Genome-wide identification and expression analysis of purple acid phosphatases genes in pepper

doi:10.3969/j.issn.1004-1524.2017.09.11

›› 2017, Vol. 29 ›› Issue (9): 1498-1505.DOI: 10.3969/j.issn.1004-1524.2017.09.11

• Horticultural Science • Previous Articles Next Articles

Genome-wide identification and expression analysis of purple acid phosphatases genes in pepper

PANG Xin¹, CHENG Yuan², GUO Qinwei³, ZHENG Jiaqiu⁴, WAN Hongjian^{2, *}

1. Suzhou Polytechnic Institute of Agriculture, Suzhou 215008, China;
2. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. Quzhou Academy of Agricultural Sciences, Quzhou 324000, China;
4. Jiangsu Coastal Area Institute of Agricultural Sciences, Yancheng 224002, China

Received:2017-01-13 Online:2017-09-20 Published:2017-09-27

Abstract

Abstract: Purple acid phosphatases (PAPs) are members of the metallo-phosphoesterases involved in a variety of physiological functions, especially phosphate acquisition in plants. In this paper, genome-wide identification and analysis of PAP gene family members in pepper were performed with homologues alignment method. These results found that the whole pepper genome contains at least 25 PAP genes with protein sequence length varying from 264 to 639 aa. Number of introns ranged from 1 to 12. They are distributed unevenly on 12 chromosomes, only had one tandem duplication. Phylogenetic relationships showed that PAP genes from three plants species (pepper, Arabidopsis and rice) can be separated into 3 groups, 8 subgroups, and the number of each group is variable. Six and twenty pairs of orthologous and homologous genes were found, indicating that the diversity of PAP genes occurred before the split of pepper, Arabidopsis and rice. Based on the RNA-seq database and qRT-PCR method, the expression analysis of different tissues and fruit development stages indicated that these genes had tissue and development stages specificity.

Key words: pepper, purple acid phosphatases, gene family, bioinformatics, expression analysis

CLC Number:

S641.3

PANG Xin, CHENG Yuan, GUO Qinwei, ZHENG Jiaqiu, WAN Hongjian. Genome-wide identification and expression analysis of purple acid phosphatases genes in pepper[J]. , 2017, 29(9): 1498-1505.

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Genome-wide identification and expression analysis of purple acid phosphatases genes in pepper

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