白芨内生真菌的多样性

doi:10.3969/j.issn.1004-1524.2017.12.16

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (12): 2077-2083.DOI: 10.3969/j.issn.1004-1524.2017.12.16

白芨内生真菌的多样性

席刚俊¹, 李警保², 史俊¹, 韩正敏^{2, *}

1.江苏农林职业技术学院生物工程技术中心,江苏句容 211121;
2. 南京林业大学林学院,江苏南京 210037

收稿日期:2017-08-21 出版日期:2017-12-20 发布日期:2018-01-08
通讯作者: 韩正敏,E-mail:zmhan@njfu.edu.cn
作者简介:席刚俊(1981—),男,湖北仙桃人,硕士,助理研究员,从事铁皮石斛、白芨病害研究。E-mail:2469250487@qq.com
基金资助:
江苏省林业三新工程(LYSX[2015]02); 江苏农林职业技术学院院内项目(2017KJ31)

Diversity of endophytic fungi in Bletilla striata

XI Gangjun¹, LI Jingbao², SHI Jun¹, HAN Zhengmin^{2, *}

1. Bio-Engineering Center, Jiangsu Polytechnic College of Agriculture and Forestry, Jurong 211121, China;
2. College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Received:2017-08-21 Online:2017-12-20 Published:2018-01-08

摘要/Abstract

摘要： 为研究白芨内生菌的多样性,从新鲜白芨根中提取内生真菌的DNA,构建ITS rDNA文库,进行高通量测序和多项信息分析。结果显示,白芨内生真菌主要属于担子菌门(83.4%)和子囊菌门(14.3%),另外2.3%无法鉴定;担子菌门、子囊菌门、接合菌门的菌根优势度指数分别为0.834 1、0.142 6、0.000 3,无法鉴定的真菌菌根优势度指数为0.022 9。纲、目的分类水平上,伞菌纲蜡壳菌目占83.4%,盘菌纲盘菌目占11.4%,锤舌菌纲柔膜菌目占2.1%,无法鉴定的占2.3%。科分类水平上,内生真菌主要属于Serendipitacea(83.4%)和盘菌科(11.4%)。属的分类水平上,梨形孢属(Piriformospora)占83.4%。Shannon index、Chao1、Observed species和Simpson 4种曲线分析结果显示,白芨菌根内生真菌的多样性呈均匀分布。

关键词: 白芨, 内生真菌, 高通量测序, 分类水平, 多样性

Abstract: To research the diversity of endophytic fungi in Bletilla striata, DNA of endophytic fungi was extracted from the fresh root of Bletilla striata, and ITS rDNA library was constructed. Then, this library was analyzed by high-throughput Illumina sequencing technology and bioinformatics. The results showed that endophytic fungi of Bletilla striata were mainly distributed in Basidiomycota (83.4%) and Ascomycota (14.3%), the other 2.3% could't be identified. Dominance index of endophytic fungi were Basidiomycota (0.834 1), Ascomycota (0.142 6), Zygomycota (0.000 3), and unidentified (0.022 9). At Class and Order levels, the proportion of (Agaricomycetes) Sebacinales was 83.4%, (Discomycete) Pezizales was 11.4%, (Leotiomycetes) Helotiales was 2.1%, unidentified was 2.3%. At Family level, Serendipitacea was 83.4% and Pezizaceae was 11.4%. At Genus level, Piriformospora was 83.4%. Analysis of Shannon index, Chao1, Observed species and Simpson showed that diversity of endophytic fungi in Bletilla striata were uniformly distributed.

Key words: Bletilla striata, endophytic fungi, high-throughput sequencing, classification level, diversity

中图分类号:

S567.23⁺9

席刚俊, 李警保, 史俊, 韩正敏. 白芨内生真菌的多样性[J]. 浙江农业学报, 2017, 29(12): 2077-2083.

XI Gangjun, LI Jingbao, SHI Jun, HAN Zhengmin. Diversity of endophytic fungi in Bletilla striata[J]. , 2017, 29(12): 2077-2083.

参考文献

[1] CAMERON D D, LEAKE J R, READ D J. Mutualistic mycorrhiza in orchids: evidence from plant-fungus carbon and nitrogen transfers in the green-leaved terrestrial orchid Goodyera repens [J]. New Phytologist , 2006, 171(2): 405-416.
[2] WATERMAN R J, BIDARTONDO M I, STOFBERG J, et al. The effects of above-and belowground mutualisms on orchid speciation and coexistence [J]. American Naturalist , 2011, 177(2): 54-68.
[3] DEARNALEY J D. Further advances in orchid mycorrhizal research [J]. Mycorrhiza , 2007, 17(6): 475-486.
[4] DEARNALEY J D W, MARTOS F, SELOSSE M A. 12 Orchid Mycorrhizas: molecular ecology, physiology, evolution and conservation aspects [J]. Mycota , 2012 (9): 207-230.
[5] 范黎, 郭顺星. 兰科植物菌根真菌的研究进展[J].微生物学通报,1998,25(4):227-230.
FAN L, GUO S X. Research advances of orchid mycorrhizal fungi [J]. Microbiology China , 1998, 25(4): 227-230. (in Chinese)
[6] RASMUSSEN H N. Recent developments in the study of orchid Neottia nidusavis [J]. Plant and Soil , 2002, 244: 149-163.
[7] BOKULICH N A, MILLS D A. Improved selection of internal transcribed spacer-specific primers enables quantitative, ultra-high-throughput profiling of fungal communities [J]. Applied & Environmental Microbiology , 2013, 79(8): 2519-2526.
[8] ZHANG J, KOBERT K, FLOURI T, et al. PEAR: a fast and accurate Illumina Paired-End reAd merger [J]. Bioinformatics , 2014, 30(5): 614.
[9] ADLER C J, DOBNEY K, WEYRICH L S, et al. Sequencing ancient calcified dental plaque shows changes in oral microbiota with dietary shifts of the Neolithic and Industrial revolutions [J] . Nature Genetics , 2013, 45(4): 450-455.
[10] AHN J, SINHA R, PEI Z, et al. Human gut microbiome and risk for colorectal cancer [J]. Journal of the National Cancer Institute , 2013, 105(24):1907.
[11] CAPORASO J G, KUCZYNSKI J, STOMBAUGH J, et al. QIIME allows analysis of high-throughput community sequencing data[J]. Nature Methods , 2010, 7(5): 335.
[12] AMANN R, LUDWIG IW, SCHLEIFER K H. Phylogentic identification and in situ detection of individual microbialcells without cultivation [J]. Microbiological Reviews 1995, 59(1): 143-169.
[13] 陈泽斌,李冰,王定康,等.铁皮石斛叶片内生真菌多样性的研究[J]. 福建农业学报, 2015, 30(10): 978-983.
CHEN Z B, LI B, WANG D K, et al. Diversity of endophytic fungi in leaves of Dendrobium candidiun [J]. Fujian Journal of Agricultural Sciences , 2015, 30(10): 978-983. (in Chinese with English abstract)
[14] 陶刚. 中国贵州兰科植物白及内生真菌多样性及生态分布研究[D]. 武汉: 华中农业大学, 2009.
TAO G. Diversity and ecological distribution of endophytic fungi associated with Bletilla ochracea in Guizhou, China [D]. Wuhan: Huazhong Agricultural University, 2009.
[15] 袁宁. 白芨组织培养技术体系研究[D]. 成都: 西南交通大学, 2008.
YUAN N. Study on the technique system of micropropagation in Bletilla striata [D]. Chengdu: Southwest Jiaotong University, 2008.
[16] 陈晓芳, 刘准, 陶刚, 等. 兰科菌根真菌对黄花白及 Bletilla ochracea Schltr.种子萌发率的影响[J]. 西南农业学报, 2012,25(4):1393-1397.
CHEN X F, LIU Z, TAO G, et al. Study on seeds germination of Bletilla ochraces improved by mycorrhizal fungi of orchid [J]. Southwest China Journal of Agricultural Sciences , 2012, 25(4): 1393-1397. (in Chinese with English abstract)
[17] 苏子敬. 白芨内生菌的分离与鉴定[D]. 重庆: 重庆师范大学, 2015.
SU Z J. Isolation and identification of endophytic fungi in Bletilla striata [D]. Chongqing: Chongqing Normal University, 2015.
[18] 刘准,陶刚,刘作易,等.兰科植物黄花白及 Bletilla ochracea 内生真菌多样性分析[J]. 菌物学报, 2013, 32(5): 812-818.
LIU Z, TAO G, LIU Z Y, et al. Diversity analysis of endophytic fungi associated with Bletilla ochracea (Orchidaceae) [J]. Mycosystema , 2013, 32(5): 812-818. (in Chinese with English abstract)
[19] 席刚俊, 李警保, 韩正敏, 等. 白芨种子萌发形态学观察及组培快繁体系的建立[J]. 浙江农业科学, 2017, 58(8): 1383-1387.
XI G J, LI J B, HAN Z M, et al. Morphological observation on seeds germination and establishment of tissue culture and rapid propagation system of Bletilla ochraces [J]. Journal of Zhejiang Agricultural Sciences , 2017, 58(8): 1383-1387. (in Chinese)

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白芨内生真菌的多样性

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