关键词: miR828, 靶基因, 生物信息学

Abstract:

MicroRNAs (miRNAs) are a class of small regulatory RNA. miRNAs play an important role in many natural and pathological processes in organisms. In order to study the characteristics and biological functions of plant miR828 family, the secondary structure, target gene prediction, sequence alignment and evolution tree of miR828 members were analyzed using bioinformatics method. The precursors and mature sequence of miR828 family members were obtained from the PmiREN database, and the secondary structure was predicted online through the precursor sequence, and the sequence comparison, target gene prediction and evolution tree were constructed through the mature sequence. A total of 45 mature miR828 members were obtained from the database, which only distributed in angiosperms. Precursor and mature miRNAs are highly conserved in plant evolution. The secondary structure prediction found that all members of the miR828 family could form stable stem ring structures. Target gene prediction analysis found that miR828 family target gene include domain proteins, transcription factors, carrier proteins, ubiquitin ligases, etc. Most of them are MYB transcription factors and MYB domain proteins. The phylogenetic tree analysis showed that the members of miR828 family were close to each other. These results provide a basis for further study on the characteristics and functions of plant miR828 family.

Key words: miR828, target gene, bioinformatics