醋酸钠及复配4种矿物质对草菇菌丝生物量的影响

doi:10.3969/j.issn.1004-1524.2018.02.07

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (2): 228-235.DOI: 10.3969/j.issn.1004-1524.2018.02.07

醋酸钠及复配4种矿物质对草菇菌丝生物量的影响

侯立娟¹, 林金盛¹, 刘少华², 李瑞祥², 马林¹, 蒋宁¹, 曲绍轩¹, 李辉平¹

1.江苏省农业科学院蔬菜研究所,江苏省高效园艺作物遗传改良重点实验室,江苏南京 210014;
2.鲁东大学农学院,山东烟台 264025

收稿日期:2017-05-25 出版日期:2018-02-20 发布日期:2018-02-11
作者简介:侯立娟(1981—),女,吉林松原人,博士,副研究员,主要从事菌类作物栽培生理及菌糠的综合利用研究。E-mail:mybailinggu@126.com
基金资助:
江苏省自然青年基金(BK20140742); 江苏省农业科技自主创新项目[CX(13)5012]; 江苏省双创博士基金

Sodium acetate and compound with four kinds of minerals on the mycelium biomass of Volvariella volvacea

HOU Lijuan¹, LIN Jinsheng¹, LIU Shaohua², LI Ruixiang², MA Lin¹, JIANG Ning¹, QU Shaoxuan¹, LI Huiping¹

1.Institute of Vegetable Crop, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China;
2. School of Agriculture, Ludong University, Yantai 264025, China

Received:2017-05-25 Online:2018-02-20 Published:2018-02-11

摘要/Abstract

摘要： 通过测定菌丝DNA含量,检测不同浓度梯度醋酸钠对草菇菌丝生物量的影响,结果表明,随着醋酸钠浓度的升高,菌丝生物量呈线性增加,当达到0.7 g·L^-1时,生物量达到一个高峰值,DNA的含量为468.9±4.10 μg·g^-1,极显著地高于对照组,随着醋酸钠浓度继续增加到0.9 g·L^-1和1.1 g·L^-1时,生物量则呈减少的趋势,但均比对照的生物量高,说明适宜的醋酸钠浓度能极显著地增加草菇菌丝生物量。在筛选出最佳醋酸钠浓度(0.7 g·L^-1)的基础上,进一步复配Cu、Zn、K、Mn元素,以醋酸钠为对照,比较复配微量元素及同一微量元素不同浓度对草菇菌丝生物量的影响。结果显示,添加矿物质K和Cu元素效果较好,各浓度均能使菌丝生物量有所增加,其中添加8 g·L^-1 KH₂PO₄处理的菌丝生物量比对照的高84.32%(P<0.05);添加0.04 g·L^-1 CuSO₄处理的菌丝生物量比对照高73.19%,达到5%显著水平;ZnSO₄添加量为0.25 g·L^-1和0.20 g·L^-1两处理的菌丝生物量分别比对照提高42.69%和20.34%;添加0.35 g·L^-1 MnSO₄处理的菌丝生物量比对照提高25.23%以上。Zn和Mn元素的各浓度处理与对照的菌丝体生物量无显著差异。

关键词: 醋酸钠, 草菇, 生物量, 菌丝, 矿质元素

Abstract: In this paper, mycelial dry weight of mycelium and DNA content of Volvariella volvacea were determined to detect the effect of different concentrations of sodium acetate on mycelial biomass of V. volvacea. The results showed that with the increase of concentration of sodium acetate, mycelial biomass increased linearly. When the concentration reached 0.7 g·L^-1, the biomass reached the peak, and the content of DNA was 468.9 ±4.10 μg·g^-1, which was significantly higher than that of CK. While the concentration of sodium acetate was 0.9 g·L^-1 and 1.1 g·L^-1, the biomass decreased, but higher than that of CK. It was concluded that the suitable concentration of sodium acetate can significantly increase the mycelium dry weight of V. volvacea. Based on the optimal concentration of sodium acetate (0.7 g·L^-1), the effect of Cu, Zn, K, Mn element and compound trace element on mycelia biomass were compared. The results showed that K and Cu element showed good effect, the mycelial biomass under all concentrations increased. Compared with CK, when the application amount of KH₂PO₄ was 8 g·L^-1, the mycelial biomass was significantly increased by 84.32% (P<0.05); when the application amount of CuSO₄ was 0.04 g·L^-1, the mycelia biomass was significantly increased by 73.19% (P<0.05); when the application amount of ZnSO₄ were 0.25 g·L^-1 and 0.20 g·L^-1, the mycelia biomass was increased by 42.69% and 20.34%, respectively; when the application amount of MnSO₄ was 0.35 g·L^-1, the mycelia biomass was increased by 25.23%. The Zn element and Mn element showed no significant effect on mycelium biomass with CK.

Key words: sodium acetate, Volvariella volvacea, biomass, hyphae, mineral elements

中图分类号:

S646.1⁺3

侯立娟, 林金盛, 刘少华, 李瑞祥, 马林, 蒋宁, 曲绍轩, 李辉平. 醋酸钠及复配4种矿物质对草菇菌丝生物量的影响[J]. 浙江农业学报, 2018, 30(2): 228-235.

HOU Lijuan, LIN Jinsheng, LIU Shaohua, LI Ruixiang, MA Lin, JIANG Ning, QU Shaoxuan, LI Huiping. Sodium acetate and compound with four kinds of minerals on the mycelium biomass of Volvariella volvacea[J]. , 2018, 30(2): 228-235.

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醋酸钠及复配4种矿物质对草菇菌丝生物量的影响

Sodium acetate and compound with four kinds of minerals on the mycelium biomass of Volvariella volvacea

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