Analysis of GRM1 gene polymorphism and its correlation with meat quality traits in sheep

doi:10.3969/j.issn.1004-1524.2023.04.07

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (4): 799-808.DOI: 10.3969/j.issn.1004-1524.2023.04.07

• Animal Science • Previous Articles Next Articles

Analysis of GRM1 gene polymorphism and its correlation with meat quality traits in sheep

NIE Wei(), MENG Ke, RONG Xuan, QIANG Hao, GUO Chenhao, TAO Maohai, FENG Dengzhen()

College of Agriculture, Ningxia University, Yinchuan 750021, China

Received:2022-05-26 Online:2023-04-25 Published:2023-05-05

Abstract

Abstract:

To investigate the polymorphism of metabotropic glutamate receptor 1 gene (GRM1) in sheep and its effect on meat quality traits, with a view to providing effective genetic molecular markers for the breeding of sheep for meat performance. The GRM1 gene was sequenced in 91 sheep from three sheep populations, Tan sheep (T), Little-tailed Han sheep (XH), and Dorper sheep (D), using liquid-phase capture sequencing technology. The genotypes of the genotyped loci were analyzed by flight mass spectrometry for the meat quality traits of the Tan sheep (T), Little-tailed Han sheep (XH), Dorper sheep (D) and Dutanhan sheep (DTH). The results showed that there were significant differences in meat quality traits among the different genotypes of sheep. Sheep kidney mass with CT genotype was significantly (P<0.05) greater than that with TT genotype at the rs415006419 locus of the GRM1 gene, and tyrosine content in muscle of sheep with TT genotype was significantly (P<0.05) higher than that with CT genotype; Sheep live weight before slaughter, carcass weight, net meat weight, duodenal length, and heart, lung and kidney mass with TT genotype at locus rs403075278 of the GRM1 gene were significantly (P<0.01) greater than those with the CT genotype, and backfat thickness, net meat weight, spleen mass, muscle fat, calcium content, phenylalanine and histidine content with TT genotype were significantly (P<0.05) higher than those with CT genotype. pH, shear and water loss with CT genotype were significantly (P<0.01) greater than those with TT genotype. The remaining slaughter performance, meat quality and muscle nutrient content were not significantly (P>0.05) different between the two genotypes. The above results suggest that GRM1 gene could be used as a candidate gene for meat quality traits in sheep, rs415006419 and rs403075278 loci could be used as potential candidate genetic markers for sheep growth and development, and meat production performance.

Key words: sheep, metabotropic glutamate receptor 1, polymorphism, slaughter performance, meat trait, nutrient

CLC Number:

S826

NIE Wei, MENG Ke, RONG Xuan, QIANG Hao, GUO Chenhao, TAO Maohai, FENG Dengzhen. Analysis of GRM1 gene polymorphism and its correlation with meat quality traits in sheep[J]. Acta Agriculturae Zhejiangensis, 2023, 35(4): 799-808.

Figures/Tables 9

References 36

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SNPs	突变类型 Mutation type	上游引物 Forward primer(5’→3’)	下游引物 Reverse primer (5’→3’)	延伸引物 Extended primer (5’→3’)
rs415006419	同义突变 Synonymous mutation	ACGTTGGATGGAAAGGGAGAAAGAGATAG	ACGTTGGATGCTTCTGCGAAGGGATGACTG	TGGGCGAGTTCTCACTCAT
rs403075278	同义突变 Synonymous mutation	ACGTTGGATGTGCAGCAGGTTCTGTACTTG	ACGTTGGATGCAGGACTAAGAAGCCCATTG	TGGGGCGTGATCGGCCC

SNPs	突变类型 Mutation type	上游引物 Forward primer(5’→3’)	下游引物 Reverse primer (5’→3’)	延伸引物 Extended primer (5’→3’)
rs415006419	同义突变 Synonymous mutation	ACGTTGGATGGAAAGGGAGAAAGAGATAG	ACGTTGGATGCTTCTGCGAAGGGATGACTG	TGGGCGAGTTCTCACTCAT
rs403075278	同义突变 Synonymous mutation	ACGTTGGATGTGCAGCAGGTTCTGTACTTG	ACGTTGGATGCAGGACTAAGAAGCCCATTG	TGGGGCGTGATCGGCCC

位点 Locus	物理位置 Physical location	突变频数Mutation frequency						群体 Group	基因型频率 Genotype frequency/%				基因频率 Gene frequency/%
		D		XH		T			基因型频率 Genotype frequency/%				基因频率 Gene frequency/%
				Het	Hom	Het	Hom		Het	Hom		CC	CT	TT	χ²	C	T
rs415006419	77181965	4	26	8	21	13	17	D	0	13.30	86.70	10.30	6.70	93.30
								T	0	43.30	56.70		21.70	78.30
								XH	6.50	25.80	67.70		19.40	80.60
rs403075278	77332591	8	17	13	16	18	12	D	16.70	26.70	56.70	10.80	30.00	70.00
								T	0	60.00	40.00		30.00	70.00
								XH	6.50	41.90	51.60		27.40	72.60

位点 Locus	物理位置 Physical location	突变频数Mutation frequency						群体 Group	基因型频率 Genotype frequency/%				基因频率 Gene frequency/%
		D		XH		T			基因型频率 Genotype frequency/%				基因频率 Gene frequency/%
				Het	Hom	Het	Hom		Het	Hom		CC	CT	TT	χ²	C	T
rs415006419	77181965	4	26	8	21	13	17	D	0	13.30	86.70	10.30	6.70	93.30
								T	0	43.30	56.70		21.70	78.30
								XH	6.50	25.80	67.70		19.40	80.60
rs403075278	77332591	8	17	13	16	18	12	D	16.70	26.70	56.70	10.80	30.00	70.00
								T	0	60.00	40.00		30.00	70.00
								XH	6.50	41.90	51.60		27.40	72.60

位点	群体	He	Ho	Ne	PIC	P
Locus	Group
rs415006419	D	0.13	0.88	1.14	0.12	0.93
	T	0.42	0.58	1.72	0.33	0.14
	XH	0.34	0.66	1.51	0.28	0.32
rs403075278	D	0.42	0.58	1.72	0.33	0.14
	T	0.34	0.66	1.51	0.28	0.32
	XH	0.42	0.58	1.72	0.33	0.06

Analysis of GRM1 gene polymorphism and its correlation with meat quality traits in sheep

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位点 Locus	基因型 Genotype	宰前活重 Live weight/ kg	GR值 GR value/ cm	胴体重 Carcass weight/ kg	背膘厚 Back fat/ cm	屠宰率 Slaughter rate/%	净肉重 Net meat weight/ kg	净肉率 Net meat rate/ %	十二指肠长度 Duodenal length/ cm	质量 Weight/kg
位点 Locus	基因型 Genotype	宰前活重 Live weight/ kg	GR值 GR value/ cm	胴体重 Carcass weight/ kg	背膘厚 Back fat/ cm	屠宰率 Slaughter rate/%	净肉重 Net meat weight/ kg	净肉率 Net meat rate/ %	十二指肠长度 Duodenal length/ cm	心 Heart	肝 Liver	脾 Spleen	肺 Lung	肾 Kidney	全骨 Total bone
rs415006419	CT	26.69 ±6.20	0.75 ±0.27	12.75 ±3.90	0.37 ±0.18	47.25 ±5.25	9.64 ±3.52	35.37 ±5.87	17.00 ±3.31	0.12 ±0.02	0.37 ±0.09	0.06 ±0.03	0.35 ±0.13	0.22 ±0.11 a	3.11 ±0.53
	TT	27.68 ±5.49	0.81 ±0.31	12.38 ±2.88	0.31 ±0.13	44.65 ±4.81	9.21 ±2.64	33.13 ±5.69	16.79 ±2.61	0.13 ±0.02	0.39 ±0.10	0.05 ±0.02	0.42 ±0.15	0.14 ±0.08 b	3.16 ±0.81
rs403075278	CT	23.53 ±4.39 Bb	0.63 ±0.19	10.21 ±2.26 Bb	0.23 ±0.06 b	43.19 ±3.00	7.14 ±1.73 Bb	30.22 ±4.05 b	15.06 ±2.19 Bb	0.11± 0.02 Bb	0.33 ±0.08	0.04 ±0.02 b	0.27± 0.11 Bb	0.09± 0.07 Bb	3.07 ±0.96
	TT	29.42 ±5.32 Aa	0.86 ±0.30	13.97 ±3.36 Aa	0.37 ±0.17 a	47.32 ±5.71	10.71 ±3.02 Aa	36.16 ±5.86 a	17.82 ±2.83 Aa	0.13± 0.02 Aa	0.42 ±0.11	0.06 ±0.02 a	0.45± 0.12 Aa	0.22± 0.08 Aa	3.26 ±0.61

位点 Locus	基因型 Genotype	肌纤维直径 Muscle fiber diameter/μm	肌纤维密度 Muscle fiber density/mm^-2	pH值 pH value	剪切力 Shear force	熟肉率 Cooked meat rate/%	失水率 Water loss rate/%
rs415006419	CT	28.90±3.75	789.41±182.77	5.58±0.47	25.70±13.32	62.75±5.65	34.06±6.45
	TT	32.87±8.96	724.96±240.98	5.62±0.50	31.14±12.73	60.4±5.57	37.13±5.85
rs403075278	CT	32.22±3.66	734.56±172.22	6.08±0.45 Aa	42.67±14.53 Aa	61.17±7.26	40.76±5.06 Aa
	TT	31.05±7.92	738.68±228.79	5.44±0.40 Bb	21.92±13.21 Bb	62.75±5.64	33.44±5.27 Bb

位点 Locus	基因型 Genotype	脂肪 Fat/%	蛋白质 Protein/ %	肌酐 Creat- inine/ (μg· g^-1)	肌苷酸 Creatinine acid/ (μg· g^-1)	胆固醇 Inosinic acid/ (μg· g^-1)	铜 Cu/ (mg· kg^-1)	锌 Zn/ (mg· kg^-1)	硒 Se/ (mg· kg^-1)	钙 Ca/ (mg· kg^-1)	铁 Fe/ (mg· kg^-1)	锰 Mn/ (mg· kg^-1)	天冬氨酸 Aspartic acid/ (mg· g^-1)	苏氨酸 Threonine/ (mg· g^-1)	丝氨酸 Serine/ (mg· g^-1)
rs415006419	CT	3.46 ±1.84	20.90 ±1.34	450.34 ±98.30	1838.03 ±339.12	307.59 ±64.54	1.62 ±0.39	24.38 ±2.80	221.86 ±91.32	139.74 ±13.68	23.11 ±4.19	291.01 ±42.07	1.72 ±0.23	0.92 ±0.11	0.82 ±0.12
	TT	3.46 ±1.93	20.77 ±1.21	456.13 ±131.79	1978.06 ±350.81	313.07 ±56.14	1.42 ±0.47	25.75 ±3.83	195.03 ±82.99	142.94 ±31.95	23.25 ±4.17	285.15 ±27.30	1.86 ±0.39	0.99 ±0.19	0.86 ±0.17
rs403075278	CT	2.25 ±0.83 b	20.30 ±1.22	547.64 ±86.89	2054.80 ±292.85	290.24 ±70.96	1.51 ±0.39	26.33 ±1.81	174.92 ±20.87	126.84 ±23.80 b	24.27 ±3.90	283.74 ±18.41	1.73 ±0.42	0.94 ±0.20	0.80 ±0.18
	TT	4.07 ±1.92 a	21.22 ±1.21	437.73 ±160.29	1776.24 ±451.47	316.67 ±53.81	1.49 ±0.49	24.47 ±3.85	220.26 ±98.90	147.04 ±22.84 a	22.97 ±4.38	287.76 ±39.57	1.83 ±0.29	0.97 ±0.14	0.87 ±0.13
位点 Locus	基因型 Genotype	谷氨酸 Gluta- mate/ (mg· g^-1)	甘氨酸 Glycine/ (mg· g^-1)	丙氨酸 Alanine/ (mg· g^-1)	半胱氨酸 Cysteine/ (mg· g^-1)	缬氨酸 Valine/ (mg· g^-1)	甲硫氨酸 Methionine/ (mg· g^-1)	异亮氨酸 Isole- ucine/ (mg· g^-1)	亮氨酸 Leucine/ (mg· g^-1)	酪氨酸 Tyrosine/ (mg· g^-1)	苯丙氨酸 Phenyla- lanine/ (mg· g^-1)	赖氨酸 Lysine/ (mg· g^-1)	组氨酸 Histidine/ (mg· g^-1)	精氨酸 Argin- ine/ (mg· g^-1)	脯氨酸 Proline/ (mg· g^-1)
rs415006419	CT	2.90 ±0.37	0.83 ±0.11	1.27 ±0.23	0.03 ±0.06	1.00 ±0.17	0.49 ±0.13	0.96 ±0.13	1.68 ±0.18	0.67 ±0.14 b	1.26 ±0.21	1.95 ±0.29	0.88 ±0.20	1.16 ±0.11	1.10 ±0.22
	TT	3.17 ±0.65	0.90 ±0.17	1.30 ±0.26	0.05 ±0.08	1.13 ±0.35	0.62 ±0.35	1.08 ±0.31	1.87 ±0.42	0.83 ±0.23 a	1.32 ±0.27	2.07 ±0.37	0.90 ±0.21	1.30 ±0.24	1.02 ±0.31
rs403075278	CT	2.98 ±0.73	0.84 ±0.21	1.17 ±0.34	0.04 ±0.02	1.03 ±0.43	0.59 ±0.38	1.07 ±0.38	1.81 ±0.47	0.84 ±0.25	1.13 ±0.30 b	1.90 ±0.48	0.76 ±0.16 b	1.29 ±0.29	1.18 ±0.36
	TT	3.07 ±0.47	0.89 ±0.12	1.35 ±0.18	0.04 ±0.08	1.10 ±0.20	0.55 ±0.22	1.01 ±0.19	1.77 ±0.28	0.71 ±0.18	1.35 ±0.19 a	2.07 ±0.25	0.95 ±0.19 a	1.21 ±0.16	1.01 ±0.22

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