Study on extraction of Polygonatum sibiricum polysaccharides and its protective effect on CCl4-induced acute liver injury in rats

doi:10.3969/j.issn.1004-1524.2018.04.03

›› 2018, Vol. 30 ›› Issue (4): 537-547.DOI: 10.3969/j.issn.1004-1524.2018.04.03

• Animal Science • Previous Articles Next Articles

Study on extraction of Polygonatum sibiricum polysaccharides and its protective effect on CCl₄-induced acute liver injury in rats

HAN Chunyang, YANG Mingchuan, YANG Zisheng, FENG Kangli, LIU Cuiyan^*

College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received:2017-08-14 Online:2018-04-20 Published:2018-04-19

Abstract

Abstract: The present study was designed to investigate optimum extraction process of Polygonatum sibiricum polysaccharides (PSP) and explore its protective effect on carbon tetrachloride (CCl₄) induced liver injury in rats. Firstly, single factor experiment was carried out on the three main factors, including the ratio of liquid to material, time and temperature. Then, taking PSP extraction rate as response value, the extraction process was optimized by response surface analysis. Finally, the PSP was used to protect against CCl₄-induced liver injury in rats. The experiment rats were randomly divided into 5 groups: control group, model group, low dose, middle dose and high dose (75, 150, 300 mg·kg^-1) of PSP groups. The rats of PSP groups were administered with different doses of PSP by gavage. Control group and model group were fed with the same amount of distilled water. All rats were fed for 7 d. One hour after the last administration, the rats in the model group and PSP groups were injected intraperitoneally with 50% CCl₄ oil solution at the dose of 1 mL·kg^-1,and rats in the control group were received the same amount of soybean oil. Blood and liver samples were collected 24 h later after the last administration to test the pathological and histological changes of liver, the activities of ALT (alanine aminotransferase), AST (aspartate aminotransferase), ALP (alkaline phosphatase;) and the content changes of DBIL (direct bilirubin), TBIL (total bilirubin). The results showed that the optimum extraction conditions of PSP were as follows: liquid material ratio was 19.13:1, extraction time was 3.49 h and temperature was 73.33 ℃. Under these conditions, the actual PSP extraction rate was 10.107%. Compared with the model group, the middle dose PSP group and high dose PSP group could significantly (P<0.05) reduce the activities of ALT, AST, ALP and the contents of DBIL, TBIL in serum of rats. All doses of PSP could alleviate the pathological and histological changes of liver in different degrees, and the effect of high dose of PSP was the best. These results indicated that PSP had protective effect on CCl₄-induced liver injury in rats.

Key words: Polygonatum sibiricum polysaccharides, extraction, rat, liver injury

CLC Number:

S853.74

HAN Chunyang, YANG Mingchuan, YANG Zisheng, FENG Kangli, LIU Cuiyan. Study on extraction of Polygonatum sibiricum polysaccharides and its protective effect on CCl₄-induced acute liver injury in rats[J]. , 2018, 30(4): 537-547.

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Study on extraction of Polygonatum sibiricum polysaccharides and its protective effect on CCl₄-induced acute liver injury in rats

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