Effects of exogenous 6-BA and KH2PO4 on antioxidant enzymes and anaerobic respiratory enzymes activities in wheat roots under waterlogging at post-flowering stage

doi:10.3969/j.issn.1004-1524.20221487

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (10): 2275-2285.DOI: 10.3969/j.issn.1004-1524.20221487

• Crop Science • Previous Articles Next Articles

Effects of exogenous 6-BA and KH₂PO₄ on antioxidant enzymes and anaerobic respiratory enzymes activities in wheat roots under waterlogging at post-flowering stage

GENG Bingjie¹(), YE Miaomiao¹, CHEN Yan¹, WANG Mengchang¹, MA Shangyu¹^,²^,^*(), HUANG Zhenglai¹^,², ZHANG Wenjing¹, FAN Yonghui¹

1. Key Laboratory of Wheat Biology and Genetic Breeding in Southern Huanghuai, Ministry of Agriculture and Rural Affairs, College of Agronomy, Anhui Agricultural University, Hefei 230036, China
2. Collaborative Innovation Center of Modern Crop Production, Jiangsu Province, Nanjing 210095, China

Received:2022-10-25 Online:2023-10-25 Published:2023-10-31

Abstract

Abstract:

To investigate the effects of different exogenous substances on the growth and development of root system in waterlogged wheat plants, the cultivar Yangmai 18 was used as the experimental material. The plants were subjected to either waterlogging treatment for 9 days (W9) or normal irrigation (W0) during the flowering stage. Different exogenous substances were sprayed on the foliage, including distilled water (T0), 0.01 mmol·L^-1 6-BA (T1), 0.3% KH₂PO₄ (T2), and a mixture of 0.01 mmol·L^-1 6-BA and 0.3% KH₂PO₄ (T3). The effects of these exogenous substances on the activities of antioxidant enzymes and anaerobic respiratory enzymes in wheat root system were studied. The results showed that under W0 conditions, foliar application of 0.3% KH₂PO₄ and 0.01 mmol·L^-1 6-BA could improve the activities of superoxide dismutase (SOD), peroxidase (POD), and alcohol dehydrogenase (ADH) in the root system, increase the 1 000-grain weight of wheat, and ultimately enhance the yield. Under W9 treatment, the activities of SOD, ADH, and lactate dehydrogenase (LDH) in the wheat root system in the 0-60 cm soil layer were significantly reduced to varying degrees, while the activity of POD and the content of malondialdehyde (MDA) were significantly increased. However, the application of 0.3% KH₂PO₄ and 0.01 mmol·L^-1 6-BA significantly increased the activities of SOD, ADH, and LDH in the root system in each soil layer, reduced the activity of POD and the content of MDA. In particular, the combination of 0.01 mmol·L^-1 6-BA and 0.3% KH₂PO₄ significantly improved the antioxidant capacity and anaerobic respiration capacity of the wheat root system, resulting in 23.34% and 37.43% increase in 1 000-grain weight and yield compared to the waterlogged treatment.

Key words: wheat, root, waterlogging, antioxidant enzyme, anaerobic respiratory enzyme

CLC Number:

S512.1

GENG Bingjie, YE Miaomiao, CHEN Yan, WANG Mengchang, MA Shangyu, HUANG Zhenglai, ZHANG Wenjing, FAN Yonghui. Effects of exogenous 6-BA and KH₂PO₄ on antioxidant enzymes and anaerobic respiratory enzymes activities in wheat roots under waterlogging at post-flowering stage[J]. Acta Agriculturae Zhejiangensis, 2023, 35(10): 2275-2285.

Figures/Tables 8

Fig.1 Monthly precipitation and temperature at the experiment site from November 2020 to May 2021 November and December were in 2020; January, February, March, April and May were in 2021.

Fig.2 SOD activity of wheat roots under different treatments Data was detected based on fresh weight. Data marked without the same lowercase letter indicated significant differences at P<0.05. The same as below.

Fig.3 MDA content of wheat roots under different treatments

Fig.4 POD activity of wheat roots under different treatments

Fig.5 PDC activity of wheat roots under different treatments

Fig.6 LDH activity of wheat roots under different treatments

Fig.7 ADH activity of wheat roots under different treatments

Table 1 Yield and yield components of wheat under different treatments

处理	每管穗数	穗粒数	千粒重	每管产量	产量修复率
Treatment	Ear number per pipe	Grains per spike	1 000-grain weight/g	Yield per pipe/g	Yield recovery rate/%
W0T0	9.55 a	47.54 a	35.84 c	13.51 b	—
W0T1	9.61 a	49.09 a	41.86 b	16.39 a	—
W0T2	9.50 a	47.70 a	42.92 ab	16.14 a	—
W0T3	9.25 a	48.71 a	44.68 a	16.71 a	—
W9T0	9.75 a	44.72 a	27.94 c	9.27 c	—
W9T1	9.50 a	43.29 a	33.31 b	11.70 b	26.21
W9T2	9.53 a	44.68 a	32.62 b	11.89 b	28.26
W9T3	9.25 a	45.30 a	34.46 a	12.74 a	37.43

References 45

[1]	YANG H S, ZHAI S L, LI Y F, et al. Waterlogging reduction and wheat yield increase through long-term ditch-buried straw return in a rice-wheat rotation system[J]. Field Crops Research, 2017, 209: 189-197.
[2]	WANG X Y, LIU D Z, WEI M M, et al. Spraying 6-BA could alleviate the harmful impacts of waterlogging on dry matter accumulation and grain yield of wheat[J]. PeerJ, 2020.[2022-10-24.] https://peerj.com/articles/8193/.
[3]	SETTER T L, WATERS I. Review of prospects for germplasm improvement for waterlogging tolerance in wheat, barley and oats[J]. Plant and Soil, 2003, 253(1): 1-34.
[4]	石春林, 金之庆. 基于WCSODS的小麦渍害模型及其在灾害预警上的应用[J]. 应用气象学报, 2003, 14(4): 462-468.
	SHI C L, JIN Z Q. Wheat waterlogging damage model based on WCSODS and its application in disaster early warning[J]. Journal of Applied Meteorological Science, 2003, 14(4): 462-468. (in Chinese)
[5]	高敬文, 苏瑶, 沈阿林. 渍害胁迫下小麦生长的响应机理及调控措施研究进展[J]. 应用生态学报, 2020, 31(12): 4321-4330.
	GAO J W, SU Y, SHEN A L. Research progress of the response mechanism of wheat growth to waterlogging stress and the related regulating managements[J]. Chinese Journal of Applied Ecology, 2020, 31(12): 4321-4330. (in Chinese with English abstract)
[6]	李金才, 董琦, 余松烈. 不同生育期根际土壤淹水对小麦品种光合作用和产量的影响[J]. 作物学报, 2001, 27(4): 434-441.
	LI J C, DONG Q, YU S L. Effect of waterlogging at different growth stages on photosynthesis and yield of different wheat cultivars[J]. Acta Agronomica Sinica, 2001, 27(4): 434-441. (in Chinese with English abstract)
[7]	PAMPANA S, MASONI A, ARDUINI I. Grain yield of durum wheat as affected by waterlogging at tillering[J]. Cereal Research Communications, 2016, 44(4): 706-716.
[8]	ARDUINI I, ORLANDI C, PAMPANA S, et al. Waterlogging at tillering affects spike and spikelet formation in wheat[J]. Crop and Pasture Science, 2016, 67(7): 703.
[9]	SHARMA P K, SHARMA S K, CHOI I Y. Individual and combined effects of waterlogging and alkalinity on yield of wheat (Triticum aestivum L.) imposed at three critical stages[J]. Physiology and Molecular Biology of Plants: an International Journal of Functional Plant Biology, 2010, 16(3): 317-320.
[10]	赵辉, 荆奇, 戴廷波, 等. 花后高温和水分逆境对小麦籽粒蛋白质形成及其关键酶活性的影响[J]. 作物学报, 2007, 33(12): 2021-2027.
	ZHAO H, JING Q, DAI T B, et al. Effects of post-anthesis high temperature and water stress on activities of key regulatory enzymes involved in protein formation in two wheat cultivars[J]. Acta Agronomica Sinica, 2007, 33(12): 2021-2027. (in Chinese with English abstract)
[11]	林贤青, 沈惠聪, 季吟秋, 等. 油菜渍害若干生理问题的探讨[J]. 浙江农业大学学报, 1993, 19(1): 19-23.
	LIN X Q, SHEN H C, JI Y Q, et al. Research on some physiological effects of waterlogging in rape (B. napus)[J]. Journal of Zhejiang Agricultural University, 1993, 19(1): 19-23. (in Chinese with English abstract)
[12]	VOESENEK L A C J, SASIDHARAN R. Ethylene- and oxygen signalling-drive plant survival during flooding[J]. Plant Biology, 2013, 15(3): 426-435.
[13]	GAO J W, SU Y, YU M, et al. Potassium alleviates post-anthesis photosynthetic reductions in winter wheat caused by waterlogging at the stem elongation stage[J]. Frontiers in Plant Science, 2021, 11: 607475.
[14]	谢祝捷, 姜东, 曹卫星, 等. 花后干旱和渍水条件下生长调节物质对冬小麦光合特性和物质运转的影响[J]. 作物学报, 2004, 30(10): 1047-1052.
	XIE Z J, JIANG D, CAO W X, et al. Effects of plant growth regulation substances on photosynthetic characteristics and assimilates transportation in winter wheat under post-anthesis drought and waterlogging[J]. Acta Agronomica Sinica, 2004, 30(10): 1047-1052. (in Chinese with English abstract)
[15]	李金才, 魏凤珍, 余松烈, 等. 孕穗期渍水对冬小麦根系衰老的影响[J]. 应用生态学报, 2000, 11(5): 723-726.
	LI J C, WEI F Z, YU S L, et al. Effect of waterlogging on senescence of winter wheat root system at booting stage[J]. Chinese Journal of Applied Ecology, 2000, 11(5): 723-726. (in Chinese with English abstract)
[16]	李金才, 魏凤珍, 王成雨, 等. 孕穗期土壤渍水逆境对冬小麦根系衰老的影响[J]. 作物学报, 2006, 32(9): 1355-1360.
	LI J C, WEI F Z, WANG C Y, et al. Effects of waterlogging on senescence of root system at booting stage in winter wheat[J]. Acta Agronomica Sinica, 2006, 32(9): 1355-1360. (in Chinese with English abstract)
[17]	柳道明, 贾文婕, 王小燕, 等. 喷施外源6-BA对小麦孕穗期渍害的调控效应[J]. 作物杂志, 2015(2): 84-88.
	LIU D M, JIA W J, WANG X Y, et al. Effects of exogenous 6-BA on wheat with waterlogging at booting stage[J]. Crops, 2015(2): 84-88. (in Chinese with English abstract)
[18]	JIANG D, FAN X M, DAI T B, et al. Nitrogen fertiliser rate and post-anthesis waterlogging effects on carbohydrate and nitrogen dynamics in wheat[J]. Plant and Soil, 2008, 304(1/2): 301-314.
[19]	李金才, 魏凤珍, 余松烈, 等. 孕穗期湿害对小麦灌浆特性及产量的影响[J]. 安徽农业大学学报, 1999, 26(1): 89-94.
	LI J C, WEI F Z, YU S L, et al. Effects of waterlogging on filling characteristics and yield in wheat at booting stage[J]. Journal of Anhui Agricultural University, 1999, 26(1): 89-94. (in Chinese with English abstract)
[20]	徐莉, 陈小洁, 曹静婷, 等. 小麦赤霉病生防菌DZSG23的抗病机制[J]. 浙江农业学报, 2020, 32(11): 2001-2008.
	XU L, CHEN X J, CAO J T, et al. Resistance mechanism of biocontrol strain DZSG23 against wheat scab[J]. Acta Agriculturae Zhejiangensis, 2020, 32(11): 2001-2008. (in Chinese with English abstract)
[21]	张国平, Stanley M. 几种化学物质对小麦叶片衰老的延缓作用[J]. 浙江农业学报, 1994, 6(2): 94-97.
	ZHANG G P, STANLEY M. Some important biochemical properties in developing sweet potato:[J]. Acta Agriculturae Zhejiangensis, 1994, 6(2): 94-97. (in Chinese with English abstract)
[22]	李春喜, 尚玉磊, 姜丽娜, 等. 不同植物生长调节剂对小麦衰老及产量构成的调节效应[J]. 西北植物学报, 2001, 21(5): 931-936.
	LI C X, SHANG Y L, JIANG L N, et al. Regulation of plant growth regulator on leaves senescence and yield constitutions of wheat[J]. Acta Botanica Boreali-Occidentalia Sinica, 2001, 21(5): 931-936. (in Chinese with English abstract)
[23]	张嘉程, 吕绪方, 王稳江. 花后喷施叶面肥对小麦生理特性及产量的影响[J]. 贵州农业科学, 2021, 49(7): 26-32.
	ZHANG J C, LYU X F, WANG W J. Effects of foliar fertilizer after anthesis on physiological characteristics and yield of wheat[J]. Guizhou Agricultural Sciences, 2021, 49(7): 26-32. (in Chinese with English abstract)
[24]	吴国梁, 崔秀珍, 宋小顺, 等. 壤质土叶面喷施不同养分对强筋小麦产量和蛋白质组分的影响[J]. 吉林农业科学, 2006, 31(6): 19-21.
	WU G L, CUI X Z, SONG X S, et al. Effect of leaves spraying of different nutrients on the yield and protein component of strong glutinous wheat growing on loam soil[J]. Journal of Jilin Agricultural Sciences, 2006, 31(6): 19-21. (in Chinese with English abstract)
[25]	杜厚江, 王小燕, 赵晓宇. 6-BA对小麦开花期渍害的缓减效应[J]. 麦类作物学报, 2014, 34(12): 1672-1676.
	DU H J, WANG X Y, ZHAO X Y. Effects of 6-BA on alleviating grain yield loss of wheat by waterlogging at anthesis[J]. Journal of Triticeae Crops, 2014, 34(12): 1672-1676. (in Chinese with English abstract)
[26]	董金鑫. 孕穗期渍水对不同小麦品种根系生长和籽粒产量的影响[D]. 扬州: 扬州大学, 2022.
	DONG J X. Effects of waterlogging at booting stage on root growth and grain yield of different wheat varieties[D]. Yangzhou: Yangzhou University, 2022. (in Chinese with English abstract)
[27]	谢凯, 赵辰颖, 宋韵琳, 等. 渍前和渍后喷施外源调节物质对花期渍水小麦生长和产量减损效应的差异[J]. 麦类作物学报, 2023, 43(5): 623-631.
	XIE K, ZHAO C Y, SONG Y L, et al. Effects of spraying exogenous regulatory substances before and after waterlogging on growth and yield loss of waterlogged wheat at flowering stage[J]. Journal of Triticeae Crops, 2023, 43(5): 623-631. (in Chinese)
[28]	丁锦峰, 苏盛楠, 梁鹏, 等. 拔节期和花后渍水对小麦产量、干物质及氮素积累和转运的影响[J]. 麦类作物学报, 2017, 37(11): 1473-1479.
	DING J F, SU S N, LIANG P, et al. Effect of waterlogging at elongation or after anthesis on grain yield and accumulation and remobilization of dry matter and nitrogen in wheat[J]. Journal of Triticeae Crops, 2017, 37(11): 1473-1479. (in Chinese with English abstract)
[29]	赵晓宇, 王小燕, 柳道明, 等. 6-苄氨基腺嘌呤对江汉平原小麦开花后渍害的缓减效应研究[J]. 长江流域资源与环境, 2015, 24(3): 518-523.
	ZHAO X Y, WANG X Y, LIU D M, et al. Research on distributing characteristics of precipitation during grain filling in Jianghan plain and mitigation of waterlogging by 6-BA in wheat[J]. Resources and Environment in the Yangtze Basin, 2015, 24(3): 518-523. (in Chinese with English abstract)
[30]	马尚宇, 韩笑, 王艳艳, 等. 花后渍水对小麦根系形态、生理及地上部干物质分配和产量的影响[J]. 南京农业大学学报, 2021, 44(4): 614-621.
	MA S Y, HAN X, WANG Y Y, et al. Effect of waterlogging stress after anthesis on the root morphology, physiology, dry matter distribution and yield in wheat[J]. Journal of Nanjing Agricultural University, 2021, 44(4): 614-621. (in Chinese with English abstract)
[31]	王学奎, 黄见良. 植物生理生化实验原理与技术[M]. 3版. 北京: 高等教育出版社, 2015.
[32]	GHORBANI A. Role of salicylic acid pretreatment in alleviating cadmium-induced toxicity in Salvia officinalis L[J]. 2018.
[33]	AMADOR M L, SANCHO S, BIELSA B, et al. Physiological and biochemical parameters controlling waterlogging stress tolerance in Prunus before and after drainage[J]. Physiologia Plantarum, 2012, 144(4): 357-368.
[34]	吴进东, 李金才, 魏凤珍, 等. 氮肥和6-BA对花后受渍冬小麦抗渍性的调控效应[J]. 西北植物学报, 2012, 32(12): 2512-2517.
	WU J D, LI J C, WEI F Z, et al. Regulatory effects of nitrogen nutrient and 6-BA on winter wheat suffered post-anthesis waterlogging[J]. Acta Botanica Boreali-Occidentalia Sinica, 2012, 32(12): 2512-2517. (in Chinese with English abstract)
[35]	刘芬, 屈成, 王悦, 等. 抽穗期干旱复水对机插水稻抗氧化酶活性及根系活力的影响[J]. 南方农业学报, 2020, 51(1): 65-71.
	LIU F, QU C, WANG Y, et al. Effects of drought rewatering at heading stage on antioxidant enzyme activity and root activity of machine-transplanted rice[J]. Journal of Southern Agriculture, 2020, 51(1): 65-71. (in Chinese with English abstract)
[36]	TAN W, LIU J, DAI T, et al. Alterations in photosynthesis and antioxidant enzyme activity in winter wheat subjected to post-anthesis water-logging[J]. Photosynthetica, 2008, 46(1): 21-27.
[37]	马尚宇, 刘雅男, 王笑然, 等. 花后渍水时长对小麦根系形态和抗氧化酶活性及产量的影响[J]. 麦类作物学报, 2021, 41(4): 499-507.
	MA S Y, LIU Y N, WANG X R, et al. Effects of waterlogging duration after anthesis on root morphology, antioxidant enzyme activity and yield of wheat[J]. Journal of Triticeae Crops, 2021, 41(4): 499-507. (in Chinese with English abstract)
[38]	VODNIK D, STRAJNAR P, JEMC S, et al. Respiratory potential of maize (Zea mays L.) roots exposed to hypoxia[J]. Environmental and Experimental Botany, 2009, 65(1): 107-110.
[39]	杨笑彦, 朱建强. 6-苄氨基腺嘌呤与化肥配施减轻小麦灌浆期渍涝危害[J]. 江苏农业科学, 2016, 44(7): 129-131.
	YANG X Y, ZHU J Q. Combined application of 6-benzylamino adenine and chemical fertilizer to reduce waterlogging damage in wheat filling stage[J]. Jiangsu Agricultural Sciences, 2016, 44(7): 129-131. (in Chinese)
[40]	李颖, 赵继浩, 李金融, 等. 外源6-BA对不同生育时期淹水花生根系生长和荚果产量的影响[J]. 中国农业科学, 2020, 53(18): 3665-3678.
	LI Y, ZHAO J H, LI J R, et al. Effects of exogenous 6-BA on root growth and pod yield of flooded peanut at different growth stages[J]. Scientia Agricultura Sinica, 2020, 53(18): 3665-3678. (in Chinese with English abstract)
[41]	MA S Y, GAI P P, WANG Y Y, et al. Carbohydrate assimilation and translocation regulate grain yield formation in wheat crops (Triticum aestivum L.) under post-flowering waterlogging[J]. Agronomy, 2021, 11(11): 2209.
[42]	ARAKI H, HAMADA A, HOSSAIN M A, et al. Waterlogging at jointing and/or after anthesis in wheat induces early leaf senescence and impairs grain filling[J]. Field Crops Research, 2012, 137: 27-36.
[43]	DING J F, LIANG P, WU P, et al. Effects of waterlogging on grain yield and associated traits of historic wheat cultivars in the middle and lower reaches of the Yangtze River, China[J]. Field Crops Research, 2020, 246: 107695.
[44]	王三根. 细胞分裂素在植物抗逆和延衰中的作用[J]. 植物学通报, 2000, 35(2): 121-126, 167.
	WANG S G. Roles of cytokinin on stress-resistance and delaying senescence in plants[J]. Chinese Bulletin of Botany, 2000, 35(2): 121-126, 167. (in Chinese with English abstract)
[45]	钱兆国, 丛新军, 孙宪印, 等. 喷施磷酸二氢钾对小麦粒重的影响[J]. 安徽农业科学, 2001, 29(4): 511-513.
	QIAN Z G, CONG X J, SUN X Y, et al. Effect of spraying the solution of KH₂PO₄ on grain weight[J]. Journal of Anhui Agricultural Sciences, 2001, 29(4): 511-513. (in Chinese with English abstract)

Effects of exogenous 6-BA and KH₂PO₄ on antioxidant enzymes and anaerobic respiratory enzymes activities in wheat roots under waterlogging at post-flowering stage

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 45

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Jun, ZHANG Bo, HU Bibo, LIU Jingliang, ZHANG Xiaoyu, LI Chunyang, XIONG Shengting, GUO Binbin, WANG Xiucun, MA Chao. Identification and expression analysis of members of the SWEET and SUT families in wheat (Triticum aestivum L.) [J]. Acta Agriculturae Zhejiangensis, 2025, 37(9): 1825-1839.
[2]	WANG Xiaohui, JIA Sainan, FENG Jiayu, YIN Xinyue, LIU Zixuan, LIU Wenjie, ZHAO Shuaiying, WANG Shujing, TANG Yuehui. Cloning and function analysis of JcMBY27 gene from Jatropha curcas [J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1658-1665.
[3]	TAN Haixia, PENG Hongli, WANG Lianlong, WEI Jianmei. Differences in soil microbial community diversity between healthy and scab-diseased potato plants in root zone [J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1743-1754.
[4]	WANG Chengyang, LIU Jieya, WU Minyi, XIE Boyi, HONG Decheng, LENG Feng, WU Guoquan. Effect of calcium treatment on the fruit quality of Reliance grape under waterlogging [J]. Acta Agriculturae Zhejiangensis, 2025, 37(7): 1451-1458.
[5]	DENG Yulian, TAN Lin, NIU Li, LU Qiancheng, ZHOU Linghong, LI Guihua, HU Qiulong. Screening and identification of an antagonistic fungus strain against root rot disease in tea plants and its biological characteristics [J]. Acta Agriculturae Zhejiangensis, 2025, 37(7): 1469-1480.
[6]	WANG Yidi, WANG Jinglei, HU Tianhua, XU Yunmin, BAO Chonglai. Development of molecular markers for clubroot resistance and their application in Brassicaceae breeding [J]. Acta Agriculturae Zhejiangensis, 2025, 37(6): 1272-1284.
[7]	SHEN Lan, YANG Xiaofang, ZHANG Guofang. Identification and fungicide sensitivity of the pathogen causing root rot on strawberry [J]. Acta Agriculturae Zhejiangensis, 2025, 37(2): 417-425.
[8]	XIONG Tao, YAN Miao, WU Ting, MA Chao, YANG Juntao, HU Guozhi. Effects of potassium fulvic acid on soil microecology, root morphology in root zone of melon and fruit quality [J]. Acta Agriculturae Zhejiangensis, 2025, 37(10): 2066-2076.
[9]	LIU Shengnan, ZHU Jianyi, LI Ming, ZHAO Haoyu, XIONG Tao, TANG Yonglu, ZHOU Xiaogang, LI Chaosu. Weed control efficacy and wheat yield in no-tillage rotary sowing after rice stubble [J]. Acta Agriculturae Zhejiangensis, 2025, 37(10): 2129-2137.
[10]	HAN Xiao, LIU Xujie, SHI Lyu, ZHANG Jin, SHAN Haiyong, SHI Xiaoxu, YAN Yini, LIU Jian, XUE Yaguang. Effects of reduced application of controlled-release nitrogen fertilizer on rice yield, quality and nitrogen fertilizer utilization efficiency under concentrated coverage of wheat straw between rows for returning to field [J]. Acta Agriculturae Zhejiangensis, 2025, 37(1): 1-13.
[11]	YANG Xiaoyu, MA Zhihui, WEI Qing, NIU Zhipeng, CHEN Anqi, HU Zhengchong, WANG Linsheng. Preliminary mapping of a wheat awn length gene and prediction of candidate genes [J]. Acta Agriculturae Zhejiangensis, 2025, 37(1): 14-23.
[12]	MIN Jiangyan, TANG Zhuolei, YANG Xue, HUANG Xiaoyan, HUANG Kaifeng, HE Peiyun. Effect of different drought-rewatering modes on growth and yield of Tartary buckwheat [J]. Acta Agriculturae Zhejiangensis, 2024, 36(9): 2000-2009.
[13]	SHEN Zhengrong, DAI Yuanxing, GUO Liuming, WANG Zhiyao, ZHANG Hengmu. Preparation and application of specific antibody against coat protein (CP) of Chinese wheat mosaic virus (CWMV) [J]. Acta Agriculturae Zhejiangensis, 2024, 36(9): 2042-2050.
[14]	WANG Xiaoyang, LI Qiang, ZHAO Wuyun, DAI Fei, YAN Zhaorong, WANG Jiuxin. Design and experiment of shovel-type combined machine for silage corn root stubble plucking and residual plastic film collecting [J]. Acta Agriculturae Zhejiangensis, 2024, 36(9): 2132-2145.
[15]	LI Fei, SU Tiantian, SU Kangjie, XU Ke, MA Li, LIU Ziming. Effects of Spirulina platensis and Haematococcus pluvialis on the growth performance, antioxidant enzymes, phosphatase, and heat shock protein of zebrafish (Danio rerio) [J]. Acta Agriculturae Zhejiangensis, 2024, 36(7): 1511-1518.