Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (7): 1511-1518.DOI: 10.3969/j.issn.1004-1524.20231326
• Animal Science • Previous Articles Next Articles
LI Fei1(), SU Tiantian2, SU Kangjie2, XU Ke2, MA Li2, LIU Ziming2,*(
)
Received:
2023-11-24
Online:
2024-07-25
Published:
2024-08-05
CLC Number:
LI Fei, SU Tiantian, SU Kangjie, XU Ke, MA Li, LIU Ziming. Effects of Spirulina platensis and Haematococcus pluvialis on the growth performance, antioxidant enzymes, phosphatase, and heat shock protein of zebrafish (Danio rerio)[J]. Acta Agriculturae Zhejiangensis, 2024, 36(7): 1511-1518.
Add to citation manager EndNote|Ris|BibTeX
URL: http://www.zjnyxb.cn/EN/10.3969/j.issn.1004-1524.20231326
项目Item | 对照组CK | SP2-HP2 | SP2-HP4 | SP4-HP2 | SP4-HP4 | |
---|---|---|---|---|---|---|
成分 | 鱼粉Fish meal | 50 | 46 | 44 | 44 | 42 |
Component/(g·kg-1) | 螺旋藻Spirulina | 0 | 2 | 2 | 4 | 4 |
雨生红球藻Haematococcus pluvialis | 0 | 2 | 4 | 2 | 4 | |
小麦粉Wheat flour | 16 | 16 | 16 | 16 | 16 | |
豆粉Soybean flour | 16 | 16 | 16 | 16 | 16 | |
玉米淀粉Corn starch | 10 | 10 | 10 | 10 | 10 | |
鱼油Fish oil | 3 | 3 | 3 | 3 | 3 | |
豆油Soybean oil | 3 | 3 | 3 | 3 | 3 | |
多维预混物Multivitamin premix 1 | 1 | 1 | 1 | 1 | 1 | |
多矿预混物Multimineral premix 2 | 1 | 1 | 1 | 1 | 1 | |
生化组成 | 粗蛋白Crude protein | 45.54 | 45.50 | 45.43 | 45.31 | 45.09 |
Biochemical composition/% | 粗脂肪Crude fat | 9.08 | 9.15 | 9.21 | 9.24 | 9.28 |
灰分Ash | 7.65 | 7.67 | 7.73 | 7.74 | 7.69 | |
水分Water | 8.64 | 8.58 | 8.67 | 8.55 | 8.60 |
Table 1 Feed formula and biochemical composition for the experiment
项目Item | 对照组CK | SP2-HP2 | SP2-HP4 | SP4-HP2 | SP4-HP4 | |
---|---|---|---|---|---|---|
成分 | 鱼粉Fish meal | 50 | 46 | 44 | 44 | 42 |
Component/(g·kg-1) | 螺旋藻Spirulina | 0 | 2 | 2 | 4 | 4 |
雨生红球藻Haematococcus pluvialis | 0 | 2 | 4 | 2 | 4 | |
小麦粉Wheat flour | 16 | 16 | 16 | 16 | 16 | |
豆粉Soybean flour | 16 | 16 | 16 | 16 | 16 | |
玉米淀粉Corn starch | 10 | 10 | 10 | 10 | 10 | |
鱼油Fish oil | 3 | 3 | 3 | 3 | 3 | |
豆油Soybean oil | 3 | 3 | 3 | 3 | 3 | |
多维预混物Multivitamin premix 1 | 1 | 1 | 1 | 1 | 1 | |
多矿预混物Multimineral premix 2 | 1 | 1 | 1 | 1 | 1 | |
生化组成 | 粗蛋白Crude protein | 45.54 | 45.50 | 45.43 | 45.31 | 45.09 |
Biochemical composition/% | 粗脂肪Crude fat | 9.08 | 9.15 | 9.21 | 9.24 | 9.28 |
灰分Ash | 7.65 | 7.67 | 7.73 | 7.74 | 7.69 | |
水分Water | 8.64 | 8.58 | 8.67 | 8.55 | 8.60 |
基因 Gene | 引物序列 Primer sequence(5'-3') | GenBank编录号 GenBank accession No. | 产物长度 Product length/bp |
---|---|---|---|
HSP70 | F: CATGAAGAGCAGTGTGGAAGAC | AF210640 | 182 |
R: TGGAGATGACTGGATTGCAG | |||
HSP90 | F: CCCATTGTGGAGACCCTCAGA | AF068773 | 179 |
R: TCATCGATGCCAAGTCCGAG | |||
β-actin | F: CATGGCTTCTGCTCTGTATG | AF026063 | 143 |
R: GCAAAGTGGTAAACGCTTCT |
Table 2 Primer sequence used in the experiment
基因 Gene | 引物序列 Primer sequence(5'-3') | GenBank编录号 GenBank accession No. | 产物长度 Product length/bp |
---|---|---|---|
HSP70 | F: CATGAAGAGCAGTGTGGAAGAC | AF210640 | 182 |
R: TGGAGATGACTGGATTGCAG | |||
HSP90 | F: CCCATTGTGGAGACCCTCAGA | AF068773 | 179 |
R: TCATCGATGCCAAGTCCGAG | |||
β-actin | F: CATGGCTTCTGCTCTGTATG | AF026063 | 143 |
R: GCAAAGTGGTAAACGCTTCT |
处理 Treatment | 初始体重 Initial weight/g | 终末体重 Terminal weight/g | 体重增重率 Weight gain rate/% | 特定生长率 Specific growth rate/(%·d-1) | 成活率 Survival rate/% |
---|---|---|---|---|---|
CK | 0.025 5±0.001 2 a | 0.061 1±0.003 1 a | 139.5±10.2 a | 1.455±0.072 a | 96.27±2.89 a |
SP2-HP2 | 0.025 1±0.000 8 a | 0.072 1±0.010 5 ab | 186.8±36.9 ab | 1.747±0.215 bc | 98.33±2.89 a |
SP2-HP4 | 0.026 1±0.001 0 a | 0.066 8±0.006 7 ab | 156.0±17.3 a | 1.564±0.113 ab | 98.33±2.89 a |
SP4-HP2 | 0.026 0±0.000 4 a | 0.079 5±0.007 2 b | 205.7±22.4 b | 1.859±0.121 c | 100.00±0.00 a |
SP4-HP4 | 0.025 4±0.000 5 a | 0.071 0±0.009 9 ab | 179.8±37.2 ab | 1.705±0.214 abc | 98.33±2.89 a |
Table 3 Effects of S. platensis and H. pluvialis on growth performance of zebrafish
处理 Treatment | 初始体重 Initial weight/g | 终末体重 Terminal weight/g | 体重增重率 Weight gain rate/% | 特定生长率 Specific growth rate/(%·d-1) | 成活率 Survival rate/% |
---|---|---|---|---|---|
CK | 0.025 5±0.001 2 a | 0.061 1±0.003 1 a | 139.5±10.2 a | 1.455±0.072 a | 96.27±2.89 a |
SP2-HP2 | 0.025 1±0.000 8 a | 0.072 1±0.010 5 ab | 186.8±36.9 ab | 1.747±0.215 bc | 98.33±2.89 a |
SP2-HP4 | 0.026 1±0.001 0 a | 0.066 8±0.006 7 ab | 156.0±17.3 a | 1.564±0.113 ab | 98.33±2.89 a |
SP4-HP2 | 0.026 0±0.000 4 a | 0.079 5±0.007 2 b | 205.7±22.4 b | 1.859±0.121 c | 100.00±0.00 a |
SP4-HP4 | 0.025 4±0.000 5 a | 0.071 0±0.009 9 ab | 179.8±37.2 ab | 1.705±0.214 abc | 98.33±2.89 a |
Fig.1 Effects of S. platensis and H. pluvialis on AMS (A), LPS (B), and PRS (C) activities of zebrafish The bars with different letters indicate significant (P<0.05) differences among different experimental groups. The same as below.
[1] | ROY S S, PAL R. Microalgae in aquaculture: a review with special references to nutritional value and fish dietetics[J]. Proceedings of the Zoological Society, 2015, 68(1): 1-8. |
[2] | DE LIMA VALENÇA R, DA SILVA SOBRINHO A G, SILVA L G, et al. Marine algae meal (Schizochytrium sp.) and vitamin E in lamb nutrition: intake, digestibility, nitrogen balance, ingestive behavior, ruminal parameters, performance, and carcass characteristics[J]. Livestock Science, 2022, 258: 104877. |
[3] | LUPATINI A L, COLLA L M, CANAN C, et al. Potential application of microalga Spirulina platensis as a protein source[J]. Journal of the Science of Food and Agriculture, 2017, 97(3): 724-732. |
[4] | SHALATA H A, BAHATTAB O, ZAYED M M, et al. Synergistic effects of dietary sodium butyrate and Spirulina platensis on growth performance, carcass composition, blood health, and intestinal histomorphology of Nile tilapia (Oreochromis niloticus)[J]. Aquaculture Reports, 2021, 19: 100637. |
[5] | RÉGNIER P, BASTIAS J, RODRIGUEZ-RUIZ V, et al. Astaxanthin from Haematococcus pluvialis prevents oxidative stress on human endothelial cells without toxicity[J]. Marine Drugs, 2015, 13(5): 2857-2874. |
[6] | POGORZELSKA E, GODZISZEWSKA J, BRODOWSKA M, et al. Antioxidant potential of Haematococcus pluvialis extract rich in astaxanthin on colour and oxidative stability of raw ground pork meat during refrigerated storage[J]. Meat Science, 2018, 135: 54-61. |
[7] | LAWRENCE C. The husbandry of zebrafish (Danio rerio): a review[J]. Aquaculture, 2007, 269(1/2/3/4): 1-20. |
[8] | HARDY R. Zebrafish as a model fish in nutrition research[J]. Aquaculture Magazine, 2007, 33(4): 81-83. |
[9] | GEFFROY B, SIMON O. Effects of a Spirulina platensis-based diet on zebrafish female reproductive performance and larval survival rate[J]. Cybium: International Journal of Ichthyology, 2013, 37(1/2): 31-38. |
[10] | SEENIVASAN C, RADHAKRISHNAN S, MURALISANKAR T, et al. Effects of probiotics on survival, growth and digestive enzymes activities in freshwater prawn Macrobrachium rosenbergii(de man 1879)[J]. Proceedings of the Zoological Society, 2016, 69(1): 52-60. |
[11] | YUAN Y, JIN M, XIONG J, et al. Effects of dietary dosage forms of copper supplementation on growth, antioxidant capacity, innate immunity enzyme activities and gene expressions for juvenile Litopenaeus vannamei[J]. Fish & Shellfish Immunology, 2019, 84: 1059-1067. |
[12] | SAYED A E D H, HAMED M, SOLIMAN H A M. Spirulina platensis alleviated the hemotoxicity, oxidative damage and histopathological alterations of hydroxychloroquine in catfish (Clarias gariepinus)[J]. Frontiers in Physiology, 2021, 12: 683669. |
[13] | UMPHAN K M. Growth performance, sex hormone levels and maturation ability of Pla Pho (Pangasius bocourti) fed with Spirulina supplementary pellet and hormone application[J]. International Journal of Agriculture and Biology, 2009, 11(4): 458-462. |
[14] | GOGOI S, MANDAL S C, PATEL A B. Effect of dietaryWolffia arrhiza and Spirulina platensison growth performance and pigmentation of Queen loach Botia dario(Hamilton, 1822)[J]. Aquaculture Nutrition, 2018, 24(1): 285-291. |
[15] | PALMEGIANO G B, GAI F, DAPRÀ F, et al. Effects of Spirulina and plant oil on the growth and lipid traits of white sturgeon (Acipenser transmontanus) fingerlings[J]. Aquaculture Research, 2008, 39(6): 587-595. |
[16] | 曹申平, 韩冬, 解绶启, 等. 螺旋藻粉替代饲料中鱼粉对异育银鲫幼鱼生长、饲料利用和蛋白沉积的影响[J]. 水生生物学报, 2016, 40(4): 647-654. |
CAO S P, HAN D, XIE S Q, et al. Effects of dietary fishmeal replacement with Spirulina platensis powder on the growth performance, feed utilization and protein deposition in juvenile gibel carp(Carassis auratus gibelio var. cas)[J]. Acta Hydrobiologica Sinica, 2016, 40(4): 647-654.(in Chinese with English abstract) | |
[17] | ADEL M, YEGANEH S, DADAR M, et al. Effects of dietary Spirulina platensis on growth performance, humoral and mucosal immune responses and disease resistance in juvenile great sturgeon (Huso huso Linnaeus, 1754)[J]. Fish & Shellfish Immunology, 2016, 56: 436-444. |
[18] | YU W, WEN G L, LIN H Z, et al. Effects of dietary Spirulina platensis on growth performance, hematological and serum biochemical parameters, hepatic antioxidant status, immune responses and disease resistance of Coral trout Plectropomus leopardus(Lacepede, 1802)[J]. Fish & Shellfish Immunology, 2018, 74: 649-655. |
[19] | ROOHANI A M, ABEDIAN KENARI A, FALLAHI KAPOORCHALI M, et al. Effect of spirulina Spirulina platensisas a complementary ingredient to reduce dietary fish meal on the growth performance, whole-body composition, fatty acid and amino acid profiles, and pigmentation of Caspian brown trout (Salmo trutta caspius) juveniles[J]. Aquaculture Nutrition, 2019, 25(3): 633-645. |
[20] | TEIMOURI M, YEGANEH S, MIANJI G R, et al. The effect of Spirulina platensis meal on antioxidant gene expression, total antioxidant capacity, and lipid peroxidation of rainbow trout (Oncorhynchus mykiss)[J]. Fish Physiology and Biochemistry, 2019, 45(3): 977-986. |
[21] | MAHMOUD M M A, EL-LAMIE M M M, KILANY O E, et al. Spirulina(Arthrospira platensis) supplementation improves growth performance, feed utilization, immune response, and relieves oxidative stress in Nile tilapia (Oreochromis niloticus) challenged with Pseudomonas fluorescens[J]. Fish & Shellfish Immunology, 2018, 72: 291-300. |
[22] | LI H, LIU J G. Supplementation of Moina macrocopa with defatted Haematococcus pluvialis meal improved its growth performance and nutritional quality[J]. Aquaculture, 2021, 534: 736164. |
[23] | CAI M L, HUI W J, DENG X, et al. Dietary Haematococcus pluvialis promotes growth of red swamp crayfish Procambarus clarkii(Girard, 1852) via positive regulation of the gut microbial co-occurrence network[J]. Aquaculture, 2022, 551: 737900. |
[24] | 梁倩蓉, 郑天伦, 陈小明, 等. 饲料中添加蝇蛆蛋白对中华鳖肝和血清免疫代谢应答的影响[J]. 浙江农业学报, 2022, 34(10): 2172-2181. |
LIANG Q R, ZHENG T L, CHEN X M, et al. Effects of feeding with maggot protein added dietaries on immune and metabolic responses in liver and serum of soft-shelled turtles Pelodiscus sinensis[J]. Acta Agriculturae Zhejiangensis, 2022, 34(10): 2172-2181.(in Chinese with English abstract) | |
[25] | 武萌萌, 刘士伟, 李博生. 螺旋藻小分子多肽制备工艺的研究[J]. 浙江农业学报, 2010, 22(6): 802-807. |
WU M M, LIU S W, LI B S. Preparation and purification of low-molecular-weight peptides from Spirulina protein[J]. Acta Agriculturae Zhejiangensis, 2010, 22(6): 802-807.(in Chinese with English abstract) | |
[26] | JIANG M, ZHAO H H, ZAI S W, et al. A defatted microalgae meal (Haematococcus pluvialis) as a partial protein source to replace fishmeal for feeding juvenile yellow perch Perca flavescens[J]. Journal of Applied Phycology, 2019, 31(2): 1197-1205. |
[27] | JOSHUA W J, ZULPERI Z. Effects of Spirulina platensis and Chlorella vulgaris on the immune system and reproduction of fish[J]. Pertanika Journal of Tropical Agricultural Science, 2020, 43(4): 429-444. |
[28] | YU W J, ZHANG M J, WANG B J, et al. Dietary Haematococcus pluvialis powder supplementation affect carotenoid content, astaxanthin isomer, antioxidant capacity and immune-related gene expression in Pacific white shrimp, Litopenaeus vannamei[J]. Aquaculture Research, 2021, 52(6): 2403-2414. |
[29] | PHAM T H, CHENG T C, WANG P C, et al. Protective efficacy of four heat-shock proteins as recombinant vaccines against photobacteriosis in Asian seabass (Lates calcarifer)[J]. Fish & Shellfish Immunology, 2021, 111: 179-188. |
[30] | LEE D C, CHOI Y J, KIM J H. Toxic effects of waterborne cadmium exposure on hematological parameters, oxidative stress, neurotoxicity, and heat shock protein 70 in juvenile olive flounder, Paralichthys olivaceus[J]. Fish & Shellfish Immunology, 2022, 122: 476-483. |
[1] | LI Juncheng, DANG Yunzhi, SUN Qingming. Transcriptome analysis and HSP response of pitaya (Selenicereus spp.) under heat stress [J]. Acta Agriculturae Zhejiangensis, 2024, 36(5): 1067-1075. |
[2] | SONG Peng, LI Lixiang, JIANG Houlong, WANG Ru, LI Hui, ZHAO Pengyu, ZHANG Jun, QIN Pingwei, REN Jiangbo, CHEN Qingming. Effect of application of Brevibacillus laterosporus on potassium content of cured tobacco leaves and physiological characteristics of tobacco plants [J]. Acta Agriculturae Zhejiangensis, 2024, 36(3): 494-502. |
[3] | XIA Lunbin, MA Longlong, QIAO Deliang, HE Yanfei, JIANG Ping. Effects of Hyriopsis cumingii polysaccharides on growth performance, antioxidant activity and immune function in growing broiler [J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 547-555. |
[4] | LI Hongyi, ZHOU Runsheng, LIANG Xiaoling, ZHANG Chuyue, LYU Qixin, YANG Changhua, ZHANG Mao. Effect of dietary calcium and phosphorus level on Magang geese growth performance and liver gene expression [J]. Acta Agriculturae Zhejiangensis, 2023, 35(11): 2533-2542. |
[5] | LIN Xianyu, LI Ziqian, BAI Song, LUO Jun, QU Yan. Changes of antioxidant enzyme activity and differential expression of key genes in Camellia reticulata during drought-rehydration process [J]. Acta Agriculturae Zhejiangensis, 2023, 35(11): 2611-2620. |
[6] | GENG Bingjie, YE Miaomiao, CHEN Yan, WANG Mengchang, MA Shangyu, HUANG Zhenglai, ZHANG Wenjing, FAN Yonghui. Effects of exogenous 6-BA and KH2PO4 on antioxidant enzymes and anaerobic respiratory enzymes activities in wheat roots under waterlogging at post-flowering stage [J]. Acta Agriculturae Zhejiangensis, 2023, 35(10): 2275-2285. |
[7] | FAN Liying, FAN Tingting, TONG Zongjun, LIANG Liyun, ZHAO Zhiyong, CHEN Hui, ZHOU Changyan, ZHAO Xiaoyan. Effects on accumulation of cadmium and antioxidant system of different Morchella spp. under cadmium stress [J]. Acta Agriculturae Zhejiangensis, 2023, 35(10): 2321-2331. |
[8] | FANG Mingya, YU Hongwei, WU Yaxian, HAN Wenyan, LI Xin, LIU Haihe. Effects of exogenous epigallocatechin gallate on resistance of melon seedlings to powdery mildew [J]. Acta Agriculturae Zhejiangensis, 2023, 35(1): 138-145. |
[9] | HE Xiuli, WANG Renmin. Effects of exogenous melatonin on active components content and antioxidase activity of Anoectochilus roxburghii [J]. Acta Agriculturae Zhejiangensis, 2023, 35(1): 58-66. |
[10] | YAN Mei, YAO Yandong, MOU Kaiping, DAN Yuanyuan, LI Weitai, LIAO Weibiao. Involvement of abscisic acid in hydrogen gas-enhanced drought resistance by improving antioxidant enzyme activity and gene expression in tomato seedlings [J]. Acta Agriculturae Zhejiangensis, 2022, 34(9): 1901-1910. |
[11] | DING Dongxia, LI Nenghui, LI Jing, TANG Chaonan, WANG Cheng, NIU Tianhang, YANG Yan, YANG Haitao, XIE Jianming. Effects of exogenous melatonin on chlorophyll fluorescence and antioxidant system of pepper (Capsicum annuum L.) under low temperature and low light stress [J]. Acta Agriculturae Zhejiangensis, 2022, 34(9): 1935-1944. |
[12] | LI Yuting, LI Sha, CAO Jie, LI Jiaoyang, ZHANG Liang, XU Xiaofeng. Effects of microplastics on growth and antioxidant system of ectomycorrhizal fungi [J]. Acta Agriculturae Zhejiangensis, 2022, 34(5): 1049-1060. |
[13] | AN Yawen, YANG Xiaodong, GAO Zhixiong, GUO Shaoqian, GAO Aiwu, YANG Jinli, WANG Hairong. Effects of adding Sophora alopecuroides to high grain diet on growth and serum biochemical indexes of lambs [J]. Acta Agriculturae Zhejiangensis, 2022, 34(5): 908-914. |
[14] | WU Tao, WEI Yuming, JIANG Xiaofan, HUANG Jie, YANG Farong, CHEN Guoshun, CAI Yuan, JIAO Ting, ZHAO Shenguo. Effects of Chenopodium quinoa in diets on growth performance, slaughter performance, organ index and intestinal morphology of Luhua chickens [J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 255-265. |
[15] | CAI Yilong, ZHANG Libing, HU Gaoyu, XIAO Guoqiang, CAI Jingbo, ZHANG Xiang. Effects of different diets on growth performance and environmental water quality of Scylla paramamosain cultured in carb apartment [J]. Acta Agriculturae Zhejiangensis, 2022, 34(11): 2404-2415. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||