浙江农业学报 ›› 2020, Vol. 32 ›› Issue (10): 1866-1872.DOI: 10.3969/j.issn.1004-1524.2020.10.16

• 农产品质量安全 • 上一篇    下一篇

响应面法优化针叶樱桃总黄酮的提取工艺及其抗氧化活性研究

杨志1, 李文义1,2,*, 高云涛1, 熊华斌1, 陈毅坚2, 杨慧娟3   

  1. 1.云南民族大学 云南省跨境民族地区生物质资源清洁利用国际联合研究中心,云南 昆明 650500;
    2.云南民族大学 民族药资源化学国家民委-教育部重点实验室,云南 昆明 650500;
    3.中国计量大学 标准化学院,浙江 杭州 310018
  • 收稿日期:2020-08-04 出版日期:2020-10-25 发布日期:2020-11-16
  • 通讯作者: *李文义,E-mail:ymu777@163.com
  • 作者简介:杨志(1986—),男,云南大理人,白族,博士研究生,讲师,研究方向为资源环境与食品功能抗氧化。E-mail:512403510@qq.com
  • 基金资助:
    云南省应用基础研究计划(2016FD057,2017FD117); 浙江绍兴上虞标准化研究院项目

Optimization of extraction process of total flavonoids from Acerola cherry by response surface methodology and their antioxidant activities

YANG Zhi1, LI Wenyi1,2,*, GAO Yuntao1, XIONG Huabin1, CHEN Yijian2, YANG Huijuan3   

  1. 1. Joint Research Centre for International Cross-Border Ethnic Regions Biomass Clean Utilization in Yunnan, Yunnan Minzu University, Kunming 650500, China;
    2. Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission and Ministry of Education, Yunnan Minzu University, Kunming 650500, China;
    3. College of Standardization, China Jiliang University, Hangzhou 310018, China
  • Received:2020-08-04 Online:2020-10-25 Published:2020-11-16

摘要: 为有效提取针叶樱桃中的总黄酮,在单因素实验基础上,采用响应面法研究樱桃破碎目数、乙醇体积分数、液料比对黄酮提取率的影响,并对提取的黄酮进行抗氧化活性评价。结果表明:最佳提取工艺为樱桃破碎目数63.13、乙醇体积分数49.71%、液料比29.85,此时黄酮提取率预测值为85.07 mg·100g-1,与验证实验值86.9 mg·100g-1相符,说明响应模型方法可靠;且提取率越高,提取液抗氧化活性越好,最佳提取工艺下的DPPH抗氧化当量为73.38。

关键词: 针叶樱桃, 总黄酮, 响应面法, 抗氧化活性

Abstract: For effective extraction of total flavonoids from Acerola cherry, on the basis of single factor experiments, the effect of cherry crushing mesh number, ethanol concentration, and liquid-solid ratio were studied by response surface methodology. Meanwhile, the antioxidant activity of total flavonoids from Acerola cherry was assessed. The results showed that the optimum extraction conditions were: cherry crushing mesh number of 63.13, ethanol volume fraction of 49.71%, liquid-solid ratio of 29.85. Under the optimal conditions, flavonoids extraction yield was 86.9 mg·100g-1 and was closed to the predicted value of 85.07 mg·100g-1. The antioxidant activity of the extracts increased with the increase of extraction rate, and the DPPH antioxidant equivalent under the optimum extraction conditions was 73.38.

Key words: Acerola cherry, total flavonoids, response surface methodology, antioxidant activity

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