Effects of intercropping with soybean and alfalfa on the rhizosphere soil microbial community structure of Paeonia rockii

doi:10.3969/j.issn.1004-1524.20250154

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (2): 351-363.DOI: 10.3969/j.issn.1004-1524.20250154

• Environmental Science • Previous Articles Next Articles

Effects of intercropping with soybean and alfalfa on the rhizosphere soil microbial community structure of Paeonia rockii

YANG Yushan¹(), ZHANG Miaomiao¹, LYU Zengwei², CHEN Xue¹, HUANG Qiuliang¹, LIU Haoyang¹, CAO Minghui¹, HUANG Zhenbei¹, ZHANG Guofang¹^,^*()

1. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2. Institute of Forestry and Grassland Science, Liangshan Yi Autonomous Prefecture, Xichang 615050, Sichuan, China

Received:2025-03-06 Online:2026-02-25 Published:2026-03-24

Abstract

Abstract:

This study aimed to investigate the effects of monocropping of Paeonia rockii and its intercropping with Glycine max and Medicago sativa L., respectively, on the rhizosphere soil microbial community structure, providing a theoretical basis for optimizing the ecological planting patterns of P. rockii. Using P. rockii monoculture as the control, treatments of P. rockii-G. max intercropping and P. rockii-M. sativa intercropping were established. Rhizosphere soil samples were collected, and high-throughput sequencing technology was used to analyze the composition and diversity of bacterial and fungal communities. The results indicated that, with the exception of available potassium, intercropping with soybean or alfalfa had no significant effect on soil available nutrient content but altered the rhizosphere microbial community structure. Intercropping with alfalfa increased the richness of bacterial communities, intercropping with soybean had no significant effect on the richness and diversity of fungal communities. Additionally, intercropping with soybeam increased the relative abundance of beneficial microbial groups such as Proteobacteria, Actinobacteriota, and Basidiomycota, and reduced the relative abundance of the pathogenic fungus Fusarium. In conclusion, intercropping of P. rockii helped enhance the abundance of beneficial rhizosphere microbes and suppressed pathogen enrichment. Intercropping with soybean showed greater advantages in improving the fungal community structure, making it more conducive to improving the rhizosphere soil micro-ecological environment of P. rockii.

Key words: Paeonia rockii, intercropping, rhizosphere soil, microbial community, richness, diversity

CLC Number:

YANG Yushan, ZHANG Miaomiao, LYU Zengwei, CHEN Xue, HUANG Qiuliang, LIU Haoyang, CAO Minghui, HUANG Zhenbei, ZHANG Guofang. Effects of intercropping with soybean and alfalfa on the rhizosphere soil microbial community structure of Paeonia rockii[J]. Acta Agriculturae Zhejiangensis, 2026, 38(2): 351-363.

Figures/Tables 12

References 39

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处理 Sample	pH值 pH value	SOM/(g·kg^-1)	AN/(mg·kg^-1)	AP/(mg·kg^-1)	AK/(mg·kg^-1)
CK	8.80±0.08 a	11.69±0.48 a	28.31±2.10 a	10.01±4.26 a	7.38±0.06 a
DD	8.84±0.03 a	11.76±0.47 a	41.69±8.82 a	8.69±0.52 a	6.99±0.21 b
MX	8.78±0.04 a	12.32±0.61 a	29.56±9.57 a	5.66±1.64 a	7.39±0.08 a

处理 Sample	pH值 pH value	SOM/(g·kg^-1)	AN/(mg·kg^-1)	AP/(mg·kg^-1)	AK/(mg·kg^-1)
CK	8.80±0.08 a	11.69±0.48 a	28.31±2.10 a	10.01±4.26 a	7.38±0.06 a
DD	8.84±0.03 a	11.76±0.47 a	41.69±8.82 a	8.69±0.52 a	6.99±0.21 b
MX	8.78±0.04 a	12.32±0.61 a	29.56±9.57 a	5.66±1.64 a	7.39±0.08 a

微生物 Microorganism	样品 Sample	丰富度指数Richness index		多样性指数Diversity index		测序深度指数 Coverage index
微生物 Microorganism	样品 Sample	ACE指数 ACE index	Chao 1指数 Chao 1 index	Simpson指数 Simpson index	Shannon指数 Shannon index	测序深度指数 Coverage index
细菌Bacteria	CK	3 499.03±11.14 b	3 501.64±16.05 b	0.99±0.01 a	10.76±0.00 a	0.99±0.01 a
	DD	3 117.07±420.63 b	3 114.06±424.58 b	0.99±0.01 a	10.50±0.13 a	0.96±0.02 a
	MX	4 108.92±338.89 a	4 128.54±346.57 a	0.99±0.01 a	10.79±0.30 a	0.95±0.01 a
真菌Fungi	CK	865.56±33.97 a	862.89±30.56 a	0.97±0.01 a	6.66±0.36 a	0.99±0.02 a
	DD	1 102.47±178.82 a	1 105.68±179.39 a	0.96±0.01 a	6.68±0.36 a	0.98±0.04 a
	MX	887.36±227.45 a	889.43±226.88 a	0.89±0.05 a	5.42±0.62 a	0.99±0.01 a

微生物 Microorganism	样品 Sample	丰富度指数Richness index		多样性指数Diversity index		测序深度指数 Coverage index
微生物 Microorganism	样品 Sample	ACE指数 ACE index	Chao 1指数 Chao 1 index	Simpson指数 Simpson index	Shannon指数 Shannon index	测序深度指数 Coverage index
细菌Bacteria	CK	3 499.03±11.14 b	3 501.64±16.05 b	0.99±0.01 a	10.76±0.00 a	0.99±0.01 a
	DD	3 117.07±420.63 b	3 114.06±424.58 b	0.99±0.01 a	10.50±0.13 a	0.96±0.02 a
	MX	4 108.92±338.89 a	4 128.54±346.57 a	0.99±0.01 a	10.79±0.30 a	0.95±0.01 a
真菌Fungi	CK	865.56±33.97 a	862.89±30.56 a	0.97±0.01 a	6.66±0.36 a	0.99±0.02 a
	DD	1 102.47±178.82 a	1 105.68±179.39 a	0.96±0.01 a	6.68±0.36 a	0.98±0.04 a
	MX	887.36±227.45 a	889.43±226.88 a	0.89±0.05 a	5.42±0.62 a	0.99±0.01 a

细菌 Bacterium	占比Proportion			真菌 Fungus	占比Proportion
细菌 Bacterium	CK	DD	MX	真菌 Fungus	CK	DD	MX
Vicinamibacteraceae	4.69	2.62	2.83	拟鬼伞属Coprinopsis	0	9.46	0
鞘氨醇单胞菌属Sphingomonas	2.80	2.52	2.72	异茎点霉属Paraphoma	0	1.99	0
MND1	2.85	2.57	2.66	锥盖伞属Conocybe	0	1.50	0
WD2101_soil_group	4.33	1.40	2.97	被孢霉属Mortierella	0	1.37	0
KD4-96	1.61	2.30	2.01	毛壳菌属Chaetomium	0	4.48	0
Gitt-GS-136	1.04	2.12	1.61	四枝孢属Tetracladium	1.78	3.34	2.47
盖氏菌属Gaiella	1.64	1.67	1.18	镰刀菌属Fusarium	2.50	1.07	2.17
MB-A2-108	1.35	1.32	1.12	外瓶霉属Exophiala	3.85	5.22	3.98
RB41	2.80	0	1.81	膜座霉属Hymenula	3.96	2.09	1.61
小梨形菌属Pirellula	0	1.39	1.08	枝孢属Cladosporium	1.87	0	1.33
Pir4_lineage	0	1.43	1.37	拟折孢属Minimelanolocus	1.57	1.55	0
Subgroup_7	0	1.46	1.32	斑褶菇属Panaeolus	1.75	0	0
出芽菌属Gemmata	0	1.54	1.24	暗孔菌属Amaurodon	1.70	0	2.78
Pedosphaeraceae	1.69	1.05	1.05	小荚孢腔菌属Sporormiella	0	3.46	0
绿弯菌属Chloroflexus	0	0	4.26	球毛壳孢属Chaetosphaeronema	0	0	1.52
TRA3-20	1.67	1.04	0	脐菇属Hebeloma	7.53	0	4.37
Rokubacteriales	1.15	0	0	地孔菌属Geopora	2.30	0	0
东秀珠氏菌属Dongia	1.39	1.18	0	绒革菌属Tomentella	3.25	0	2.56
土生单胞菌属Terrimonas	1.14	0	0
Ellin6067	1.17	0	0
黄色土杆菌属Chthoniobacter	1.29	0	0
IMCC26256	1.04	0	0
Reyranella	0	1.07	0

Effects of intercropping with soybean and alfalfa on the rhizosphere soil microbial community structure of Paeonia rockii

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类群 Group	占比Proportion
类群 Group	CK	DD	MX
凋落物腐生-外生菌根-枯叶腐生-未知腐生真菌	21.94	7.05	5.17
Dung saprotroph-ectomycorrhizal-litter saprotroph-undefined saprotroph
外生菌根真菌Ectomycorrhizal	21.18	0	21.58
凋落物腐生-植物腐生-木质腐生真菌Dung saprotroph-plant saprotroph-wood saprotroph	0	16.94	1.52
动物病原-未知腐生真菌Animal pathogen-undefined saprotroph	5.58	9.09	8.44
动物病原-凋落物腐生-内生-附生-植物腐生-木质腐生真菌	0	7.78	0
Animal pathogen-dung saprotroph-endophyte-epiphyte-plant saprotroph-wood saprotroph
植物病原菌Plant pathogen	7.56	6.10	5.85
真菌寄生-植物病原-植物腐生真菌Fungal parasite-plant pathogen-plant saprotroph	2.64	4.28	6.24
动物病原-内生-枯叶腐生-植物病原-土壤腐生-木质腐生真菌	3.58	1.87	4.57
Animal pathogen-endophyte-lichen parasite-plant pathogen-soil saprotroph-wood saprotroph
动物病原-内生-枯叶腐生-植物病原-木质腐生真菌	3.40	1.30	3.54
Animal pathogen-endophyte-lichen parasite-plant pathogen-wood saprotroph
动物病原-真菌寄生-未知腐生真菌Animal pathogen-fungal parasite-undefined saprotroph	2.38	3.18	2.09

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