Effects of exogenous 6-BA and KH2PO4 on antioxidant enzymes and anaerobic respiratory enzymes activities in wheat roots under waterlogging at post-flowering stage

doi:10.3969/j.issn.1004-1524.20221487

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (10): 2275-2285.DOI: 10.3969/j.issn.1004-1524.20221487

• Crop Science • Previous Articles Next Articles

Effects of exogenous 6-BA and KH₂PO₄ on antioxidant enzymes and anaerobic respiratory enzymes activities in wheat roots under waterlogging at post-flowering stage

GENG Bingjie¹(), YE Miaomiao¹, CHEN Yan¹, WANG Mengchang¹, MA Shangyu¹^,²^,^*(), HUANG Zhenglai¹^,², ZHANG Wenjing¹, FAN Yonghui¹

1. Key Laboratory of Wheat Biology and Genetic Breeding in Southern Huanghuai, Ministry of Agriculture and Rural Affairs, College of Agronomy, Anhui Agricultural University, Hefei 230036, China
2. Collaborative Innovation Center of Modern Crop Production, Jiangsu Province, Nanjing 210095, China

Received:2022-10-25 Online:2023-10-25 Published:2023-10-31

Abstract

Abstract:

To investigate the effects of different exogenous substances on the growth and development of root system in waterlogged wheat plants, the cultivar Yangmai 18 was used as the experimental material. The plants were subjected to either waterlogging treatment for 9 days (W9) or normal irrigation (W0) during the flowering stage. Different exogenous substances were sprayed on the foliage, including distilled water (T0), 0.01 mmol·L^-1 6-BA (T1), 0.3% KH₂PO₄ (T2), and a mixture of 0.01 mmol·L^-1 6-BA and 0.3% KH₂PO₄ (T3). The effects of these exogenous substances on the activities of antioxidant enzymes and anaerobic respiratory enzymes in wheat root system were studied. The results showed that under W0 conditions, foliar application of 0.3% KH₂PO₄ and 0.01 mmol·L^-1 6-BA could improve the activities of superoxide dismutase (SOD), peroxidase (POD), and alcohol dehydrogenase (ADH) in the root system, increase the 1 000-grain weight of wheat, and ultimately enhance the yield. Under W9 treatment, the activities of SOD, ADH, and lactate dehydrogenase (LDH) in the wheat root system in the 0-60 cm soil layer were significantly reduced to varying degrees, while the activity of POD and the content of malondialdehyde (MDA) were significantly increased. However, the application of 0.3% KH₂PO₄ and 0.01 mmol·L^-1 6-BA significantly increased the activities of SOD, ADH, and LDH in the root system in each soil layer, reduced the activity of POD and the content of MDA. In particular, the combination of 0.01 mmol·L^-1 6-BA and 0.3% KH₂PO₄ significantly improved the antioxidant capacity and anaerobic respiration capacity of the wheat root system, resulting in 23.34% and 37.43% increase in 1 000-grain weight and yield compared to the waterlogged treatment.

Key words: wheat, root, waterlogging, antioxidant enzyme, anaerobic respiratory enzyme

CLC Number:

S512.1

GENG Bingjie, YE Miaomiao, CHEN Yan, WANG Mengchang, MA Shangyu, HUANG Zhenglai, ZHANG Wenjing, FAN Yonghui. Effects of exogenous 6-BA and KH₂PO₄ on antioxidant enzymes and anaerobic respiratory enzymes activities in wheat roots under waterlogging at post-flowering stage[J]. Acta Agriculturae Zhejiangensis, 2023, 35(10): 2275-2285.

Figures/Tables 8

Fig.1 Monthly precipitation and temperature at the experiment site from November 2020 to May 2021 November and December were in 2020; January, February, March, April and May were in 2021.

Fig.2 SOD activity of wheat roots under different treatments Data was detected based on fresh weight. Data marked without the same lowercase letter indicated significant differences at P<0.05. The same as below.

Fig.3 MDA content of wheat roots under different treatments

Fig.4 POD activity of wheat roots under different treatments

Fig.5 PDC activity of wheat roots under different treatments

Fig.6 LDH activity of wheat roots under different treatments

Fig.7 ADH activity of wheat roots under different treatments

Table 1 Yield and yield components of wheat under different treatments

处理	每管穗数	穗粒数	千粒重	每管产量	产量修复率
Treatment	Ear number per pipe	Grains per spike	1 000-grain weight/g	Yield per pipe/g	Yield recovery rate/%
W0T0	9.55 a	47.54 a	35.84 c	13.51 b	—
W0T1	9.61 a	49.09 a	41.86 b	16.39 a	—
W0T2	9.50 a	47.70 a	42.92 ab	16.14 a	—
W0T3	9.25 a	48.71 a	44.68 a	16.71 a	—
W9T0	9.75 a	44.72 a	27.94 c	9.27 c	—
W9T1	9.50 a	43.29 a	33.31 b	11.70 b	26.21
W9T2	9.53 a	44.68 a	32.62 b	11.89 b	28.26
W9T3	9.25 a	45.30 a	34.46 a	12.74 a	37.43

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Effects of exogenous 6-BA and KH₂PO₄ on antioxidant enzymes and anaerobic respiratory enzymes activities in wheat roots under waterlogging at post-flowering stage

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