Effects of Spirulina platensis and Haematococcus pluvialis on the growth performance, antioxidant enzymes, phosphatase, and heat shock protein of zebrafish (Danio rerio)

doi:10.3969/j.issn.1004-1524.20231326

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (7): 1511-1518.DOI: 10.3969/j.issn.1004-1524.20231326

• Animal Science • Previous Articles Next Articles

Effects of Spirulina platensis and Haematococcus pluvialis on the growth performance, antioxidant enzymes, phosphatase, and heat shock protein of zebrafish (Danio rerio)

LI Fei¹(), SU Tiantian², SU Kangjie², XU Ke², MA Li², LIU Ziming²^,^*()

1. College of Ocean Engineering, Tangshan Maritime Institute, Tangshan 063200, Hebei, China
2. College of Ecology, Lishui University, Lishui 323000, Zhejiang, China

Received:2023-11-24 Online:2024-07-25 Published:2024-08-05

Abstract

Abstract:

Spirulina platensis and Haematococcus pluvialis are both high-quality microalgae with good nutritional and medicinal value. This study investigated the effects of dietary supplementation of 2% or 4% S. platensis and H. pluvialis on the growth performance, antioxidant enzyme and phosphatase activities, and heat shock protein expression of zebrafish (Danio rerio), which laid the foundation for the joint application of the two algae. The results showed that the final weight, weight gain and specific growth rates of zebrafish in group with 4% S. platensis and 2% H. pluvialis added to feed were significantly higher than those in the control group, and the gains were accompanied by significant increases in amylase and protease activities. The effects of dietary supplementation of two algae on the antioxidant enzyme activities of zebrafish were relatively complex. Compared with the control group, superoxide dismutase activities in two groups (2% S. platensis + 2% H. pluvialis and 4% S. platensis + 2% H. pluvialis) were significantly elevated. The opposite results were observed in catalase activities of zebrafish from two groups (4% S. platensis + 2% H. pluvialis and 4% S. platensis + 4% H. pluvialis). Zebrafish fed a diet supplemented with S. platensis and H. pluvialis had significant elevations in phosphatase activities and the expression of heat shock proteins. Phosphatase activities and heat shock protein 70 expression of zebrafish in all groups with algae supplementation were significantly higher than those in the control group, which illustrated that the combined feeding of S. platensis and H. pluvialis was conducive to improve the immune related levels and stress resistance of zebrafish. Compared with the control group, the significant elevation in the expression of heat shock protein 90 in zebrafish was detected in only one group (4% S. platensis + 2% H. pluvialis) rather than the other three groups. To sum up, dietary supplementation of S. platensis and H. pluvialis improved the growth performance, immune related level and anti-stress ability of zebrafish, and the addition with 4% S. platensis + 2% H. pluvialis was more appropriate.

Key words: Spirulina platensis, Haematococcus pluvialis, zebrafish, growth performance, antioxidant enzyme, phosp hatase, heat shock protein

CLC Number:

S963

LI Fei, SU Tiantian, SU Kangjie, XU Ke, MA Li, LIU Ziming. Effects of Spirulina platensis and Haematococcus pluvialis on the growth performance, antioxidant enzymes, phosphatase, and heat shock protein of zebrafish (Danio rerio)[J]. Acta Agriculturae Zhejiangensis, 2024, 36(7): 1511-1518.

Figures/Tables 7

References 30

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项目Item		对照组CK	SP2-HP2	SP2-HP4	SP4-HP2	SP4-HP4
成分	鱼粉Fish meal	50	46	44	44	42
Component/(g·kg^-1)	螺旋藻Spirulina	0	2	2	4	4
	雨生红球藻Haematococcus pluvialis	0	2	4	2	4
	小麦粉Wheat flour	16	16	16	16	16
	豆粉Soybean flour	16	16	16	16	16
	玉米淀粉Corn starch	10	10	10	10	10
	鱼油Fish oil	3	3	3	3	3
	豆油Soybean oil	3	3	3	3	3
	多维预混物Multivitamin premix ¹	1	1	1	1	1
	多矿预混物Multimineral premix ²	1	1	1	1	1
生化组成	粗蛋白Crude protein	45.54	45.50	45.43	45.31	45.09
Biochemical composition/%	粗脂肪Crude fat	9.08	9.15	9.21	9.24	9.28
	灰分Ash	7.65	7.67	7.73	7.74	7.69
	水分Water	8.64	8.58	8.67	8.55	8.60

项目Item		对照组CK	SP2-HP2	SP2-HP4	SP4-HP2	SP4-HP4
成分	鱼粉Fish meal	50	46	44	44	42
Component/(g·kg^-1)	螺旋藻Spirulina	0	2	2	4	4
	雨生红球藻Haematococcus pluvialis	0	2	4	2	4
	小麦粉Wheat flour	16	16	16	16	16
	豆粉Soybean flour	16	16	16	16	16
	玉米淀粉Corn starch	10	10	10	10	10
	鱼油Fish oil	3	3	3	3	3
	豆油Soybean oil	3	3	3	3	3
	多维预混物Multivitamin premix ¹	1	1	1	1	1
	多矿预混物Multimineral premix ²	1	1	1	1	1
生化组成	粗蛋白Crude protein	45.54	45.50	45.43	45.31	45.09
Biochemical composition/%	粗脂肪Crude fat	9.08	9.15	9.21	9.24	9.28
	灰分Ash	7.65	7.67	7.73	7.74	7.69
	水分Water	8.64	8.58	8.67	8.55	8.60

基因 Gene	引物序列 Primer sequence(5'-3')	GenBank编录号 GenBank accession No.	产物长度 Product length/bp
HSP70	F: CATGAAGAGCAGTGTGGAAGAC	AF210640	182
	R: TGGAGATGACTGGATTGCAG
HSP90	F: CCCATTGTGGAGACCCTCAGA	AF068773	179
	R: TCATCGATGCCAAGTCCGAG
β-actin	F: CATGGCTTCTGCTCTGTATG	AF026063	143
	R: GCAAAGTGGTAAACGCTTCT

基因 Gene	引物序列 Primer sequence(5'-3')	GenBank编录号 GenBank accession No.	产物长度 Product length/bp
HSP70	F: CATGAAGAGCAGTGTGGAAGAC	AF210640	182
	R: TGGAGATGACTGGATTGCAG
HSP90	F: CCCATTGTGGAGACCCTCAGA	AF068773	179
	R: TCATCGATGCCAAGTCCGAG
β-actin	F: CATGGCTTCTGCTCTGTATG	AF026063	143
	R: GCAAAGTGGTAAACGCTTCT

处理 Treatment	初始体重 Initial weight/g	终末体重 Terminal weight/g	体重增重率 Weight gain rate/%	特定生长率 Specific growth rate/(%·d^-1)	成活率 Survival rate/%
CK	0.025 5±0.001 2 a	0.061 1±0.003 1 a	139.5±10.2 a	1.455±0.072 a	96.27±2.89 a
SP2-HP2	0.025 1±0.000 8 a	0.072 1±0.010 5 ab	186.8±36.9 ab	1.747±0.215 bc	98.33±2.89 a
SP2-HP4	0.026 1±0.001 0 a	0.066 8±0.006 7 ab	156.0±17.3 a	1.564±0.113 ab	98.33±2.89 a
SP4-HP2	0.026 0±0.000 4 a	0.079 5±0.007 2 b	205.7±22.4 b	1.859±0.121 c	100.00±0.00 a
SP4-HP4	0.025 4±0.000 5 a	0.071 0±0.009 9 ab	179.8±37.2 ab	1.705±0.214 abc	98.33±2.89 a

Effects of Spirulina platensis and Haematococcus pluvialis on the growth performance, antioxidant enzymes, phosphatase, and heat shock protein of zebrafish (Danio rerio)

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