和加生物炭处理的空白土壤微生物对比。

Fig.1 Venn diagram of microbial community composition under different treatments E, Microbiome in endosphere without biochar addition; R, Microbiome in rhizosphere without biochar addition; S, Microbiome in bulk soils without biochar addition; EB, Microbiome in endosphere with biochar addition; RB, Microbiome in rhizosphere with biochar addition; SB, Microbiome in bulk soils with biochar addition. The same as below. a, Comparison of microbiome without biochar addition; b, Comparison of microbiome with biochar addition; c, Comparison of microbiome in endosphere under different treatments; d, Comparison of microbiome in rhizosphere under different treatments; e, Comparison of microbiome in bulk soils under different treatments.

图2 不同处理的Shannon指数柱上无相同字母的表示处理间差异显著(P<0.05)。

Fig.2 Shannon index under different treatments Bars marked without the same letters indicated significant difference at P<0.05.

图3 全部处理下根内、根际和空白土壤中的微生物群落PCoA分析

Fig.3 PCoA analysis of microbial community in endosphere, rhizosphere and bunk soil under all treatments

图4 不加生物炭(a)和加生物炭(b)处理下主要菌门的相对丰度

Fig.4 Relative abundance of main phyla under treatments without (a) or with (b) biochar addition

图5 属水平上主要细菌的菌属分析对相对丰度值取常用对数。

Fig.5 Analysis of main microbial community composition at genus level The relative abundance was calculated by common logarithm.

图6 加与不加生物炭条件下小麦根内(a)和根际(b)环境中微生物种类的变化 LDA值为取常用对数后的结果。

Fig.6 Comparison of microbial community in endosphere (a) and rhizosphere (b) under treatments with or without biochar addition LDA score was the result after common logarithm.

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生物炭对小麦根际和根内微生物群落结构的影响

Effect of biochar on wheat root-associated microbial community structures

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