Genetic diversity analysis of 30 fig varieties using capillary electrophoresis detection with fluorescent SSR markers

doi:10.3969/j.issn.1004-1524.2017.09.09

›› 2017, Vol. 29 ›› Issue (9): 1482-1488.DOI: 10.3969/j.issn.1004-1524.2017.09.09

• Horticultural Science • Previous Articles Next Articles

Genetic diversity analysis of 30 fig varieties using capillary electrophoresis detection with fluorescent SSR markers

GUO Zhengbing¹, HAN Baiming¹, GUO Qiang²

1.Jiangsu Polytechnical College of Agriculture and Forestry, Jurong 212400, China;
2. Jurong Tigerhill Fig Professional Cooperatives, Jurong 212400, China

Received:2017-04-20 Online:2017-09-20 Published:2017-09-27

Abstract

Abstract: In order to analyze the genetic diversity of the fig species collected from home and abroad, the capillary electrophoresis detection with fluorescent SSR markers method was used to establish the fingerprint identification platform of 30 fig varieties. Analysis of 30 fig varieties was performed by using 15 pairs of SSR primers. The results showed that a total of 79 alleles were detected, the PIC value of 15 loci was between 0.332 8 and 0.875 7 with an average of 0.530 3 and the Shannon information index (I) of each point was between 0.604 9 to 2.127 3 with an average of 1.136 0. The genetic distance between the 30 varieties was 0.025 4-1.123 4. Among them, the heterozygosity and PIC value of primer FCUP038-6 were up to 0.846 8 and 0.861 1, respectively, which could be considered as the core marker of the purity of the hybrid test. Thirty fig varieties were rich in genetic diversity and were divided into two groups with different origins. There was no significant correlation between the genetic relationships and the origins of the varieties, as well as the phenotype feature.

Key words: Ficus carica L, genetic diversity, SSR

CLC Number:

S663.3

GUO Zhengbing, HAN Baiming, GUO Qiang. Genetic diversity analysis of 30 fig varieties using capillary electrophoresis detection with fluorescent SSR markers[J]. , 2017, 29(9): 1482-1488.

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Genetic diversity analysis of 30 fig varieties using capillary electrophoresis detection with fluorescent SSR markers

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