Spatiotemporal expression profiling of two P450 genes, CYP4M38 and CYP4M39, in rice stem borer, Chilo suppressalis (Lepidoptera: Crambidae)

doi:10.3969/j.issn.1004-1524.2018.04.01

›› 2018, Vol. 30 ›› Issue (4): 521-527.DOI: 10.3969/j.issn.1004-1524.2018.04.01

• Plant Protection • Previous Articles Next Articles

Spatiotemporal expression profiling of two P450 genes, CYP4M38 and CYP4M39, in rice stem borer, Chilo suppressalis (Lepidoptera: Crambidae)

BAI Qi^{1, 2}, LU Yanhui², ZHENG Xusong², LYU Zhongxian^{2, *}

1.College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China;
2. State Key Laboratory of Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2017-07-01 Online:2018-04-20 Published:2018-04-19

Abstract

Abstract: Cytochrome P450 plays an important role in insect metabolism of exogenous substances, which is an important detoxification system of insects. The CYP4 family is an important branch of the insect cytochrome P450 gene family, which is closely related to the detoxification and metabolism of insects. In this study, the sequence homology and spatiotemporal expression profiles of CsCYP4M38 and CsCYP4M39 were investigated in order to clarify the expression information of CYP4 family gene in Chilo suppressalis. The results showed that CsCYP4M38 and CsCYP4M39 had highest sequence homology to MsCYP4M2 (56.86%) and MsCYP4M1 (59.96%), respectively. The phylogenetic tree showed that CsCYP4M38 and CsCYP4M39 belonged to the CYP4 family. The results of spatiotemporal expression profiling showed that the expression patterns of two genes in different developmental stages and different tissues were different. The CsCYP4M38 was expressed higher in the second instar larvae, 5^th instar larvae and female adults, but lower in the 1^st instar larvae. The CsCYP4M39 expressed higher in the larval stage, and the expression of CsCYP4M39 increased and then decreased. The expression level of CsCYP4M39 was lower in the egg, pupae and adult of C. suppressalis. The expression levels of CsCYP4M38 and CsCYP4M39 in the fat body, midgut and epidermis were relatively high, and both expressed highest in the fat body. The expression of CsCYP4M38 and CsCYP4M39 in different stages showed variability, which indicated that these two genes had different functions in different developmental stages of C. suppressalis, and both genes were expressed highest in the fat body, indicating the fat body might be the main detoxification tissue of insects.

Key words: Chilo suppressalis, cytochrome P450, CsCYP4M38, CsCYP4M39, gene expression

CLC Number:

S435.112⁺.1

BAI Qi, LU Yanhui, ZHENG Xusong, LYU Zhongxian. Spatiotemporal expression profiling of two P450 genes, CYP4M38 and CYP4M39, in rice stem borer, Chilo suppressalis (Lepidoptera: Crambidae)[J]. , 2018, 30(4): 521-527.

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Spatiotemporal expression profiling of two P450 genes, CYP4M38 and CYP4M39, in rice stem borer, Chilo suppressalis (Lepidoptera: Crambidae)

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