隐绿原酸对H9N2-AIV致小鼠肺损伤的免疫调节和抗氧化效果

doi:10.3969/j.issn.1004-1524.2022.01.05

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (1): 33-40.DOI: 10.3969/j.issn.1004-1524.2022.01.05

隐绿原酸对H9N2-AIV致小鼠肺损伤的免疫调节和抗氧化效果

张昕¹(), 张铭¹, 何宏轩²^,^*(), 胡延春¹^,^*()

1.四川农业大学动物医学院,四川成都 611130
2.中国科学院动物研究所,北京 100101

收稿日期:2020-09-14 出版日期:2022-01-25 发布日期:2022-02-05
作者简介:何宏轩,E-mail: hehx@ioz.ac.cn
* 胡延春,E-mail: hychun114@163.com;
张昕(1996—),女,四川巴中人,硕士研究生,研究方向为动物疾病诊疗。E-mail: 903391909@qq.com
通讯作者: 何宏轩,胡延春
基金资助:
北京市家禽产业创新团队(BAIC04-2020)

Effect of cryptochlorogenic acid on immunomodulatory and antioxidant in mice of lung injury induced by H9N2-AIV

ZHANG Xin¹(), ZHANG Ming¹, HE Hongxuan²^,^*(), HU Yanchun¹^,^*()

1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
2. Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Received:2020-09-14 Online:2022-01-25 Published:2022-02-05
Contact: HE Hongxuan,HU Yanchun

摘要/Abstract

摘要：

为探讨隐绿原酸(4-CQA)对感染H9N2亚型禽流感(H9N2-AIV)病毒的小鼠造成肺损伤的修复作用,将90只6~8周龄健康昆明系小鼠随机分为病毒(H9N2)感染组、H9N2+4-CQA组、空白对照组、4-CQA对照组,用H9N2-AIV滴鼻复制小鼠肺损伤模型并灌胃4-CQA干预。观察各组小鼠肺组织病理学变化;测定肺指数及肺内丙二醛(MDA)、总超氧化物歧化酶(T-SOD)、髓过氧化物酶(MPO)含量;测定脾内T淋巴细胞亚群CD4⁺、CD8⁺百分比及比率(CD4⁺/CD8⁺)。结果显示,H9N2组小鼠表现出精神沉郁、呼吸急促;肺指数上升、脾指数下降;肺组织病理学表现为肺泡萎缩、肺泡隔增厚、局部肺实变;T淋巴细胞亚群CD4⁺/CD8⁺比率显著下降(P<0.05);肺内T-SOD活性显著下降、MDA含量显著增加、MPO活性显著升高(P<0.05)。4-CQA干预后,小鼠肺指数下降、脾指数上升;肺组织病理学损伤减轻;T淋巴细胞亚群CD4⁺/CD8⁺比率显著上升(P<0.05);肺内T-SOD活性显著升高,MDA含量显著降低,MPO活性显著下降(P<0.05)。得出4-CQA可通过维持机体免疫平衡、降低机体氧化损伤来缓解由H9N2亚型AIV诱导的小鼠肺损伤。

关键词: H9N2亚型禽流感病毒, 隐绿原酸, 肺损伤, 免疫调节, 抗氧化

Abstract:

To investigate the effect of cryptochlorogenic acid(4-CQA)on lung injury and recovery in mice infected with avian influenza H9N2 virus, ninety SPF KM mice(6-8 weeks old)were randomly divided into H9N2 group,H9N2+4-CQA group, control group and 4-CQA group. Then H9N2-AIV was used to induce lung injury and received 4-CQA intragastric administration respectively in mice. The lung histopathlogy changes, the lung index and spleen index, and the contents of malondialdehyde (MDA),total superoxide dismutase (T-SOD) and myeloperoxidase (MPO) in the lung tissue homogenate, and the ratio of T cell subgroups (CD4⁺、CD8⁺、CD4⁺/CD8⁺) were observed. The results showed that the group of H9N2 presented depression and shortness of breath; lung index increased and spleen index decreased; histopathological manifestations of lung were alveolar atrophy, alveolar septal thickening and local lung consolidation; CD4⁺/CD8⁺ ratio of T lymphocyte subsets decreased significantly (P<0.05), activity of T-SOD decreased significantly, content of MDA increased significantly, and activity of MPO increased significantly (P<0.05). After 4-CQA treatment, lung index decreased and spleen index increased; lung histopathological damage was alleviated; CD4⁺/CD8⁺ ratio of T cell subgroups was significantly increased (P<0.05); T-SOD and MPO activity in lung were significantly increased and the content of MDA was significantly decreased (P<0.05). It was concluded that 4-CQA can alleviate the lung injury induced by avian influenza H9N2 virus by maintaining immune balance and reducing oxidative damage.

Key words: H9N2-AIV, 4-CQA, lung injury, immunomodulation, antioxidant

中图分类号:

S855.3

张昕, 张铭, 何宏轩, 胡延春. 隐绿原酸对H9N2-AIV致小鼠肺损伤的免疫调节和抗氧化效果[J]. 浙江农业学报, 2022, 34(1): 33-40.

ZHANG Xin, ZHANG Ming, HE Hongxuan, HU Yanchun. Effect of cryptochlorogenic acid on immunomodulatory and antioxidant in mice of lung injury induced by H9N2-AIV[J]. Acta Agriculturae Zhejiangensis, 2022, 34(1): 33-40.

图/表 6

表1 小鼠脏器指数

Table 1 The organ index of mice

组别 Group	第3天 3rd day		第5天 5th day		第7天7th day
组别 Group	肺指数 Lung index	脾指数 Spleen index	肺指数 Lung index	脾指数 Spleen index	肺指数 Lung index	脾指数 Spleen index
H9N2	0.80±0.04 a	0.30±0.02 a	0.89±0.02 a	0.33±0.01 c	1.03±0.01 a	0.38±0.01 b
H9N2+4-CQA	0.76±0.05 a	0.32±0.03 a	0.79±0.03 b	0.36±0.01 b	0.80±0.02 b	0.44±0.04 ab
对照组Control	0.66±0.03 b	0.35±0.04 a	0.65±0.05 c	0.41±0.01 a	0.66±0.05 c	0.47±0.03 a
4-CQA	0.68±0.02 b	0.35±0.04 a	0.68±0.04 c	0.41±0.01 a	0.64±0.03 c	0.47±0.03 a

图1 小鼠肺组织病理学切片(A-J,200×)

Fig.1 Pathological changes of lung(A-J,200×)

表2 第7天各组小鼠脾T淋巴细胞亚群百分比及比值

Table 2 The percentage and ratio of T cell subgroups of mice on the 7th day

组别Group	CD4⁺/%	CD8⁺/%	CD4⁺/CD8⁺
H9N2	17.61±3.92 a	10.37±2.61 a	1.71±0.06 b
H9N2+4-CQA	19.20±3.73 a	8.75±2.03 a	2.21±0.08 a
对照组Control	19.74±4.77 a	9.52±2.16 a	2.07±0.03 a
4-CQA	16.34±3.54 a	7.51±1.51 a	2.20±0.15 a

图2 小鼠肺T-SOD活性变化 *,与空白对照组比较差异显著(P<0.05);#,与攻毒组比较差异显著(P<0.05)。下同。

Fig.2 Activity of T-SOD in lung of mice * showed the significant difference compared with that of control (P<0.05);# showed the significant difference compared with that of H9N2 group (P<0.05). The same as below.

图3 小鼠肺MDA含量变化

Fig.3 Content of MDA in lung of mice

图4 小鼠肺MPO活性变化

Fig.4 Activity of MPO in the lung of mice

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隐绿原酸对H9N2-AIV致小鼠肺损伤的免疫调节和抗氧化效果

Effect of cryptochlorogenic acid on immunomodulatory and antioxidant in mice of lung injury induced by H9N2-AIV

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