浙江农业学报 ›› 2019, Vol. 31 ›› Issue (3): 345-354.DOI: 10.3969/j.issn.1004-1524.2019.03.01

• 作物科学 • 上一篇    下一篇

杜仲内生拮抗细菌DZSY21诱导玉米抗病基因表达变化的转录组学研究

王其, 陈小洁, 顾双月, 张欣悦, 杭天露, 丁婷*   

  1. 安徽农业大学 植物保护学院,安徽 合肥 230036
  • 收稿日期:2018-06-24 出版日期:2019-03-25 发布日期:2019-04-08
  • 通讯作者: *丁婷,E-mail: ting98@126.com
  • 作者简介:王其(1993—),男,安徽固镇人,硕士研究生,研究方向为植物病害生物防治。E-mail: 542637154@qq.com
  • 基金资助:
    国家重点研发计划(2017YFD0201106)

Transcriptome profiling of maize resistance gene in response to DZSY21 induction

WANG Qi, CHEN Xiaojie, GU Shuangyue, ZHANG Xinyue, HANG Tianlu, DING Ting*   

  1. College of Plant Protection, Anhui Agricultural University, Hefei 230036, China
  • Received:2018-06-24 Online:2019-03-25 Published:2019-04-08

摘要: 杜仲内生拮抗细菌DZSY21可在玉米中稳定定殖并增强玉米植株的抗病能力。试验从基因水平上对内生拮抗细菌DZSY21诱导玉米产生抗病性的分子机制进行预测,利用转录组测序技术对内生拮抗细菌DZSY21处理玉米后不同生长时间段叶片的总mRNA进行差异表达分析,以Fold change≥2且FDR<0.01为标准,挑选差异表达基因(DEGs)。结果显示:以处理0 h作为对照,处理12 h时有2 413个差异表达基因,其中上调基因1 278个,下调基因1 135个;处理24 h时有737个差异表达基因,其中上调基因538个,下调基因199个。在此基础上,根据DEGs功能注释分类,在差异表达基因中筛选与抗病相关的基因,最终获得267个抗病基因。处理12 h筛选得到218个基因,主要涉及脂质转移蛋白、MATE转运蛋白及LysM受体蛋白激酶等26个抗病途径;处理24 h获得71个基因,主要涉及异黄酮还原酶、纤维素合成酶、苯丙氨酸解氨酶、L-抗坏血酸过氧化物酶、GLK转录因子及ACC氧化酶等30个抗病途径,其中不同处理时间段内重复基因23个。上述结果表明,将杜仲内生拮抗细菌DZSY21引入玉米,可调节玉米叶片中抗病相关基因的表达,为进一步寻找抗病基因及其功能鉴定奠定了基础。

关键词: DZSY21, 玉米, 抗病基因, 转录组

Abstract: Endophytic bacteria DZSY21 can stably colonize in maize and enhance the maize resistance to disease. Therefore, this study predicted the molecular mechanism of maize disease resistance induced by endophytic bacteria DZSY21 at the genetic level. The transcriptome sequencing technology was used to analyze the differential expression pattern of total genes in different periods of maize leaves inoculated with DZSY21. Cufflinks software was used to process the data, and the fold change≥2 and FDR<0.01 were set as standards to select DEGs. The results showed that there were 2 413 DEGs after inoculated with DZSY21 at 12 h compared with the control group inoculated with DZSY21 at 0 h, including 1 278 up-regulated DEGs and 1 135 down-regulated DEGs. There were 737 DEGs after inoculation at 24 h, including 538 up-regulated DEGs and 199 down-regulated DEGs. According to the results of functional annotation, 218 DEGs were obtained involved in 26 resistance pathways after inoculation at 12 h, including bifunctional inhibitor/lipid-transfer protein, MATE efflux family protein and LysM domain receptor-like kinase. There were 71 DEGs involved in 30 resistance pathways after inoculation at 24 h, including isoflavone reductase, probable cellulose synthase, phenylalanine ammonia-lyase, L-ascorbate peroxidase, probable transcription factor GLK and ACC oxidase. There were 23 DEGs duplicated in different treatment periods. The above results showed that the introduction of endophytic bacteria DZSY21 into maize could regulate the expression of resistance-related genes in maize leaves. This work provided a theoretical basis for further searching for resistance genes and their function identification.

Key words: DZSY21, maize, resistance gene, transcriptome

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