Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (7): 1511-1518.DOI: 10.3969/j.issn.1004-1524.20231326

• Animal Science • Previous Articles     Next Articles

Effects of Spirulina platensis and Haematococcus pluvialis on the growth performance, antioxidant enzymes, phosphatase, and heat shock protein of zebrafish (Danio rerio)

LI Fei1(), SU Tiantian2, SU Kangjie2, XU Ke2, MA Li2, LIU Ziming2,*()   

  1. 1. College of Ocean Engineering, Tangshan Maritime Institute, Tangshan 063200, Hebei, China
    2. College of Ecology, Lishui University, Lishui 323000, Zhejiang, China
  • Received:2023-11-24 Online:2024-07-25 Published:2024-08-05

Abstract:

Spirulina platensis and Haematococcus pluvialis are both high-quality microalgae with good nutritional and medicinal value. This study investigated the effects of dietary supplementation of 2% or 4% S. platensis and H. pluvialis on the growth performance, antioxidant enzyme and phosphatase activities, and heat shock protein expression of zebrafish (Danio rerio), which laid the foundation for the joint application of the two algae. The results showed that the final weight, weight gain and specific growth rates of zebrafish in group with 4% S. platensis and 2% H. pluvialis added to feed were significantly higher than those in the control group, and the gains were accompanied by significant increases in amylase and protease activities. The effects of dietary supplementation of two algae on the antioxidant enzyme activities of zebrafish were relatively complex. Compared with the control group, superoxide dismutase activities in two groups (2% S. platensis + 2% H. pluvialis and 4% S. platensis + 2% H. pluvialis) were significantly elevated. The opposite results were observed in catalase activities of zebrafish from two groups (4% S. platensis + 2% H. pluvialis and 4% S. platensis + 4% H. pluvialis). Zebrafish fed a diet supplemented with S. platensis and H. pluvialis had significant elevations in phosphatase activities and the expression of heat shock proteins. Phosphatase activities and heat shock protein 70 expression of zebrafish in all groups with algae supplementation were significantly higher than those in the control group, which illustrated that the combined feeding of S. platensis and H. pluvialis was conducive to improve the immune related levels and stress resistance of zebrafish. Compared with the control group, the significant elevation in the expression of heat shock protein 90 in zebrafish was detected in only one group (4% S. platensis + 2% H. pluvialis) rather than the other three groups. To sum up, dietary supplementation of S. platensis and H. pluvialis improved the growth performance, immune related level and anti-stress ability of zebrafish, and the addition with 4% S. platensis + 2% H. pluvialis was more appropriate.

Key words: Spirulina platensis, Haematococcus pluvialis, zebrafish, growth performance, antioxidant enzyme, phosp hatase, heat shock protein

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