Cultural characteristics of Cordyceps javanica strain ZN-S21 and its indoor insecticidal activity against Bactrocera tau

doi:10.3969/j.issn.1004-1524.20250376

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (1): 95-104.DOI: 10.3969/j.issn.1004-1524.20250376

• Plant Protection • Previous Articles Next Articles

Cultural characteristics of Cordyceps javanica strain ZN-S21 and its indoor insecticidal activity against Bactrocera tau

WANG Luyao¹(), ZHOU Xiangyi¹, WANG Guorong², CHEN Mengli¹, WU Huiming¹^,^*()

1. Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China
2. Agricultural and Forestry Technology Extension Center of Xiaoshan District, Hangzhou City, Hangzhou 311699, China

Received:2025-05-14 Online:2026-01-25 Published:2026-02-11

Abstract

Abstract:

Bactrocera tau is a crucial quarantine pest. To clarify the biocontrol potential of the strain ZN-S21, which was isolated from the field, against B. tau, this study first identified the taxonomic status of strain ZN-S21 through morphological observations and multi-gene phylogenetic tree analysis. Subsequently, the cultural characteristics of strain ZN-S21 were systematically investigated, and its insecticidal activity against the 3rd instar larvae of B. tau under different environmental conditions was determined in the laboratory. The results showed that strain ZN-S21 was identified as Cordyceps javanica. When cultured on potato sucrose agar (PSA) medium, this strain exhibited a relatively fast colony growth rate and a high sporulation quantity under the conditions of 30 ℃ and pH value of 5.0. Additionally, the optimal conditions for the laboratory-based biocontrol of B. tau by C. javanica strain ZN-S21 were determined as follows: temperature of 30 ℃, spore concentration of 1×10⁸ mL^-1, soil relative water content of 90%, and soil pH value of 7.0. In conclusion, C. javanica strain ZN-S21 demonstrates strong insecticidal activity against the 3rd instar larvae of B. tau, thereby holding great potential for application in the population control of B. tau.

Key words: Bactrocera tau, Cordyceps javanica, insecticidal activity, temperature, soil relative water content

CLC Number:

S433

WANG Luyao, ZHOU Xiangyi, WANG Guorong, CHEN Mengli, WU Huiming. Cultural characteristics of Cordyceps javanica strain ZN-S21 and its indoor insecticidal activity against Bactrocera tau[J]. Acta Agriculturae Zhejiangensis, 2026, 38(1): 95-104.

Figures/Tables 10

References 34

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基因 Gene	引物名称 Primer name	序列(5'→3') Sequence (5'→3')
ITS^[18]	ITS5	GGAAGTAAAAGTCGTAACAAGG
	ITS4	TCCTCCGCTTATTGATATGC
LSU^[19]	LR5	ATCCTGAGGGAAACTTC
	LROR	GTACCCGCTGAACTTAAGC
TEF1^[20]	985F	GCTCCYGGHCAYCGTGAYTTYAT
	2218R	ATGACACCRACRGCRACRGTYTG
RPB1^[21]	CRPB1	CCWGGYTTYATCAAGAARGT
	RPB1Cr	CCNGCDATNTCRTTRTCCATRTA

基因 Gene	引物名称 Primer name	序列(5'→3') Sequence (5'→3')
ITS^[18]	ITS5	GGAAGTAAAAGTCGTAACAAGG
	ITS4	TCCTCCGCTTATTGATATGC
LSU^[19]	LR5	ATCCTGAGGGAAACTTC
	LROR	GTACCCGCTGAACTTAAGC
TEF1^[20]	985F	GCTCCYGGHCAYCGTGAYTTYAT
	2218R	ATGACACCRACRGCRACRGTYTG
RPB1^[21]	CRPB1	CCWGGYTTYATCAAGAARGT
	RPB1Cr	CCNGCDATNTCRTTRTCCATRTA

编号No.	A/℃	B/mL^-1	C/%	D	Y/%
1	20	1×10⁶	30	5.0	26.14±1.97 d
2	20	1×10⁷	60	7.0	37.50±5.21 c
3	20	1×10⁸	90	9.0	27.27±3.94 d
4	25	1×10⁶	60	9.0	26.14±1.97 d
5	25	1×10⁷	90	5.0	55.68±0.00 ab
6	25	1×10⁸	30	7.0	28.41±3.41 d
7	30	1×10⁶	90	7.0	60.23±1.97 a
8	30	1×10⁷	30	9.0	53.41±1.97 b
9	30	1×10⁸	60	5.0	38.64±0.01 c
k₁	30.30	37.50	35.98	37.12
k₂	36.74	48.86	31.06	42.05
k₃	47.73	28.41	47.73	35.61
R	17.43	20.45	16.67	6.44

编号No.	A/℃	B/mL^-1	C/%	D	Y/%
1	20	1×10⁶	30	5.0	26.14±1.97 d
2	20	1×10⁷	60	7.0	37.50±5.21 c
3	20	1×10⁸	90	9.0	27.27±3.94 d
4	25	1×10⁶	60	9.0	26.14±1.97 d
5	25	1×10⁷	90	5.0	55.68±0.00 ab
6	25	1×10⁸	30	7.0	28.41±3.41 d
7	30	1×10⁶	90	7.0	60.23±1.97 a
8	30	1×10⁷	30	9.0	53.41±1.97 b
9	30	1×10⁸	60	5.0	38.64±0.01 c
k₁	30.30	37.50	35.98	37.12
k₂	36.74	48.86	31.06	42.05
k₃	47.73	28.41	47.73	35.61
R	17.43	20.45	16.67	6.44

孢子浓度 Spore concentration/mL^-1	累计校正死亡率Corrected accumulative mortality/%					LT₅₀/d
孢子浓度 Spore concentration/mL^-1	3 d	5 d	7 d	10 d	15 d	LT₅₀/d
1.0×10⁴	4.44±3.85 b	5.75±1.99 d	10.34±3.45 d	16.09±3.98 e	18.97±7.31 e	41.72±13.77 a
1.0×10⁵	6.67±0 b	9.20±1.99 d	33.33±1.99 c	51.72±0.01 d	51.72±0.01 d	12.61±0.10 b
1.0×10⁶	6.67±0 b	22.99±1.99 c	39.08±1.99 b	63.22±1.99 c	65.52±0.01 c	10.08±0.09 b
1.0×10⁷	11.11±1.92 a	28.74±1.99 b	42.53±1.99 b	67.82±1.99 b	72.41±0.01 b	9.05±0.10 b
1.0×10⁸	13.33±0 a	35.63±1.99 a	51.72±3.45 a	79.31±0.01 a	84.48±2.44 a	7.50±0.12 b

Cultural characteristics of Cordyceps javanica strain ZN-S21 and its indoor insecticidal activity against Bactrocera tau

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t/d	LC₅₀/mL^-1	LC₅₀的95%置信区间/mL^-1 95% Confidence interval of LC₅₀/mL^-1
3	3.669×10⁸	6.970×10⁵~1.931×10⁸
5	7.468×10⁸	1.171×10⁸~4.763×10⁹
7	3.039×10⁷	2.403×10⁶~3.844×10⁸
10	4.655×10⁵	1.022×10⁵~2.120×10⁶
15	2.945×10⁵	9.378×10⁴~9.251×10⁵

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