日粮钙磷水平对马岗鹅生长性能及肝脏基因表达的影响

doi:10.3969/j.issn.1004-1524.20221657

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (11): 2533-2542.DOI: 10.3969/j.issn.1004-1524.20221657

日粮钙磷水平对马岗鹅生长性能及肝脏基因表达的影响

李虹仪(), 周润盛, 梁笑玲, 张楚玥, 吕祺欣, 杨长华, 张茂^*()

韶关学院英东生物与农业学院,广东韶关 512000

收稿日期:2022-11-19 出版日期:2023-11-25 发布日期:2023-12-04
作者简介:李虹仪(1984—),女,广东汕头人,博士,研究方向为动物营养生理与生物化学。E-mail:politician_137@163.com
通讯作者: * 张茂,E-mail:zm18email@163.com
基金资助:
广东省大学生创新创业训练计划(S202110576068);广东省自然科学基金面上项目(2021A1515010483)

Effect of dietary calcium and phosphorus level on Magang geese growth performance and liver gene expression

LI Hongyi(), ZHOU Runsheng, LIANG Xiaoling, ZHANG Chuyue, LYU Qixin, YANG Changhua, ZHANG Mao^*()

Henry Fok College of Biology and Agriculture, Shaoguan University, Shaoguan 512000, Guangdong, China

Received:2022-11-19 Online:2023-11-25 Published:2023-12-04

摘要/Abstract

摘要：

为了研究日粮中钙磷水平对马岗鹅生长性能及肝脏代谢相关基因的影响。试验选用48只体重相近、公母各半的50日龄马岗鹅,随机分为3个组,每组2个重复,每个重复8只,每笼饲养2只。3个组在相同日粮(含钙0.16%、有效磷0.11%)的基础上,分别添加0%、0.15%和0.3%的磷酸氢钙。预饲5 d,试验期28 d,期间记录日采食量,定期采集粪样及血样,测定样品中的钙磷含量。试验结束每组随机抽取8只马岗鹅进行屠宰及肝脏组织样采集,测定其屠宰性能和磷酸化酶活性,转录组测序及荧光定量PCR检测肝脏基因的表达变化。结果表明:对照组试验鹅的采食量高于其他两组(P<0.05),平均日增重、屠宰性能均高于0.15%磷酸氢钙组(P<0.05),而与0.3%磷酸氢钙组无显著差异;3组鹅血钙血磷含量无显著性差异(P>0.05),而粪钙含量随钙磷水平的提高而增加(P<0.05)。转录组测序结果显示,对照组与0.15%磷酸氢钙组之间差异较少,而与0.3%磷酸氢钙组差异较明显,经过分析,差异显著基因较多富集在糖代谢通路,荧光定量PCR结果亦与转录组测序一致,糖代谢相关酶磷酸化酶的活性则随钙磷水平的提高而减少(P<0.05)。日粮中钙磷水平会影响马岗鹅的采食量,同时影响肝脏的糖脂代谢。

关键词: 马岗鹅, 钙磷水平, 生长性能, 基因表达, 代谢

Abstract:

To study the effect of calcium and phosphorus levels in diet on growth performance and liver metabolism related genes in Magang geese, a total of forty eight Magang geese of 50-day-old were randomly divided into three groups, with male and female in half. Each group had two replicates with eight geese per replicate, every two geese were kept in one cage. On the basis of the same diet, 0%, 0.15% and 0.3% calcium hydrogen phosphate were added respectively, each group was pre-fed for five days and fed for twenty-eight days, during which the daily feed intake was recorded, fecal samples and blood samples were collected regularly, and the content of calcium and phosphorus in geese samples were determined. At the end of the experiment, eight Magang geese were randomly selected from each group for slaughter and the slaughter performance and phosphorylase activity were measured. The liver tissue of each geese were collected for transcriptome sequencing, fluorescence quantitative PCR and enzyme activity detection. The results showed that the feed intake of control group was significantly higher than that of the other two groups (P<0.05). The average daily gain and carcass traits were significantly higher than that of 0.15% level group, with no significant difference from 0.3% level group (P >0.05). In addition, there was no significant difference in the content of calcium and phosphorus in blood among the three groups (P>0.05), but the content of fecal calcium increased with the increase of calcium and phosphorus level (P<0.05). Transcriptome sequencing results showed that there was less difference between control group and 0.15% level group, but more significant difference between control group and 0.3% level group. And most of the differently expressed genes were enriched in sugar metabolism pathway, which was consistent with the quantitative PCR results. Also the activity of phosphorylases, the glucose metabolism-related enzyme, decreased with the increase of calcium and phosphorus levels (P<0.05). In a word, the level of calcium and phosphorus in the diet will affect the food intake of Magang geese, and it will also affect the sugar and lipid metabolism in the liver.

Key words: Magang goose, calcium and phosphorus level, growth performance, gene expression, metabolism

中图分类号:

S835

李虹仪, 周润盛, 梁笑玲, 张楚玥, 吕祺欣, 杨长华, 张茂. 日粮钙磷水平对马岗鹅生长性能及肝脏基因表达的影响[J]. 浙江农业学报, 2023, 35(11): 2533-2542.

LI Hongyi, ZHOU Runsheng, LIANG Xiaoling, ZHANG Chuyue, LYU Qixin, YANG Changhua, ZHANG Mao. Effect of dietary calcium and phosphorus level on Magang geese growth performance and liver gene expression[J]. Acta Agriculturae Zhejiangensis, 2023, 35(11): 2533-2542.

图/表 10

表1 基础日粮组成及营养水平

Table 1 Basic diet composition and nutrition level

日粮 Dietary	比例 Proportion/ %	营养素 Nutrient	营养水平 Nutritional level
豆粕 Soybean meal, CP 43%	4.50	代谢能 Metabolic energy/ (MJ·kg^-1)	11.37
皮大麦 Leather barley	7.00	粗蛋白含量 Crude protein content/%	10.31
小麦 Wheat	10.00	粗脂肪含量 Crude fat content/%	3.28
碎米 Broken rice	50.00	粗纤维含量 Crude fiber content/%	7.51
米糠 Rice bran	15.00	钙含量 Calcium content/%	0.26
统糠 Series chaff 预混料Premix	12.00 1.50	有效磷含量 Available phosphorus content/%	0.24
合计Total	100.00

图1 日粮不同钙水平下马岗鹅的采食量同一处理时间不同处理间没有相同字母表示差异显著(P<0.05)。

Fig.1 Feed intake of Magang geese under different dietary calcium levels The data of different treatments on the same treatment day without the same letters indicate the significant difference (P<0.05).

表2 日粮不同钙磷水平下马岗鹅的平均日增重及耗料增重比

Table 2 Average daily gain and feed/gain ratio of Magang geese under different dietary calcium and phosphorus levels

处理 Treatment	平均日增重 Average daily gain/g	耗料增重比 Feed/gain ratio
对照组Control group	44.64±16.17 a	6.09±2.56
0.15%磷酸氢钙组 0.15% calcium hydrogen phosphate addition group	27.09±6.43 b	6.33±1.37
0.3%磷酸氢钙组 0.3% calcium hydrogen phosphate addition group	34.09±15.72 ab	6.02±2.89

表3 日粮不同钙磷水平下马岗鹅的屠宰性能

Table 3 Slaughter performance of Magang geese under different dietary calcium and phosphorus levels kg

处理 Treatment	宰前活重 Weight	半净膛重 Semi-eviscerated weight	全净膛重 Eviscerated weight
对照组Control group	7.58±1.03 a	3.33±0.37 a	2.88±0.24 a
0.15%磷酸氢钙组 0.15% calcium hydrogen phosphate addition group	6.29±0.63 b	2.74±0.17 b	2.39±0.12 b
0.3%磷酸氢钙组 0.3% calcium hydrogen phosphate addition group	7.04±0.73 ab	3.12±0.23 ab	2.72±0.17 ab

表4 日粮不同钙磷水平下马岗鹅血液及粪便中钙磷的含量

Table 4 Calcium and phosphorus levels in blood and feces of Magang geese under different dietary calcium and phosphorus levels mmol·L-1

处理 Treatment	粪磷含量 Content of calcium in feces	粪钙含量 Content of phosphorus in feces	血钙含量 Blood calcium concentration	血磷含量 Blood phosphorus concentration
对照组Control group	5.48±1.31	3.34±1.41 b	2.92±0.26	4.35±1.68
0.15%磷酸氢钙组 0.15% calcium hydrogen phosphate addition group	6.13±1.09	4.12±1.64 b	3.10±0.29	4.81±1.91
0.3%磷酸氢钙组 0.3% calcium hydrogen phosphate addition group	5.10±1.85	7.23±3.58 a	3.13±0.28	4.33±1.08

图2 不同处理组间马岗鹅肝脏差异基因表达数量 L表示对照组,M表示0.15%磷酸氢钙组,H表示0.3%磷酸氢钙组,下同。

Fig.2 Number of differential gene expression in goose liver among different treatment groups L represents the control group, M represents the 0.15% calcium hydrogen phosphate addition group, and H represents the 0.3% calcium hydrogen phosphate addition group. The same as below.

图3 差异基因聚类图

Fig.3 Cluster diagram of differential genes

图4 差异基因富集通路图 A,0.3%磷酸氢钙组表达显著高于对照组基因所富集通路;B,0.3%磷酸氢钙组表达显著低于对照组基因所富集通路。

Fig.4 Enrichment pathway of differential genes A, Enrichment pathway of differential genes in the 0.3% calcium hydrogen phosphate addition group significantly higher than those in the control group; B, Enrichment pathway of differential genes in the 0.3% calcium hydrogen phosphate addition group significantly lower than those in the control group.

图5 0.3%磷酸氢钙组与对照组间前8个差异基因表达量 A,0.3%磷酸氢钙组表达显著高于对照组前8个基因的荧光定量PCR检测结果;B,0.3%磷酸氢钙组表达显著低于对照组前8个基因的荧光定量PCR检测结果。*、**分别表示与对照相比差异达显著(P<0.05)、极显著(P<0.01)水平。

Fig.5 Top 8 differential gene expressions between 0.3% calcium hydrogen addition group and control group A, Top 8 differential genes in the 0.3% calcium hydrogen addition group significantly higher than those in the control group; B, Top 8 differential genes in the 0.3% calcium hydrogen addition group significantly lower than those in the control group. *, ** indicates the significant difference at the level of 0.05 or 0.01.

表5 日粮不同钙磷水平下马岗鹅肝脏中GP活性

Table 5 GP activity in liver of Magang geese under different dietary calcium and phosphorus levels

处理 Treatment	肝脏中GP活性 GP activity in liver/ (ng·mL^-1·g^-1)
对照组Control group	33.03±7.19 a
0.15%磷酸氢钙组 0.15% calcium hydrogen phosphate addition group	24.13±3.97 b
0.3%磷酸氢钙组 0.3% calcium hydrogen phosphate addition group	20.32±1.72 b

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日粮钙磷水平对马岗鹅生长性能及肝脏基因表达的影响

Effect of dietary calcium and phosphorus level on Magang geese growth performance and liver gene expression

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