Abstract: The genomic DNA of 24 main wheat cultivars in Huai'an Region were amplified by 86 SSR primers located on 42 chromosomes of wheat.The results showed that 26 out of 86 SSR primers could amplify stable polymorphism among 24 wheat cultivars. Genetic similarity of 24 wheat cultivars were analyzed by 124 alleles detected from 26 pairs of primers. Six out of 26 pairs of polymorphic SSR primers were selected for their clear bands and high polymorphism to construct the fingerprinting maps of the 24 wheat cultivars. All the 24 wheat cultivars could be distinguished from each other by the fingerprint map constituted by 6 pairs of SSR primers. The genetic similarity coefficients of the 24 wheat cultivars ranged from 054 to 092. Furthermore, all the cultivars were classified into 4 groups, at the level of genetic similarity coefficient 064 according to cluster analysis by an un-weighted pair-group average method with arithmetic mean, which revealed the genetic relationship of cultivars to a degree．

Key words: wheat, SSR markers, fingerprinting map, genetic similarity