浙江农业学报 ›› 2022, Vol. 34 ›› Issue (11): 2416-2427.DOI: 10.3969/j.issn.1004-1524.2022.11.11
浙江省地方菜豆种质资源鉴定与遗传多样性分析
汪宝根1(
), 董君暘2, 汪颖1, 李素娟3, 王尖1, 鲁忠富1, 吴晓花1, 李国景1, 吴新义1,3,*()
- 1.浙江省农业科学院 蔬菜研究所,农业农村部南方豆类蔬菜种质创新与分子育种重点实验室(部省共建),浙江 杭州 310021
2.浙江农林大学 园艺科学学院,浙江 杭州 311300
3.浙江省农业科学院 省部共建农产品质量安全危害因子与风险防控国家重点实验室,浙江 杭州 310021
-
收稿日期:2022-03-07出版日期:2022-11-25发布日期:2022-11-29 -
作者简介:*吴新义,E-mail: wuxinyi@zaas.ac.cn
汪宝根(1976—),男,浙江淳安人,学士,副研究员,研究方向为豇豆育种。E-mail: wangbg@zaas.ac.cn -
通讯作者:吴新义 -
基金资助:国家重点研发计划(2017YFE0114500)
Evaluation and genetic diversity analysis of common bean germplasm in Zhejiang Province, China
WANG Baogen1(), DONG Junyang2, WANG Ying1, LI Sujuan3, WANG Jian1, LU Zhongfu1, WU Xiaohua1, LI Guojing1, WU Xinyi1,3,*()
- 1. Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. College of Horticulture Science, Zhejiang A&F University, Hangzhou 310021, China
3. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
-
Received:2022-03-07Online:2022-11-25Published:2022-11-29 -
Contact:WU Xinyi
摘要：
为鉴定浙江省地方菜豆种质遗传多样性,对浙江省历年来收集到的44份菜豆种质进行13个重要农艺性状的田间调查。结果表明,浙江地方菜豆种质在生长习性、嫩荚商品性等性状上均存在丰富的遗传变异。从发表的菜豆BARCBean6K_3 BeadChip芯片中随机、均匀挑选了185个SNP转化成KASP标记,对44份种质进行基因型鉴定后获得了88个高质量SNP基因型,主成分分析和系统进化树分析发现这些种质可以分为2大类,亚群分化与千粒重高度相关,且浙江省菜豆种质以中美洲基因池为主。研究结果可进一步丰富我国菜豆遗传基础,为地方菜豆资源的高效利用和新品种选育提供科学依据,所开发的KASP标记也可为菜豆种质基础研究提供有力的遗传工具。
中图分类号:
引用本文
汪宝根, 董君暘, 汪颖, 李素娟, 王尖, 鲁忠富, 吴晓花, 李国景, 吴新义. 浙江省地方菜豆种质资源鉴定与遗传多样性分析[J]. 浙江农业学报, 2022, 34(11): 2416-2427.
WANG Baogen, DONG Junyang, WANG Ying, LI Sujuan, WANG Jian, LU Zhongfu, WU Xiaohua, LI Guojing, WU Xinyi. Evaluation and genetic diversity analysis of common bean germplasm in Zhejiang Province, China[J]. Acta Agriculturae Zhejiangensis, 2022, 34(11): 2416-2427.
表1 浙江省44份菜豆地方种质资源的基本信息
Table 1 Information of 44 common bean germplasms collected from Zhejiang province
| 编号 Code | 种质名称 Germplasm name | 收集地点 Collecting locations |
|---|---|---|
| CB01 | 王阜四季豆Wangfu common bean | 淳安县Chunan County |
| CB02 | 梅豆Plum bean | 建德市Jiande |
| CB03 | 四季豆 Common bean | 建德市Jiande |
| CB04 | 花籽扁梅豆Huazibian plum bean | 浦江县Pujiang County |
| CB05 | 土京豆Tujing bean | 永康市Yongkang |
| CB06 | 土京豆Tujing bean | 永康市Yongkang |
| CB07 | 仙居矮脚金豆 | 仙居县Xianju County |
| Xianjuaijiaojing bean | ||
| CB08 | 花梅豆Huamei baen | 建德市Jiande |
| CB09 | 四季豆Common bean | 庆元县Qingyuan County |
| CB10 | 四季豆Common bean | 景宁县Jingning County |
| CB11 | 四季豆Common bean | 庆元县Qingyuan County |
| CB12 | 四季豆Common bean | 庆元县Qingyuan County |
| CB13 | 四季豆Common bean | 庆元县Qingyuan County |
| CB14 | 四季豆Common bean | 开化县Kaihua County |
| CB15 | 四季豆Common bean | 开化县Kaihua County |
| CB16 | 四季豆Common bean | 衢州市Quzhou |
| CB17 | 四季豆Common bean | 衢州市Quzhou |
| CB18 | 兰溪白花白荚 | 兰溪市Lanxi |
| Lanxi Baihuabaijia bean | ||
| CB19 | 三都春分豆Sanduchunfen bean | 松阳县Songyang County |
| CB20 | 矮脚四季豆Aijiao common bean | 仙居县Xianju County |
| CB21 | 红毛四季豆 | 杭州市Hangzhou |
| Hongmao common bean | ||
| CB22 | 四季豆Common bean | 景宁县Jingning County |
| CB23 | 四季豆Common bean | 桐乡市Tongxiang |
| CB24 | 四季豆Common bean | 衢州市Quzhou |
| CB25 | 四季豆Common bean | 诸暨市Zhuji |
| CB26 | 扁荚Bianjia bean | 杭州市Hangzhou |
| CB27 | 四季豆Common bean | 开化县Kaihua County |
| CB28 | 四季豆Common bean | 杭州市Hangzhou |
| CB53 | 白银豆Baiying bean | 瑞安市Ruian |
| CB54 | 白籽梅豆Baizi plum bean | 宁波市 Ningbo |
| CB58 | 高温四季豆Gaowen common bean | 杭州市Hangzhou |
| CB59 | 杭州白花四季豆 | 杭州市Hangzhou |
| Hangzhou Baihua common bean | ||
| CB60 | 黑珍珠Heizhenzhu bean | 湖州市Huzhou |
| CB61 | 红花白荚Honghuabaijia bean | 泰顺县Taishun County |
| CB62 | 红花青荚Honghuaqingjia bean | 丽水市Lishui |
| CB63 | 红花四季豆Honghua common bean | 杭州市Hangzhou |
| CB64 | 红筋园荚金豆 | 仙居县Xianju County |
| Hongjingyuanjiajin bean | ||
| 编号 Code | 种质名称 Germplasm name | 收集地点 Collecting locations |
| CB65 | 花籽红花青荚 | 绍兴市Shaoxing |
| Hongzihonghuaqingjia bean | ||
| CB70 | 临安四季豆Linan common bean | 杭州市Hangzhou |
| CB73 | 满棚红Manshanhong bean | 淳安县Chunan County |
| CB74 | 磐安四季豆Panan common bean | 磐安县Panan County |
| CB78 | 五漱四季豆Wushu common bean | 永嘉县Yongjia County |
| CB79 | 五漱四季豆Wushu common bean | 永嘉县Yongjia County |
| CB80 | 筱村四季豆Xiaocun common bean | 泰顺县Taishun County |
表1 浙江省44份菜豆地方种质资源的基本信息
Table 1 Information of 44 common bean germplasms collected from Zhejiang province
| 编号 Code | 种质名称 Germplasm name | 收集地点 Collecting locations |
|---|---|---|
| CB01 | 王阜四季豆Wangfu common bean | 淳安县Chunan County |
| CB02 | 梅豆Plum bean | 建德市Jiande |
| CB03 | 四季豆 Common bean | 建德市Jiande |
| CB04 | 花籽扁梅豆Huazibian plum bean | 浦江县Pujiang County |
| CB05 | 土京豆Tujing bean | 永康市Yongkang |
| CB06 | 土京豆Tujing bean | 永康市Yongkang |
| CB07 | 仙居矮脚金豆 | 仙居县Xianju County |
| Xianjuaijiaojing bean | ||
| CB08 | 花梅豆Huamei baen | 建德市Jiande |
| CB09 | 四季豆Common bean | 庆元县Qingyuan County |
| CB10 | 四季豆Common bean | 景宁县Jingning County |
| CB11 | 四季豆Common bean | 庆元县Qingyuan County |
| CB12 | 四季豆Common bean | 庆元县Qingyuan County |
| CB13 | 四季豆Common bean | 庆元县Qingyuan County |
| CB14 | 四季豆Common bean | 开化县Kaihua County |
| CB15 | 四季豆Common bean | 开化县Kaihua County |
| CB16 | 四季豆Common bean | 衢州市Quzhou |
| CB17 | 四季豆Common bean | 衢州市Quzhou |
| CB18 | 兰溪白花白荚 | 兰溪市Lanxi |
| Lanxi Baihuabaijia bean | ||
| CB19 | 三都春分豆Sanduchunfen bean | 松阳县Songyang County |
| CB20 | 矮脚四季豆Aijiao common bean | 仙居县Xianju County |
| CB21 | 红毛四季豆 | 杭州市Hangzhou |
| Hongmao common bean | ||
| CB22 | 四季豆Common bean | 景宁县Jingning County |
| CB23 | 四季豆Common bean | 桐乡市Tongxiang |
| CB24 | 四季豆Common bean | 衢州市Quzhou |
| CB25 | 四季豆Common bean | 诸暨市Zhuji |
| CB26 | 扁荚Bianjia bean | 杭州市Hangzhou |
| CB27 | 四季豆Common bean | 开化县Kaihua County |
| CB28 | 四季豆Common bean | 杭州市Hangzhou |
| CB53 | 白银豆Baiying bean | 瑞安市Ruian |
| CB54 | 白籽梅豆Baizi plum bean | 宁波市 Ningbo |
| CB58 | 高温四季豆Gaowen common bean | 杭州市Hangzhou |
| CB59 | 杭州白花四季豆 | 杭州市Hangzhou |
| Hangzhou Baihua common bean | ||
| CB60 | 黑珍珠Heizhenzhu bean | 湖州市Huzhou |
| CB61 | 红花白荚Honghuabaijia bean | 泰顺县Taishun County |
| CB62 | 红花青荚Honghuaqingjia bean | 丽水市Lishui |
| CB63 | 红花四季豆Honghua common bean | 杭州市Hangzhou |
| CB64 | 红筋园荚金豆 | 仙居县Xianju County |
| Hongjingyuanjiajin bean | ||
| 编号 Code | 种质名称 Germplasm name | 收集地点 Collecting locations |
| CB65 | 花籽红花青荚 | 绍兴市Shaoxing |
| Hongzihonghuaqingjia bean | ||
| CB70 | 临安四季豆Linan common bean | 杭州市Hangzhou |
| CB73 | 满棚红Manshanhong bean | 淳安县Chunan County |
| CB74 | 磐安四季豆Panan common bean | 磐安县Panan County |
| CB78 | 五漱四季豆Wushu common bean | 永嘉县Yongjia County |
| CB79 | 五漱四季豆Wushu common bean | 永嘉县Yongjia County |
| CB80 | 筱村四季豆Xiaocun common bean | 泰顺县Taishun County |
图1 浙江省地方菜豆种质分布
Fig.1 Distribution of common bean germplasm in Zhejiang Province
图1 浙江省地方菜豆种质分布
Fig.1 Distribution of common bean germplasm in Zhejiang Province
图2 六份代表性种质不同生育期的照片
Fig.2 Photos of six representative common bean germplasms at different growth periods
图2 六份代表性种质不同生育期的照片
Fig.2 Photos of six representative common bean germplasms at different growth periods
表2 四十四份种质在5个农艺性状上的表现
Table 2 Performances of five agronomic traits in the 44 accessions
| 性状 Traits | 最小值 Minimum | 中位数 Median | 最大值 Maximum | 平均值±标准误 | 变异系数 Coefficient of variation |
|---|---|---|---|---|---|
| 始花节位 First flower node | 2.30 | 3.70 | 7.20 | 3.9±0.17 | 0.28 |
| 荚长 Pod length/cm | 11.80 | 15.70 | 21.30 | 16.01±0.36 | 0.15 |
| 荚宽 Pod length/cm | 0.74 | 1.09 | 1.73 | 1.06±0.03 | 0.18 |
| 单荚重 Single pod weight/g | 6.00 | 10.90 | 16.60 | 10.97±0.37 | 0.22 |
| 千粒重 Thousang seed weight/g | 144.56 | 260.23 | 528.00 | 271.59±10.41 | 0.25 |
表2 四十四份种质在5个农艺性状上的表现
Table 2 Performances of five agronomic traits in the 44 accessions
| 性状 Traits | 最小值 Minimum | 中位数 Median | 最大值 Maximum | 平均值±标准误 | 变异系数 Coefficient of variation |
|---|---|---|---|---|---|
| 始花节位 First flower node | 2.30 | 3.70 | 7.20 | 3.9±0.17 | 0.28 |
| 荚长 Pod length/cm | 11.80 | 15.70 | 21.30 | 16.01±0.36 | 0.15 |
| 荚宽 Pod length/cm | 0.74 | 1.09 | 1.73 | 1.06±0.03 | 0.18 |
| 单荚重 Single pod weight/g | 6.00 | 10.90 | 16.60 | 10.97±0.37 | 0.22 |
| 千粒重 Thousang seed weight/g | 144.56 | 260.23 | 528.00 | 271.59±10.41 | 0.25 |
图3 五个主要农艺性状在44份种质中的分布频率
Fig.3 Distribution frequencies of five traits of 44 common bean accessions
图3 五个主要农艺性状在44份种质中的分布频率
Fig.3 Distribution frequencies of five traits of 44 common bean accessions
图4 KASP标记在44份种质中的扩增信号 A,一种基因型;B,两种基因型。蓝色信号代表HEX等位变异,红色信号代表FAM等位变异。
Fig.4 Amplification signals for the KASP markers in 44 accessions A, Single genotype; B, Two genotypes. Blue signal represented HEX allelic variation, red signal represented FAM allelic variation.
图4 KASP标记在44份种质中的扩增信号 A,一种基因型;B,两种基因型。蓝色信号代表HEX等位变异,红色信号代表FAM等位变异。
Fig.4 Amplification signals for the KASP markers in 44 accessions A, Single genotype; B, Two genotypes. Blue signal represented HEX allelic variation, red signal represented FAM allelic variation.
表3 本研究开发的88对KASP标记基本信息
Table 3 Information of 88 KASP-SNP markers in this study
| ID | 染色体 Chromosome | 基因组位置 SNP position in chromosome v1.0 | FAM引物 Primer_AlleleFAM | HEX引物 Primer_AlleleHEX | 通用引物 Universal primer | FAM等 位变异 Allele_ FAM | HEX等 位变异 Allele_ HEX |
|---|---|---|---|---|---|---|---|
| 715645089 | Chr01 | 3044239 | GAAGGTGACCAAGTTCATGCTTTAAGATTCATGTGCATG | GAAGGTCGGAGTCAACGGATTTTAAGATTCATGTGCATT | GTTTGGTTCCTCTGAATTTATATTTATTTT | G | T |
| 715643138 | Chr01 | 24744217 | GAAGGTGACCAAGTTCATGCTGCAGTACTCACAACATCAATC | GAAGGTCGGAGTCAACGGATTGCAGTACTCACAACATCAATA | TCTCTAGAAGAGCTTGCAAGGACTATTATG | C | A |
| 715640479 | Chr01 | 35496757 | GAAGGTGACCAAGTTCATGCTGTCATAGAATATGCCGATG | GAAGGTCGGAGTCAACGGATTGTCATAGAATATGCCGATT | TATTCTAAAAGTATGTCTTGCA | G | T |
| 715639381 | Chr01 | 37227412 | GAAGGTGACCAAGTTCATGCTCAGGTTAGGGTTAGGGTTTGCTTT | GAAGGTCGGAGTCAACGGATTCAGGTTAGGGTTAGGGTTTGCTTC | GTCTCCGCGGACTTTCCGGGATC | T | C |
| 715648889 | Chr01 | 43699409 | GAAGGTGACCAAGTTCATGCTCTCAGTGTTCGTCATGTGCCATGTA | GAAGGTCGGAGTCAACGGATTCTCAGTGTTCGTCATGTGCCATGTG | ATGGGACTGAGCAGAAACCTTA | A | G |
| 715645921 | Chr01 | 48941111 | GAAGGTGACCAAGTTCATGCTCACCAATAGTTTCAAACTACTAT | GAAGGTCGGAGTCAACGGATTCACCAATAGTTTCAAACTACTAC | TGATAACGTGATGTGATCTAGGTAAC | T | C |
| 715649479 | Chr02 | 6743 | GAAGGTGACCAAGTTCATGCTGGTTTAACTGGGGTGTCTAGA | GAAGGTCGGAGTCAACGGATTGGTTTAACTGGGGTGTCTAGG | TGTCAATCTCCAATCCACCAAG | A | G |
| 715640819 | Chr02 | 5855171 | GAAGGTGACCAAGTTCATGCTGGTGGCGAATGAATTCAAATTGC | GAAGGTCGGAGTCAACGGATTGGTGGCGAATGAATTCAAATTGT | AAAGCTATACATGCCACTAAAGTTTTG | C | T |
| 715647790 | Chr02 | 8927833 | GAAGGTGACCAAGTTCATGCTGGTTCATGCTTTAGGCTTAG | GAAGGTCGGAGTCAACGGATTGGTTCATGCTTTAGGCTTAA | ACCACTAGTAAAATTCAATTTCAGTTTCTT | G | A |
| 715642024 | Chr02 | 12142956 | GAAGGTGACCAAGTTCATGCTGGCTTCGTTTGCATGAATGA | GAAGGTCGGAGTCAACGGATTGGCTTCGTTTGCATGAATGG | CACCTTAGGACCAAGTAAACACAAACACAA | A | G |
| 715641350 | Chr02 | 21494397 | GAAGGTGACCAAGTTCATGCTGACTTGGACATTATTCTTATGGATG | GAAGGTCGGAGTCAACGGATTGACTTGGACATTATTCTTATGGATA | ATGTTAACTTTTTAAAAAAAAACCTC | G | A |
| 715648528 | Chr02 | 24042084 | GAAGGTGACCAAGTTCATGCTAACTAAAGGGCCTCGACCCC | GAAGGTCGGAGTCAACGGATTAACTAAAGGGCCTCGACCCT | GTTTTCATGGCACCGAGGCGAATACGAAGA | C | T |
| 715647099 | Chr02 | 32859750 | GAAGGTGACCAAGTTCATGCTGGTAAGTTTGGTGCTATTGCTT | GAAGGTCGGAGTCAACGGATTGGTAAGTTTGGTGCTATTGCTC | TAACCTTTTTCCTCACGTTTCTT | T | C |
| 715646488 | Chr02 | 36676613 | GAAGGTGACCAAGTTCATGCTCCATCCTTAAGAACTTGTGCAT | GAAGGTCGGAGTCAACGGATTCCATCCTTAAGAACTTGTGCAC | ACACCTTCAATGCTGTGGTAACA | T | C |
| 715647680 | Chr02 | 43900872 | GAAGGTGACCAAGTTCATGCTCAAGCTCTGTCATGCTCATGC | GAAGGTCGGAGTCAACGGATTCAAGCTCTGTCATGCTCATGT | ATAGTCTAGACCACATCACACG | C | T |
| 715648447 | Chr02 | 48531519 | GAAGGTGACCAAGTTCATGCTGGTTGTCAAAGGTTTAAAGCTG | GAAGGTCGGAGTCAACGGATTGGTTGTCAAAGGTTTAAAGCTA | GATTCTTCAGTAAGAGTAACCATG | G | A |
| ID | 染色体 Chromosome | 基因组位置 SNP position in chromosome v1.0 | FAM引物 Primer_AlleleFAM | HEX引物 Primer_AlleleHEX | 通用引物 Universal primer | FAM等 位变异 Allele_ FAM | HEX等 位变异 Allele_ HEX |
| 715644947 | Chr03 | 6366468 | GAAGGTGACCAAGTTCATGCTCCGTGTCACTGAGATCACTATC | GAAGGTCGGAGTCAACGGATTCCGTGTCACTGAGATCACTATT | AATACGAGGATGAATACATTTC | C | T |
| 715647580 | Chr03 | 12483803 | GAAGGTGACCAAGTTCATGCTAAAAACCTATCAAAGTAG | GAAGGTCGGAGTCAACGGATTAAAAACCTATCAAAGTAA | CTTTGTTCTTTGTTTCTCACAT | G | A |
| 715642031 | Chr03 | 15073563 | GAAGGTGACCAAGTTCATGCTTAGTTTTCATATTCAACC | GAAGGTCGGAGTCAACGGATTTAGTTTTCATATTCAACT | ATGAGAAATCAACTGGATCAAA | C | T |
| 715643408 | Chr03 | 27104732 | GAAGGTGACCAAGTTCATGCTGTCAGACAAACTCATTTACCCCG | GAAGGTCGGAGTCAACGGATTGTCAGACAAACTCATTTACCCCA | ATAAATCATTCCACCATCTAGA | G | A |
| 715649919 | Chr03 | 30251280 | GAAGGTGACCAAGTTCATGCTGCATTAATAGAGGATAGATGAAAC | GAAGGTCGGAGTCAACGGATTGCATTAATAGAGGATAGATGAAAT | GTTGTCTCATTGATAACCCTCA | C | T |
| 715650183 | Chr03 | 39786455 | GAAGGTGACCAAGTTCATGCTGTTTGCCCTTCTATTGGTATA | GAAGGTCGGAGTCAACGGATTGTTTGCCCTTCTATTGGTATC | TCTCCAACAAGCAAGAGCACTA | A | C |
| 715649769 | Chr04 | 3592 | GAAGGTGACCAAGTTCATGCTGTGCAAACCTGCAACTCAAAATTGC | GAAGGTCGGAGTCAACGGATTGTGCAAACCTGCAACTCAAAATTGA | ATCAGAACTGGAAGGATATCTT | C | A |
| 715647290 | Chr04 | 3109241 | GAAGGTGACCAAGTTCATGCTCCCTCTCTACCTAAGAACATGA | GAAGGTCGGAGTCAACGGATTCCCTCTCTACCTAAGAACATGC | GTTTTATGGTTAAGTGGTTAGTTTTGTAAG | A | C |
| 715641595 | Chr04 | 6493282 | GAAGGTGACCAAGTTCATGCTCGATATATCACCCATTAAC | GAAGGTCGGAGTCAACGGATTCGATATATCACCCATTAAA | ACCTTATAGTGTTTTGTTATCA | C | A |
| 715642949 | Chr04 | 15465426 | GAAGGTGACCAAGTTCATGCTGACTTCCTTATGCAGATCAACAT | GAAGGTCGGAGTCAACGGATTGACTTCCTTATGCAGATCAACAC | GAGTTTGATGAAGTACTTCATAAC | T | C |
| 715650920 | Chr04 | 21774557 | GAAGGTGACCAAGTTCATGCTTCCCACACACACTCACATCCAACAC | GAAGGTCGGAGTCAACGGATTTCCCACACACACTCACATCCAACAT | TGCTTAAAAGGACCACCTCGCTTA | C | T |
| 715650671 | Chr04 | 25684069 | GAAGGTGACCAAGTTCATGCTTGTTAGAACGCTGGTGCAT | GAAGGTCGGAGTCAACGGATTTGTTAGAACGCTGGTGCAG | GAAAAGTAAAATCTGCATCACC | T | G |
| 715642248 | Chr04 | 34571194 | GAAGGTGACCAAGTTCATGCTTCCCTTAACTTACTTGTGG | GAAGGTCGGAGTCAACGGATTTCCCTTAACTTACTTGTGA | CTTTTCAGGCTTGCTTTGCAGAC | G | A |
| 715639507 | Chr04 | 37382175 | GAAGGTGACCAAGTTCATGCTGTCACGGTGAATATTACCCG | GAAGGTCGGAGTCAACGGATTGTCACGGTGAATATTACCCA | AGAAGGTCAGATAGATAGGTCC | G | A |
| 715646125 | Chr04 | 43085220 | GAAGGTGACCAAGTTCATGCTCCTAATAAGCTTAAGTCAATACCTG | GAAGGTCGGAGTCAACGGATTCCTAATAAGCTTAAGTCAATACCTT | AAAACTGAAAAGTGAAATGCTC | G | T |
| 715639543 | Chr05 | 6447545 | GAAGGTGACCAAGTTCATGCTGTCGATTGAAGGCTTTATCTCAT | GAAGGTCGGAGTCAACGGATTGTCGATTGAAGGCTTTATCTCAC | ACATTGAAATCAAACTCACACACTAA | T | C |
| 715644469 | Chr05 | 9344921 | GAAGGTGACCAAGTTCATGCTGAGTGTGCCCTAAAACAAAATATC | GAAGGTCGGAGTCAACGGATTGAGTGTGCCCTAAAACAAAATATA | CTTGTAGCAGTTTTCTATCACGAGAGTATC | C | A |
| 715645339 | Chr05 | 39192763 | GAAGGTGACCAAGTTCATGCTGCTGAGCTAAGACTATGAATCGGGC | GAAGGTCGGAGTCAACGGATTGCTGAGCTAAGACTATGAATCGGGT | TCCTTGTTAAATGTGCTCATTC | C | T |
| 715646737 | Chr05 | 40469914 | GAAGGTGACCAAGTTCATGCTACACACCCTTCTTTCTCTCCTCTCG | GAAGGTCGGAGTCAACGGATTACACACCCTTCTTTCTCTCCTCTCT | AGGACAACCACTCATTTTTGCTC | G | T |
| 715650987 | Chr06 | 15998 | GAAGGTGACCAAGTTCATGCTCCAAAGGAGTCCACATTCA | GAAGGTCGGAGTCAACGGATTCCAAAGGAGTCCACATTCC | GGCCTACTAGTTTTACAAGAATGTTC | A | C |
| 715647844 | Chr06 | 6504361 | GAAGGTGACCAAGTTCATGCTCTCTTCTAATTCAGGACCAAATC | GAAGGTCGGAGTCAACGGATTCTCTTCTAATTCAGGACCAAATT | CGAGGTAGGCAAGCCTTCCCTT | C | T |
| 715640066 | Chr06 | 9341450 | GAAGGTGACCAAGTTCATGCTGGGGCCAACCTCAAATTTA | GAAGGTCGGAGTCAACGGATTGGGGCCAACCTCAAATTTG | GTGTCCCCTTAATCATTTCACATTGTTC | A | G |
| 715645954 | Chr06 | 15786595 | GAAGGTGACCAAGTTCATGCTGACCTTCTGAAGGAACTGA | GAAGGTCGGAGTCAACGGATTGACCTTCTGAAGGAACTGG | ATGCAGGTAAAACCAGTGGTCCAGAC | A | G |
| 715650222 | Chr06 | 18501260 | GAAGGTGACCAAGTTCATGCTGTCACAATGAGGCCTTCTCTC | GAAGGTCGGAGTCAACGGATTGTCACAATGAGGCCTTCTCTT | ATCAGTATGATTCTACATGTTTTTGG | C | T |
| 715646818 | Chr06 | 21496816 | GAAGGTGACCAAGTTCATGCTAAATTACACTAGCATAAT | GAAGGTCGGAGTCAACGGATTAAATTACACTAGCATAAC | GAGCCATTTGTGTTTGAATATG | T | C |
| 715645677 | Chr06 | 27370471 | GAAGGTGACCAAGTTCATGCTGAATAAAGAGTGATGATA | GAAGGTCGGAGTCAACGGATTGAATAAAGAGTGATGATG | ACATGGCCATGTATTCCGTCTTGGTTTGGT | A | G |
| 715639203 | Chr06 | 30199530 | GAAGGTGACCAAGTTCATGCTGACATGTTCTTGCATTATGA | GAAGGTCGGAGTCAACGGATTGACATGTTCTTGCATTATGG | TGGTACCTTTGATGTATCACACT | A | G |
| 715639367 | Chr06 | 31688381 | GAAGGTGACCAAGTTCATGCTTCCTCTTCCTGCACCATAGTC | GAAGGTCGGAGTCAACGGATTTCCTCTTCCTGCACCATAGTT | CATGTGAGCAAGTGTTTGGTGGCATTGAAC | C | T |
| ID | 染色体 Chromosome | 基因组位置 SNP position in chromosome v1.0 | FAM引物 Primer_AlleleFAM | HEX引物 Primer_AlleleHEX | 通用引物 Universal primer | FAM等 位变异 Allele_ FAM | HEX等 位变异 Allele_ HEX |
| 715648393 | Chr07 | 61520 | GAAGGTGACCAAGTTCATGCTGTTGTCTTTCAATGAACGAT | GAAGGTCGGAGTCAACGGATTGTTGTCTTTCAATGAACGAC | AAGGCAGGAATCTTTAAAAGCT | T | C |
| 715645699 | Chr07 | 1153000 | GAAGGTGACCAAGTTCATGCTTGAGTTCACGTGCATTGGGCATG | GAAGGTCGGAGTCAACGGATTTGAGTTCACGTGCATTGGGCATT | CCAAACAACATAAAGCAATTTCTAACAAAC | G | T |
| 715648625 | Chr07 | 9516304 | GAAGGTGACCAAGTTCATGCTGCATGTAGTGTTTTTAGTTTC | GAAGGTCGGAGTCAACGGATTGCATGTAGTGTTTTTAGTTTT | CCATATCACTAGGACTCTGAAC | C | T |
| 715648880 | Chr07 | 12261154 | GAAGGTGACCAAGTTCATGCTGACATCATCTTTGCAGTGAAC | GAAGGTCGGAGTCAACGGATTGACATCATCTTTGCAGTGAAT | TAATGGTCTTGATAAGGAGAGC | C | T |
| 715650406 | Chr07 | 15316545 | GAAGGTGACCAAGTTCATGCTTGACATAAATTGTGAAAG | GAAGGTCGGAGTCAACGGATTTGACATAAATTGTGAAAA | AAAGAATGTGAAAGAAGTCCAC | G | A |
| 715642111 | Chr07 | 18479982 | GAAGGTGACCAAGTTCATGCTCCGTAAGCTGGGACTTAACAGAAAT | GAAGGTCGGAGTCAACGGATTCCGTAAGCTGGGACTTAACAGAAAG | CCTACATTTAGGGTCCTTGTGTTTTT | T | G |
| 715643102 | Chr07 | 24160043 | GAAGGTGACCAAGTTCATGCTAGCAAGGGCGGATCAAAGGA | GAAGGTCGGAGTCAACGGATTAGCAAGGGCGGATCAAAGGC | CCTCCCCTTGAGAATCCTCCTG | A | C |
| 715640138 | Chr07 | 30148305 | GAAGGTGACCAAGTTCATGCTCTGTTTGCTTCTGTTTACTGC | GAAGGTCGGAGTCAACGGATTCTGTTTGCTTCTGTTTACTGT | TCTCTTCCAGTTCTTCCGTGCTAAA | C | T |
| 715641587 | Chr07 | 33842222 | GAAGGTGACCAAGTTCATGCTGACCCACTTAATCCATCTAC | GAAGGTCGGAGTCAACGGATTGACCCACTTAATCCATCTAT | AGGACTCAGTAAGGTGGGGCTTTCTTCTTC | C | T |
| 715639238 | Chr07 | 42558166 | GAAGGTGACCAAGTTCATGCTATTGGATCAGGTGTGATGCCG | GAAGGTCGGAGTCAACGGATTATTGGATCAGGTGTGATGCCA | AGGGGTGATCATGACACGCTTAC | G | A |
| 715646608 | Chr07 | 45792744 | GAAGGTGACCAAGTTCATGCTGCTGATTCATCATGCTCAC | GAAGGTCGGAGTCAACGGATTGCTGATTCATCATGCTCAA | GAATCTAAACTTGAACCACGTGT | C | A |
| 715646685 | Chr08 | 129003 | GAAGGTGACCAAGTTCATGCTGGATTTGGGGGAGAAGAAG | GAAGGTCGGAGTCAACGGATTGGATTTGGGGGAGAAGAAA | AAATTTTTTTCTCACCCCCACA | G | A |
| 715645832 | Chr08 | 3020556 | GAAGGTGACCAAGTTCATGCTCTCATGTAAACAATTGAT | GAAGGTCGGAGTCAACGGATTCTCATGTAAACAATTGAC | TTGCACATGTAATGCTTGGAAG | T | C |
| 715649757 | Chr08 | 6483262 | GAAGGTGACCAAGTTCATGCTCAAGCCTAATGACCACTGACACACA | GAAGGTCGGAGTCAACGGATTCAAGCCTAATGACCACTGACACACC | TGAAGTATTTGCAGAAAATTGGAGAC | A | C |
| 715647414 | Chr08 | 9422765 | GAAGGTGACCAAGTTCATGCTCAACTGCGACAAGATGGTACT | GAAGGTCGGAGTCAACGGATTCAACTGCGACAAGATGGTACC | ATAAACAAAAAATAGTGTCTCG | T | C |
| 715648702 | Chr08 | 15144723 | GAAGGTGACCAAGTTCATGCTGTCTTCTTCTTCCTTTTCTATT | GAAGGTCGGAGTCAACGGATTGTCTTCTTCTTCCTTTTCTATC | TTGCATAAAACGCATTAAATGT | T | C |
| 715644789 | Chr08 | 24424087 | GAAGGTGACCAAGTTCATGCTGCCTTAGTTGACACCTCCATTT | GAAGGTCGGAGTCAACGGATTGCCTTAGTTGACACCTCCATTC | CTCTTCATCGTGTGATGGTTGG | T | C |
| 715639796 | Chr08 | 33391365 | GAAGGTGACCAAGTTCATGCTGAGGAAAGGAATGCTTTCAACTG | GAAGGTCGGAGTCAACGGATTGAGGAAAGGAATGCTTTCAACTA | GGTGGTCAAATTCAACTAGGATGGC | G | A |
| 715640428 | Chr08 | 36879821 | GAAGGTGACCAAGTTCATGCTGCTACAAACAAATCATGATGCATCC | GAAGGTCGGAGTCAACGGATTGCTACAAACAAATCATGATGCATCT | ACTCTGTCATGAACGCGGTCTT | C | T |
| 715650893 | Chr08 | 39711359 | GAAGGTGACCAAGTTCATGCTTCATATCTTCAAAAAACT | GAAGGTCGGAGTCAACGGATTTCATATCTTCAAAAAACC | AGTCACATCCCATCAAATTCGG | T | C |
| 715648689 | Chr08 | 48679942 | GAAGGTGACCAAGTTCATGCTTATTTGCATGGCCCGACCAACCTC | GAAGGTCGGAGTCAACGGATTTATTTGCATGGCCCGACCAACCTT | CTTTAGTTTAGTAGGTTAAGTAAT | C | T |
| 715639302 | Chr08 | 51313104 | GAAGGTGACCAAGTTCATGCTTCTCTAACTCGTGCGTACG | GAAGGTCGGAGTCAACGGATTTCTCTAACTCGTGCGTACA | GTTTGTTGGGTTTATTATCGGA | G | A |
| 715650660 | Chr08 | 59634577 | GAAGGTGACCAAGTTCATGCTACATGATAAACACAAAAA | GAAGGTCGGAGTCAACGGATTACATGATAAACACAAAAG | TAGCCATATTGTTCTTCTATAAAAAAGCAG | A | G |
| 715650429 | Chr09 | 298696 | GAAGGTGACCAAGTTCATGCTCATTTGGTACCTCGTCTCAG | GAAGGTCGGAGTCAACGGATTCATTTGGTACCTCGTCTCAA | ACAAGAACTGGAACTAGTATAC | G | A |
| 715639476 | Chr09 | 3133765 | GAAGGTGACCAAGTTCATGCTCCTCTTACTCAAGAAAGCCAAGAT | GAAGGTCGGAGTCAACGGATTCCTCTTACTCAAGAAAGCCAAGAC | TCTATCTATCACGGATAAGTTG | T | C |
| 715639339 | Chr09 | 12175377 | GAAGGTGACCAAGTTCATGCTGCTTATAGTGTGGAGAGCAG | GAAGGTCGGAGTCAACGGATTGCTTATAGTGTGGAGAGCAA | GATTTCTCAATAGAAACATGCC | G | A |
| 715646043 | Chr09 | 21807115 | GAAGGTGACCAAGTTCATGCTGTGAGTACTAAGATTTGTGGTCTGG | GAAGGTCGGAGTCAACGGATTGTGAGTACTAAGATTTGTGGTCTGA | CGATAAGAACATTTGACACCCACTGT | G | A |
| 715646065 | Chr09 | 24807436 | GAAGGTGACCAAGTTCATGCTCCCTTGTGAGAAAGTATTGATTGTG | GAAGGTCGGAGTCAACGGATTCCCTTGTGAGAAAGTATTGATTGTA | GCCAATTTAGAAAGTTCTCCATGT | G | A |
| ID | 染色体 Chromosome | 基因组位置 SNP position in chromosome v1.0 | FAM引物 Primer_AlleleFAM | HEX引物 Primer_AlleleHEX | 通用引物 Universal primer | FAM等 位变异 Allele_ FAM | HEX等 位变异 Allele_ HEX |
| 715643007 | Chr10 | 95419 | GAAGGTGACCAAGTTCATGCTAACAATACTACGTTTAAT | GAAGGTCGGAGTCAACGGATTAACAATACTACGTTTAAC | GTGGACTGAGCCCTGTTAACTGGAAGCTAA | T | C |
| 715650121 | Chr10 | 6367592 | GAAGGTGACCAAGTTCATGCTTATTAAGGGAAAGATCGAC | GAAGGTCGGAGTCAACGGATTTATTAAGGGAAAGATCGAT | TGGACCCATGACCACAAGTTAAGAGCCTTC | C | T |
| 715641901 | Chr10 | 18965279 | GAAGGTGACCAAGTTCATGCTCCCCTAGTAGCCTTATACCAAC | GAAGGTCGGAGTCAACGGATTCCCCTAGTAGCCTTATACCAAT | TATTGGTAAGCCTGTTGAGGCA | C | T |
| 715644023 | Chr10 | 27898392 | GAAGGTGACCAAGTTCATGCTGAGCATCGTTTGGTAGAGCACAAGT | GAAGGTCGGAGTCAACGGATTGAGCATCGTTTGGTAGAGCACAAGG | GGCGCCCACCGTGGGGTTCGAA | T | G |
| 715640671 | Chr10 | 30260059 | GAAGGTGACCAAGTTCATGCTAGAGATTGGATGCATGAGTCGCCA | GAAGGTCGGAGTCAACGGATTAGAGATTGGATGCATGAGTCGCCG | GTGCGTAAGGCTGCCCTACCGAGG | A | G |
| 715639617 | Chr10 | 33411083 | GAAGGTGACCAAGTTCATGCTGCCTCATGCTTTATTGACAGGGC | GAAGGTCGGAGTCAACGGATTGCCTCATGCTTTATTGACAGGGT | ACACTTGTTTCTTTGCATCATGGA | C | T |
| 715640169 | Chr10 | 36497500 | GAAGGTGACCAAGTTCATGCTCACCTTGCCAGAATACTTCACCT | GAAGGTCGGAGTCAACGGATTCACCTTGCCAGAATACTTCACCC | CTGAGGATCCTGGGAAGCATTG | T | C |
| 715639540 | Chr10 | 42706527 | GAAGGTGACCAAGTTCATGCTGCTCACATCTTACTCATACCAACTG | GAAGGTCGGAGTCAACGGATTGCTCACATCTTACTCATACCAACTA | AACAATGGATGTGGTGACTTATCA | G | A |
| 715641318 | Chr11 | 16520 | GAAGGTGACCAAGTTCATGCTAAATACTTTCAACGTTTG | GAAGGTCGGAGTCAACGGATTAAATACTTTCAACGTTTA | AACCCTCTCGTGTGAAATTTCCACTATGAC | G | A |
| 715645562 | Chr11 | 3190094 | GAAGGTGACCAAGTTCATGCTTTTGATGAATGAATTTCG | GAAGGTCGGAGTCAACGGATTTTTGATGAATGAATTTCA | GTTTATATTGGAGCCTGCATGTGTTAGCTA | G | A |
| 715650149 | Chr11 | 6641086 | GAAGGTGACCAAGTTCATGCTGACCCAGCAGCTATGTCTGTTTTA | GAAGGTCGGAGTCAACGGATTGACCCAGCAGCTATGTCTGTTTTG | AGAATGCATAGCACTTATTTGATTGTG | A | G |
| 715639655 | Chr11 | 12487977 | GAAGGTGACCAAGTTCATGCTTCTCACCACCTGTACTCCAAACCTA | GAAGGTCGGAGTCAACGGATTTCTCACCACCTGTACTCCAAACCTG | TCGACATTAAACAATACCAAGGAGAATCC | A | G |
| 715644528 | Chr11 | 21570007 | GAAGGTGACCAAGTTCATGCTCCAGCTACACCTCAATAGCTAGA | GAAGGTCGGAGTCAACGGATTCCAGCTACACCTCAATAGCTAGG | GAAGGAGGCTGGCCAAAGTACCCACA | A | G |
| 715644319 | Chr11 | 24580005 | GAAGGTGACCAAGTTCATGCTGGAAGGTACAAGGGACGAGTATTTG | GAAGGTCGGAGTCAACGGATTGGAAGGTACAAGGGACGAGTATTTA | AACGCCCGAGGACGAGACGGTACACTAA | G | A |
| 715641793 | Chr11 | 33339240 | GAAGGTGACCAAGTTCATGCTTTTGAAGTGGGGGTTTGGAGTCCG | GAAGGTCGGAGTCAACGGATTTTTGAAGTGGGGGTTTGGAGTCCA | GCCAATAAGAAACATCCCGACC | G | A |
| 715648007 | Chr11 | 42300383 | GAAGGTGACCAAGTTCATGCTCAAACAATAGGAGAATAGCATT | GAAGGTCGGAGTCAACGGATTCAAACAATAGGAGAATAGCATC | TGAACCTTTTAACACCTCCTTA | T | C |
表3 本研究开发的88对KASP标记基本信息
Table 3 Information of 88 KASP-SNP markers in this study
| ID | 染色体 Chromosome | 基因组位置 SNP position in chromosome v1.0 | FAM引物 Primer_AlleleFAM | HEX引物 Primer_AlleleHEX | 通用引物 Universal primer | FAM等 位变异 Allele_ FAM | HEX等 位变异 Allele_ HEX |
|---|---|---|---|---|---|---|---|
| 715645089 | Chr01 | 3044239 | GAAGGTGACCAAGTTCATGCTTTAAGATTCATGTGCATG | GAAGGTCGGAGTCAACGGATTTTAAGATTCATGTGCATT | GTTTGGTTCCTCTGAATTTATATTTATTTT | G | T |
| 715643138 | Chr01 | 24744217 | GAAGGTGACCAAGTTCATGCTGCAGTACTCACAACATCAATC | GAAGGTCGGAGTCAACGGATTGCAGTACTCACAACATCAATA | TCTCTAGAAGAGCTTGCAAGGACTATTATG | C | A |
| 715640479 | Chr01 | 35496757 | GAAGGTGACCAAGTTCATGCTGTCATAGAATATGCCGATG | GAAGGTCGGAGTCAACGGATTGTCATAGAATATGCCGATT | TATTCTAAAAGTATGTCTTGCA | G | T |
| 715639381 | Chr01 | 37227412 | GAAGGTGACCAAGTTCATGCTCAGGTTAGGGTTAGGGTTTGCTTT | GAAGGTCGGAGTCAACGGATTCAGGTTAGGGTTAGGGTTTGCTTC | GTCTCCGCGGACTTTCCGGGATC | T | C |
| 715648889 | Chr01 | 43699409 | GAAGGTGACCAAGTTCATGCTCTCAGTGTTCGTCATGTGCCATGTA | GAAGGTCGGAGTCAACGGATTCTCAGTGTTCGTCATGTGCCATGTG | ATGGGACTGAGCAGAAACCTTA | A | G |
| 715645921 | Chr01 | 48941111 | GAAGGTGACCAAGTTCATGCTCACCAATAGTTTCAAACTACTAT | GAAGGTCGGAGTCAACGGATTCACCAATAGTTTCAAACTACTAC | TGATAACGTGATGTGATCTAGGTAAC | T | C |
| 715649479 | Chr02 | 6743 | GAAGGTGACCAAGTTCATGCTGGTTTAACTGGGGTGTCTAGA | GAAGGTCGGAGTCAACGGATTGGTTTAACTGGGGTGTCTAGG | TGTCAATCTCCAATCCACCAAG | A | G |
| 715640819 | Chr02 | 5855171 | GAAGGTGACCAAGTTCATGCTGGTGGCGAATGAATTCAAATTGC | GAAGGTCGGAGTCAACGGATTGGTGGCGAATGAATTCAAATTGT | AAAGCTATACATGCCACTAAAGTTTTG | C | T |
| 715647790 | Chr02 | 8927833 | GAAGGTGACCAAGTTCATGCTGGTTCATGCTTTAGGCTTAG | GAAGGTCGGAGTCAACGGATTGGTTCATGCTTTAGGCTTAA | ACCACTAGTAAAATTCAATTTCAGTTTCTT | G | A |
| 715642024 | Chr02 | 12142956 | GAAGGTGACCAAGTTCATGCTGGCTTCGTTTGCATGAATGA | GAAGGTCGGAGTCAACGGATTGGCTTCGTTTGCATGAATGG | CACCTTAGGACCAAGTAAACACAAACACAA | A | G |
| 715641350 | Chr02 | 21494397 | GAAGGTGACCAAGTTCATGCTGACTTGGACATTATTCTTATGGATG | GAAGGTCGGAGTCAACGGATTGACTTGGACATTATTCTTATGGATA | ATGTTAACTTTTTAAAAAAAAACCTC | G | A |
| 715648528 | Chr02 | 24042084 | GAAGGTGACCAAGTTCATGCTAACTAAAGGGCCTCGACCCC | GAAGGTCGGAGTCAACGGATTAACTAAAGGGCCTCGACCCT | GTTTTCATGGCACCGAGGCGAATACGAAGA | C | T |
| 715647099 | Chr02 | 32859750 | GAAGGTGACCAAGTTCATGCTGGTAAGTTTGGTGCTATTGCTT | GAAGGTCGGAGTCAACGGATTGGTAAGTTTGGTGCTATTGCTC | TAACCTTTTTCCTCACGTTTCTT | T | C |
| 715646488 | Chr02 | 36676613 | GAAGGTGACCAAGTTCATGCTCCATCCTTAAGAACTTGTGCAT | GAAGGTCGGAGTCAACGGATTCCATCCTTAAGAACTTGTGCAC | ACACCTTCAATGCTGTGGTAACA | T | C |
| 715647680 | Chr02 | 43900872 | GAAGGTGACCAAGTTCATGCTCAAGCTCTGTCATGCTCATGC | GAAGGTCGGAGTCAACGGATTCAAGCTCTGTCATGCTCATGT | ATAGTCTAGACCACATCACACG | C | T |
| 715648447 | Chr02 | 48531519 | GAAGGTGACCAAGTTCATGCTGGTTGTCAAAGGTTTAAAGCTG | GAAGGTCGGAGTCAACGGATTGGTTGTCAAAGGTTTAAAGCTA | GATTCTTCAGTAAGAGTAACCATG | G | A |
| ID | 染色体 Chromosome | 基因组位置 SNP position in chromosome v1.0 | FAM引物 Primer_AlleleFAM | HEX引物 Primer_AlleleHEX | 通用引物 Universal primer | FAM等 位变异 Allele_ FAM | HEX等 位变异 Allele_ HEX |
| 715644947 | Chr03 | 6366468 | GAAGGTGACCAAGTTCATGCTCCGTGTCACTGAGATCACTATC | GAAGGTCGGAGTCAACGGATTCCGTGTCACTGAGATCACTATT | AATACGAGGATGAATACATTTC | C | T |
| 715647580 | Chr03 | 12483803 | GAAGGTGACCAAGTTCATGCTAAAAACCTATCAAAGTAG | GAAGGTCGGAGTCAACGGATTAAAAACCTATCAAAGTAA | CTTTGTTCTTTGTTTCTCACAT | G | A |
| 715642031 | Chr03 | 15073563 | GAAGGTGACCAAGTTCATGCTTAGTTTTCATATTCAACC | GAAGGTCGGAGTCAACGGATTTAGTTTTCATATTCAACT | ATGAGAAATCAACTGGATCAAA | C | T |
| 715643408 | Chr03 | 27104732 | GAAGGTGACCAAGTTCATGCTGTCAGACAAACTCATTTACCCCG | GAAGGTCGGAGTCAACGGATTGTCAGACAAACTCATTTACCCCA | ATAAATCATTCCACCATCTAGA | G | A |
| 715649919 | Chr03 | 30251280 | GAAGGTGACCAAGTTCATGCTGCATTAATAGAGGATAGATGAAAC | GAAGGTCGGAGTCAACGGATTGCATTAATAGAGGATAGATGAAAT | GTTGTCTCATTGATAACCCTCA | C | T |
| 715650183 | Chr03 | 39786455 | GAAGGTGACCAAGTTCATGCTGTTTGCCCTTCTATTGGTATA | GAAGGTCGGAGTCAACGGATTGTTTGCCCTTCTATTGGTATC | TCTCCAACAAGCAAGAGCACTA | A | C |
| 715649769 | Chr04 | 3592 | GAAGGTGACCAAGTTCATGCTGTGCAAACCTGCAACTCAAAATTGC | GAAGGTCGGAGTCAACGGATTGTGCAAACCTGCAACTCAAAATTGA | ATCAGAACTGGAAGGATATCTT | C | A |
| 715647290 | Chr04 | 3109241 | GAAGGTGACCAAGTTCATGCTCCCTCTCTACCTAAGAACATGA | GAAGGTCGGAGTCAACGGATTCCCTCTCTACCTAAGAACATGC | GTTTTATGGTTAAGTGGTTAGTTTTGTAAG | A | C |
| 715641595 | Chr04 | 6493282 | GAAGGTGACCAAGTTCATGCTCGATATATCACCCATTAAC | GAAGGTCGGAGTCAACGGATTCGATATATCACCCATTAAA | ACCTTATAGTGTTTTGTTATCA | C | A |
| 715642949 | Chr04 | 15465426 | GAAGGTGACCAAGTTCATGCTGACTTCCTTATGCAGATCAACAT | GAAGGTCGGAGTCAACGGATTGACTTCCTTATGCAGATCAACAC | GAGTTTGATGAAGTACTTCATAAC | T | C |
| 715650920 | Chr04 | 21774557 | GAAGGTGACCAAGTTCATGCTTCCCACACACACTCACATCCAACAC | GAAGGTCGGAGTCAACGGATTTCCCACACACACTCACATCCAACAT | TGCTTAAAAGGACCACCTCGCTTA | C | T |
| 715650671 | Chr04 | 25684069 | GAAGGTGACCAAGTTCATGCTTGTTAGAACGCTGGTGCAT | GAAGGTCGGAGTCAACGGATTTGTTAGAACGCTGGTGCAG | GAAAAGTAAAATCTGCATCACC | T | G |
| 715642248 | Chr04 | 34571194 | GAAGGTGACCAAGTTCATGCTTCCCTTAACTTACTTGTGG | GAAGGTCGGAGTCAACGGATTTCCCTTAACTTACTTGTGA | CTTTTCAGGCTTGCTTTGCAGAC | G | A |
| 715639507 | Chr04 | 37382175 | GAAGGTGACCAAGTTCATGCTGTCACGGTGAATATTACCCG | GAAGGTCGGAGTCAACGGATTGTCACGGTGAATATTACCCA | AGAAGGTCAGATAGATAGGTCC | G | A |
| 715646125 | Chr04 | 43085220 | GAAGGTGACCAAGTTCATGCTCCTAATAAGCTTAAGTCAATACCTG | GAAGGTCGGAGTCAACGGATTCCTAATAAGCTTAAGTCAATACCTT | AAAACTGAAAAGTGAAATGCTC | G | T |
| 715639543 | Chr05 | 6447545 | GAAGGTGACCAAGTTCATGCTGTCGATTGAAGGCTTTATCTCAT | GAAGGTCGGAGTCAACGGATTGTCGATTGAAGGCTTTATCTCAC | ACATTGAAATCAAACTCACACACTAA | T | C |
| 715644469 | Chr05 | 9344921 | GAAGGTGACCAAGTTCATGCTGAGTGTGCCCTAAAACAAAATATC | GAAGGTCGGAGTCAACGGATTGAGTGTGCCCTAAAACAAAATATA | CTTGTAGCAGTTTTCTATCACGAGAGTATC | C | A |
| 715645339 | Chr05 | 39192763 | GAAGGTGACCAAGTTCATGCTGCTGAGCTAAGACTATGAATCGGGC | GAAGGTCGGAGTCAACGGATTGCTGAGCTAAGACTATGAATCGGGT | TCCTTGTTAAATGTGCTCATTC | C | T |
| 715646737 | Chr05 | 40469914 | GAAGGTGACCAAGTTCATGCTACACACCCTTCTTTCTCTCCTCTCG | GAAGGTCGGAGTCAACGGATTACACACCCTTCTTTCTCTCCTCTCT | AGGACAACCACTCATTTTTGCTC | G | T |
| 715650987 | Chr06 | 15998 | GAAGGTGACCAAGTTCATGCTCCAAAGGAGTCCACATTCA | GAAGGTCGGAGTCAACGGATTCCAAAGGAGTCCACATTCC | GGCCTACTAGTTTTACAAGAATGTTC | A | C |
| 715647844 | Chr06 | 6504361 | GAAGGTGACCAAGTTCATGCTCTCTTCTAATTCAGGACCAAATC | GAAGGTCGGAGTCAACGGATTCTCTTCTAATTCAGGACCAAATT | CGAGGTAGGCAAGCCTTCCCTT | C | T |
| 715640066 | Chr06 | 9341450 | GAAGGTGACCAAGTTCATGCTGGGGCCAACCTCAAATTTA | GAAGGTCGGAGTCAACGGATTGGGGCCAACCTCAAATTTG | GTGTCCCCTTAATCATTTCACATTGTTC | A | G |
| 715645954 | Chr06 | 15786595 | GAAGGTGACCAAGTTCATGCTGACCTTCTGAAGGAACTGA | GAAGGTCGGAGTCAACGGATTGACCTTCTGAAGGAACTGG | ATGCAGGTAAAACCAGTGGTCCAGAC | A | G |
| 715650222 | Chr06 | 18501260 | GAAGGTGACCAAGTTCATGCTGTCACAATGAGGCCTTCTCTC | GAAGGTCGGAGTCAACGGATTGTCACAATGAGGCCTTCTCTT | ATCAGTATGATTCTACATGTTTTTGG | C | T |
| 715646818 | Chr06 | 21496816 | GAAGGTGACCAAGTTCATGCTAAATTACACTAGCATAAT | GAAGGTCGGAGTCAACGGATTAAATTACACTAGCATAAC | GAGCCATTTGTGTTTGAATATG | T | C |
| 715645677 | Chr06 | 27370471 | GAAGGTGACCAAGTTCATGCTGAATAAAGAGTGATGATA | GAAGGTCGGAGTCAACGGATTGAATAAAGAGTGATGATG | ACATGGCCATGTATTCCGTCTTGGTTTGGT | A | G |
| 715639203 | Chr06 | 30199530 | GAAGGTGACCAAGTTCATGCTGACATGTTCTTGCATTATGA | GAAGGTCGGAGTCAACGGATTGACATGTTCTTGCATTATGG | TGGTACCTTTGATGTATCACACT | A | G |
| 715639367 | Chr06 | 31688381 | GAAGGTGACCAAGTTCATGCTTCCTCTTCCTGCACCATAGTC | GAAGGTCGGAGTCAACGGATTTCCTCTTCCTGCACCATAGTT | CATGTGAGCAAGTGTTTGGTGGCATTGAAC | C | T |
| ID | 染色体 Chromosome | 基因组位置 SNP position in chromosome v1.0 | FAM引物 Primer_AlleleFAM | HEX引物 Primer_AlleleHEX | 通用引物 Universal primer | FAM等 位变异 Allele_ FAM | HEX等 位变异 Allele_ HEX |
| 715648393 | Chr07 | 61520 | GAAGGTGACCAAGTTCATGCTGTTGTCTTTCAATGAACGAT | GAAGGTCGGAGTCAACGGATTGTTGTCTTTCAATGAACGAC | AAGGCAGGAATCTTTAAAAGCT | T | C |
| 715645699 | Chr07 | 1153000 | GAAGGTGACCAAGTTCATGCTTGAGTTCACGTGCATTGGGCATG | GAAGGTCGGAGTCAACGGATTTGAGTTCACGTGCATTGGGCATT | CCAAACAACATAAAGCAATTTCTAACAAAC | G | T |
| 715648625 | Chr07 | 9516304 | GAAGGTGACCAAGTTCATGCTGCATGTAGTGTTTTTAGTTTC | GAAGGTCGGAGTCAACGGATTGCATGTAGTGTTTTTAGTTTT | CCATATCACTAGGACTCTGAAC | C | T |
| 715648880 | Chr07 | 12261154 | GAAGGTGACCAAGTTCATGCTGACATCATCTTTGCAGTGAAC | GAAGGTCGGAGTCAACGGATTGACATCATCTTTGCAGTGAAT | TAATGGTCTTGATAAGGAGAGC | C | T |
| 715650406 | Chr07 | 15316545 | GAAGGTGACCAAGTTCATGCTTGACATAAATTGTGAAAG | GAAGGTCGGAGTCAACGGATTTGACATAAATTGTGAAAA | AAAGAATGTGAAAGAAGTCCAC | G | A |
| 715642111 | Chr07 | 18479982 | GAAGGTGACCAAGTTCATGCTCCGTAAGCTGGGACTTAACAGAAAT | GAAGGTCGGAGTCAACGGATTCCGTAAGCTGGGACTTAACAGAAAG | CCTACATTTAGGGTCCTTGTGTTTTT | T | G |
| 715643102 | Chr07 | 24160043 | GAAGGTGACCAAGTTCATGCTAGCAAGGGCGGATCAAAGGA | GAAGGTCGGAGTCAACGGATTAGCAAGGGCGGATCAAAGGC | CCTCCCCTTGAGAATCCTCCTG | A | C |
| 715640138 | Chr07 | 30148305 | GAAGGTGACCAAGTTCATGCTCTGTTTGCTTCTGTTTACTGC | GAAGGTCGGAGTCAACGGATTCTGTTTGCTTCTGTTTACTGT | TCTCTTCCAGTTCTTCCGTGCTAAA | C | T |
| 715641587 | Chr07 | 33842222 | GAAGGTGACCAAGTTCATGCTGACCCACTTAATCCATCTAC | GAAGGTCGGAGTCAACGGATTGACCCACTTAATCCATCTAT | AGGACTCAGTAAGGTGGGGCTTTCTTCTTC | C | T |
| 715639238 | Chr07 | 42558166 | GAAGGTGACCAAGTTCATGCTATTGGATCAGGTGTGATGCCG | GAAGGTCGGAGTCAACGGATTATTGGATCAGGTGTGATGCCA | AGGGGTGATCATGACACGCTTAC | G | A |
| 715646608 | Chr07 | 45792744 | GAAGGTGACCAAGTTCATGCTGCTGATTCATCATGCTCAC | GAAGGTCGGAGTCAACGGATTGCTGATTCATCATGCTCAA | GAATCTAAACTTGAACCACGTGT | C | A |
| 715646685 | Chr08 | 129003 | GAAGGTGACCAAGTTCATGCTGGATTTGGGGGAGAAGAAG | GAAGGTCGGAGTCAACGGATTGGATTTGGGGGAGAAGAAA | AAATTTTTTTCTCACCCCCACA | G | A |
| 715645832 | Chr08 | 3020556 | GAAGGTGACCAAGTTCATGCTCTCATGTAAACAATTGAT | GAAGGTCGGAGTCAACGGATTCTCATGTAAACAATTGAC | TTGCACATGTAATGCTTGGAAG | T | C |
| 715649757 | Chr08 | 6483262 | GAAGGTGACCAAGTTCATGCTCAAGCCTAATGACCACTGACACACA | GAAGGTCGGAGTCAACGGATTCAAGCCTAATGACCACTGACACACC | TGAAGTATTTGCAGAAAATTGGAGAC | A | C |
| 715647414 | Chr08 | 9422765 | GAAGGTGACCAAGTTCATGCTCAACTGCGACAAGATGGTACT | GAAGGTCGGAGTCAACGGATTCAACTGCGACAAGATGGTACC | ATAAACAAAAAATAGTGTCTCG | T | C |
| 715648702 | Chr08 | 15144723 | GAAGGTGACCAAGTTCATGCTGTCTTCTTCTTCCTTTTCTATT | GAAGGTCGGAGTCAACGGATTGTCTTCTTCTTCCTTTTCTATC | TTGCATAAAACGCATTAAATGT | T | C |
| 715644789 | Chr08 | 24424087 | GAAGGTGACCAAGTTCATGCTGCCTTAGTTGACACCTCCATTT | GAAGGTCGGAGTCAACGGATTGCCTTAGTTGACACCTCCATTC | CTCTTCATCGTGTGATGGTTGG | T | C |
| 715639796 | Chr08 | 33391365 | GAAGGTGACCAAGTTCATGCTGAGGAAAGGAATGCTTTCAACTG | GAAGGTCGGAGTCAACGGATTGAGGAAAGGAATGCTTTCAACTA | GGTGGTCAAATTCAACTAGGATGGC | G | A |
| 715640428 | Chr08 | 36879821 | GAAGGTGACCAAGTTCATGCTGCTACAAACAAATCATGATGCATCC | GAAGGTCGGAGTCAACGGATTGCTACAAACAAATCATGATGCATCT | ACTCTGTCATGAACGCGGTCTT | C | T |
| 715650893 | Chr08 | 39711359 | GAAGGTGACCAAGTTCATGCTTCATATCTTCAAAAAACT | GAAGGTCGGAGTCAACGGATTTCATATCTTCAAAAAACC | AGTCACATCCCATCAAATTCGG | T | C |
| 715648689 | Chr08 | 48679942 | GAAGGTGACCAAGTTCATGCTTATTTGCATGGCCCGACCAACCTC | GAAGGTCGGAGTCAACGGATTTATTTGCATGGCCCGACCAACCTT | CTTTAGTTTAGTAGGTTAAGTAAT | C | T |
| 715639302 | Chr08 | 51313104 | GAAGGTGACCAAGTTCATGCTTCTCTAACTCGTGCGTACG | GAAGGTCGGAGTCAACGGATTTCTCTAACTCGTGCGTACA | GTTTGTTGGGTTTATTATCGGA | G | A |
| 715650660 | Chr08 | 59634577 | GAAGGTGACCAAGTTCATGCTACATGATAAACACAAAAA | GAAGGTCGGAGTCAACGGATTACATGATAAACACAAAAG | TAGCCATATTGTTCTTCTATAAAAAAGCAG | A | G |
| 715650429 | Chr09 | 298696 | GAAGGTGACCAAGTTCATGCTCATTTGGTACCTCGTCTCAG | GAAGGTCGGAGTCAACGGATTCATTTGGTACCTCGTCTCAA | ACAAGAACTGGAACTAGTATAC | G | A |
| 715639476 | Chr09 | 3133765 | GAAGGTGACCAAGTTCATGCTCCTCTTACTCAAGAAAGCCAAGAT | GAAGGTCGGAGTCAACGGATTCCTCTTACTCAAGAAAGCCAAGAC | TCTATCTATCACGGATAAGTTG | T | C |
| 715639339 | Chr09 | 12175377 | GAAGGTGACCAAGTTCATGCTGCTTATAGTGTGGAGAGCAG | GAAGGTCGGAGTCAACGGATTGCTTATAGTGTGGAGAGCAA | GATTTCTCAATAGAAACATGCC | G | A |
| 715646043 | Chr09 | 21807115 | GAAGGTGACCAAGTTCATGCTGTGAGTACTAAGATTTGTGGTCTGG | GAAGGTCGGAGTCAACGGATTGTGAGTACTAAGATTTGTGGTCTGA | CGATAAGAACATTTGACACCCACTGT | G | A |
| 715646065 | Chr09 | 24807436 | GAAGGTGACCAAGTTCATGCTCCCTTGTGAGAAAGTATTGATTGTG | GAAGGTCGGAGTCAACGGATTCCCTTGTGAGAAAGTATTGATTGTA | GCCAATTTAGAAAGTTCTCCATGT | G | A |
| ID | 染色体 Chromosome | 基因组位置 SNP position in chromosome v1.0 | FAM引物 Primer_AlleleFAM | HEX引物 Primer_AlleleHEX | 通用引物 Universal primer | FAM等 位变异 Allele_ FAM | HEX等 位变异 Allele_ HEX |
| 715643007 | Chr10 | 95419 | GAAGGTGACCAAGTTCATGCTAACAATACTACGTTTAAT | GAAGGTCGGAGTCAACGGATTAACAATACTACGTTTAAC | GTGGACTGAGCCCTGTTAACTGGAAGCTAA | T | C |
| 715650121 | Chr10 | 6367592 | GAAGGTGACCAAGTTCATGCTTATTAAGGGAAAGATCGAC | GAAGGTCGGAGTCAACGGATTTATTAAGGGAAAGATCGAT | TGGACCCATGACCACAAGTTAAGAGCCTTC | C | T |
| 715641901 | Chr10 | 18965279 | GAAGGTGACCAAGTTCATGCTCCCCTAGTAGCCTTATACCAAC | GAAGGTCGGAGTCAACGGATTCCCCTAGTAGCCTTATACCAAT | TATTGGTAAGCCTGTTGAGGCA | C | T |
| 715644023 | Chr10 | 27898392 | GAAGGTGACCAAGTTCATGCTGAGCATCGTTTGGTAGAGCACAAGT | GAAGGTCGGAGTCAACGGATTGAGCATCGTTTGGTAGAGCACAAGG | GGCGCCCACCGTGGGGTTCGAA | T | G |
| 715640671 | Chr10 | 30260059 | GAAGGTGACCAAGTTCATGCTAGAGATTGGATGCATGAGTCGCCA | GAAGGTCGGAGTCAACGGATTAGAGATTGGATGCATGAGTCGCCG | GTGCGTAAGGCTGCCCTACCGAGG | A | G |
| 715639617 | Chr10 | 33411083 | GAAGGTGACCAAGTTCATGCTGCCTCATGCTTTATTGACAGGGC | GAAGGTCGGAGTCAACGGATTGCCTCATGCTTTATTGACAGGGT | ACACTTGTTTCTTTGCATCATGGA | C | T |
| 715640169 | Chr10 | 36497500 | GAAGGTGACCAAGTTCATGCTCACCTTGCCAGAATACTTCACCT | GAAGGTCGGAGTCAACGGATTCACCTTGCCAGAATACTTCACCC | CTGAGGATCCTGGGAAGCATTG | T | C |
| 715639540 | Chr10 | 42706527 | GAAGGTGACCAAGTTCATGCTGCTCACATCTTACTCATACCAACTG | GAAGGTCGGAGTCAACGGATTGCTCACATCTTACTCATACCAACTA | AACAATGGATGTGGTGACTTATCA | G | A |
| 715641318 | Chr11 | 16520 | GAAGGTGACCAAGTTCATGCTAAATACTTTCAACGTTTG | GAAGGTCGGAGTCAACGGATTAAATACTTTCAACGTTTA | AACCCTCTCGTGTGAAATTTCCACTATGAC | G | A |
| 715645562 | Chr11 | 3190094 | GAAGGTGACCAAGTTCATGCTTTTGATGAATGAATTTCG | GAAGGTCGGAGTCAACGGATTTTTGATGAATGAATTTCA | GTTTATATTGGAGCCTGCATGTGTTAGCTA | G | A |
| 715650149 | Chr11 | 6641086 | GAAGGTGACCAAGTTCATGCTGACCCAGCAGCTATGTCTGTTTTA | GAAGGTCGGAGTCAACGGATTGACCCAGCAGCTATGTCTGTTTTG | AGAATGCATAGCACTTATTTGATTGTG | A | G |
| 715639655 | Chr11 | 12487977 | GAAGGTGACCAAGTTCATGCTTCTCACCACCTGTACTCCAAACCTA | GAAGGTCGGAGTCAACGGATTTCTCACCACCTGTACTCCAAACCTG | TCGACATTAAACAATACCAAGGAGAATCC | A | G |
| 715644528 | Chr11 | 21570007 | GAAGGTGACCAAGTTCATGCTCCAGCTACACCTCAATAGCTAGA | GAAGGTCGGAGTCAACGGATTCCAGCTACACCTCAATAGCTAGG | GAAGGAGGCTGGCCAAAGTACCCACA | A | G |
| 715644319 | Chr11 | 24580005 | GAAGGTGACCAAGTTCATGCTGGAAGGTACAAGGGACGAGTATTTG | GAAGGTCGGAGTCAACGGATTGGAAGGTACAAGGGACGAGTATTTA | AACGCCCGAGGACGAGACGGTACACTAA | G | A |
| 715641793 | Chr11 | 33339240 | GAAGGTGACCAAGTTCATGCTTTTGAAGTGGGGGTTTGGAGTCCG | GAAGGTCGGAGTCAACGGATTTTTGAAGTGGGGGTTTGGAGTCCA | GCCAATAAGAAACATCCCGACC | G | A |
| 715648007 | Chr11 | 42300383 | GAAGGTGACCAAGTTCATGCTCAAACAATAGGAGAATAGCATT | GAAGGTCGGAGTCAACGGATTCAAACAATAGGAGAATAGCATC | TGAACCTTTTAACACCTCCTTA | T | C |
表4 不同染色体上有多态标记的分布情况
Table 4 Distribution of the polymorphic markers on different chromosomes
| 染色体 Chromosome | 标记数量 Number of marker |
|---|---|
| Chr01 | 6 |
| Chr02 | 10 |
| Chr03 | 6 |
| Chr04 | 9 |
| Chr05 | 4 |
| Chr06 | 9 |
| Chr07 | 11 |
| Chr08 | 12 |
| Chr09 | 5 |
| Chr10 | 8 |
| Chr11 | 8 |
表4 不同染色体上有多态标记的分布情况
Table 4 Distribution of the polymorphic markers on different chromosomes
| 染色体 Chromosome | 标记数量 Number of marker |
|---|---|
| Chr01 | 6 |
| Chr02 | 10 |
| Chr03 | 6 |
| Chr04 | 9 |
| Chr05 | 4 |
| Chr06 | 9 |
| Chr07 | 11 |
| Chr08 | 12 |
| Chr09 | 5 |
| Chr10 | 8 |
| Chr11 | 8 |
表5 八十八个KASP标记在44份种质中扩增结果
Table 5 Amplification result of 88 KASP markers in 44 accessions
| ID | 等位变异 Alleles | 缺失率 Missing rate | 多态性信息含量 Polymorphism information content | 最小等位 变异频率 Minor allele frequency |
|---|---|---|---|---|
| 715639203 | A/G | 0.05 | 0.171 | 0.045 |
| 715639238 | G/A | 0 | 0.491 | 0.432 |
| 715639302 | G/A | 0.05 | 0.171 | 0.045 |
| 715639339 | G/A | 0 | 0.087 | 0.045 |
| 715639367 | C/T | 0.20 | 0.435 | 0.045 |
| 715639381 | T/C | 0 | 0.087 | 0.045 |
| 715639476 | T/C | 0.05 | 0.171 | 0.045 |
| 715639507 | G/A | 0 | 0.201 | 0.114 |
| 715639540 | G/A | 0 | 0.087 | 0.045 |
| 715639543 | T/C | 0.02 | 0.130 | 0.045 |
| 715639617 | C/T | 0 | 0.044 | 0.023 |
| 715639655 | A/G | 0 | 0.087 | 0.045 |
| 715639796 | G/A | 0 | 0.201 | 0.114 |
| 715640066 | A/G | 0 | 0.325 | 0.205 |
| 715640138 | C/T | 0 | 0.165 | 0.091 |
| 715640169 | T/C | 0 | 0.127 | 0.068 |
| 715640428 | C/T | 0 | 0.087 | 0.045 |
| 715640479 | G/T | 0.09 | 0.252 | 0.045 |
| 715640671 | A/G | 0 | 0.087 | 0.045 |
| 715640819 | C/T | 0 | 0.087 | 0.045 |
| 715641318 | G/A | 0.02 | 0.130 | 0.045 |
| 715641350 | G/A | 0.11 | 0.576 | 0.318 |
| 715641587 | C/T | 0 | 0.165 | 0.091 |
| 715641595 | C/A | 0.27 | 0.726 | 0.432 |
| 715641793 | G/A | 0 | 0.087 | 0.045 |
| 715641901 | C/T | 0.20 | 0.466 | 0.068 |
| 715642024 | A/G | 0.11 | 0.291 | 0.045 |
| 715642031 | C/T | 0.20 | 0.466 | 0.068 |
| 715642111 | T/G | 0 | 0.165 | 0.091 |
| 715642248 | G/A | 0.02 | 0.130 | 0.045 |
| 715642949 | T/C | 0 | 0.165 | 0.091 |
| 715643007 | T/C | 0.05 | 0.543 | 0.455 |
| 715643102 | A/C | 0 | 0.087 | 0.045 |
| 715643138 | C/A | 0.02 | 0.130 | 0.045 |
| 715643408 | G/A | 0.05 | 0.171 | 0.045 |
| 715644023 | T/G | 0.05 | 0.210 | 0.068 |
| 715644319 | G/A | 0 | 0.087 | 0.045 |
| 715644469 | C/A | 0 | 0.087 | 0.045 |
| 715644528 | A/G | 0 | 0.087 | 0.045 |
| 715644789 | T/C | 0 | 0.087 | 0.045 |
| 715644947 | C/T | 0 | 0.087 | 0.045 |
| 715645089 | G/T | 0.18 | 0.628 | 0.273 |
| 715645339 | C/T | 0 | 0.087 | 0.045 |
| 715645562 | G/A | 0.05 | 0.212 | 0.045 |
| 715645677 | A/G | 0 | 0.171 | 0.045 |
| 715645699 | G/T | 0.02 | 0.169 | 0.068 |
| 715645832 | T/C | 0.09 | 0.536 | 0.295 |
| ID | 等位变异 Alleles | 缺失率 Missing rate | 多态性信息含量 Polymorphism information content | 最小等位 变异频率 Minor allele frequency |
| 715645921 | T/C | 0 | 0.127 | 0.068 |
| 715645954 | A/G | 0.07 | 0.212 | 0.045 |
| 715646043 | G/A | 0.23 | 0.469 | 0.045 |
| 715646065 | G/A | 0.07 | 0.349 | 0.136 |
| 715646125 | G/T | 0 | 0.397 | 0.273 |
| 715646488 | T/C | 0 | 0.236 | 0.136 |
| 715646608 | C/A | 0 | 0.127 | 0.068 |
| 715646685 | G/A | 0.02 | 0.510 | 0.409 |
| 715646737 | G/T | 0.02 | 0.130 | 0.045 |
| 715646818 | T/C | 0.09 | 0.601 | 0.386 |
| 715647099 | T/C | 0 | 0.491 | 0.432 |
| 715647290 | A/C | 0 | 0.351 | 0.227 |
| 715647414 | T/C | 0 | 0.268 | 0.159 |
| 715647580 | G/A | 0.07 | 0.404 | 0.182 |
| 715647680 | C/T | 0 | 0.044 | 0.023 |
| 715647790 | G/A | 0.02 | 0.274 | 0.136 |
| 715647844 | C/T | 0 | 0.165 | 0.091 |
| 715648007 | T/C | 0.02 | 0.169 | 0.068 |
| 715648393 | T/C | 0.05 | 0.507 | 0.341 |
| 715648447 | G/A | 0 | 0.087 | 0.045 |
| 715648528 | C/T | 0 | 0.397 | 0.273 |
| 715648625 | C/T | 0 | 0.268 | 0.159 |
| 715648689 | C/T | 0.30 | 0.590 | 0.068 |
| 715648702 | T/C | 0.25 | 0.557 | 0.091 |
| 715648880 | C/T | 0 | 0.165 | 0.091 |
| 715648889 | A/G | 0 | 0.087 | 0.045 |
| 715649479 | A/G | 0.02 | 0.130 | 0.045 |
| 715649757 | A/C | 0.05 | 0.280 | 0.114 |
| 715649769 | C/A | 0 | 0.044 | 0.023 |
| 715649919 | C/T | 0 | 0.449 | 0.341 |
| 715650121 | C/T | 0 | 0.268 | 0.159 |
| 715650149 | A/G | 0 | 0.236 | 0.136 |
| 715650183 | A/C | 0.02 | 0.130 | 0.045 |
| 715650222 | C/T | 0.05 | 0.210 | 0.068 |
| 715650406 | G/A | 0.09 | 0.354 | 0.114 |
| 715650429 | G/A | 0 | 0.351 | 0.227 |
| 715650660 | A/G | 0.07 | 0.252 | 0.045 |
| 715650671 | T/G | 0.23 | 0.527 | 0.091 |
| 715650893 | T/C | 0.09 | 0.252 | 0.045 |
| 715650920 | C/T | 0.02 | 0.206 | 0.091 |
| 715650987 | A/C | 0.02 | 0.206 | 0.091 |
表5 八十八个KASP标记在44份种质中扩增结果
Table 5 Amplification result of 88 KASP markers in 44 accessions
| ID | 等位变异 Alleles | 缺失率 Missing rate | 多态性信息含量 Polymorphism information content | 最小等位 变异频率 Minor allele frequency |
|---|---|---|---|---|
| 715639203 | A/G | 0.05 | 0.171 | 0.045 |
| 715639238 | G/A | 0 | 0.491 | 0.432 |
| 715639302 | G/A | 0.05 | 0.171 | 0.045 |
| 715639339 | G/A | 0 | 0.087 | 0.045 |
| 715639367 | C/T | 0.20 | 0.435 | 0.045 |
| 715639381 | T/C | 0 | 0.087 | 0.045 |
| 715639476 | T/C | 0.05 | 0.171 | 0.045 |
| 715639507 | G/A | 0 | 0.201 | 0.114 |
| 715639540 | G/A | 0 | 0.087 | 0.045 |
| 715639543 | T/C | 0.02 | 0.130 | 0.045 |
| 715639617 | C/T | 0 | 0.044 | 0.023 |
| 715639655 | A/G | 0 | 0.087 | 0.045 |
| 715639796 | G/A | 0 | 0.201 | 0.114 |
| 715640066 | A/G | 0 | 0.325 | 0.205 |
| 715640138 | C/T | 0 | 0.165 | 0.091 |
| 715640169 | T/C | 0 | 0.127 | 0.068 |
| 715640428 | C/T | 0 | 0.087 | 0.045 |
| 715640479 | G/T | 0.09 | 0.252 | 0.045 |
| 715640671 | A/G | 0 | 0.087 | 0.045 |
| 715640819 | C/T | 0 | 0.087 | 0.045 |
| 715641318 | G/A | 0.02 | 0.130 | 0.045 |
| 715641350 | G/A | 0.11 | 0.576 | 0.318 |
| 715641587 | C/T | 0 | 0.165 | 0.091 |
| 715641595 | C/A | 0.27 | 0.726 | 0.432 |
| 715641793 | G/A | 0 | 0.087 | 0.045 |
| 715641901 | C/T | 0.20 | 0.466 | 0.068 |
| 715642024 | A/G | 0.11 | 0.291 | 0.045 |
| 715642031 | C/T | 0.20 | 0.466 | 0.068 |
| 715642111 | T/G | 0 | 0.165 | 0.091 |
| 715642248 | G/A | 0.02 | 0.130 | 0.045 |
| 715642949 | T/C | 0 | 0.165 | 0.091 |
| 715643007 | T/C | 0.05 | 0.543 | 0.455 |
| 715643102 | A/C | 0 | 0.087 | 0.045 |
| 715643138 | C/A | 0.02 | 0.130 | 0.045 |
| 715643408 | G/A | 0.05 | 0.171 | 0.045 |
| 715644023 | T/G | 0.05 | 0.210 | 0.068 |
| 715644319 | G/A | 0 | 0.087 | 0.045 |
| 715644469 | C/A | 0 | 0.087 | 0.045 |
| 715644528 | A/G | 0 | 0.087 | 0.045 |
| 715644789 | T/C | 0 | 0.087 | 0.045 |
| 715644947 | C/T | 0 | 0.087 | 0.045 |
| 715645089 | G/T | 0.18 | 0.628 | 0.273 |
| 715645339 | C/T | 0 | 0.087 | 0.045 |
| 715645562 | G/A | 0.05 | 0.212 | 0.045 |
| 715645677 | A/G | 0 | 0.171 | 0.045 |
| 715645699 | G/T | 0.02 | 0.169 | 0.068 |
| 715645832 | T/C | 0.09 | 0.536 | 0.295 |
| ID | 等位变异 Alleles | 缺失率 Missing rate | 多态性信息含量 Polymorphism information content | 最小等位 变异频率 Minor allele frequency |
| 715645921 | T/C | 0 | 0.127 | 0.068 |
| 715645954 | A/G | 0.07 | 0.212 | 0.045 |
| 715646043 | G/A | 0.23 | 0.469 | 0.045 |
| 715646065 | G/A | 0.07 | 0.349 | 0.136 |
| 715646125 | G/T | 0 | 0.397 | 0.273 |
| 715646488 | T/C | 0 | 0.236 | 0.136 |
| 715646608 | C/A | 0 | 0.127 | 0.068 |
| 715646685 | G/A | 0.02 | 0.510 | 0.409 |
| 715646737 | G/T | 0.02 | 0.130 | 0.045 |
| 715646818 | T/C | 0.09 | 0.601 | 0.386 |
| 715647099 | T/C | 0 | 0.491 | 0.432 |
| 715647290 | A/C | 0 | 0.351 | 0.227 |
| 715647414 | T/C | 0 | 0.268 | 0.159 |
| 715647580 | G/A | 0.07 | 0.404 | 0.182 |
| 715647680 | C/T | 0 | 0.044 | 0.023 |
| 715647790 | G/A | 0.02 | 0.274 | 0.136 |
| 715647844 | C/T | 0 | 0.165 | 0.091 |
| 715648007 | T/C | 0.02 | 0.169 | 0.068 |
| 715648393 | T/C | 0.05 | 0.507 | 0.341 |
| 715648447 | G/A | 0 | 0.087 | 0.045 |
| 715648528 | C/T | 0 | 0.397 | 0.273 |
| 715648625 | C/T | 0 | 0.268 | 0.159 |
| 715648689 | C/T | 0.30 | 0.590 | 0.068 |
| 715648702 | T/C | 0.25 | 0.557 | 0.091 |
| 715648880 | C/T | 0 | 0.165 | 0.091 |
| 715648889 | A/G | 0 | 0.087 | 0.045 |
| 715649479 | A/G | 0.02 | 0.130 | 0.045 |
| 715649757 | A/C | 0.05 | 0.280 | 0.114 |
| 715649769 | C/A | 0 | 0.044 | 0.023 |
| 715649919 | C/T | 0 | 0.449 | 0.341 |
| 715650121 | C/T | 0 | 0.268 | 0.159 |
| 715650149 | A/G | 0 | 0.236 | 0.136 |
| 715650183 | A/C | 0.02 | 0.130 | 0.045 |
| 715650222 | C/T | 0.05 | 0.210 | 0.068 |
| 715650406 | G/A | 0.09 | 0.354 | 0.114 |
| 715650429 | G/A | 0 | 0.351 | 0.227 |
| 715650660 | A/G | 0.07 | 0.252 | 0.045 |
| 715650671 | T/G | 0.23 | 0.527 | 0.091 |
| 715650893 | T/C | 0.09 | 0.252 | 0.045 |
| 715650920 | C/T | 0.02 | 0.206 | 0.091 |
| 715650987 | A/C | 0.02 | 0.206 | 0.091 |
图5 四十四份菜豆种质的PCA分析结果
Fig.5 PCA analysis result in the 44 common bean accessions
图5 四十四份菜豆种质的PCA分析结果
Fig.5 PCA analysis result in the 44 common bean accessions
图6 四十四份菜豆种质的系统进化分析
Fig.6 Phylogenetic tree analysis in the 44 common bean accessions
图6 四十四份菜豆种质的系统进化分析
Fig.6 Phylogenetic tree analysis in the 44 common bean accessions
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