›› 2013, Vol. 25 ›› Issue (1): 0-20.

• 论文 •    

棉花水通道蛋白序列生物信息学初步分析

王为1,2,王长彪3, 陈浩东1,4,刘方1,王春英1,张香娣1,王玉红1,王坤波1,*   

  1. 1中国农业科学院 棉花研究所, 棉花生物学国家重点实验室,河南 安阳 455000;2 江苏沿海地区农业科学研究所, 农业部沿海盐碱地科学观测实验站,江苏 盐城 223802;3山西省农业科学院 棉花研究所, 山西 运城 043800; 4湖南省棉花科学研究所 国家杂交棉研究推广中心,湖南 常德 415101
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-01-25 发布日期:2013-01-25

Preliminary bioinformatics analysis of aquaporin sequences available in cotton

WANG Wei;WANG Changbiao;CHEN Haodong;LIU Fang;WANG Chunying;ZHANG Xiangdi;WANG Yyuhong;WANG Kunbo;*   

  1. 1 Cotton Research Institute, Chinese Academy of Agricultural Sciences, State Key Laboratoy of Cotton Biology, China, Anyang 455000, China; 2 Agricultural Science Institute of Coastal Region of Jiangsu, Observation and Experimental Station of Saline Land of Coastal Area, Ministry of Agriculture, Yancheng 224002, China;3 Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China; 4 Cotton Sciences Research Institute of Hunan, National Hybrid Cotton Research Promotion Center, Changde 415101, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-01-25 Published:2013-01-25

摘要: 水通道蛋白(aquaporin,AQP)是高效转运水分子的膜内在蛋白,在调控植物的水分关系中有重要作用,和抗旱性能紧密相关。为初步评价分析棉花AQP序列资源,对网上最新且仅有的52条棉花AQP序列进行分子聚类,同时将其进行多序列比对及亚细胞定位,结果表明:棉花AQP大致分为7类,具有丰富的多样性,AQP基因在跨膜转运以及光合中起重要作用。另外,来自水稻、大豆、玉米、拟南芥的328 条AQP序列和52条棉花AQP序进行分子聚类,结果表明:有48条棉花AQP和大豆聚为一类,遗传距离较接近。

关键词: 棉花, 水通道蛋白, 耐盐, 抗旱, 分子聚类

Abstract: Aquaporin(AQP) is a kind of inner epicyte protein which efficiently transports water molecule relevant to drought resistance and plays an important role in water regulation. To evaluate and analyze cotton AQP sequence resources preliminarily, the latest 52 AQP sequences from cotton public database were selected and analyzed by molecular clustering,multi-sequence alignment and distribution and location of AQP genes in subtype cell. The results indicated that AQPs were roughly divided into seven categories with a rich diversity, and AQP genes played an important role in the transportation through membrane and photosynthesis. Besides, 328 AQP sequences from rice, soybean, maize, Arabidopsis and the latest online 52 AQP sequences available in cotton were carried out with molecular clustering. The results showed that 48 out of the 52 AQP sequences in cotton and soybean gathered for a clustering with a closer genetic distance.

Key words: cotton, aquaporin, salt tolerance, drought resistance, molecular clustering