Cloning and characterization of LlMBF1a in lily induced by gray mold pathogen

doi:10.3969/j.issn.1004-1524.2018.12.06

›› 2018, Vol. 30 ›› Issue (12): 2024-2030.DOI: 10.3969/j.issn.1004-1524.2018.12.06

• Horticultural Science • Previous Articles Next Articles

Cloning and characterization of LlMBF1a in lily induced by gray mold pathogen

CAO Xing¹, ZHANG Xiusheng¹, HOU Dong², SUI Juanjuan³, MU Hongmei¹, GAO Xiangbin¹, LYU Futang¹, GUO Shangjing¹, WANG Guiqing^1,*

1. College of Agriculture, Liaocheng University, Liaocheng 252059, China;
2. Vegetables Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China;
3. College of Biology and Food Engineering, Fuyang Normal College, Fuyang 236037, China

Received:2018-03-12 Online:2018-12-25 Published:2018-12-28

Abstract

Abstract: Previous studies revealed that MBF1a was involved in host defense response. The MBF1a homologous gene was isolated from lily cultivar White Heaven by silico cloning and RT-PCR approaches. The open reading frame of LlMBF1a gene was 429 bp, encoding a protein of 142 amino acid residues, and the molecular weight of LlMBF1a protein was 15.53 ku with a theoretical isoelectric point of 10.12. Bioinformatics analysis predicted that LlMBF1a was a stable soluble non-secreted protein, containing a typical MBF1 motif and a helix-turn-helix motif. Subcellular localization assay indicated LlMBF1a expressed in nucleus. The expression of LlMBF1a was at a similar level in leaf, stem, bulb and root, and was induced by Botrytis cinerea. The up-regulation of LlMBF1a in resistant variety was higher and more persistent than that in susceptible variety, suggesting LlMBF1a might be correlated to the resistance of lily against gray mold.

Key words: lily, MBF1, Botrytis cinerea, gene cloning, subcellular localization, gene expression

CLC Number:

S682.2⁺65

CAO Xing, ZHANG Xiusheng, HOU Dong, SUI Juanjuan, MU Hongmei, GAO Xiangbin, LYU Futang, GUO Shangjing, WANG Guiqing. Cloning and characterization of LlMBF1a in lily induced by gray mold pathogen[J]. , 2018, 30(12): 2024-2030.

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Cloning and characterization of LlMBF1a in lily induced by gray mold pathogen

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